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        Olive oil intake and CHD in the European Prospective Investigation into Cancer and Nutrition Spanish cohort
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Olive oil is well known for its cardioprotective properties; however, epidemiological data showing that olive oil consumption reduces incident CHD events are still limited. Therefore, we studied the association between olive oil and CHD in the European Prospective Investigation into Cancer and Nutrition (EPIC) Spanish cohort study. The analysis included 40 142 participants (38 % male), free of CHD events at baseline, recruited from five EPIC-Spain centres from 1992 to 1996 and followed up until 2004. Baseline dietary and lifestyle information was collected using interview-administered questionnaires. Cox proportional regression models were used to assess the relationship between validated incident CHD events and olive oil intake (energy-adjusted quartiles and each 10 g/d per 8368 kJ (2000 kcal) increment), while adjusting for potential confounders. During a 10·4-year follow-up, 587 (79 % male) CHD events were recorded. Olive oil intake was negatively associated with CHD risk after excluding dietary mis-reporters (hazard ratio (HR) 0·93; 95 % CI 0·87, 1·00 for each 10 g/d per 8368 kJ (2000 kcal) and HR 0·78; 95 % CI 0·59, 1·03 for upper v. lower quartile). The inverse association between olive oil intake (per 10 g/d per 8368 kJ (2000 kcal)) and CHD was more pronounced in never smokers (11 % reduced CHD risk (P = 0·048)), in never/low alcohol drinkers (25 % reduced CHD risk (P < 0·001)) and in virgin olive oil consumers (14 % reduced CHD risk (P = 0·072)). In conclusion, olive oil consumption was related to a reduced risk of incident CHD events. This emphasises the need to conserve the traditional culinary use of olive oil within the Mediterranean diet to reduce the CHD burden.

The favourable effect of olive oil on CHD has long been suggested, as international comparisons noted that CHD mortality and incidence rates were lower in countries where olive oil consumption was higher(13). In addition, large cohort studies set in Mediterranean countries have reported that the traditional Mediterranean diet (MD) reduces risk of CHD mortality(4) and incidence(5, 6), CVD risk factors(7, 8) and also secondary CHD events(9). Although it is difficult to isolate the contribution of individual components within this dietary pattern, cumulative evidence suggests that olive oil, a key feature of the MD and the principal source of fat, plays an important role in these health benefits(8, 1013). However, there are currently very few studies showing that consuming olive oil prevents incident CHD events.

For instance, case–control studies assessing the relationship between CHD and olive oil directly (not using MUFA as a proxy) are limited and conflicting, and have indicated inverse associations(14, 15) or no association(16). The Italian EPICOR study(17), to our knowledge the only cohort study to date that has prospectively studied olive oil and CHD risk (but only in women), showed that olive oil intake in the upper quartile reduced risk of incident CHD events by 44 % (P-trend 0·04).

Although intervention trials support the evidence that olive oil reduces CHD risk factors(7, 18, 19), there are still no results on primary CHD endpoints. However, experimental studies shed light on the biological mechanisms behind olive oil's cardio-protective role, attributing these benefits to its high MUFA oleic acid content (55–83 % of total fatty acids) and to its highly bioactive micro-components (1–2 % of total content)(10). Most heavily implicated are its polyphenolic compounds, in particular hydroxytyrosol(20), which is found in high concentrations in extra-virgin olive oil. Polyphenols have been shown to have a broad spectrum of benefits including anti-inflammatory, antioxidant, anti-arrhythmic, anti-atherogenic and vasodialatory effects, favouring a less pro-thrombotic environment and greater endothelial protective capacity(8, 19).

Although it is clear that olive oil improves many CHD risk factors, there is still limited evidence corroborating its role in preventing incident CHD events. Accordingly, recent reviews on olive oil and CVD(10, 11, 21) have concluded that further prospective studies and trials are needed to obtain more complete evidence for the primary prevention of CHD. Therefore, we aimed to prospectively study the relationship between olive oil consumption and risk of incident CHD events in the European Prospective Investigation into Cancer and Nutrition (EPIC)-Spain cohort study.


