Skip to main content Accessibility help
×
Home

A monounsaturated fatty acid-rich diet reduces macrophage uptake of plasma oxidised low-density lipoprotein in healthy young men

  • Juan Antonio Moreno (a1) (a2), José López-Miranda (a1) (a2), Pablo Pérez-Martínez (a1) (a2), Carmen Marín (a1) (a2), Rafael Moreno (a1) (a2), Purificación Gómez (a1) (a2), Juan Antonio Paniagua (a1) (a2) and Francisco Pérez-Jiménez (a1) (a2)...

Abstract

During atherogenesis, a pathological accumulation of lipids occurs within aortic intimal macrophages through uptake of plasma oxidised LDL (oxLDL). The aim of the present study was to determine whether macrophage uptake of plasma oxLDL and LDL susceptibility to oxidation may be determined by quantity and quality of dietary fat. Twenty healthy young men were subjected to three dietary periods, each lasting 4 weeks. The first was an SFA-enriched diet (38 % fat, 20 % SFA), which was followed by a carbohydrate (CHO)-rich diet (30 % fat, < 10 % SFA, 55 % CHO) or a MUFA olive oil-rich diet (38 % fat, 22 % MUFA) following a randomised cross-over design. After each diet period, LDL particles were oxidised with Cu ions to determine LDL susceptibility to oxidation and subsequently incubated with the U937-macrophage cell line to determine the percentage of uptake of plasma oxLDL. The shift from the MUFA diet to the SFA- or CHO-rich diets reduced the resistance of LDL particles to oxidation, decreasing lag time (P = 0·038) and increasing the propagation rate (P = 0·001). Furthermore, the MUFA-rich diet demonstrated reduced macrophage uptake of plasma oxLDL (P = 0·031) as compared with the SFA-rich diet. Finally, macrophage uptake of plasma oxLDL was correlated (r 0·45; P = 0·040) with total amount of conjugated dienes after LDL oxidation. Our data suggest that a MUFA-rich diet may have favourable effects on cardiovascular risk since it prevents the oxidative modifications of LDL and reduces macrophage uptake of plasma oxLDL.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      A monounsaturated fatty acid-rich diet reduces macrophage uptake of plasma oxidised low-density lipoprotein in healthy young men
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      A monounsaturated fatty acid-rich diet reduces macrophage uptake of plasma oxidised low-density lipoprotein in healthy young men
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      A monounsaturated fatty acid-rich diet reduces macrophage uptake of plasma oxidised low-density lipoprotein in healthy young men
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: Dr José López-Miranda, fax +34 957 218250, email md1lomij@uco.es

