Skip to main content Accessibility help
×
Home

Effects of intestinal resection, cholecalciferol and ascorbic acid on iron metabolism in rats

  • S. Hartiti (a1), M. Barrionuevo (a1) (a2), I. López-Aliaga (a1), F. Lisbona (a1) (a2), I. Pallarés (a1), M. J. M. Alférez (a1), A. E. Gómez-Ayala (a1) and M. S. Campos (a1) (a2)...

Abstract

The effect of dietary supplementation with ascorbic acid or cholecalciferol on Fe utilization was studied using the metabolic balance technique, in rats in which 50% of the distal small intestine was removed, or in which the mid small intestine was transected and reanastomosed (controls). Three different diets were used. The first (basal diet) contained (g/kg dry wt): protein (casein+50 mg D, L-methionine/g) 120 and fat (medium-chain triacylglycerols, olive oil and sunflower oil, in equal parts) 40. The other diets were obtained by adding ascorbic acid (150 mg/kg diet) or cholecalciferol (0·4 mg/kg diet) to the basal diet. Apparent digestibility coefficient (ADC) and Fe retention were significantly lower in resected animals than in their respective control groups (transected rats). However, the addition of ascorbic acid or cholecalciferol to the basal diet increased the ADC and Fe retention in both transected and resected rats. Five weeks after surgery, resection also resulted in a reduced concentration of Fe in the sternum, but did not reduce the concentration of haemoglobin or serum Fe total Fe-binding capacity or the concentration of Fe in liver, testes, femur or muscle (longissimus dorsi). Supplementation with ascorbic acid increased serum Fe concentration, while the concentration of Fe in muscle was reduced by supplementation with both ascorbic acid and cholecalciferol. Neither supplementation had any effect on the Fe concentration in other tissues, on haemoglobin concentration or plasma total Fe-binding capacity. Thus, supplementation with ascorbic acid or with cholecalciferol increased Fe absorption and reduced the concentration of Fe in muscle.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Effects of intestinal resection, cholecalciferol and ascorbic acid on iron metabolism in rats
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Effects of intestinal resection, cholecalciferol and ascorbic acid on iron metabolism in rats
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Effects of intestinal resection, cholecalciferol and ascorbic acid on iron metabolism in rats
      Available formats
      ×

