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Intestinal absorption of linoleic acid in experimental renal failure

Published online by Cambridge University Press:  09 March 2007

M. V. Pahl ,
A. Barbari ,
N. D. Vaziri ,
D. Hollander ,
M. Yazdani ,
J. Tran  and
R. Kan
M. V. Pahl
Affiliation:
Division of Nephrology, Department of Medicine, University of California at Irvine, Irvine, California 92668, USA
A. Barbari
Affiliation:
Division of Nephrology, Department of Medicine, University of California at Irvine, Irvine, California 92668, USA
N. D. Vaziri
Affiliation:
Division of Nephrology, Department of Medicine, University of California at Irvine, Irvine, California 92668, USA
D. Hollander
Affiliation:
Division of Nephrology, Department of Medicine, University of California at Irvine, Irvine, California 92668, USA
M. Yazdani
Affiliation:
Division of Nephrology, Department of Medicine, University of California at Irvine, Irvine, California 92668, USA
J. Tran
Affiliation:
Division of Nephrology, Department of Medicine, University of California at Irvine, Irvine, California 92668, USA
R. Kan
Affiliation:
Division of Nephrology, Department of Medicine, University of California at Irvine, Irvine, California 92668, USA
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Abstract

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Linoleic acid (LA) transport in rats with experimental short-term and long-term renal failure (RF) was compared with that of sham-operated normal animals on liberal food intake and pair-fed animals. The perfusions in vivo and incubations in vitro were conducted using a micellar solution containing a wide range of LA concentrations. Both absorption in vivo and uptake in vitro of LA were significantly reduced in animals with short-term RF. Lipid extraction and separation by thin-layer chromatography revealed a marked LA trapping as trilinolein (TL) in the perfused intestinal tissue in the short-term RF group. The esterification process, as defined by the rate of LA incorporation into TL, was moderately reduced in short-term RF animals. The thickness of the unstirred water layer showed no significant difference among the groups studied. In contrast, animals with long-term RF exhibited normal absorption of LA in vivo at all concentrations tested. In conclusion, LA absorption is reduced in short-term RF and restored in long-term RF. Several steps including LA transport into and TL transport out of the enterocyte and the esterification process were impaired in short-term RF. These changes are not due to alteration in the unstirred water layer, anorexia, weight loss or a rapid effect of uraemic chemical environment or circulatory factors.

Keywords

Renal failureFatty acid absorptionIntestinal transport
Type
Lipid Metabolism
Information
British Journal of Nutrition , Volume 66 , Issue 3 , November 1991 , pp. 467 - 477
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Anderson, B. W., Levine, A. S., Levitt, D. G., Kneip, J. M. & Levitt, M. D. (1988). Physiological measurement of luminal stirring in perfused rat jejunum. American Journal of Physiology 254, G843–G848.Google ScholarPubMed
Bennet Clark, S., Ekkers, T. E., Singh, A., Balint, J. A., Holt, P. R. & Rodgers, J. B. Jr (1973). Fat absorption in essential fatty acid deficiency: A model experimental approach to studies of the mechanism of fat malabsorption of unknown etiology. Journal of Lipid Research 14, 581–588.CrossRefGoogle Scholar
Brown, J. L. & Johnston, J. M. (1962). Radioassay of lipid components separated by thin layer chromatography. Journal of Lipid Research 3, 480–481.CrossRefGoogle Scholar
Chow, S. L. & Hollander, D. (1979 a). A duel, concentration-dependent absorption mechanism of linoleic acid by rat jejunum in vitro. Journal of Lipid Research 20, 349–356.CrossRefGoogle Scholar
Chow, S. & Hollander, D. (1979 b). Linoleic acid absorption in the unanesthetized rat: Mechanism of transport and influence of luminal factors on absorption. Lipids 14, 378–385.CrossRefGoogle ScholarPubMed
Delano, B. G., Manis, J. G. & Manis, T. (1977). Iron absorption in experimental uremia. Nephron 19, 26–31.CrossRefGoogle ScholarPubMed
Draper, N. R. & Smith, J. (1966). Applied Regression Analysis, pp. 1–43. New York: John Wiley & Sons.Google Scholar
Hollander, D. (1981). Intestinal absorption of vitamin A, E, D and K. Journal of Laboratory Medicine 97, 449–462.Google Scholar
Kates, M. (1975). Techniques of lipidology: Isolation, analysis and identification of lipids. Laboratory Techniques in Biochemistry and Molecular Biology, 2nd ed., vol. 6, pp. 349–350. New York: Elsevier Science Publishing Co.Google Scholar
Kessler, J. I., Mishkin, S. & Stein, J. (1969). Effect of dl-ethionine on the intestinal absorption and transport of palmitic acid-l-14C and tripalmitic 14C. Role of intramucosal factors in the uptake of luminal lipids. Journal of Clinical Investigation 48, 1397–1407.CrossRefGoogle Scholar
Lohrs, U., Castrup, H. J., Wiebecke, B. & Eder, M. (1973). Effects of experimental uraemia on the small intestinal epithelium. Proceedings of the International Conference on Anatomy, Physiology and Biochemistry of Intestinal Adaptation, pp. 159–169. Stuttgart: Schattauer.Google Scholar
McManus, J. P. A. & Isselbacher, K. J. (1970). Effect of fasting versus refeeding on the rat small intestine: Morphological, biochemical and functional differences. Gastroenterology 59, 214–221.CrossRefGoogle Scholar
Marubbio, A. T. Jr, Morris, J. S. Jr, Bennet Clark, S. & Holt, P. R. (1974). Monoglyceride modification of jejunal absorption of fatty acid in the rat. Journal of Lipid Research 15, 165–172.CrossRefGoogle ScholarPubMed
Ockner, R. K. & Isselbacher, K. J. (1974). Recent concepts of intestinal fat absorption. Review of Physiology, Biochemistry & Pharmacology 71, 107–146.CrossRefGoogle Scholar
Ockner, R. K., Pittman, J. P. & Yager, J. L. (1972). Differences in the intestinal absorption of saturated and unsaturated long chain fatty acids. Gastroenterology 62, 981–992.CrossRefGoogle ScholarPubMed
Pahl, M. V., Erickson, R. A., Vaziri, N. D., Khamiseh, G., Oveisi, F., Jam, K., Seo, J. & Kutchemeshgi, M. (1990). Intestinal morphometry and bile acid-induced mucosal injury in chronic experimental renal failure. Journal of Laboratory and Clinical Medicine 115, 572–578.Google ScholarPubMed
Powell, G. K. & McElveen, M. A. (1974). Effect of prolonged fasting on fatty acid re-esterification in rat intestinal mucosa. Biochemistry 369, 8–15.Google ScholarPubMed
Rodgers, J. B., Riley, E. M., Drummey, G. D. & Isselbacher, K. J. (1967). Lipid absorption in adrenalectomized rats: The role of altered enzymatic activity in the intestinal mucosa. Gastroenterology 53, 547–556.CrossRefGoogle Scholar
Said, H., Vaziri, N. D., Kariger, R. & Hollander, D. (1984). Intestinal absorption of 5-methyltetrahydrofolate in experimental uremia. Acta Vitaminologica et Enzymologica 6, 339–346.Google ScholarPubMed
Sterner, G., Lindberg, T. & Deenneberg, T. (1982). In vivo and in vitro absorption of amino acids and dipeptides in small intestine of uremic rats. Nephron 31, 273–276.CrossRefGoogle ScholarPubMed
Vaziri, N. D., Hollander, D., Hung, E., Vo, M. & Dadufalza, L. (1983). Impaired intestinal absorption of vitamin D-3 in azotemic rats. American Journal of Clinical Nutrition 37, 273–276.CrossRefGoogle ScholarPubMed
Vaziri, N. D., Said, H., Hollander, D., Kariger, R., Patel, N. & Barbari, A. (1985). Impaired intestinal absorption of riboflavin in experimental uremia. Nephron 41, 26–29.CrossRefGoogle ScholarPubMed
Vergroesen, A. J. (1977). Physiological effects of dietary linoleic acid. Nutrition Reviews 35, 1–5.CrossRefGoogle ScholarPubMed