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The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum (Sciaenops ocellatus) and hybrid striped bass (Morone chrysops× M. saxatilis)

  • Maritza Anguiano (a1), Camilo Pohlenz (a1) (a2), Alejandro Buentello (a1) (a2) and Delbert M. Gatlin (a1) (a2)

Abstract

The effects of four prebiotics (fructo-oligosaccharide, Bio-MOS®, transgalacto-oligosaccharide and GroBiotic®-A) on digestive enzymes and intestinal morphology were studied in juvenile hybrid striped bass (Morone chrysops×M. saxatilis) and red drum (Sciaenops ocellatus) using two separate 8-week feeding trials. Red drum were fed experimental diets with the four prebiotics each individually supplemented at 1 % and hybrid striped bass were fed diets supplemented with GroBiotic®-A at 1 and 2 %. Both trials were conducted with each diet fed to apparent satiation twice per d to three replicate groups of fifteen juvenile fish. For histomorphological analysis, gastrointestinal tract (GIT) samples from three randomly selected fish per tank were taken at 4 and 8 weeks for hybrid striped bass and at 8 weeks for red drum. For both trials, GIT samples from two randomly selected fish per tank were taken at 4 and 8 weeks and analysed for pepsin, trypsin, chymotrypsin, aminopeptidase, α-amylase, lipase, and both acid and alkaline phosphatase activities. The results of the histological evaluation indicated that the inclusion of prebiotics was adequate to elicit structural changes in the GIT of both species. On the other hand, no significant changes in the enzyme activities were detected at week 8 in both species. However, there was a transient effect of Bio-MOS® supplementation on the activities of aminopeptidase, α-amylase and alkaline phosphatase at week 4 in red drum only. Thus, previously observed improvements in nutrient digestibility by these fish in response to prebiotic supplementation appear to be mostly related to changes in GIT structure as opposed to the enhancement of digestive enzyme activity.

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Corresponding author

*Corresponding author: D. M. Gatlin, fax +1 979 845 4096, E-mail: d-gatlin@tamu.edu

References

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1FAO Fisheries Department (2010) The State of the World Fisheries and Aquacultures 2010. Rome: Food and Agriculture Organization of the United Nations. http://www.fao.org/docrep/013/i1820e00.htm.
2Balcazar, JL, de Blas, I, Ruiz-Zarzuela, I, et al. (2006) The role of probiotics in aquaculture. Vet Microbiol 114, 173186.
3Buentello, JA, Neill, WH & Gatlin, DM III (2010) Effects of dietary prebiotics on the growth, feed efficiency and non-specific immunity of juvenile red drum Sciaenops ocellatus fed soybean-based diets. Aquac Res 41, 411418.
4Cummings, JH & Macfarlane, GT (2002) Gastrointestinal effects of prebiotics. Br J Nutr 87, S145S151.
5Burr, G, Gatlin, D III & Ricke, S (2005) Microbial ecology of gastrointestinal tract of fish and the potential application of prebiotics and probiotics in finfish aquaculture. J World Aquac Soc 36, 425436.
6Kelly, G (2008) Inulin-type prebiotics – a review: part 1. Altern Med Rev 13, 315329.
7Xu, B, Wang, Y, Li, J, et al. (2009) Effect of prebiotic xylooligosaccharides on growth performances and digestive enzyme activities of allogynogenetic crucian carp (Carassius auratus gibelio). Fish Physiol Biochem 35, 351357.
8Burr, G, Hume, M, Ricke, S, et al. (2010) In vitro and in vivo evaluation of the prebiotics GroBiotic-A, inulin, mannanoligosaccharide, and galactooligosaccharide on the digestive microbiota and performance of hybrid striped bass (Morone chrysops × Morone saxatilis). Microb Ecol 59, 187198.
9Gibson, GR & Roberfroid, MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 125, 14011412.
10Xu, ZR, Zou, XT, Hu, CH, et al. (2002) Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of growing pigs. Asian-Austral J Anim 15, 17841789.
11Xu, ZR, Hu, CH, Xia, MS, et al. (2003) Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poultry Sci 82, 10301036.
12Yang, Y, Iji, PA, Kocher, A, et al. (2007) Effects of mannanoligosaccharide on growth performance, the development of gut microflora, and gut function of broiler chickens raised on new litter. J Appl Poultry Res 16, 280288.
13Li, P & Gatlin, DM (2004) Dietary brewers yeast and the prebiotic Grobiotic™ AE influence growth performance, immune responses and resistance of hybrid striped bass (Morone chrysops × M. saxatilis) to Streptococcus iniae infection. Aquaculture 231, 445456.
14Li, P & Gatlin, DM (2005) Evaluation of the prebiotic Grobiotic®-A and brewers yeast as dietary supplements for sub-adult hybrid striped bass (Morone chrysops × M. saxatilis) challenged in situ with Mycobacterium marinum. Aquaculture 248, 197205.
15Genc, MA, Yilmaz, E, Genc, E, et al. (2007) Effects of dietary mannan oligosaccharides (MOS) on growth, body composition, and intestine and liver histology of the hybrid tilapia (Oreochromis niloticus× O. aureus). Isr J Aquacult-Bamid 59, 1016.
16Dimitroglou, A, Merrifield, DL, Moate, R, et al. (2009) Dietary mannan oligosaccharide supplementation modulates intestinal microbial ecology and improves gut morphology of rainbow trout. Oncorhynchus mykiss (Walbaum). J Anim Sci 87, 32263234.
17Dimitroglou, A, Davies, SJ, Sweetman, J, et al. (2010) Dietary supplementation of mannan oligosaccharide on white sea bream (Diplodus sargus L.) larvae: effects on development, gut morphology and salinity tolerance. Aquac Res 41, e245e251.
18Dimitroglou, A, Merrifield, DL, Spring, P, et al. (2010) Effects of mannan oligosaccharide (MOS) supplementation on growth performance, feed utilisation, intestinal histology and gut microbiota of gilthead sea bream (Sparus aurata). Aquaculture 300, 182188.
19Zhou, Q-C, Buentello, JA & Gatlin, DM (2010) Effects of dietary prebiotics on growth performance, immune response and intestinal morphology of red drum (Sciaenops ocellatus). Aquaculture 309, 253257.
20Merrifield, DL, Dimitroglou, A, Foey, A, et al. (2010) The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture 302, 118.
21RingØ, E, Olsen, RE, Gifstad, , et al. (2010) Prebiotics in aquaculture: a review. Aquac Nutr 16, 117136.
22Yousefian, M & Amiri, MS (2009) A review of the use of prebiotic in aquaculture for fish and shrimp. Afr J Biotechnol 8, 7713–7318.
23Burr, G, Hume, M, Neill, WH, et al. (2008) Effects of prebiotics on nutrient digestibility of a soybean-meal-based diet by red drum Sciaenops ocellatus (Linnaeus). Aquac Res 39, 16801686.
24Pryor, GS, Royes, JB, Chapman, FA, et al. (2003) Mannanoligosaccharides in fish nutrition: effects of dietary supplementation on growth and gastrointestinal villi structure in Gulf of Mexico sturgeon. N Am J Aquacult 65, 106111.
25Yilmaz, E, Genc, MA & Genc, E (2007) Effects of dietary mannan oligosaccharides on growth, body composition, and intestine and liver histology of rainbow trout, Oncorhynchus mykiss. Isr Aquacult-Bamid 59, 182188.
26Moon, HY & Gatlin, DM III (1991) Total sulfur amino acid requirement of juvenile red drum. Sciaenops ocellatus. Aquaculture 95, 97106.
27Anson, ML (1938) The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin. J Gen Physiol 22, 7989.
28Erlanger, BF, Kokowsky, N & Cohen, W (1961) The preparation of properties of two new chromogenic substrates of trypsin. Arch Biochem Biophys 95, 271278.
29Asgeirsson, B & Bjarnason, JB (1991) Structural and kinetic properties of chymotrypsin from Atlantic cod (Gadus morhua). Comparison with bovine chymotrypsin. Comp Biochem Physiol 99B, 327335.
30Vega-Villasante, F, Nolasco, H & Civera, R (1993) The digestive enzymes of the pacific brown shrimp Penaeus californiensis. I – properties of amylase activity in the digestive tract. Comp Biochem Physiol 106B, 547550.
31Versaw, WK, Cuppett, SL, Winters, DD, et al. (1989) An improved colorimetric assay for bacterial lipase in nonfat dry milk. J Food Sci 54, 15571559.
32Refstie, S, Bakke-McKellep, AM, Penn, MH, et al. (2006) Capacity for digestive hydrolysis and amino acid absorption in Atlantic salmon (Salmo salar) fed diets with soybean meal or inulin with or without addition of antibiotics. Aquaculture 261, 392406.
33Salze, G, McLean, E, Schwarz, MH, et al. (2008) Dietary mannan oligosaccharide enhances salinity tolerance and gut development of larval cobia. Aquaculture 274, 148152.

Keywords

The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum (Sciaenops ocellatus) and hybrid striped bass (Morone chrysops× M. saxatilis)

  • Maritza Anguiano (a1), Camilo Pohlenz (a1) (a2), Alejandro Buentello (a1) (a2) and Delbert M. Gatlin (a1) (a2)

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