Copyright

References

Hide All
American Institute of Nutrition (1977) Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. Journal of Nutrition 107, 13401348.
Antonson, D. L. & Vanderhoof, J. A. (1982) Zinc absorption following massive small-bowel resection in the rat. Digestive Diseases and Sciences 27, 789793.
Aziz, N. & Munro, H. N. (1986) Both subunits of rat liver ferritin are regulated at a translational level by iron induction. Nucleic Acids Research 14, 915927.
Barrionuevo, M., Campos, M. S., López-Aliaga, I., Coves, F. & Lisbona, F. (1989) Nutritive utilisation of phosphorus in the rat: influence of intestinal resection and dietary medium chain triglycerides and vitamin D2. International Journal for Vitamin and Nutrition Research 59, 255261.
Beinfait, H. F. & Van den Briel, M. L. (1981) Rapid mobilization of ferritin iron by ascorbate in the presence of oxygen. Biochimica et Biophysica Acta 631, 507511.
Biasco, G., Gallgari, C., Lami, F., Minarini, A., Miglioli, M. & Barbara, L. (1984) Intestinal morphological changes during oral refeeding in a patient previously treated with total parenteral nutrition for small bowel resection. American Journal of Gastroenterology 79, 585588.
Bothwell, T. H., Baynes, R. D., MacFarlane, B. J. & MacPhail, A. P. (1989) Nutritional iron requirements and food iron absorption. Journal of Internal Medicine 226, 357365.
Campos, M. S., López-Aliaga, I., Barrionuevo, M., Lisbona, F. & Coves, F. (1989) Nutritive utilisation of calcium in rats: effects of dietary fat components and vitamin D3, on intestinal resected rats. Journal of Nutritional Science and Vitaminology 35, 511521.
Charles, River Laboratories (1982) Technical Bulletin, Vol. 1, no, 2. Wilmington, MA: Charles River Laboratories.
Conrad, M. E. & Barton, J. C. (1981) Factors affecting iron balance. American Journal of Hematology 10, 199207.
Conrad, M. E., Parmley, R. T. & Osterloh, K. (1987) Small intestinal regulation of iron absorption in the rat. Journal of Laboratory and Clinical Medicine 110, 418426.
Conrad, M. E. & Umbreit, J. N. (1993) A concise review: iron absorption-the mucin-mobilferrin-integrin pathway. A competitive pathway for metal absorption. American Journal of Hematology 42, 6773.
Conrad, M. E., Umbreit, J. N., Moore, E. G., Peterson, R. D. A. & Jones, M. B. (1990) A newly identified iron binding protein in duodenal mucosa of rats. Purification and characterization of mobilferrin. Journal of Biological Chemistry 265, 52735277.
Conrad, M. E., Umbreit, J. N., Moore, E. G. & Rodning, C. R. (1992) Newly identified iron binding protein in human duodenal mucosa. Blood 79, 244247.
Cook, J. D., Dassenko, S. A. & Whittaker, P. (1991) Calcium supplementation: effect on iron absorption. American Journal of Clinical Nutrition 53, 106111.
Coves, F., Lisbona, F., Campos, M. S., García, J. R., López–Aliaga, I. & Barrionuevo, M. (1991 a) Influence of intestinal resection, type of dietary fat and time on the digestive and metabolic utilization of fat in rats. Biomedica et Biochimica Acta 50, 285292.
Coves, F., López-Aliaga, I., Campos, M. S., Lisbona, F. & Barrionuevo, M. (1991 b) Influence of intestinal resection and quality of dietary fat on nutritive utilisation of fat. Revista Española de Enfermedades Digestivas 79, 914.
Dandekar, T., Stripecke, R., Gray, N. K., Goossen, B., Constable, A., Johansson, H. E. & Hentze, M. W. (1991) Identification of a novel iron-responsive element in murine and human erythroid delta-aminolevulinic acid synthase mRNA. EMBO Journal 10, 19031909.
Devine, R. M. & Kelly, K. A. (1989) Surgical therapy of the short bowel syndrome. Gastroenterology Clinics of North America 18, 603618.
Ford, W. D. A., Vries, J. E., Ross, J. S. & Malt, R. A. (1985) Effect of luminal contents on postresectional longitudinal and mucosal growth in the ileum of suckling rats. Surgery 98, 935941.
Gómez-Ayala, A. E., Lisbona, F., López-Aliaga, I., Barrionuevo, M., Pallarés, I., Alférez, M. J. M., Hartiti, S. & Campos, M. S. (1994) Effect of intestinal resection, type of dietary fat and time on biliary lipid secretion in rats. Experimental Physiology 79, 2533.
Grey, V. L. & Morin, C. L. (1985) Evidence for a growth-stimulating fraction in the rat proximal intestine after small bowel resection. Gastroenterology 89, 13051312.
Hallberg, L., Brune, M. & Rossander, L. (1989) The role of vitamin C in iron absorption. International Journal for Vitamin and Nutrition Research 30, (Suppl.), 103108.
Hallberg, L., Brune, M. & Rossander-Hulthen, L. (1987) Is there a physiological role of vitamin C in iron absorption? Annals of the New York Academy of Sciences 498, 324332.
Hanson, W. R., Osborne, J. W. & Sharp, J. G. (1977 a) Compensation by the residual intestine after intestinal resection in the rat. I. Influence of amount of tissue removed. Gastroenterology 72, 692700.
Hanson, W. R., Oshorne, J. W. & Sharp, J. G. (1977 b) Compensation by the residual intestine after intestinal resection in the rat. II. Influence of postoperative time interval. Gastroenterology 72, 701705.