References

Hide All
1 Keys, A, Menotti, A, Karvonen, MJ, et al. (1986) The diet and 15-year death rate in the seven countries study. Am J Epidemiol 124, 903915.
2 Trichopoulou, A, Kouris-Blazos, A, Vassilakou, T, Gnardellis, C, Polychronopoulos, E, Venizelos, M, Lagiou, P, Wahlqvist, ML & Trichopoulos, D (1995) Diet and survival of elderly Greeks: a link to the past. Am J Clin Nutr 61, 1346S1350S.
3 Castelli, WP, Garrison, RJ, Wilson, PW, Abbott, RD, Kalousdian, S & Kannel, WB (1986) Incidence of coronary heart disease and lipoprotein cholesterol levels. The Framingham Study. JAMA 256, 28352838.
4 Mensink, RP & Katan, MB (1992) Effect of dietary fatty acids on serum lipids and lipoproteins. A meta-analysis of 27 trials. Arterioscler Thromb 12, 911919.
5 Grundy, SM, Nix, D, Whelan, MF & Franklin, L (1986) Comparison of three cholesterol-lowering diets in normolipidemic men. JAMA 256, 23512355.
6 Perez-Jimenez, F, Lopez-Miranda, J & Mata, P (2002) Protective effect of dietary monounsaturated fat on arteriosclerosis: beyond cholesterol. Atherosclerosis 163, 385398.
7 Perez-Jimenez, F (2005) International conference on the healthy effect of virgin olive oil. Eur J Clin Invest 35, 421424.
8 Witztum, JL (1994) The role of oxidized LDL in the atherogenic process. J Atheroscler Thromb 1, 7175.
9 Gerrity, RG (1981) The role of the monocyte in atherogenesis: II. Migration of foam cells from atherosclerotic lesions. Am J Pathol 103, 191200.
10 Gerrity, RG (1981) The role of the monocyte in atherogenesis: I. Transition of blood-borne monocytes into foam cells in fatty lesions. Am J Pathol 103, 181190.
11 Mortensen, A, Espensen, PL, Hansen, BF & Ibsen, P (1992) The influence of dietary olive oil and margarine on aortic cholesterol accumulation in cholesterol-fed rabbits maintained at similar plasma cholesterol level. Atherosclerosis 96, 159170.
12 Krieger, M & Herz, J (1994) Structures and functions of multiligand lipoprotein receptors: macrophage scavenger receptors and LDL receptor-related protein (LRP). Annu Rev Biochem 63, 601637.
13 Miles, EA, Wallace, FA & Calder, PC (2001) An olive oil-rich diet reduces scavenger receptor mRNA in murine macrophages. Br J Nutr 85, 185191.
14 Parthasarathy, S, Khoo, JC, Miller, E, Barnett, J, Witztum, JL & Steinberg, D (1990) Low density lipoprotein rich in oleic acid is protected against oxidative modification: implications for dietary prevention of atherosclerosis. Proc Natl Acad Sci U S A 87, 38943898.
15 Aviram, M & Eias, K (1993) Dietary olive oil reduces low-density lipoprotein uptake by macrophages and decreases the susceptibility of the lipoprotein to undergo lipid peroxidation. Ann Nutr Metab 37, 7584.
16 Reaven, P, Parthasarathy, S, Grasse, BJ, Miller, E, Almazan, F, Mattson, FH, Khoo, JC, Steinberg, D & Witztum, JL (1991) Feasibility of using an oleate-rich diet to reduce the susceptibility of low-density lipoprotein to oxidative modification in humans. Am J Clin Nutr 54, 701706.
17 Ramirez-Tortosa, C, Lopez-Pedrosa, JM, Suarez, A, Ros, E, Mataix, J & Gil, A (1999) Olive oil- and fish oil-enriched diets modify plasma lipids and susceptibility of LDL to oxidative modification in free-living male patients with peripheral vascular disease: the Spanish Nutrition Study. Br J Nutr 82, 3139.
18 Human Nutrition Information Service (1987) Agriculture Handbook No. 8. Washington, DC: US Government Printing Office.
19 Varela, G (1980) Tablas de Composición de Alimentos (Food Composition Tables). Madrid: Instituto de Nutrición CSIC.
20 Association of Official Analytical Chemists (1990) Official Methods of Analysis, 15th ed. Arlington, VA: Association of Official Analytical Chemists.
21 Ruiz-Gutierrez, V, Prada, JL & Perez-Jimenez, F (1993) Determination of fatty acid and triacylglycerol composition of human very-low-density lipoproteins. J Chromatogr 622, 117124.
22 Allan, CM, Taylor, S & Taylor, JM (1997) Two hepatic enhancers, HCR.1 and HCR.2, coordinate the liver expression of the entire human apolipoprotein E/C-I/C-IV/C-II gene cluster. J Biol Chem 272, 2911329119.
23 Bucolo, G & David, H (1973) Quantitative determination of serum triglycerides by the use of enzymes. Clin Chem 19, 476482.
24 Assmann, G, Schriewer, H, Schmitz, G & Hagele, EO (1983) Quantification of high-density-lipoprotein cholesterol by precipitation with phosphotungstic acid/MgCl2. Clin Chem 29, 20262030.
25 Riepponen, P, Marniemi, J & Rautaoja, T (1987) Immunoturbidimetric determination of apolipoproteins A-I and B in serum. Scand J Clin Lab Invest 47, 739744.
26 Friedewald, WT, Levy, RI & Fredrickson, DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18, 499502.
27 Bradford, MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248254.
28 Esterbauer, H, Striegl, G, Puhl, H & Rotheneder, M (1989) Continuous monitoring of in vitro oxidation of human low density lipoprotein. Free Radic Res Commun 6, 6775.
29 Okada, Y, Kimura, T, Kameoka, M, Kishi, M, Azuma, I & Ikuta, K (1995) Viral activation from latency during retrodifferentiation of U937 cells exposed to phorbol ester followed by infection with human immunodeficiency virus type 1. Immunopharmacology 30, 2739.
30 Moreno, JA, Perez-Jimenez, F, Marin, C, Gomez, P, Perez-Martinez, P, Moreno, R, Bellido, C, Fuentes, F & Lopez-Miranda, J (2004) The effect of dietary fat on LDL size is influenced by apolipoprotein E genotype in healthy subjects. J Nutr 134, 25172522.
31 Connor, WE & Connor, SL (1997) Should a low-fat, high-carbohydrate diet be recommended for everyone? The case for a low-fat, high-carbohydrate diet. N Engl J Med 337, 562563, discussion 566–567.
32 Witztum, JL & Steinberg, D (1991) Role of oxidized low density lipoprotein in atherogenesis. J Clin Invest 88, 17851792.
33 Visioli, F, Bellomo, G, Montedoro, G & Galli, C (1995) Low density lipoprotein oxidation is inhibited in vitro by olive oil constituents. Atherosclerosis 117, 2532.
34 Moreno, JA, Perez-Jimenez, F, Marin, C, Gomez, P, Perez-Martinez, P, Moreno, R, Bellido, C, Fuentes, F & Lopez-Miranda, J (2004) Apolipoprotein E gene promoter − 219G → T polymorphism increases LDL-cholesterol concentrations and susceptibility to oxidation in response to a diet rich in saturated fat. Am J Clin Nutr 80, 14041409.
35 Moreno, JA, Lopez-Miranda, J, Gomez, P, Benkhalti, F, El Boustani, ES & Perez-Jimenez, F (2003) Effect of phenolic compounds of virgin olive oil on LDL oxidation resistance (article in Spanish). Med Clin (Barc) 120, 128131.
36 Visioli, F & Galli, C (2001) Antiatherogenic components of olive oil. Curr Atheroscler Rep 3, 6467.
37 Ruano, J, Lopez-Miranda, J, Fuentes, F, Moreno, JA, Bellido, C, Perez-Martinez, P, Lozano, A, Gomez, P, Jimenez, Y & Perez Jimenez, F (2005) Phenolic content of virgin olive oil improves ischemic reactive hyperemia in hypercholesterolemic patients. J Am Coll Cardiol 46, 18641868.
38 Sakaguchi, H, Takeya, M, Suzuki, H, et al. (1998) Role of macrophage scavenger receptors in diet-induced atherosclerosis mice. Lab Invest 78, 423434.
39 Yla-Herttuala, S, Rosenfeld, ME, Parthasarathy, S, Sigal, E, Sarkioja, T, Witztum, JL & Steinberg, D (1991) Gene expression in macrophage-rich human atherosclerotic lesions. 15-Lipoxygenase and acetyl low density lipoprotein receptor messenger RNA colocalize with oxidation specific lipid-protein adducts. J Clin Invest 87, 11461152.
40 Nagy, L, Tontonoz, P, Alvarez, JG, Chen, H & Evans, RM (1998) Oxidized LDL regulates macrophage gene expression through ligand activation of PPARγ. Cell 93, 229240.
41 Han, J & Nicholson, AC (1998) Lipoproteins modulate expression of the macrophage scavenger receptor. Am J Pathol 152, 16471654.
42 Yoshida, H, Quehenberger, O, Kondratenko, N, Green, S & Steinberg, D (1998) Minimally oxidized low-density lipoprotein increases expression of scavenger receptor A, CD36, and macrosialin in resident mouse peritoneal macrophages. Arterioscler Thromb Vasc Biol 18, 794802.

Keywords

A monounsaturated fatty acid-rich diet reduces macrophage uptake of plasma oxidised low-density lipoprotein in healthy young men

  • Juan Antonio Moreno (a1) (a2), José López-Miranda (a1) (a2), Pablo Pérez-Martínez (a1) (a2), Carmen Marín (a1) (a2), Rafael Moreno (a1) (a2), Purificación Gómez (a1) (a2), Juan Antonio Paniagua (a1) (a2) and Francisco Pérez-Jiménez (a1) (a2)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed