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Protein deficiency, but not zinc deficiency, reduces recovery of type 1 and type 2 muscle fibre diameters in the gastrocnemius muscle of growing rats

  • Alexia L. V. Prescod (a1), William C. Halliday (a2) (a3) and Carla G. Taylor (a1)

Abstract

The present study examines the effects of protein- and energy-type malnutrition in combination with Zn deficiency on the growth, serum insulin-like growth factor-1 (IGF-1), gastrocnemius muscle mass and fibre diameter of growing rats during a deficiency phase followed by nutritional rehabilitation. Rats (3-weeks old) were randomly assigned to baseline, or Zn-deficient (Z, < 1 mg Zn/kg), protein-deficient (P, 20 g protein/kg), combined Zn- and protein-deficient (ZP), energy-deficient (E, feed intake pair-fed to Z) or control (C, 30 mg Zn/kg and 170 g protein/kg) groups for a 3-week deficiency phase, followed by a 3-week repletion phase with the control diet. ATPase histochemical staining at pH 9·4 was used to differentiate type 1 and type 2 muscle fibres. After the deficiency phase, the ZP and P groups had lower body weight and smaller gastrocnemius muscle mass than the Z and E groups. Type 1 and 2 muscle fibre diameters (T1- and T2-MFD, respectively) were reduced in the ZP, P and Z groups compared with the E and C groups. Serum Zn was reduced in the ZP, P and Z groups, but serum IGF-1 was lowest in the Z and E groups. After the repletion phase, T1-MFD did not recover in the P and E groups nor T2-MFD in the P group, despite the P and E groups having a better recovery of body weight. In summary, previous protein deficiency, but not Zn deficiency, limited the recovery of both T1- and T2-MFD during nutritional repletion. The quality of skeletal muscle recovery in the malnourished groups was not associated with body weight, muscle mass, serum Zn or IGF-1 concentrations.

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      Protein deficiency, but not zinc deficiency, reduces recovery of type 1 and type 2 muscle fibre diameters in the gastrocnemius muscle of growing rats
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Corresponding author

*Corresponding author: Dr C. G. Taylor, fax +1 204 237 4018, email ctaylor@cc.umanitoba.ca

