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Mixed function oxidases in response to different types of dietary lipids in rats

Published online by Cambridge University Press:  09 March 2007

Morio Saito ,
Akira Oh-Hashi ,
Mika Kubota ,
Eiichi Nishide  and
Michio Yamaguchi
Morio Saito
Affiliation:
Division of Food Science, National Institute of Health and Nutrition, 1-23-1, Toyama, Shinjuku-ku, Tokyo 162, Japan
Akira Oh-Hashi
Affiliation:
Division of Food Science, National Institute of Health and Nutrition, 1-23-1, Toyama, Shinjuku-ku, Tokyo 162, Japan
Mika Kubota
Affiliation:
Division of Food Science, National Institute of Health and Nutrition, 1-23-1, Toyama, Shinjuku-ku, Tokyo 162, Japan
Eiichi Nishide
Affiliation:
Department of Fisheries, College of Agriculture and Veterinary Medicine, Nihon University, Tokyo 154, Japan
Michio Yamaguchi
Affiliation:
Division of Food Science, National Institute of Health and Nutrition, 1-23-1, Toyama, Shinjuku-ku, Tokyo 162, Japan
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Abstract

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The influence of dietary lipids on the liver microsomal mixed function oxidase system and on pentobarbital-induced sleeping time was studied in rats. Giving diets containing (g/kg) 150 olive oil, 150 lard or 150 soya-bean oil for 21 d (Expt 1) increased the cytochrome P-450 content in the order: olive oil < lard < soya-bean oil. When diets containing (g/kg) 150 lard, 150 soya-bean oil, 150 sardine oil or an equal mixture of 50 of each oil were given for 15 d (Expt 2), the cytochrome P-450 content and aminopyrine N-demethylase activity were significantly higher in the sardine-oil and mixed-oil groups than in the lard group, and the activity of aminopyrine N-demethylase was also significantly higher in the soya-bean oil group compared with the lard group. A significantly higher activity of NADPH-cytochrome c reductase (EC 1.6.2.5) was observed in the sardine-oil group than in the other three groups. Aniline hydroxylase activity and cytochrome b5 content remained unchanged in all the groups. Pentobarbital-induced sleeping time measured on day 15 (Expt 2) varied inversely with the changes in cytochrome P-450 content and aminopyrine N-demethylase activity in the three single-fat groups, but not in the mixed-oil group, reflecting liver microsomal metabolic activity for pentobarbital in vivo. From these results, it appears that high intakes of polyunsaturated fatty acids (18:2n-6, 18:3n-3, 20:5n-3 and 22:6n-3) stimulate the liver microsomal mixed function oxidase system.

Keywords

Dietary lipidsMixed function oxidaseSleeping timeRat
Type
Dietary lipids and Metabolism
Information
British Journal of Nutrition , Volume 63 , Issue 2 , March 1990 , pp. 249 - 257
Copyright
Copyright © The Nutrition Society 1990

References

Anderson, K. E., Conney, A. H. & Kappas, A. (1982). Nutritional influences on chemical biotransformations in humans. Nutrition Reviews 40, 161–171.CrossRefGoogle ScholarPubMed
Bieri, J. G., Stoewsand, G. S., Briggs, G. M., Phillips, R. W., Woodard, J. C. & Knapka, J. J. (1977). Report of the American Institute of Nutrition Ad Hoc Committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.Google Scholar
Century, B. (1973). A role of the dietary lipid in the ability of phenobarbital to stimulate drug detoxification. Journal of Pharmacology and Experimental Therapeutics 185, 185–194.Google ScholarPubMed
Clinton, S. K., Mulloy, A. L. & Visek, W. J. (1984). Effects of dietary lipid saturation on prolactin secretion, carcinogen metabolism and mammary carcinogenesis in rats. Journal of Nutrition 114, 1630–1639.CrossRefGoogle ScholarPubMed
Duncan, D. B. (1957). Multiple range tests for correlated and heteroscedastic means. Biometrics 13, 164–176.CrossRefGoogle Scholar
Hammer, C. T. & Wills, E. D. (1979). The effect of dietary fats on the composition of the liver endoplasmic reticulum and oxidative drug metabolism. British Journal of Nutrition 41, 465–475.CrossRefGoogle Scholar
Hopkins, G. J. & West, C. E. (1976). Effect of dietary fats on pentobarbitone-induced sleeping times and hepatic microsomal cytochrome P-450 in rats. Lipids 11, 736–740.CrossRefGoogle ScholarPubMed
Imai, Y., Ito, A. & Sato, R. (1966). Evidence for biochemically different types of vesicles in the hepatic microsomal fraction. Journal of Biochemistry, Tokyo 60, 417–428.CrossRefGoogle ScholarPubMed
Kaschnitz, R. (1970). Aryl 4-hydroxylase, cytochrome P-450 and microsomal lipids in essential fatty acid deficiency. Hoppe-Seyler's Zeitschrift fur Physiologische Chemie 351, 771–774.Google ScholarPubMed
Laitinen, M., Hietanen, E., Vainio, H. & Hanninen, O. (1975). Dietary fats and properties of endoplasmic reticulum. I. Dietary lipid induced changes in composition of microsomal membranes in liver and gastroduodenal mucosa of rat. Lipids 10, 461–466.CrossRefGoogle ScholarPubMed
Lambert, L. & Wills, E. D. (1977). The effect of dietary lipid peroxides, sterols and oxidised sterols on cytochrome P450 and oxidative demethylation in the endoplasmic reticulum. Biochemical Pharmacology 26, 1417–1421.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Lu, A. Y. H. (1976). Liver microsomal drug-metabolizing enzyme system: functional components and their properties. Federation Proceedings 35, 2460–2463.Google ScholarPubMed
Marshall, W. J. & McLean, A. E. M. (1971a). Dietary lipid requirements for hepatic microsomal enzyme induction in the rat. Proceedings of the Nutrition Society 30, 66A–67A.Google Scholar
Marshall, W. J. & McLean, A. E. M. (1971b). A requirement for dietary lipids for induction of cytochrome P-450 by phenobarbitone in rat liver microsomal fraction. Biochemical Journal 122, 569–573.CrossRefGoogle ScholarPubMed
Meydani, M., Blumberg, J. B. & Hayes, K. C. (1985). Dietary fat unsaturation enhances drug metabolism in Cebus but not in Squirrel monkeys. Journal of Nutrition 115, 573–578.CrossRefGoogle ScholarPubMed
Miller, O. N. (1976). Nutrition and drug metabolism. Introduction. Federation Proceedings 35, 2459.Google Scholar
Mino, M., Tamai, H., Yasuda, K., Yamada, C., Igarashi, O., Hayashi, M., Hirahara, F., Katsui, G. & Kijima, S. (1988). Biopotencies of tocopherol analogues as determined by diarulic acid induced hemolysis in rats. Vitamins (Japan) 62, 241–246.Google Scholar
Nash, T. (1953). The colorimetric estimation of formaldehyde by means of the Hantzsch reaction. Biochemical Journal 55, 416–421.CrossRefGoogle ScholarPubMed
Norred, W. P. & Wade, A. E. (1972). Dietary fatty acid-induced alterations of hepatic microsomal drug metabolism. Biochemical Pharmacology 21, 2887–2897.CrossRefGoogle ScholarPubMed
Omura, T. & Sato, R. (1964). The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. Journal of Biological Chemistry 239, 2370–2378.CrossRefGoogle ScholarPubMed
Omura, T. & Takesue, S. (1970). A new method for simultaneous purification of cytochrome b5 and NADPH- cytochrome c reductase from rat liver microsomes. Journal of Biochemistry 67, 249–258.CrossRefGoogle ScholarPubMed
Rowe, L. & Wills, E. D. (1976). The effect of dietary lipids and vitamin E on lipid peroxide formation, cytochrome P-450 and oxidative demethylation in the endoplasmic reticulum. Biochemical Pharmacology 25, 175–179.CrossRefGoogle ScholarPubMed
Saito, M. & Yamaguchi, M. (1988). Influence of excessive ascorbic acid dose on liver microsomal mixed function oxidase system in guinea pigs. Journal of Clinical Biochemistry and Nutrition 4, 123–137.CrossRefGoogle Scholar
Wade, A. E. & Norred, W. P. (1976). Effect of dietary lipid on drug-metabolizing enzymes. Federation Proceedings 35, 2475–2479.Google ScholarPubMed
Wade, A. E., Wu, B. & Caster, W. O. (1972). Relationship of dietary essential fatty acid consumption to hepatic drug hydroxylation. Pharmacology 7, 305–314.CrossRefGoogle ScholarPubMed
Zannoni, V. G. & Sato, P. H. (1976). The effect of certain vitamin deficiencies on hepatic drug metabolism. Federation Proceedings 35, 2464–2469.Google ScholarPubMed