Study design and population

The EPIC is a large multi-centric European prospective cohort study designed to study the relationship between nutritional, lifestyle, metabolic and genetic factors and risk of cancer and other chronic diseases. The full methodological details have been published previously(22, 23). The present study makes use of the Spanish cohort of EPIC-Heart, the cardiovascular component of EPIC(24). EPIC-Spain consists of 41 438 healthy volunteers (38 % male), aged 29–69 years, recruited from 1992 to 1996 from five Spanish regions (Asturias, Granada, San Sebastian, Murcia and Navarra). Most participants (75 %) were blood donors, and the study population covered a wide range of socio-economic and educational levels. At recruitment, participants signed an informed consent and the study was approved by the Ethics Committee of the Bellvitge Hospital (Barcelona).

Dietary and lifestyle questionnaires

All centres used an interview-administered computerised version of a dietary history questionnaire to collect information on usual food intake over the previous 12 months(25). The validated dietary questionnaire was open, but was structured by occasions of intake and included a list of 662 common foods and recipes from each region(25, 26). The portions of each food consumed (including oil added to the salads and cooked foods (g/d)) were quantified using household and standard measures and thirty-five sets of pictures with simple foods, food mixtures and drinks(25). A specific food composition table was used to calculate each participant's total daily energy and nutrient intake(27). At recruitment, weight, height and waist circumference were measured and information on socio-demographic and lifestyle factors, including educational attainment, tobacco use, alcohol consumption, reproductive and medical history and physical activity was collected through interview-administered questionnaires(22, 28).

Ascertainment and validation of CHD events

Incident CHD events were identified during a follow-up period that ran from recruitment until December 2004 (full details have been published previously(5)). Briefly, non-fatal and fatal coronary events were identified through the self-reported questionnaires at recruitment and at 3-year follow-up (all centres) and by record linkage with three sources of information (varying by centre): hospital discharge databases (Granada had limited access), population-based myocardial infarction (MI) registries and regional and national mortality registries (National Statistical Institute). All incident CHD events were validated by trained nurses/physicians who reviewed medical records and medico-legal autopsy reports to confirm and classify the events according to the American Heart Association Scientific Statement of 2003(29) and the multinational monitoring of trends and determinants in CVD (Multinational MONItoring of trends and determinants in CArdiovascular disease; MONICA) criteria.

Coronary events were classified as definite (fatal or non-fatal acute MI or unstable angina requiring revascularisation procedures such as coronary artery bypass graft or percutaneous transluminal coronary angioplasty) or possible (fatal or non-fatal MI that did not meet all diagnostic criteria and fatal CHD with insufficient information). Possible CHD events (n 101) were not considered as cases but were censored at the time of their coronary event. Participants with a definite coronary event recorded before recruitment were considered as prevalent cases and excluded.

Study exclusions and final sample

Of the initial 41 438 participants, 193 were excluded due to prevalent CHD, 806 for extreme total energy intake (lowest and highest 1 % of the total energy intake to energy requirement ratio) and 297 for missing information on date of possible CHD event (n 12), hyperlipidaemia (n 195), diabetes (n 71) or hypertension (n 60). Thus, the final sample used for the analyses included 40 142 participants without history of coronary events at recruitment.

Statistical analysis

Olive oil is presented as a function of energy density (g/d per 8368 kJ (2000 kcal), excluding energy from alcohol)(30). Participants' baseline characteristics were described according to olive oil intake using mean and standard deviation and frequencies (%), and differences were tested using Kruskal–Wallis rank sum and χ2 tests, respectively. The association between olive oil intake and CHD was assessed using Cox proportional hazards regression models. The models were stratified by age at recruitment (1-year intervals), sex and centre. Age was the primary time variable, with entry time defined as age at recruitment and exit time defined as age at diagnosis of first CHD event for cases and age at censoring or at death (whichever occurred first) for at-risk participants.

