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Molecular hydrogen generated by elemental magnesium supplementation alters rumen fermentation and microbiota in goats

  • Min Wang (a1) (a2), Rong Wang (a1) (a3), XiuMin Zhang (a1), Emilio M. Ungerfeld (a4), Donglei Long (a1) (a3), HongXiang Mao (a1) (a3), JinZhen Jiao (a1), Karen A. Beauchemin (a5) and Zhiliang Tan (a1) (a2)...

Abstract

We tested the hypotheses that supplementation of a diet with elemental Mg increases ruminal dissolved H2 (dH2) in rumen fluid, which in turn alters rumen fermentation and microbial community in goats. In a randomised block design, twenty growing goats were allocated to two treatments fed the same basal diet with 1·45 % Mg(OH)2 or 0·6 % elemental Mg. After 28 d of adaptation, we collected total faeces to measure total tract digestibility, rumen contents to analyse fermentation end products and microbial groups, and measured methane (CH4) emission using respiration chambers. Ruminal Mg2+ concentration was similar in both treatments. Elemental Mg supplementation increased dH2 at 2·5 h post morning feeding (+180 %, P<0·001). Elemental Mg supplementation decreased total volatile fatty acid concentration (−8·6 %, P<0·001), the acetate:propionate ratio (−11·8 %, P<0·03) and fungal copy numbers (−63·6 %, P=0·006), and increased propionate molar percentage (+11·6 %, P<0·001), methanogen copy numbers (+47·9 %, P<0·001), dissolved CH4 (+35·6 %, P<0·001) and CH4 emissions (+11·7 %, P=0·03), compared with Mg(OH)2 supplementation. The bacterial community composition in both treatments was overall similar. Ruminal dH2 was negatively correlated with acetate molar percentage and fungal copy numbers (P<0·05), and positively correlated with propionate molar percentage and methanogen copy numbers (P<0·05). In summary, elemental Mg supplementation increased ruminal dH2 concentration, which inhibited rumen fermentation, enhanced methanogenesis and seemed to shift fermentation pathways from acetate to propionate, and altered microbiota by decreasing fungi and increasing methanogens.

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Corresponding author

* Corresponding author: Z. Tan, fax+86 731 4612685, email zltan@isa.ac.cn

References

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1. Vyas, D, McGinn, SM, Duval, SM, et al. (2016) Effects of sustained reduction of enteric methane emissions with dietary supplementation of 3-nitrooxypropanol on growth performance of growing and finishing beef cattle1. J Anim Sci 94, 20242034.
2. Janssen, PH (2010) Influence of hydrogen on rumen methane formation and fermentation balances through microbial growth kinetics and fermentation thermodynamics. Anim Feed Sci Technol 160, 122.
3. Joblin, KN (1999) Ruminal acetogens and their potential to lower ruminant methane emissions. Aust J Agric Res 50, 13071313.
4. Ungerfeld, EM & Kohn, RA (2006) The role of thermodynamics in control of ruminal fermentation. In Ruminant Physiology Digestion, Metabolism and Impact of Nutrition on Gene Expression, Immunology and Stress, pp. 5585 [K Sejrsen, T Hvelpund and MO Nielsen, editors]. Wageningen: Wageningen Academic Publishers.
5. Wang, M, Wang, R, Xie, T, et al. (2016) Shifts in rumen fermentation and microbiota are associated with dissolved ruminal hydrogen concentrations in lactating dairy cows fed different types of carbohydrates. J Nutr 146, 17141721.
6. Martinez-Fernandez, G, Denman, SE, Yang, C, et al. (2016) Methane inhibition alters the microbial community, hydrogen flow, and fermentation response in the rumen of cattle. Front Microbiol 7, 1122.
7. Mitsumori, M, Shinkai, T, Takenaka, A, et al. (2012) Responses in digestion, rumen fermentation and microbial populations to inhibition of methane formation by a halogenated methane analogue. Br J Nutr 108, 482491.
8. Patra, AK & Yu, Z (2013) Effects of gas composition in headspace and bicarbonate concentrations in media on gas and methane production, degradability, and rumen fermentation using in vitro gas production techniques. J Dairy Sci 96, 45924600.
9. Klop, G, Dijkstra, J, Dieho, K, et al. (2017) Enteric methane production in lactating dairy cows with continuous feeding of essential oils or rotational feeding of essential oils and lauric acid. J Dairy Sci 100, 35633575.
10. Qiao, JY, Tan, ZL, Guan, LL, et al. (2015) Effects of hydrogen in headspace and bicarbonate in media on rumen fermentation, methane production and methanogenic population using in vitro gas production techniques. Anim Feed Sci Technol 206, 1928.
11. Olijhoek, DW, Hellwing, ALF, Weisbjerg, MR, et al. (2016) Effect of short-term infusion of hydrogen on enteric gas production and rumen environment in dairy cows. Anim Prod Sci 56, 466471.
12. Broudiscou, LP, Offner, A & Sauvant, D (2014) Effects of inoculum source, pH, redox potential and headspace di-hydrogen on rumen in vitro fermentation yields. Animal 8, 931937.
13. Wang, M, Sun, XZ, Janssen, PH, et al. (2014) Responses of methane production and fermentation pathways to the increased dissolved hydrogen concentration generated by eight substrates in in vitro ruminal cultures. Anim Feed Sci Technol 194, 111.
14. Wang, M, Ungerfeld, EM, Wang, R, et al. (2016) Supersaturation of dissolved hydrogen and methane in rumen of Tibetan sheep. Front Microbiol 7, 850.
15. Ng, F, Kittelmann, S, Patchett, ML, et al. (2016) An adhesin from hydrogen-utilizing rumen methanogen Methanobrevibacter ruminantium M1 binds a broad range of hydrogen-producing microorganisms. Environ Microbiol 18, 30103021.
16. Wang, M, Wang, R, Janssen, PH, et al. (2016) Sampling procedure for the measurement of dissolved hydrogen and volatile fatty acids in the rumen of dairy cows. J Anim Sci 94, 11591169.
17. Wang, M, Wang, R, Sun, X, et al. (2015) A mathematical model to describe the diurnal pattern of enteric methane emissions from non-lactating dairy cows post-feeding. Anim Nutr 1, 329338.
18. McGinn, SM, Beauchemin, KA, Coates, T, et al. (2004) Methane emissions from beef cattle: effects of monensin, sunflower oil, enzymes, yeast, and fumaric acid. J Anim Sci 82, 33463356.
19. Association of Official Analytical Chemists (editor) (1995) Official Methods of Analysis, 16th ed. Arlington, VA: AOAC.
20. Van Soest, PJ, Robertson, JB & Lewis, BA (1991) Symposium: carbohydrate methodology, metabolism and nutritional implications in dairy cattle. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74, 35833597.
21. Kartchner, RJ & Theurer, B (1981) Comparison of hydrolysis methods used in feed, digesta, and fecal starch analysis. J Agri Food Chem 29, 811.
22. Weatherburn, MW (1967) Phenol-hypochlorite reaction for determination of ammonia. Anal Chem 39, 971974.
23. Nelson, N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153, 375380.
24. Hille, KT, Hetz, SK, Rosendahl, J, et al. (2016) Determination of Henry’s constant,, the dissociation constant, and the buffer capacity of the bicarbonate system in ruminal fluid. J Dairy Sci 99, 369385.
25. Carroll, JJ, Slupsky, JD & Mather, AE (1991) The solubility of carbon-dioxide in water at low-pressure. J Phys Chem Ref Data 20, 12011209.
26. Guyader, J, Eugène, M, Doreau, M, et al. (2017) Tea saponin reduced methanogenesis in vitro but increased methane yield in lactating dairy cows. J Dairy Sci 100, 18451855.
27. Jiao, J, Lu, Q, Tan, Z, et al. (2014) In vitro evaluation of effects of gut region and fiber structure on the intestinal dominant bacterial diversity and functional bacterial species. Anaerobe 28, 168177.
28. Stevenson, D & Weimer, P (2007) Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR. Appl Microbiol Biotechnol 75, 165174.
29. Hackmann, TJ & Firkins, JL (2015) Electron transport phosphorylation in rumen butyrivibrios: unprecedented ATP yield for glucose fermentation to butyrate. Front Microbiol 6, 622.
30. Edgar, RC (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26, 24602461.
31. DeSantis, TZ, Hugenholtz, P, Larsen, N, et al. (2006) Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Appl Environ Microbiol 72, 50695072.
32. Ungerfeld, EM (2015) Shifts in metabolic hydrogen sinks in the methanogenesis-inhibited ruminal fermentation: a meta-analysis. Front Microbiol 6, 37.
33. Hristov, AN, Oh, J, Giallongo, F, et al. (2015) An inhibitor persistently decreased enteric methane emission from dairy cows with no negative effect on milk production. PNAS 112, 1066310668.
34. Romero-Perez, A, Okine, EK, McGinn, SM, et al. (2015) Sustained reduction in methane production from long-term addition of 3-nitrooxypropanol to a beef cattle diet. J Anim Sci 93, 17801791.
35. Bannink, A, Kogut, J, Dijkstra, J, et al. (2006) Estimation of the stoichiometry of volatile fatty acid production in the rumen of lactating cows. J Theol Biol 238, 3651.
36. Shinkai, T, Enishi, O, Mitsumori, M, et al. (2012) Mitigation of methane production from cattle by feeding cashew nut shell liquid. J Dairy Sci 95, 53085316.
37. Stewart, CS, Flint, HJ & Bryant, MP (1997) The rumen bacteria. In The Rumen Microbial Ecosystem, pp. 1072 [PN Hobson and CS Stewart, editors]. London: Blackie Academic & Professional.
38. Philippeau, C, Lettat, A, Martin, C, et al. (2017) Effects of bacterial direct-fed microbials on ruminal characteristics, methane emission, and milk fatty acid composition in cows fed high- or low-starch diets. J Dairy Sci 100, 26372650.
39. Fonty, G, Joblin, K, Chavarot, M, et al. (2007) Establishment and development of ruminal hydrogenotrophs in methanogen-free lambs. Appl Environ Microbiol 73, 63916403.
40. Scheifinger, CC, Linehan, B & Wolin, MJ (1975) H2 production by Selenomonas ruminantium in the absence and presence of methanogenic bacteria. Appl Microbiol 29, 480483.
41. Sawanon, S, Koike, S & Kobayashi, Y (2011) Evidence for the possible involvement of Selenomonas ruminantium in rumen fiber digestion. FEMS Microbiol Lett 325, 170179.
42. McAllister, TA & Newbold, CJ (2008) Redirecting rumen fermentation to reduce methanogenesis. Aust J Exp Agric 48, 713.

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