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Riboflavin deficiency, metabolic rate and brown adipose tissue function in sucking and weanling rats

Published online by Cambridge University Press:  09 March 2007

B. E. Patterson  and
C. J. Bates
B. E. Patterson
Affiliation:
MRC Dunn Nutritional Laboratory, Downham's Lane, Milton Road, Cambridge CB4 1XJ
C. J. Bates
Affiliation:
MRC Dunn Nutritional Laboratory, Downham's Lane, Milton Road, Cambridge CB4 1XJ
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Abstract

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1. The effects of riboflavin deficiency on growth, whole-body oxygen consumption, cytochrome c oxidase (EC 1.9.3.1) activity and GDP-binding capacity of brown adipose tissue were measured in three groups of rats: sucking pups, weanling rats, and dams. Control groups were weight-matched, pair-fed or fed ad lib.

2. Riboflavin deficiency reduced growth rate and increased the activation coefficient of erythrocyte glutathione reductase (NAD(P)H) (EC 1.6.4.2), as predicted. In sucking pups it also reduced whole-body O2 consumption per unit body-weight, especially after noradrenaline stimulation. In weanling rats, however, it increased O2 consumption both before and after noradrenaline stimulation.

3. Cytochrome c oxidase (EC 1.9.9.1) activity of brown adipose tissue was not consistently affected by riboflavin deficiency. Binding of [3H]GDP to the mitochondria was increased in the deficient weanling rats.

4. Weanling rats therefore, seemed better able to withstand the effects of severe depletion. Their reduced growth and increased non-shivering thermogenesis helped to counteract the unfavourable ratio of riboflavin: other tissuebuilding materials. The relevance for thermoregulation in riboflavin-deficient children is discussed.

Type
Energy Metabolism
Information
British Journal of Nutrition , Volume 61 , Issue 3 , May 1989 , pp. 475 - 483
Copyright
Copyright © The Nutrition Society 1989

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