Olive oil was analysed as a categorical variable (quartiles) and a continuous variable (per 10 g/d per 8368 kJ (2000 kcal)). Linear trend tests were calculated for the categorical variable using the median of each quartile as a continuous variable. Four different models were created, the first was unadjusted, whereas the second model was partially adjusted for known CHD risk factors such as: BMI ( < 25, 25–30 and ≥ 30 kg/m2), waist circumference (cm), educational level (none, primary school, secondary school, technical or professional training, university degree and not specified), smoking status (never, former, current ≤ 20 cigarettes/d, current >20 cigarettes/d and missing), physical activity level measured as total metabolic equivalents (MET)/h per week of recreational exercise (inactive, moderately inactive, moderately active and active), energy intake without alcohol (kJ/d), baseline alcohol intake (0, >0 to < 6, ≥ 6 to < 12, ≥ 12 to < 24 and ≥ 24 g/d) and the presence of diabetes, hyperlipidaemia and hypertension (yes, no). Diabetes, hyperlipidaemia and hypertension were self-reported at recruitment or based on drug use. The third model (fully adjusted) was also adjusted for a MD score (full methodology has been described previously(5)) which included fruit, vegetables, cereals, fish, legumes, dairy products and meat but excluded olive oil and alcohol (14-unit, continuous variable). The final model (Goldberg exclusions) excluded participants with a poor concordance of energy intake to energy expenditure, identified using Goldberg criteria(31), from the fully adjusted model.

Subgroup analyses were carried out to evaluate CHD risk by type of olive oil intake: participants who consumed ordinary olive oil v. participants who exclusively consumed virgin olive oil (16 % of olive oil consumers). Stratified analyses were also run by sex (female models were additionally adjusted for menopausal status (pre- and peri-menopausal and post-menopausal)), smoking status (ever v. never), waist circumference (men < 102 cm and women < 88 cm v. men ≥ 102 cm and women ≥ 88 cm) defined according to the National Cholesterol Education Program-Adult Treatment Panel III cut-offs(32) and lifetime alcohol consumption (never or light (0–5·9 g/d), moderate (6–23·9 g/d) and heavy ( ≥ 24 g/d) alcohol intake). Likelihood ratio tests were used to test for possible interaction between these variables and olive oil.

Sensitivity analyses were carried out by excluding the first 2 years of follow-up, subjects with hyperlipidaemia, hypertension and diabetes at recruitment and those from Granada (due to possible incomplete CHD events ascertainment). Schoenfeld residuals were used to ensure the proportional hazards assumption. All analyses were performed using STATA statistical software, version 10 (StataCorporation 2007) and statistical significance was set at P < 0·05.


The distribution of the 40 142 participants (38 % male) across the five EPIC-Spain centres is shown in Table 1. During a mean follow-up of 10·4 (sd 1·3) years, 587 (79 % male) incident CHD events were recorded. The mean olive oil intake in the whole cohort was 20·1 g/d per 8368 kJ (2000 kcal), which was highest in Navarra and Granada and lowest in Asturias (Table 1). A total of 14·8 % of the participants did not consume olive oil.

Table 1 The distribution of participants, CHD and olive oil intake in the five centres of the European Prospective Investigation into Cancer and Nutrition (EPIC)-Spain cohort study (Medians, percentages and percentiles)

P25, 25th percentile; P75, 75th percentile.

* Crude CHD rates calculated per 100 000 person-years.

Granada did not fully validate/ascertain CHD events in the cohort.

The baseline characteristics of the cohort are described according to quartiles of olive oil intake in Table 2. Participants with a higher intake of olive oil were more likely to have a secondary school education or above, be hyperlipidaemic, have a narrower waist circumference, a lower energy intake and a higher MD score.

Table 2 Participants' baseline characteristics according to olive oil intake (Mean values and standard deviations; number of participants and percentages)

* Apart from diabetes, all comparisons between baseline characteristics and olive oil intake (χ2 or Kruskal–Wallis rank sum test) showed significant association at P < 0·05.

For women only.

Excluding alcohol and olive oil.

The hazard ratios (HR) for the association between olive oil and CHD events are shown in Table 3. Olive oil intake was inversely associated with CHD, but this association weakened after adjusting for lifestyle and dietary-related confounders. However, after excluding participants with poor concordance of energy intake to energy expenditure (Goldberg exclusions), there was a 22 % decreased risk of a CHD for the upper v. the lower olive oil quartile and a 7 % (P = 0·050) reduction in incident CHD for each 10 g/d per 8368 kJ (2000 kcal) of olive oil.

Table 3 Multivariate hazard ratios (HR) for incident CHD events according to intake of olive oil in the European Prospective Investigation into Cancer and Nutrition (EPIC)-Spain cohort (Hazard ratios (HR) and 95 % confidence intervals)

* Obtained from Cox regression models stratified by age at recruitment, centre and sex.

Models adjusted for educational level, BMI, waist circumference, physical activity, smoking status, alcohol consumption, energy intake excluding alcohol, hyperlipidaemia, hypertension and diabetes.

Models additionally adjusted for the Mediterranean diet score (excluding olive oil and alcohol).

§ Models adjusted as in fully adjusted but excluding 13 592 participants (171 cases; quartile 1 = 151 cases, quartile 2 = 79 cases, quartile 3 = 92 cases, quartile 4 = 94 cases) with improbable dietary values according to Goldberg criteria.

Table 4 presents the results of the association between olive oil (each 10 g/d per 8368 kJ (2000 kcal) increment) and CHD events within different subgroups. After stratifying by type of olive oil intake, there appeared to be a greater decreased risk of a CHD event for consumption of virgin compared to ordinary olive oil (HR 0·86; 95 % CI 0·72, 1·01 and HR 0·97; 95 % CI 0·91, 1·03, respectively, for each 10 g/d per 8368 kJ (2000 kcal)). Although the inverse association was greater in females compared to males, the test for interaction was not significant. Olive oil intake was associated with a reduced risk of CHD in never smokers (HR 0·89; 95 % CI 0·80, 1·00) but not in ever smokers. There was evidence of effect modification by lifetime alcohol intake, as there was an important significant negative association in never or light alcohol drinkers, with a 25 % decreased risk of CHD (P < 0·001) for each 10 g/d of olive oil consumption, while there was no evidence of an association in moderate or heavy drinkers (P-interaction = 0·004).

Table 4 Association between olive oil consumption and CHD in population subgroups in the European Prospective Investigation into Cancer and Nutrition (EPIC)-Spain cohort (Hazards ratios (HR) and 95 % confidence intervals)

N/A, not applicable.

* Models stratified by age, centre and sex, and adjusted for educational level, BMI, waist circumference, physical activity, smoking status, alcohol consumption, energy intake excluding alcohol, hyperlipidaemia, hypertension and diabetes and Mediterranean diet score (and menopausal status in the female model).

Smoking status excludes twenty-one participants with unknown smoking status.

Waist circumference cut-offs correspond to the National Cholesterol Education Program's Adult Treatment Panel III criteria.

§ Excluding alcohol and olive oil.

In sensitivity analyses (data not shown), after excluding the first 2 years of follow-up, subjects with hyperlipidaemia, hypertension and diabetes at recruitment and those from the centre of Granada, the association did not materially change, i.e. HR 0·96 (95 % CI 0·91, 1·03), HR 0·93 (95 % CI 0·84, 1·02) and HR 0·97 (95 % CI 0·91, 1·03), respectively, for each 10 g/d per 8368 kJ (2000 kcal).


To our knowledge, this is the largest cohort study to assess the relationship between olive oil consumption and incident CHD events. The present results in a Mediterranean population provide suggestive evidence that a greater consumption of olive oil was associated with a decreased risk of incident CHD events; after excluding participants with a poor concordance of energy intake to energy expenditure, there was a significant (7 %) reduction in CHD risk for each 10 g/d per 8368 kJ (2000 kcal) of olive oil and a non-significant (22 %) reduction for consumption in the upper quartile. This suggested that the protective effect may have been driven by the consumption of virgin olive oil, although further studies need to corroborate this.

