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Effects of Creatine and Exercise on Skeletal Muscle of FRG1-Transgenic Mice

Published online by Cambridge University Press:  02 December 2014

Daniel I. Ogborn ,
Katelyn J. Smith ,
Justin D. Crane ,
Adeel Safdar ,
Bart P. Hettinga ,
Rossella Tupler  and
Mark A. Tarnopolsky
Daniel I. Ogborn
Affiliation:
Department of Medical Sciences, McMaster University, Hamilton, Ontario, Canada
Katelyn J. Smith
Affiliation:
Department of Medical Sciences, McMaster University, Hamilton, Ontario, Canada
Justin D. Crane
Affiliation:
Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada
Adeel Safdar
Affiliation:
Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada
Bart P. Hettinga
Affiliation:
Department of Pediatrics and Medicine, McMaster University, Hamilton, Ontario, Canada
Rossella Tupler
Affiliation:
Department of Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
Mark A. Tarnopolsky*
Affiliation:
Department of Pediatrics and Medicine, McMaster University, Hamilton, Ontario, Canada
*
McMaster University Medical Centre, RM 2H26, 1200 Main St West, Hamilton, Ontario, L8N 3Z5, Canada.
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Abstract

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Background:

The FRG1-transgenic mouse displays muscle dysfunction and atrophy reminiscent of fascioscapulohumeral muscular dystrophy (FSHD) and could provide a model to determine potential therapeutic interventions.

Methods:

To determine if FRG1 mice benefit from treatments that improve muscle mass and function, mice were treated with creatine alone (Cr) or in combination with treadmill exercise (CrEX).

Results:

The CrEx treatment increased quadriceps weight, mitochondrial content (cytochome c oxidase (COX) activity, COX subunit one and four protein), and induced greater improvements in grip strength and rotarod fall speed. While Cr increased COX subunits one and four protein, no effect on muscle mass or performance was found. Since Cr resulted in no functional improvements, the benefits of CrEx may be mediated by exercise; however, the potential synergistic action of the combined treatment cannot be excluded.

Conclusion:

Treatment with CrEx attenuates atrophy and muscle dysfunction associated with FRG1 overexpression. These data suggest exercise and creatine supplementation may benefit individuals with FSHD.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 39 , Issue 2 , March 2012 , pp. 225 - 231
Copyright
Copyright © The Canadian Journal of Neurological 2012

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