References

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1World Health Organization (2009) Global Health Risks. Mortality and Burden of Disease Attributable to Selected Major Risks. Geneva: WHO.
2Graham, GG, Cordano, A, Blizzard, RM, et al. (1969) Infantile malnutrition: changes in body composition during rehabilitation. Pediatr Res 3, 579589.
3MacLean, WC & Graham, GG (1980) The effect of energy intake on nitrogen content of weight gained by recovering malnourished infants. Am J Clin Nutr 33, 903909.
4Montgomery, RD (1962) Muscle morphology in infantile protein malnutrition. J Clin Pathol 15, 511521.
5Hansen-Smith, FM, Picou, D & Golden, MH (1979) Growth of muscle fibres during recovery from severe malnutrition in Jamaican infants. Br J Nutr 41, 275282.
6Golden, MHN & Golden, BE (1981) Plasma Zn, rate of weight gain, and the energy cost of tissue deposition in children recovering from severe malnutrition on cow's milk or soya protein based diet. Am J Clin Nutr 34, 892899.
7Golden, MHN & Golden, BE (1981) Effect of zinc supplementation on the dietary intake, rate of weight gain, and energy cost of tissue deposition in children recovering from severe malnutrition. Am J Clin Nutr 34, 900908.
8Golden, BE & Golden, MHN (1992) Effects of zinc on lean tissue synthesis during recovery from malnutrition. Eur J Clin Nutr 46, 697706.
9Morgan, PN, Keen, CL, Calvert, CC, et al. (1988) Effect of varying dietary zinc intake of weanling mouse pups during recovery from early undernutrition on growth, body composition and composition of gain. J Nutr 118, 690698.
10Glore, SR, Orth, VL, Knehans, AW, et al. (1993) Efficacy of dietary zinc supplementation on catch-up growth after protein malnutrition. J Nutr Biochem 4, 281285.
11Jennische, E & Olivercrona, H (1987) Transient expression of insulin-like growth factor-1 immunoreactivity in skeletal muscle cells during postnatal development in the rat. Acta Physiol Scand 131, 619622.
12Zanconato, S, Moromisato, DY, Moromisato, MY, et al. (1994) Effect of training and growth hormone suppression on insulin-like growth factor-1 mRNA in young rats. J Appl Physiol 76, 22042209.
13Adams, GR & Haddad, F (1996) The relationships among IGF-1, DNA content and protein accumulation during skeletal muscle hypertrophy. J Appl Physiol 81, 25092516.
14Cesur, Y, Yordaman, N & Doğan, M (2009) Serum insulin-like growth factor-I and insulin-like growth factor binding protein-3 levels in children with zinc deficiency and the effect of zinc supplementation on these parameters. J Pediatr Endocrinol Metab 22, 11371143.
15Akman, I, Arioglu, P, Koroglu, OA, et al. (2006) Maternal zinc and cord blood zinc, insulin-like growth factor-1, and insulin-like growth factor binding protein-3 levels in small-for-gestational-age newborns. Clin Exp Obstet Gynecol 33, 238240.
16Doherty, CP, Crofton, PM, Sarkar, MA, et al. (2002) Malnutrition, zinc supplementation and catch-up growth: changes in insulin-like growth factor I, its binding proteins, bone formation and collagen turnover. Clin Endocrinol (Oxf) 57, 391399.
17Prewitt, TE, D'Ercole, AJ, Switzer, BR, et al. (1982) Relationship of serum immunoreactive somatomedin-C to dietary protein and energy in growing rats. J Nutr 112, 144150.
18Thissen, JP, Underwood, LE, Maiter, D, et al. (1991) Failure of insulin-like growth factor-1 (IGF-1) infusion to promote growth in protein-restricted rats despite normalization of serum IGF-1 concentrations. Endocrinology 128, 885890.
19Cossack, ZT (1986) Somatomedin-C and zinc status in rats as affected by zinc, protein and food intake. Br J Nutr 56, 163169.
20Dorup, I, Flyvbjerg, A, Everts, ME, et al. (1991) Role of insulin-like growth factor-1 and growth hormone in growth inhibition induced by magnesium and zinc deficiencies. Br J Nutr 66, 505521.
21Roth, HP & Kirchgessner, M (1994) Influence of alimentary zinc deficiency on the concentration of growth hormone (GH), insulin-like growth factor-1 (IGF-1) and insulin in the serum of force-fed rats. Horm Metab Res 26, 404408.
22Ketelslegers, JM, Maiter, D, Maes, M, et al. (1995) Nutritional regulation of insulin-like growth factor-1. Metabolism 44, 5057.
23Mares-Perlman, JA, Subar, AF, Block, G, et al. (1995) Zinc intake and sources in the US adult population: 1976–1980. J Am Coll Nutr 14, 349357.
24Sarnat, HB (1983) Muscle Pathology and Histochemistry. Chicago, IL: American Society of Clinical Pathologists Press.
25Reeves, PG, Nielsen, FH & Fahey, GC (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123, 19391951.
26Canadian Council on Animal Care (1993) Guide to the care and use of experimental animals. http://www.ccac.ca/en/CCAC_Programs/Guidelines_Policies/GUIDES/ENGLISH/toc_v1.htm (accessed 5 July 2010).
27Dubowitz, V & Brooke, MH (1973) Muscle Biopsy: A Modern Approach. Toronto: WB Saunders Co Ltd.
28Estes, ML & Goss, GR (1996) Muscle Biopsy: the Role of the Technologist in Optimum Freezing, Enzyme Histochemistry and Diagnosis. Canadian Society of Laboratory Technologists Congress Winnipeg, Canada.
29Lepage, LM, Giesbrecht, J & Taylor, CG (1999) Expression of T lymphocyte p56lck, a zinc-finger signal transduction protein, is elevated by dietary zinc deficiency and diet restriction in mice. J Nutr 129, 620627.
30Backeljauw, P, Bang, P, Dunger, DB, et al. (2010) Insulin-like growth factor-I in growth and metabolism. J Pediatr Endocrinol Metab 23, 316.
31Glore, SR & Layman, DK (1987) Cellular development of skeletal muscle of rats during recovery from prolonged undernutrition. J Nutr 117, 17671774.
32Oldfors, A, Mair, WGP & Sourander, P (1983) Muscle changes in protein-deprived young rats. A morphometrical, histochemical, and ultrastructural study. J Neurol Sci 59, 291302.
33Lee, S, Chanoit, G, McIntosh, R, et al. (2009) Molecular mechanism underlying Akt activation in zinc-induced cardioprotection. Am J Physiol Heart Circ Physiol 297, H569H575.
34Halevy, O, Geyra, A, Barak, M, et al. (2000) Early posthatch starvation decreases satellite cell proliferation and skeletal muscle growth in chicks. J Nutr 130, 858864.
35Peterson, JM, Bryner, RW & Always, SE (2008) Satellite cell proliferation is reduced in muscles of obese Zucker rats but restored with loading. Am J Physiol Cell Physiol 295, C521C528.

Keywords

Protein deficiency, but not zinc deficiency, reduces recovery of type 1 and type 2 muscle fibre diameters in the gastrocnemius muscle of growing rats

  • Alexia L. V. Prescod (a1), William C. Halliday (a2) (a3) and Carla G. Taylor (a1)

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