The present results are in line with previous observational studies in Spain, Greece and Italy(14, 15, 17), although we observed a more modest reduction in CHD risk. In a Spanish hospital-based case–control study(14), the top quartile of energy-adjusted olive oil intake was associated with a 82 % (P = 0·03) reduction in a first acute MI, and in a Greek hospital-based case–control study(15), exclusive olive oil users were 47 % less likely to have an acute coronary syndrome compared to non-users. An Italian cohort study reported a 44 % decreased risk of CHD in women whose olive oil consumption was in the upper quartile (>31·2 g/d)(17). Less direct evidence comes from studies investigating mortality in patients with a previous MI. For instance, the Italian GISSI (Gruppo Italiano per lo Studio della Sopravvivenza nell'Infarto miocardio) Prevenzione trial showed that regular olive oil consumption significantly reduced mortality in MI patients by 24 %(12). Although the EPIC-Greece study(13) did not find a significant association between each 21 g/d increment in olive oil and mortality in MI patients, there was a significant (18 %) reduction in mortality patients with a high MUFA:SFA ratio.

Differences in study designs and adjustment for confounding variables are likely to contribute to the differences in the magnitude of effect observed between studies. Olive oil intake is an indicator of a healthy MD and is usually correlated with foods such as vegetables; not adjusting for dietary factors may have led to an overestimation of the association. In addition, part of these differences might be explained by the type of olive oil (virgin v. ordinary) consumed across populations.

The present results are supported by strong mechanistic evidence(8, 10) and results from experimental studies show that consuming olive oil improves important CHD risk factors. The PREDIMED (PREvención con DIeta MEDiterránea) dietary intervention trial has demonstrated that a MD enriched with extra virgin olive oil benefits blood pressure, glycaemic control in diabetics, endothelial function, oxidative stress and lipid profiles (decreasing TAG, increasing HDL- and lowering total and LDL-cholesterol) and reduces susceptibility of LDL to oxidation and concentrations of inflammatory markers such as C-reactive protein and IL-6(7, 33, 34). In addition, the olive oil-rich diet was effective in the prevention of diabetes, the metabolic syndrome and weight gain(35, 36). The wide number of pathways by which olive oil intake could decrease CHD make a causal relationship probable.

Olive oil's benefits have been related to its high content of MUFA and its richness in bioactive micro-components such as polyphenols, abundant in virgin and extra-virgin olive oil but not in ordinary olive oil(8, 10, 20, 37). The EUROLIVE dietary intervention study showed that in vivo consumption of olive oil with three different phenolic concentrations increased HDL-cholesterol and decreased total and LDL-cholesterol, TAG and oxidative stress markers in a dose-dependent manner(10, 38). A key olive oil polyphenol is oleuropein (a compound that generates tyrosol and hydroxytyrosol), which accounts for approximately 80 % of olive oil phenolic content and is a potent scavenger of superoxide radicals and inhibits LDL oxidation(19, 39). The greater inverse association observed between olive oil and CHD risk when focusing on virgin olive oil consumers is consistent with the fact that olive oil is not just a MUFA and that other biologically active components are of importance.

The negative association between olive oil and CHD was more apparent in never or low alcohol drinkers and never smokers. As moderate alcohol intake is known to protect against CHD(40), the fact that the beneficial effect of olive oil on CHD was only apparent in never or light drinkers could be because the important protective effect of alcohol in moderate or heavy drinkers masked the more subtle effect of olive oil, although this remains a hypothesis. Along the same line, the greater risk of CHD in smokers may have obscured any beneficial effect of olive oil and explain the differences observed between ever and never smokers. The inverse association was also stronger in women and could be explained by less olive oil intake misclassification error. Spanish women belonging to the generation included in the present cohort, recruited from 1992 to 1996, were usually responsible for preparing meals, and may have provided more accurate information on the quantity and type of oils used.

