Skip to main content Accessibility help
×
Home

Mineral excretion of rats fed on diets containing faba beans (Vicia faba L.) or faba bean fractions

  • Luis A. Rubio (a1), George Grant (a1), Susan Bardocz (a1), Peter Dewey (a1) and A. Pusztai (a1)...

Abstract

The effects on faecal mineral excretion of two commercial varieties (local cultivar and Troy cultivar) of raw faba beans (Vicia faba L., minor) meal (VFM) and its fractions have been studied in growing rats. Diets contained local-VFM (dark seed coat) and Troy-VFM (light seed coat) at 474–500 g/kg diet, hull (VFH) from both varieties at 65 g/kg diet, and the insoluble cotyledon residue (VFCR) obtained from the Troy variety at 237 g/kg diet. Rats were pair-fed on diets which had been supplemented with amino acids to target requirements and contained similar amounts of zinc, manganese, iron and copper. With VFM diets the apparent absorption of Zn and Mn was significantly reduced. On the other hand, with hulls the apparent absorption of Fe was reduced while that of Cu slightly increased. As the amounts of calcium, phosphorus and magnesium in VFM and VFH diets were higher than in the controls, the increased intake resulted in a significant increase in both the apparent absorption and the faecal excretion of these minerals. The inclusion of VFCR in the diet had no significant effect on the mineral content of faeces. The relatively low concentrations of phytate in the bean seeds of 7.8 and 6.7 g/kg for the local and Troy cultivars respectively, could not adequately account for the increased mineral excretion. The results suggest that other seed constituents, possibly the soluble non-starch polysaccharides, may be involved in the elevated loss of Zn and Mn in rats fed on diets containing faba bean for extended periods, while some insoluble structural hull components may interfere with the absorption of Fe from the gut.

Faba bean (Vicia faba): Faecal mineral excretion: Rat.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Mineral excretion of rats fed on diets containing faba beans (Vicia faba L.) or faba bean fractions
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Mineral excretion of rats fed on diets containing faba beans (Vicia faba L.) or faba bean fractions
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Mineral excretion of rats fed on diets containing faba beans (Vicia faba L.) or faba bean fractions
      Available formats
      ×

Copyright

References

Hide All
Anderson, J. W. & Chen, W.-J. L. (1979) Plant fibre, carbohydrate and lipid metabolism. American Journal of Clinical Nutrition 32, 346363.
Anderson, J. W., Story, L., Sieling, B., Chen, W.-J. L., Petro, M. S. & Story, J. (1984) Hypocholesterolemic effects of oat-bran or bean intake for hypercholesterolemic men. American Journal of Clinical Nutrition 40, 11461155.
Coates, M. E., O'Donoghue, P. N., Payne, P. R. & Ward, R. J. (1969) In Laboratory Animal Handbooks. 2. Laboratory Standards for Laboratory Rats and Mice, p. 15. London: Laboratory Animals Ltd.
Davies, N. T., Carswell, A. J. P. & Mills, C. F. (1985) The effect of variation in dietary calcium intake on the phytate-Zn interaction in rats. In Trace Elements in Man and Animals – TEMA 5, pp. 456457 [Mills., C. F., Bremner, I. and Chesters, J. K., editors]. Farnham Royal, Slough: CAB.
Davies, N. T. & Nightingale, R. (1975) The effects of phytate on intestinal absorption of zinc, and whole-body retention of Zn, copper, iron and manganese in rats. British Journal of Nutrition 34, 243258.
Davies, N. T. & Olpin, S. E. (1979) Studies on the phytate:zinc molar contents in diets as a determinant of Zn availability to young rats. British Journal of Nutrition 41, 591603.
Davis, P. N., Norris, L. C. & Kratzer, F. H. (1962) Interference of soybean proteins with the utilization of trace minerals. Journal of Nutrition 77, 217223.
Eastwood, W. A. & Kay, R. M. (1979) A hypothesis for the action of dietary fibre along the gastrointestinal tract. American Journal of Clinical Nutrition 32, 364367.
Forbes, R. M. (1984) Use of laboratory animals to define physiological functions and bioavailability of zinc. Federation Proceedings 43, 28352839.
Forbes, R. M., Erdman, J. W. Jr, Parker, H. M., Kondo, H. & Ketelsen, S. M. (1983) Bioavailability of zinc in coagulated soy protein (tofu) to rats and effect of dietary calcium at a constant phytate:zinc ratio. Journal of Nutrition 113, 205210.
Forbes, R. M., Parker, H. M. & Erdman, J. W. Jr (1984) Effects of dietary phytate, calcium and magnesium levels on zinc bioavailability to rats. Journal of Nutrition 114, 14211425.
Forbes, R. M., Weingartner, K. E., Parker, H. M., Bell, R. R. & Erdman, J. W. Jr (1979) Bioavailability to rats of zinc, magnesium and calcium in casein-, egg- and soya protein-containing diets. Journal of Nutrition 109, 16521660.
Gillooly, M., Bothwell, T. H., Torrance, J. D., MacPhail, A. P., Derman, D. P., Bezboda, W. R., Mills, W. & Charlton, R. W. (1983) The effects of organic acids, phytates and polyphenols on the absorption of iron from vegetables.British Journul of Nutrition 49, 331342.
Gitelman, H. J. (1967) An improved automated procedure for the determination of calcium in biological specimens. Analytical Biochemistry 18, 521531.
Gitelman, H. J., Hurt, C. & Lutwak, L. (1966) An automated spectrophotometric method for magnesium analysis. Analytical Biochemistry 14, 106120.
Griffiths, D. W. & Jones, D. I. H. (1977) Cellulase inhibition by tannins in the testa of field beans (Vicia faba). Journal of the Science of Food and Agriculture 28, 983989.
Harland, B. F. & Oberleas, D. (1986) Anion-exchange method for determination of phytate in foods: collaborative study. Journal of the Association of Official Analytical Chemists 69, 667670.
Hellendoorn, E. W. (1979) Beneficial physiological activity of leguminous seeds. Qualitas Plantarum. Plant Foods for Human Nutrition 29, 227244.
Kahn, A., Vohra, P. N. & Kratzer, F. H. (1987) The effect of protein level and dietary guar gum and pectin on copper and zinc utilization in chicks. Nutrition Reports International 36, 193200.
Macarulla, M. T. (1989) Influencia de la ingestion de dietas de Vicia faba L. con diferentes niveles de zinc sobre el estado nutritivo y la respuesta inmune del raton. (Influence of the ingestion of Vicia faba L. diets of different zinc contents on the nutritional state and the immune response of the mouse.) Tesis Doctoral, Universidad de Navarra, Pamplona, Spain.
Martinez, J. A., Barcina, Y. & Larralde, J. (1985) Interrelationships between zinc supply and protein source in young and adult rats. Nutrition Reports International 32, 10371046.
National Advisory Committee on Nutrition Education (1983) Proposals for Nutritional Guidelines for Health Education in Britain. London: Health Education Council.
O'Dell, B. L., Johe, J. M. & Savage, J. E. (1964) Zinc availability in the chick as affected by phytate, calcium and ethylenediaminetetraacetate. Poultry Science 43, 415419.
Pritchard, P. J., Dryburgh, E. A. & Wilson, B. J. (1973) Carbohydrates of spring and winter field beans (Vicia faba L.). Journal of the Science of Food and Agriculture 24, 663668.
Reinhold, J. G., Faradji, B., Abadi, P. & Ismail-Beigi, F. (1976) Decreased absorption of calcium, magnesium, zinc and phosphorus by humans due to increased fiber and phosphorus consumption as wheat bread. Journal of Nutrition 106, 493503.
Roach, A. G. (1965) Application of the Technicon Auto Analyzer equipment to the routine determination of calcium and phosphorus in animal feedstuffs. Automation in Analytical Chemistry, pp. 137144. Basingstoke: Technicon Instruments Co. Ltd.
Rubio, L. A. & Brenes, A. (1988) Plasma mineral concentrations in growing chickens fed diets containing raw and autoclaved faba beans (Vicia faba L.) and faba bean fractions. Nutrition Reports International 38, 609619.
Rubio, L. A., Brenes, A. & Castano, M. (1989) Histological alterations of the pancreas and the intestinal tract produced by raw faba bean (Vicia faba L., minor) diets in growing chicks. British Poultry Science 30, 1528.
Rubio, L. A., Brenes, A. & Castano, M. (1990) The utilization of raw and autoclaved faba beans (Vicia faba L., minor) and faba bean fractions in diets for growing broiler chickens. British Journal of Nutrition 63, 419433.
Rubio, L. A., Grant, G., Bardocz, S., Dewey, P. & Pusztai, A. (1991) Nutritional response of growing rats to faba beans (Vicia faba L., minor) and faba bean fractions. British Journal of Nutrition 66, 533542.
Schiller, K. (1960) Ein Stoffwechselkafig für Raten (A metabolism cage for rats). Zeitschrift für Tierphysiologie, Tierernahrung und Futtermittelkunde 15, 305308.
Sgarbieri, V. C. (1989) Nutritive value of beans. In Nutritional Value of Cereal Products, Beans and Starches, pp. 132199 [Bourne, G. H., editor]. Basel, Switzerland: Karger.
Steel, R. G. D. & Torrie, J. H. (1960) Principles and Procedures of Statistics. New York: McGraw-Hill.
Weingartner, K. E., Erdman, J. W., Parker, H. M. & Forbes, R. M. (1979) Effect of soybean hull upon the bioavailability of zinc and calcium from soy flour-based diets. Nutrition Reports International 19, 223231.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed