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New Zealand green-lipped mussels (Perna canaliculus) enhance non-haem iron absorption in vitro

  • Robin J. C. Stewart (a1), Jane Coad (a1), Gordon W. Reynolds (a1) and Kevin C. Pedley (a1)

Abstract

Fe bioavailability can be manipulated by the nutritional composition of a meal. Ascorbic acid and unidentified components of meat, fish and poultry, but particularly beef, all appear to enhance the absorption of non-haem Fe. The aim of the present study is to identify whether extracts of green-lipped mussels (GLM; Perna canaliculus) enhance non-haem Fe absorption in Caco-2 cells and to compare the effect with that of beef. Raw GLM and raw beef homogenates were digested in vitro with pepsin at pH 2, and pancreatin and bile salts at pH 7. Tracer 55Fe was used to measure cellular Fe uptake. Ascorbic acid was used as a positive control and egg albumin, exposed to the same in vitro digestion process, was used as a negative control. Caco-2 cell monolayers were incubated with treatments for 60 min. All values were standardised per μg of GLM, egg albumin, beef or ascorbic acid. The results showed that ascorbic acid enhanced non-haem Fe absorption to the highest degree. Beef and GLM digestates both significantly enhanced Fe absorption compared with egg albumin. In conclusion, GLM digestate significantly enhances non-haem Fe uptake in Caco-2 cells with a similar magnitude to that of beef.

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Copyright

Corresponding author

*Corresponding author: Associate Professor K. C. Pedley, fax +64 6 350 5657, email k.c.pedley@massey.ac.nz

References

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1Cook, JD & Monsen, ER (1976) Food iron absorption in human subjects. III. Comparison of the effect of animal proteins on nonheme iron absorption. Am J Clin Nutr 29, 859867.
2Mulvihill, B, Kirwan, FM, Morrissey, PA, et al. (1998) Effect of myofibrillar muscle proteins on the in vitro bioavailability of non-haem iron. Int J Food Sci Nutr 49, 187.
3Huh, EC, Hotchkiss, A, Brouillette, J, et al. (2004) Carbohydrate fractions from cooked fish promote iron uptake by Caco-2 Cells. J Nutr 134, 16811689.
4Armah, CN, Sharp, P, Mellon, FA, et al. (2008) l-{alpha}-Glycerophosphocholine contributes to meat's enhancement of nonheme iron absorption. J Nutr 138, 873877.
5Laparra, JM, Tako, E, Glahn, RP, et al. (2008) Isolated glycosaminoglycans from cooked haddock enhance nonheme iron uptake by Caco-2 cells. J Agric Food Chem 56, 1034610351.
6Seiquer, I, Aspe, T, Perez-Granados, A, et al. (2002) Consumption of raw and fried sardine Clupea pilchardus as protein source of diets: effects on iron metabolism in rats. J Sci Food Agric 82, 14971503.
7Ohta, A, Ohtsuki, M, Baba, S, et al. (1995) Effects of fructooligosaccharides on the absorption of iron, calcium and magnesium in iron-deficient anemic rats. J Nutr Sci Vitaminol 41, 281.
8Cook, J & Monsen, E (1975) Food iron absorption. I. Use of semisynthetic diet to study absorption of nonheme iron. Am J Clin Nutr 28, 1289.
9Astrup, A, Dyerberg, J, Elwood, P, et al. (2011) The role of reducing intakes of saturated fat in the prevention of cardiovascular disease: where does the evidence stand in 2010? Am J Clin Nutr 93, 684688.
10Navas-Carretero, S, Perez-Granados, AM, Sarria, B, et al. (2008) Oily fish increases iron bioavailability of a phytate rich meal in young iron deficient women. J Am Coll Nutr 27, 96101.
11Rodriguez, M, Saiz, M, Muntane, J, et al. (1996) Fatty acid composition of erythrocyte membranes affects iron absorption in rats. J Nutr 126, 3109.
12Murphy, K, Mann, N & Sinclair, A (2003) Fatty acid and sterol composition of frozen and freeze-dried New Zealand green lipped mussel (Perna canaliculus) from three sites in New Zealand. Asia Pac J Clin Nutr 12, 50.
13Ahn, D, Wolfe, F & Sim, J (1993) Three methods for determining nonheme iron in turkey meat. J Food Sci 58, 288291.
14Teucher, B, Olivares, M & Cori, H (2004) Enhancers of iron absorption: ascorbic acid and other organic acids. Int J Vitam Nutr Res 74, 403420.
15Garcia, MN, Flowers, C & Cook, JD (1996) The Caco-2 cell culture system can be used as a model to study food iron availability. J Nutr 126, 251258.
16Kojima, N, Wallace, D & Bates, G (1981) The effect of chemical agents, beverages, and spinach on the in vitro solubilization of iron from cooked pinto beans. Am J Clin Nutr 34, 1392.
17Conrad, M & Schade, S (1968) Ascorbic acid chelates in iron absorption: a role for hydrochloric acid and bile. Gastroenterology 55, 35.
18Kapsokefalou, M & Miller, D (1993) Lean beef and beef fat interact to enhance nonheme iron absorption in rats. J Nutr 123, 1429.
19Hurrell, RF, Reddy, MB, Juillerat, M, et al. (2006) Meat protein fractions enhance nonheme iron absorption in humans. J Nutr 136, 28082812.
20Glahn, R, Wien, E, van Campen, D, et al. (1996) Caco-2 cell iron uptake from meat and casein digests parallels in vivo studies: use of a novel in vitro method for rapid estimation of iron bioavailability. J Nutr 126, 332.
21Kier, WM (1988) Arrangement and function of molluscan muscle. In The Mollusca [Wilbur, KM, editor]. Durham, NC: Academic Press, Inc.
22McPhee, S, Hodges, L, Wright, P, et al. (2007) Anti-cyclooxygenase effects of lipid extracts from the New Zealand green-lipped mussel, Perna canaliculus. Comp Biochem Physiol Part B: Biochem Mol Biol 146, 346356.
23Bui, L & Bierer, T (2003) Influence of green lipped mussels (Perna canaliculus) in alleviating signs of arthritis in dogs. Vet Ther 4, 397407.

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