The strengths of the present study include its long follow-up period, large sample of initially healthy individuals and inclusion of validated incident definite CHD events. In addition, the mean olive oil intake in this Mediterranean population was relatively high, with 20·1 g/d per 8368 kJ (2000 kcal) overall and 38 g/d per 8368 kJ (2000 kcal) in the highest quartile of intake. We used the multivariate nutrient density model(30) to adjust for total energy intake and adjusted for adherence to the MD to separate the independent effect of olive oil on CHD from the rest of the MD components. In addition, we were able to explore the difference in effects between ordinary and virgin olive oil, which is seldom assessed in observational studies. Finally, we took into account possible dietary mis-reporting, applying a methodology identifying disparities between reported energy intakes and estimated requirements(41). Interestingly, the association strengthened after restricting the analysis to those participants with more reliable dietary intakes.

The present study has some limitations too, such as although we adjusted for many lifestyle factors strongly related with CHD, we cannot rule out residual confounding. Also, olive oil and other dietary intakes were assessed using a single measurement taken at baseline and any changes during follow-up would not have been captured. In addition, participants with pre-clinical medical conditions at recruitment might have already changed their habitual diet to a healthier or olive-oil-richer diet, which could lead to reverse causality or dilute the real association. However, sensitivity analyses excluding the first 2 years of follow-up and participants with hyperlipidaemia, hypertension or diabetes showed similar results. Fewer cases of CHD were documented in Granada because of limited access to sources of ascertainment and incomplete follow-up, but excluding Granada from the main analyses resulted in minimal changes. Finally, some measurement error is unavoidable in nutritional epidemiology and it is especially difficult to accurately quantify added fats. However, the interview-administered validated dietary history questionnaire(25) used in the present study has been shown to have less measurement error than FFQ(42). In addition, measurement error tends to introduce non-differential exposure misclassification, leading to effect dilution.

In conclusion, the results from this large Mediterranean cohort provide some evidence that olive oil consumption is associated with a reduction in primary incident CHD events. Previously published results in this cohort reported that high adherence to the entire MD was associated with a 40 % decreased risk of incident CHD events(5). The present study therefore supports the contribution that olive oil makes within this dietary pattern in terms of reducing risk of CHD events. Our findings back the need to preserve the culinary use of olive oil within the Mediterranean dietary tradition.


The authors declare no conflict of interest. The present work was supported by the Spanish Ministry of Health (ISCIII RETICC RD06/0020); Spanish Regional Governments of Andalusia, Asturias, Basque Country, Murcia (no. 6236) and Navarra and the Catalan Institute of Oncology.

All authors have contributed towards the different phases of the manuscript preparation (the study design, interpretation of the results and content of the manuscript text and table layout) and also approved the final submitted version. G. B. and N. T. were responsible for the statistical analysis, for writing the manuscript and compiling the tables and C. A. G. is the guarantor.


1Tunstall-Pedoe, H, Kuulasmaa, K, Mahonen, M, et al. (1999) Contribution of trends in survival and coronary-event rates to changes in coronary heart disease mortality: 10-year results from 37 WHO MONICA project populations. Monitoring trends and determinants in cardiovascular disease. Lancet 353, 15471557.
2Menotti, A, Kromhout, D, Blackburn, H, et al. (1999) Food intake patterns and 25-year mortality from coronary heart disease: cross-cultural correlations in the Seven Countries Study. The Seven Countries Study Research Group. Eur J Epidemiol 15, 507515.
3Keys, A, Menotti, A, Karvonen, MJ, et al. (1986) The diet and 15-year death rate in the seven countries study. Am J Epidemiol 124, 903915.
4Sofi, F, Cesari, F, Abbate, R, et al. (2008) Adherence to Mediterranean diet and health status: meta-analysis. BMJ 337, a1344.
5Buckland, G, Gonzalez, CA, Agudo, A, et al. (2009) Adherence to the Mediterranean diet and risk of coronary heart disease in the Spanish EPIC Cohort Study. Am J Epidemiol 170, 15181529.
6Martinez-Gonzalez, MA, Fernandez-Jarne, E, Serrano-Martinez, M, et al. (2002) Mediterranean diet and reduction in the risk of a first acute myocardial infarction: an operational healthy dietary score. Eur J Nutr 41, 153160.
7Estruch, R, Martinez-Gonzalez, MA, Corella, D, et al. (2006) Effects of a Mediterranean-style diet on cardiovascular risk factors: a randomized trial. Ann Intern Med 145, 111.
8Lopez-Miranda, J, Perez-Jimenez, F, Ros, E, et al. (2010) Olive oil and health: summary of the II international conference on olive oil and health consensus report, Jaen and Cordoba (Spain) 2008. Nutr Metab Cardiovasc Dis 20, 284294.
9Trichopoulou, A, Bamia, C, Norat, T, et al. (2007) Modified Mediterranean diet and survival after myocardial infarction: the EPIC-Elderly study. Eur J Epidemiol 22, 871881.
10Covas, MI, Konstantinidou, V & Fito, M (2009) Olive oil and cardiovascular health. J Cardiovasc Pharmacol 54, 477482.
11Ruiz-Canela, M & Martinez-Gonzalez, MA (2011) Olive oil in the primary prevention of cardiovascular disease. Maturitas 68, 245250.
12Barzi, F, Woodward, M, Marfisi, RM, et al. (2003) Mediterranean diet and all-causes mortality after myocardial infarction: results from the GISSI-Prevenzione trial. Eur J Clin Nutr 57, 604611.
13Trichopoulou, A, Bamia, C & Trichopoulos, D (2005) Mediterranean diet and survival among patients with coronary heart disease in Greece. Arch Intern Med 165, 929935.
14Fernandez-Jarne, E, Martinez-Losa, E, Prado-Santamaria, M, et al. (2002) Risk of first non-fatal myocardial infarction negatively associated with olive oil consumption: a case–control study in Spain. Int J Epidemiol 31, 474480.
15Kontogianni, MD, Panagiotakos, DB, Chrysohoou, C, et al. (2007) The impact of olive oil consumption pattern on the risk of acute coronary syndromes: The CARDIO2000 case–control study. Clin Cardiol 30, 125129.
16Bertuzzi, M, Tavani, A, Negri, E, et al. (2002) Olive oil consumption and risk of non-fatal myocardial infarction in Italy. Int J Epidemiol 31, 12741277.
17Bendinelli, B, Masala, G, Saieva, C, et al. (2011) Fruit, vegetables, and olive oil and risk of coronary heart disease in Italian women: the EPICOR Study. Am J Clin Nutr 93, 275283.
18Esposito, K, Marfella, R, Ciotola, M, et al. (2004) Effect of a Mediterranean-style diet on endothelial dysfunction and markers of vascular inflammation in the metabolic syndrome: a randomized trial. J Am Med Assoc 292, 14401446.
19Perez-Jimenez, F, Ruano, J, Perez-Martinez, P, et al. (2007) The influence of olive oil on human health: not a question of fat alone. Mol Nutr Food Res 51, 11991208.
20Visioli, F & Bernardini, E (2011) Extra virgin olive oil's polyphenols: biological activities. Curr Pharm Des 17, 786804.
21Martinez-Gonzalez, MA & Sanchez-Villegas, A (2004) The emerging role of Mediterranean diets in cardiovascular epidemiology: monounsaturated fats, olive oil, red wine or the whole pattern? Eur J Epidemiol 19, 913.
22Riboli, E, Hunt, KJ, Slimani, N, et al. (2002) European Prospective Investigation into Cancer and Nutrition (EPIC): study populations and data collection. Public Health Nutr 5, 11131124.
23Riboli, E & Kaaks, R (1997) The EPIC project: rationale and study design. Int J Epidemiol 26, S6S14.
24Danesh, J, Saracci, R, Berglund, G, et al. (2007) EPIC-Heart: the cardiovascular component of a prospective study of nutritional, lifestyle and biological factors in 520,000 middle-aged participants from 10 European countries. Eur J Epidemiol 22, 129141.
25EPIC Group of Spain (1997) Relative validity and reproducibility of a diet history questionnaire in Spain. I. Foods. EPIC Group of Spain. European Prospective Investigation into Cancer and Nutrition. Int J Epidemiol 26, Suppl. 1, S91S99.
26EPIC Group of Spain (1997) Relative validity and reproducibility of a diet history questionnaire in Spain. II. Nutrients. EPIC Group of Spain. European Prospective Investigation into Cancer and Nutrition. Int J Epidemiol 26, Suppl. 1, S100S109.
27Slimani, N, Deharveng, G, Unwin, I, et al. (2007) The EPIC nutrient database project (ENDB): a first attempt to standardize nutrient databases across the 10 European countries participating in the EPIC study. Eur J Clin Nutr 61, 10371056.
28Burke, B (1947) The dietary history as a tool in research. J Am Diet Assoc 23, 10411046.
29Luepker, RV, Apple, FS, Christenson, RH, et al. (2003) Case definitions for acute coronary heart disease in epidemiology and clinical research studies: a statement from the AHA Council on Epidemiology and Prevention; AHA Statistics Committee; World Heart Federation Council on Epidemiology and Prevention; the European Society of Cardiology Working Group on Epidemiology and Prevention; Centers for Disease Control and Prevention; and the National Heart, Lung, and Blood Institute. Circulation 108, 25432549.
30Willett, WC, Howe, GR & Kushi, LH (1997) Adjustment for total energy intake in epidemiologic studies. Am J Clin Nutr 65, 1220S1228S.
31Goldberg, GR, Black, AE, Jebb, SA, et al. (1991) Critical evaluation of energy intake data using fundamental principles of energy physiology: 1. Derivation of cut-off limits to identify under-recording. Eur J Clin Nutr 45, 569581.
32Tong, PC, Kong, AP, So, WY, et al. (2007) The usefulness of the International Diabetes Federation and the National Cholesterol Education Program's Adult Treatment Panel III definitions of the metabolic syndrome in predicting coronary heart disease in subjects with type 2 diabetes. Diabetes Care 30, 12061211.
33Salas-Salvado, J, Garcia-Arellano, A, Estruch, R, et al. (2008) Components of the Mediterranean-type food pattern and serum inflammatory markers among patients at high risk for cardiovascular disease. Eur J Clin Nutr 62, 651659.
34Carluccio, MA, Massaro, M, Scoditti, E, et al. (2007) Vasculo protective potential of olive oil components. Mol Nutr Food Res 51, 12251234.
35Razquin, C, Martinez, JA, Martinez-Gonzalez, MA, et al. (2009) A 3 years follow-up of a Mediterranean diet rich in virgin olive oil is associated with high plasma antioxidant capacity and reduced body weight gain. Eur J Clin Nutr 63, 13871393.
36Salas-Salvado, J, Bullo, M, Babio, N, et al. (2010) Reduction in the incidence of type 2-diabetes with the Mediterranean diet: results of the PREDIMED-Reus nutrition intervention randomized trial. Diabetes Care 34, 1419.
37Raederstorff, D (2009) Antioxidant activity of olive polyphenols in humans: a review. Int J Vitam Nutr Res 79, 152165.
38Covas, MI, Nyyssonen, K, Poulsen, HE, et al. (2006) The effect of polyphenols in olive oil on heart disease risk factors: a randomized trial. Ann Intern Med 145, 333341.
39Visioli, F, Bellomo, G & Galli, C (1998) Free radical-scavenging properties of olive oil polyphenols. Biochem Biophys Res Commun 247, 6064.
40Arriola, L, Martinez-Camblor, P, Larranaga, N, et al. (2010) Alcohol intake and the risk of coronary heart disease in the Spanish EPIC cohort study. Heart 96, 124130.
41Mendez, MA, Popkin, BM, Buckland, G, et al. (2011) Alternative methods of accounting for underreporting and overreporting when measuring dietary intake-obesity relations. Am J Epidemiol 173, 448458.
42Bingham, SA, Gill, C, Welch, A, et al. (1997) Validation of dietary assessment methods in the UK Arm of EPIC using weighed records, and 24-hour urinary nitrogen and potassium and serum vitamin C and carotenoids as biomarkers. Int J Epidemiol 26, S137S151.