Hazel, C., McCulloch, C. & Bjerknes, M. (1986) Effects of 30% intestinal resection on whole population cell kinetics of mouse intestinal epithelium. Anatomical Record 215, 3541.
Hoffman, K. E., Yanelli, K. & Bridges, K. R. (1991) Ascorbic acid and iron metabolism: alterations in lysosomal functions. American Journal of Clinical Nutrition 54, 1188S1192S.
Huebers, H. A., Beguin, Y., Pootrakul, P., Einspahr, D. & Finch, C. A. (1990) Intact transferrin receptors in human plasma and their relation to erythropoiesis. Blood 75, 102107.
Huebers, H. A. & Finch, C. A. (1987) The physiology of transferrin and transferrin receptors. Physiological Reviews 67, 520582.
Kochanowski, B. A. & McMahan, C. L. (1990) Inhibition of iron absorption by calcium in rats and dogs: effects of mineral separation by time and enteric coating. Nutrition Research 10, 219226.
Kühn, L. C. (1991) mRNA-protein interactions regulate critical pathways in cellular iron metabolism. British Journal of Haematology 79, 15.
Kühn, L. C., Schulman, H. M. & Ponka, P. (1990) Iron-transferrin requirements and transferrin receptor expression in proliferating cells. In Iron Transport and Storage, pp. 149199 [Ponka, P., Schulman, H. M. and Woodworth, R. C., editors]. Boca Raton, Florida: CRC Press.
Kwan, W. C., Quamne, C. A. & Freeman, H. J. (1987) Sodium dependent D-glucose transport in brush-border membrane vesicles following massive distal small bowel resection in the rat. Gastroenterology 92, 19871993.
Lisbona, F., Campos, M. S., Coves, F., Garcia, J. A., Barrionuevo, M. & López-Alidga, I. (1991) Influence of ileal resection, type of diet and ursodeoxycholic acid on biliary secretion in rats. Experimental Physiology 76, 567572.
López-Aliaga, I., Barrionuevo, M., Campos, M. S., Coves, F. & Lisbona, F. (1991) Influence of intestinal resection and type of diet on digestive utilisation and metabolism of magnesium in rats. International Journal for Vitamin and Nutrition Research 61, 6166.
May, B. K., Bhasker, C. R., Bawden, M. J. & Cox, T. C. (1990) Molecular regulation of 5-aminolevulinate synthase. Diseases related to heme biosyuthesis. Molecular Biology and Medicine 7, 405421.
Mugitani, K. (1989) Detection of transferrin in the digestive juices of rat and its possible role in iron absorption from the small intestine. Journal of the Nippon Medical School 65, 144145.
Muir, A. & Hopfer, U. (1985) Regional specificity of iron uptake by small intestinal brush-border membranes from normal and iron-deficient mice. American Journal of Physiology 248, G376G379.
Pallarés, I., Lisbona, F., López-Aliaga, I., Barrionuevo, M., Alférez, M. J. M. & Campos, M. S. (1993) Effect of iron deficiency on the digestive utilization of iron, phosphorus, calcium and magnesium in rats. British Journal of Nutrition 70, 609620.
Ramsay, W. N. M. (1957) Determination of the total iron-binding capacity of serum. Clinica Chimica Acta 2, 221226.
Refsum, S. B. & Schreiner, B. B. (1984) Regulation of iron balance by absorption and excretion. A critical review and a new hypothesis. Scandinavian Journal of Gastroenterology 19, 867874.
Schümann, K., Elsenhans, B., Ehtechami, C. & Forth, W. (1990) Rat intestinal iron transfer capacity and the longitudinal distribution of its adaptation to iron deficiency. Digestion 46, 3545.
Shull, G. E. & Theil, E. C. (1982) Translational control of ferritin synthesis by iron in embryonic reticulocytes of the bullfrog. Journal of Biological Chemistry 257, 1418714191.
Tanaka, H. & Teitelbaum, S. L. (1990) Vitamin D regulates transferrin receptor expression by bone marrow macrophage precursors. Journal of Cellular Physiology 145, 303309.
Thomas, K. & Mitchell, H. H. (1923) A method of determining the biological value of protein. Journal of Biological Chemistry 58, 873903.
Trinder, P. (1956) The improved determination of iron in serum. Journal of Clinical Pathology 9, 170172.
Wilson, H. D., Miller, T., Ogesen, B., Schedl, H. P., Failla, M. L. & Loven, D. P. (1986) Adaptation of the duodenum and ileum of the rat mid-gut resection: enzyme activity and trace metal status. American Journal of Clinical Nutrition 43, 185193.
Wittmann, T., Crenner, F., Pousse, A. & Grenier, J. F. (1985) Changes in motility after jejunal and ileal resection: electromyographic study in rats. Digestion 32, 114123.
Zhang, D., Hendricks, D. G. & Mahoney, A. W. (1989) Bioavailability of total iron from meat, spinach (Spinacea oleracea L.) and meat-spinach mixtures by anaemic and non-anaemic rats. British Journal of Nutrition 61, 331343.

Keywords

Effects of intestinal resection, cholecalciferol and ascorbic acid on iron metabolism in rats

  • S. Hartiti (a1), M. Barrionuevo (a1) (a2), I. López-Aliaga (a1), F. Lisbona (a1) (a2), I. Pallarés (a1), M. J. M. Alférez (a1), A. E. Gómez-Ayala (a1) and M. S. Campos (a1) (a2)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed