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Manganese influences the expression of fatty acid synthase and malic enzyme in cultured primary chicken hepatocytes

  • Lin Lu (a1), Meiling Wang (a1), Xiudong Liao (a1), Liyang Zhang (a1) and Xugang Luo (a1)...

Abstract

Two experiments were designed to investigate the effects of Mn source and concentration on the mRNA expression and enzymatic activities of fatty acid synthase (FAS) and malic enzyme (ME) in cultured primary broiler hepatocytes. In Expt 1, primary broiler hepatocytes were treated with 0 (control), 0·25, 0·50 or 0·75 mmol/l of Mn as inorganic manganese chloride (MnCl2.4H2O) for 24 and 48 h. In Expt 2, primary broiler hepatocytes were incubated with 0 (control), 0·25 or 0·50 mmol/l of Mn as either manganese chloride or Mn–amino acid chelate for 48 h. The mRNA levels and activities of FAS and ME in the hepatocytes were measured in Expts 1 and 2. The results in Expt 1 showed that only at 48 h mRNA expression levels of FAS and ME in the hepatocytes decreased linearly (P<0·001) and quadratically (P<0·02) as supplemental Mn concentrations increased. In Expt 2, compared with the control, Mn supplementation reduced (P<0·01) the activities of FAS, mRNA expression levels of FAS and ME in the hepatocytes, and the efflux of lactic dehydrogenase to the medium. The supplemental Mn at 0·5 mmol/l showed a lower (P<0·03) ME mRNA expression level compared with the Mn group at 0·25 mmol/l. However, Mn source and the interaction between Mn source and concentration had no impacts (P>0·33) on any of the measured cellular parameters. The results suggested that Mn might reduce cell damage and regulate FAS and ME expression at a transcriptional level in primary cultured broiler hepatocytes.

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Copyright

Corresponding author

* Corresponding author: Professor X. Luo, fax +86 10 62810184, email wlysz@263.net

Footnotes

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These authors contributed equally to the present work.

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References

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1. Leveille, GA, Romsos, DR, Yeh, YY, et al. (1975) Lipid biosynthesis in the chick. A consideration of site of synthesis, influence of diet and possible regulatory mechanisms. Poult Sci 54, 10751093.
2. Saadoun, A & Leclercq, B (1987) In vivo lipogenesis of genetically lean and fat chickens: effects of nutritional state and dietary fat. J Nutr 117, 428435.
3. Hermier, D (1997) Lipoprotein metabolism and fattening in poultry. J Nutr 127, 805S.
4. Morris, SM Jr, Winberry, LK, Fisch, JE, et al. (1984) Developmental and nutritional regulation of the messenger RNAs for fatty acid synthase, malic enzyme and albumin in the livers of embryonic and newly-hatched chicks. Mol Cell Biochem 64, 6368.
5. Whitehead, CC, Hood, RL, Heard, GS, et al. (1984) Comparison of plasma very low density lipoproteins and lipogenic enzymes as predictors of fat content and food conversion efficiency in selected lines of broiler chickens. Br Poult Sci 25, 277286.
6. Yang, Y (2005) Studies on the metabolism of intramuscular fat in quality chicken and its relationships with meat quality traits. Doctoral dissertation, Graduate School of Chinese Academy of Agricultural Sciences.
7. Morris, SMJ, Nilson, JH, Jenik, RA, et al. (1982) Molecular cloning of gene sequences for avian fatty acid synthase and evidence for nutritional regulation of fatty acid synthase mRNA concentration. J Biol Chem 257, 32253229.
8. Goodridge, AG, Jenik, RA, Mcdevitt, MA, et al. (1984) Malic enzyme and fatty acid synthase in the uropygial gland and liver of embryonic and neonatal ducklings. Tissue-specific regulation of gene expression. Arch Biochem Biophys 230, 8292.
9. Erikson, KM, Syversen, T, Aschner, JL, et al. (2005) Interactions between excessive manganese exposures and dietary iron-deficiency in neurodegeneration. Environ Toxicol Pharmacol 19, 415421.
10. National Research Council (1994) Nutrient Requirements of Poultry, 9th ed. Washington, DC: National Academies Press.
11. Wilgus, HS, Norris, LC & Heuser, GF (1937) The role of manganese and certain other trace elements in the prevention of perosis. J Nutr 14, 155167.
12. Insko, WM, Lyons, M & Martin, JH (1938) The quantitative requirement of the growing chick for manganese. J Nutr 15, 621627.
13. Gallup, WD & Norris, LC (1939) The amount of manganese required to prevent perosis in the chick. Poult Sci 18, 7682.
14. Luo, XG, Su, Q, Huang, JC & Liu, JX (1991) A study on the optimal manganese (Mn) level in a practical diet of broiler chicks. Chin J Anim Vet Sci 22, 313317.
15. Li, S, Lin, Y, Lu, L, et al. (2011) An estimation of the manganese requirement for broilers from 1 to 21 days of age. Biol Trace Elem Res 143, 939948.
16. Lu, L, Chang, B, Liao, X, et al. (2016) Use of molecular biomarkers to estimate manganese requirements for broiler chickens from 22 to 42 d of age. Br J Nutr 116, 15121518.
17. Plumlee, MP, Thrasher, DM, Beeson, WM, et al. (1956) The effects of a manganese deficiency upon the growth, development, and reproduction of swine. J Anim Sci 15, 352367.
18. Atherton, D (1993) A nutritional approach to carcass leanness. In The Role of Amino Acid Chelates in Animal Nutrition, pp. 269287 [HD Ashmead, editor]. Park Ridge, NJ: Noyes Publication.
19. Lu, L, Ji, C, Luo, XG, et al. (2006) The effect of supplemental manganese in broiler diets on abdominal fat deposition and meat quality. Anim Feed Sci Tech 129, 4959.
20. Lu, L, Luo, XG, Ji, C, et al. (2007) Effect of manganese supplementation and source on carcass traits, meat quality, and lipid oxidation in broilers. J Anim Sci 85, 812822.
21. Wang, M, Chen, Z, Lu, L, et al. (2011) Effect of different manganese sources on activities and gene expression of key enzymes in fat metabolism of broilers. Sci Agri Sin 44, 38503858.
22. Li, S, Luo, X, Liu, B, et al. (2004) Use of chemical characteristics to predict the relative bioavailability of supplemental organic manganese sources for broilers. J Anim Sci 82, 23522363.
23. Fraslin, J, Touquette, L, Douaire, M, et al. (1992) Isolation and long-term maintenance of differentiated adult chicken hepatocytes in primary culture. In Vitro Cell Dev Biol 28, 615620.
24. Wu, X, Huang, K, Wei, C, et al. (2010) Regulation of cellular glutathione peroxidase by different forms and concentrations of selenium in primary cultured bovine hepatocytes. J Nutr Biochem 21, 153161.
25. Fujii, M, Yoshino, I, Suzuki, M, et al. (1996) Primary culture of chicken hepatocytes in serum-free medium (pH 7·8) secreted albumin and transferrin for a long period in free gas exchange with atmosphere. Int J Biochem Cell Biol 28, 1381.
26. Holwerda, RA, Albin, RC & Madsen, FC (1995) Chelation effectiveness of zinc proteinates demonstrated. Feedstuffs 67, 1213, 23.
27. Tian, WX, Hsu, RY & Wang, YS (1985) Studies on the reactivity of the essential sulfhydryl groups as a conformational probe for the fatty acid synthetase of chicken liver. Inactivation by 5,5'-dithiobis-(2-nitrobenzoic acid) and intersubunit cross-linking of the inactivated enzyme. J Biol Chem 260, 1137511387.
28. Hsu, RY & Lardy, HA (1969) Malic enzyme. Methods Enzymol 13, 230235.
29. Li, S, Lin, L, Liao, X, et al. (2016) Manganese elevates manganese superoxide dismutase protein level through protein kinase C and protein tyrosine kinase. Biometals 29, 265274.
30. Livak, KJ & Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25, 402408.
31. Zhu, YW, Lu, L, Li, WX, et al. (2015) Effects of maternal dietary manganese and incubation temperature on hatchability, antioxidant status, and expression of heat shock proteins in chick embryos. J Anim Sci 93, 57255734.
32. Suo, H, Lu, L, Zhang, L, et al. (2015) Relative bioavailability of zinc-methionine chelate for broilers fed a conventional corn-soybean meal diet. Biol Trace Elem Res 146, 181186.
33. Zhang, LY, Lu, L & Luo, XG (2016) The chemical characteristics of organic iron sources and their relative bioavailabilities for broilers fed a conventional corn-soybean meal diet. J Anim Sci 94, 23782396.
34. Mitchell, DB & Acosta, D (1981) Evaluation of the cytotoxicity of tricyclic antidepressants in primary cultures of rat hepatocytes. J Toxicol Environ Health 7, 8392.
35. Mcqueen, CA & Williams, GM (1982) Cytotoxicity of xenobiotics in adult rat hepatocytes in primary culture. Fundam Appl Toxicol 2, 139.
36. Gao, T, Wang, F, Li, S, et al. (2011) Manganese regulates manganese-containing superoxide dismutase (MnSOD) expression in the primary broiler myocardial cells. Biol Trace Elem Res 144, 695704.
37. Lakshmanan, MR, Nepokroeff, CM & Porter, JW (1972) Control of the synthesis of fatty-acid synthetase in rat liver by insulin, glucagon, and adenosine 3′:5′cyclic monophosphate. Proc Natl Acad Sci U S A 69, 35163519.
38. Li, SF, Luo, XG, Lu, L, et al. (2005) Bioavailability of organic manganese sources in broilers fed high dietary calcium. Anim Feed Sci Tech 123–124, 703715.
39. Luo, XG, Li, SF, Lu, L, et al. (2007) Gene expression of manganese-containing superoxide Dismutase as a biomarker of manganese bioavailability for manganese sources in broilers. Poult Sci 86, 888894.
40. Li, SF, Luo, XG, Lu, L, et al. (2008) Effect of intravenously injected manganese on the gene expression of manganese-containing superoxide dismutase in broilers. Poult Sci 87, 22592265.
41. Li, S, Lu, L, Hao, S, et al. (2011) Dietary manganese modulates expression of the manganese-containing superoxide dismutase gene in chickens. J Nutr 141, 189194.
42. Ji, F, Luo, XG, Lu, L, et al. (2006) Effects of manganese source and calcium on manganese uptake by in vitro everted gut sacs of broilers’ intestinal segments. Poult Sci 85, 12171225.
43. Ji, F, Luo, XG, Lu, L, et al. (2006) Effect of manganese source on manganese absorption by the intestine of broilers. Poult Sci 85, 19471952.
44. Yoshimoto, K, Nakamura, T & Ichihara, A (1983) Reciprocal effects of epidermal growth factor on key lipogenic enzymes in primary cultures of adult rat hepatocytes. Induction of glucose-6-phosphate dehydrogenase and suppression of malic enzyme and lipogenesis. J Biol Chem 258, 1235512360.
45. Ma, XJ, Salati, LM, Ash, SE, et al. (1990) Nutritional regulation and tissue-specific expression of the malic enzyme gene in the chicken. Transcriptional control and chromatin structure. J Biol Chem 265, 1843518441.
46. Katsurada, A, Iritani, N, Fukuda, H, et al. (1987) Influence of diet on the transcriptional and post-transcriptional regulation of malic enzyme induction in the rat liver. Eur J Biochem 168, 487491.
47. Goodridge, AG, Crish, JF, Hillgartner, FB, et al. (1989) Nutritional and hormonal regulation of the gene for avian malic enzyme. J Nutr 119, 299308.

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Manganese influences the expression of fatty acid synthase and malic enzyme in cultured primary chicken hepatocytes

  • Lin Lu (a1), Meiling Wang (a1), Xiudong Liao (a1), Liyang Zhang (a1) and Xugang Luo (a1)...

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