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Tolerance and safety evaluation of N, N-dimethylglycine, a naturally occurring organic compound, as a feed additive in broiler diets

  • Isabelle D. Kalmar (a1) (a2), Martin W. A. Verstegen (a3), Klaus Maenner (a4), Jurgen Zentek (a4), Godelieve Meulemans (a5) and Geert P. J. Janssens (a1)...

Abstract

N, N-dimethylglycine (DMG) is a tertiary amino acid that naturally occurs as an intermediate metabolite in choline-to-glycine metabolism. The objective of the present trial was to evaluate tolerance, safety and bioaccumulation of dietary DMG in broilers when supplemented at 1 g and 10 g Na-DMG/kg. A feeding trial was conducted using 480 1-d-old broiler chicks that were randomly allocated to twenty-four pens and fed one of three test diets added with 0, 1 or 10 g Na-DMG/kg during a 39 d growth period. Production performance was recorded to assess tolerance and efficacy of the supplement. At the end of the trial, toxicity was evaluated by means of haematology, plasma biochemistry and histopathology of liver, kidney and heart (n 12), whereas bioaccumulation was assessed on breast meat, liver, blood, kidney and adipose tissue (n 8). Carcass traits were similar between the control and 1 g Na-DMG/kg feed groups (P>0·05), but the feed:gain ratio was significantly improved at 1 g Na-DMG/kg feed compared with the control or the 10-fold dose (P = 0·008). Histological examinations showed no pathological effects and results of haematology and plasma biochemistry revealed similar values between the test groups (P>0·05). Bioaccumulation occurred at the 10-fold dose, but the resulting DMG content in breast meat was comparable with, for instance, wheat bran and much lower than uncooked spinach. In conclusion, DMG at 1 g Na-DMG/kg improved the feed:gain ratio in broilers without DMG being accumulated in consumer parts. Furthermore, dietary supplementation with DMG up to 10 g Na-DMG/kg did not induce toxicity or impaired performance in broilers.

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Corresponding author

*Corresponding author: I. D. Kalmar, fax +32 264 78 48, email isabelle.kalmar@annureco.be

References

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1Tonda, ME & Hart, LL (1992) N, N-dimethylglycine and l-carnitine as performance enhancers in athletes. Ann Pharmacother 26, 935937.
2Currell, K, Ayed, A, Dziedzic, CE, et al. (2010) A–Z of nutritional supplements: dietary supplements, sports nutrition foods and ergogenic aids for health and performance – part 12. Br J Sports Med 44, 905907.
3Craig, SAS (2004) Betaine in human nutrition. Am J Clin Nutr 80, 539549.
4Garrow, TA (2001) Betaine-dependent remethylation. In Homocysteine in Health and Disease, pp. 145152 [Carmel, R and Jacobsen, DW, editors]. Cambridge: Cambridge University Press.
5Cupp, MJ & Tracy, TS (2003) Dimethylglycine (N, N-dimethylglycine). In Dietary Supplements: Toxicology and Clinical Pharmacology, pp. 149160 [Cupp, MJ and Tracy, TS, editors]. Totowa, NJ: Humana Press Inc.
6Kalmar, ID, Cools, A, Buyse, J, et al. (2010) Dietary N, N-dimethylglycine supplementation improves nutrient digestibility and attenuates pulmonary hypertension syndrome in broilers. J Anim Physiol Anim Nutr 94, e339e347.
7Kalmar, ID, Cools, A, Verstegen, MWA, et al. (2011) Dietary supplementation with dimethylglycine affects broiler performance and plasma metabolites depending on dose and dietary fatty acid profile. J Anim Physiol Anim Nutr 95, 146153.
8Dierick, NA & Decuypere, JA (2004) Influence of lipase and/or emulsifier addition on the ileal and faecal nutrient digestibility in growing pigs fed diets containing 4 % animal fat. J Sci Food Agric 84, 14431450.
9Cools, A, Maes, D, Buyse, J, et al. (2010) Effect of DMG supplementation in parturition feed for sows on metabolism, nutrient digestibility and reproductive performance. Animal 4, 20042011.
10Nahm, KH (2007) Feed formulations to reduce N excretion and ammonia emission from poultry manure. Bioresour Technol 98, 22822300.
11Kalmar, ID (2011) Efficacy and safety of dietary N,N-dimethylglycine in broiler production. PhD Thesis, Wageningen: Wageningen University.
12European Agency for the Evaluation of Medicinal Products (EMEA) (2001) Guideline on Environmental Impact Assessment for Veterinary Medicinal Products CVMP/VICH/592/98.
13European Community (EC) (2008) Commission Regulation (EC) No 429/2008 of 25 April 2008 on detailed rules for the implementation of Regulation (EC) No. 1831/2003 of the European Parliament and of the council as regards the preparation and the presentation of applications and the assessment and the authorisation of feed additives. Off J Eur Union L133, 165.
14Ausschuss für Bedarfsnormen der Gesellschaft für Ernährungsphysiologie (GfE) (1999) Energie-und Nährstoffbedarf landwirtschaftlicher Nutztiere. 7: Empfehlungen zur Energie- und Nährstoffversorgung von Masthühnern und Legehennen (Energy and Nutrient Requirements of Production Animals. 7: Recommendations on Energy and Nutrients for Broilers and Layers). Frankfurt: DLG-Verlag.
15Verband Deutscher Landwirtschaftlicher Untersuchungs und Forschungstantalten (VDLUFA) (1988) Methodenbuch Band 3 (Methodology Part 3). Darmstadt: VDLUFA-Verlag.
16Mongin, P (1981) Recent advantages in dietary anion–cation balance: applications in poultry. Proc Nutr Soc 40, 128136.
17Chendrimada, TP, Neto, MG, Pesti, GM, et al. (2002) Determination of the betaine content of feed ingredients using high-performance liquid chromatography. J Sci Food Agric 82, 15561563.
18Gascon, G, Patterson, B, Yearwood, K, et al. (1989) N, N-dimethylglycine and epilepsy. Epilepsia 30, 9093.
19Reap, EA & Lawson, JW (1990) Stimulation of the immune response by dimethylglycine, a nontoxic metabolite. J Lab Clin Med 115, 481486.
20López, KP, Schilling, MW & Corzo, A (2011) Broiler genetic strain and sex effects on meat characteristics. Poult Sci 90, 11051111.
21Mirghelenj, SA & Golian, A (2009) Effects of feed form on development of digestive tract, performance and carcass traits of broiler chickens. J Anim Vet Adv 8, 19111915.
22Borgatti, LMO, Albuquerque, R, Meister, NC, et al. (2004) Performance of broilers fed diets with different dietary electrolyte balance under summer conditions. Braz J Poult Sci 6, 153157.
23Jankowski, J, Zdunczyk, Z, Juskiewicz, J, et al. (2011) The effect of different dietary sodium levels on the growth performance of broiler chickens, gastrointestinal function, excreta moisture and tibia ash. J Anim Feed Sci 20, 93106.
24Abdullah, AY, Al-Beitawi, NA, Rjoup, MMS, et al. (2010) Growth performance, carcass and meat quality characteristics of different commercial crosses of broiler strain chickens. J Poult Sci 47, 1321.
25Kubena, LF, Chen, TC, Deaton, JW, et al. (1974) Factors influencing the quantity of abdominal fat in broilers. 3. Dietary energy levels. Poult Sci 53, 974978.
26Apo, MM (2008) Appendix 2: hematology reference values. In Avian Medicine, 2nd ed., pp. 420436 [Samour, J, editor]. Edingburgh: Elsevier Limited.
27Gross, WB & Siegel, HS (1983) Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Dis 27, 972979.
28Maxwell, MH (1993) Avian blood leucocyte responses to stress. World's Poult Sci J 49, 3443.
29Danishefsky, I (1980) Biochemistry for Medical Sciences. Boston: Little, Brown and Company.
30Hoffmann, WE, Kramer, J, Main, AR, et al. (1989) Clinical enzymology. In The Clinical Chemistry of Laboratory Animals, pp. 237278 [Loeb, WF and Quimby, FW, editors]. New York, NY: Pergamon Press.
31Jaensch, MJ, Cullen, L & Raidal, SR (2000) Assessment of liver function in galahs/cockatoos (Eolophus roseicapillus) after partial hepatectomy: a comparison between plasma enzyme concentrations, serum bile acid levels, and galactose clearance tests. J Avian Med Surg 14, 164171.
32Harr, KE (2002) Clinical chemistry of companion avian species: a review. Vet Clin Pathol 31, 140151.
33Samour, J (2008) Clinical and diagnostic procedures. In Avian Medicine, pp. 31135 [Samour, J, editor]. Edinburgh: Elsevier Limited.
34Hodges, RD (1974) The liver. In The Histology of the Fowl, pp. 88101 [Hodges, RD, editor]. London: Academic Press Inc.
35Maxwell, MH, Robertson, GW & Spence, S (1986) Studies on an ascetic syndrome in young broilers. 1. Haematology and pathology. Avian Pathol 15, 511524.
36Crespo, R & Shivaprasad, HL (2003) Developmental, metabolic, and other non-infectious disorders. In Diseases of Poultry, 11th ed., pp. 10551102 [Saif, YM, editor]. Iowa, IA: Iowa State Press.
37Doupnick, B & Peckham, JC (1970) Mycotoxicity of Aspergillus ochraceus to chicks. Appl Microbiol 19, 594597.
38Wheeler, MB & Campion, DR (1993) Animal production – a longstanding biotechnological success. Am J Clin Nutr 58, 276S281S.
39Bird, HR (1961) Additives and residues in foods of animal origin. Am J Clin Nutr 9, 260268.
40Anonymous (1996) N, N-dimethylglycine. In The Merck Index, 12th ed. [Budavari, S, O'Neil, MJ, Smith, A, Heckelman, PE and Kinneary, JF, editors]. Whitehouse Station: Merck Research Laboratories.
41Lever, M, Atkinson, W, Slow, S, et al. (2009) Plasma and urine betaine and dimethylglycine variation in healthy young male subjects. Clin Biochem 42, 706712.
42Slow, S, McGregor, DO, Lever, M, et al. (2004) Dimethylglycine supplementation does not affect plasma homocysteine concentrations in pre-dialysis chronic renal failure patients. Clin Biochem 37, 974976.
43Larbier, M & Leclercq, B (1992) Nutrition et alimentation des volailles (Nutrition and Feeding of Poultry). Paris: INRA.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
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