Skip to main content Accessibility help
×
Home

Effect of the dietary delivery matrix on vitamin D3 bioavailability and bone mineralisation in vitamin-D3-deficient growing male rats

  • Alison J. Hodgkinson (a1), Olivia A. M. Wallace (a1), Marlena C. Kruger (a2) and Colin G. Prosser (a3)

Abstract

This study assessed bioavailability and utilisation of vitamin D3 in two feeding trials using young, growing Sprague–Dawley male rats. Trial one fed animals standard AIN-93G diet (casein protein) containing no vitamin D3 and goat or cow skimmed milk supplemented with vitamin D3. Trial two fed animals modified dairy-free AIN-93G diet (egg albumin) containing no vitamin D3 and goat or cow skimmed or full-fat milk supplemented with vitamin D3. Control groups received AIN-93G diets with or without vitamin D, and water. At 8 weeks of age, blood samples were collected for vitamin and mineral analysis, and femurs and spines were collected for assessment of bone mineralisation and strength. In both trials, analyses showed differences in bioavailability of vitamin D3, with ratios of serum 25-hydroxyvitamin D3 to vitamin D3 intake more than 2-fold higher in groups drinking supplemented milk compared with groups fed supplemented solid food. Bone mineralisation was higher in groups drinking supplemented milk compared with groups fed supplemented solid food, for both trials (P<0·05). There was no difference in the parameters tested between skimmed milk and full-fat milk or between cow milk and goat milk. Comparison of the two trials suggested that dietary protein source promoted bone mineralisation in a growing rat model: modified AIN-93G with egg albumin produced lower bone mineralisation compared with standard AIN-93G with casein. Overall, this study showed that effects of vitamin D3 deficiency in solid diets were reversed by offering milk supplemented with vitamin D3, and suggests that using milk as a vehicle to deliver vitamin D is advantageous.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Effect of the dietary delivery matrix on vitamin D3 bioavailability and bone mineralisation in vitamin-D3-deficient growing male rats
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Effect of the dietary delivery matrix on vitamin D3 bioavailability and bone mineralisation in vitamin-D3-deficient growing male rats
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Effect of the dietary delivery matrix on vitamin D3 bioavailability and bone mineralisation in vitamin-D3-deficient growing male rats
      Available formats
      ×

Copyright

Corresponding author

* Corresponding author: A. J. Hodgkinson, fax +64 7 838 5611, email ali.hodgkinson@agresearch.co.nz

References

Hide All
1. Spiro, A & Buttriss, JL (2014) Vitamin D: an overview of vitamin D status and intake in Europe. Nutr Bull 39, 322350.
2. Calvo, MS, Whiting, SJ & Barton, CN (2005) Vitamin D intake: a global perspective of current status. J Nutr 135, 310316.
3. Tripkovic, L, Lambert, H, Hart, K, et al. (2012) Comparison of vitamin D2 and vitamin D3 supplementation in raising serum 25-hydroxyvitamin D status: a systematic review and meta-analysis. Am J Clin Nutr 95, 13571364.
4. Haussler, MR (1986) Vitamin D receptors: nature and function. Annu Rev Nutr 6, 527562.
5. Holick, MF (2007) Vitamin D deficiency. N Engl J Med 357, 266281.
6. Holick, MF, Binkley, NC, Bischoff-Ferrari, HA, et al. (2011) Evaluation, treatment, and prevention of vitamin D deficiency: an Endocrine Society clinical practice guideline. J Clin Endocrinol Metab 96, 19111930.
7. Thacher, TD & Clarke, BL (2011) Vitamin D insufficiency. Mayo Clin Proc 86, 5060.
8. Maguire, JL, Birken, CS, Khovratovich, M, et al. (2013) Modifiable determinants of serum 25-hydroxyvitamin D status in early childhood: opportunities for prevention. JAMA Pediatrics 167, 230235.
9. Laaksi, IT, Ruohola, JS, Ylikomi, TJ, et al. (2006) Vitamin D fortification as public health policy: significant improvement in vitamin D status in young Finnish men. Eur J Clin Nutr 60, 10351038.
10. Calvo, MS & Whiting, SJ (2013) Survey of current vitamin D food fortification practices in the United States and Canada. J Steroid Biochem Mol Biol 136, 211213.
11. Lee, GJ, Birken, CS, Parkin, PC, et al. (2014) Consumption of non–cow’s milk beverages and serum vitamin D levels in early childhood. CMAJ 186, 12871293.
12. Fox, MK, Condon, E, Briefel, RR, et al. (2010) Food consumption patterns of young preschoolers: are they starting off on the right path? J Am Diet Assoc 110, S52S59.
13. Goldbohm, RA, Rubingh, CM, Lanting, CI, et al. (2016) Food consumption and nutrient intake by children aged 10 to 48 months attending day care in The Netherlands. Nutrients 8, 428.
14. Vanderhout, SM, Birken, CS, Parkin, PC, et al. (2016) Higher milk fat content is associated with higher 25-hydroxyvitamin D concentration in early childhood. Appl Physiol Nutr Metab 41, 516521.
15. Vanderhout, SM, Birken, CS, Parkin, PC, et al. (2016) Relation between milk-fat percentage, vitamin D, and BMI z score in early childhood. Am J Clin Nutr 104, 16571664.
16. Hunt, JR, Hunt, CD, Zito, CA, et al. (2008) Calcium requirements of growing rats based on bone mass, structure, or biomechanical strength are similar. J Nutr 138, 14621468.
17. Viguet-Carrin, S, Hoppler, M, Membrez Scalfo, F, et al. (2014) Peak bone strength is influenced by calcium intake in growing rats. Bone 68, 8591.
18. McKinnon, H, Kruger, M, Prosser, C, et al. (2010) The effect of formulated goats’ milk on calcium bioavailability in male growing rats. J Sci Food Agric 90, 112116.
19. Li, Y, Seifert, MF, Lim, SY, et al. (2010) Bone mineral content is positively correlated to n-3 fatty acids in the femur of growing rats. Br J Nutr 104, 674685.
20. Anderson, PH, Sawyer, RK, May, BK, et al. (2007) 25-Hydroxyvitamin D requirement for maintaining skeletal health utilising a Sprague-Dawley rat model. J Steroid Biochem Mol Biol 103, 592595.
21. Hohman, EE, Martin, BR, Lachcik, PJ, et al. (2011) Bioavailability and efficacy of vitamin D2 from UV-irradiated yeast in growing, vitamin D-deficient rats. J Agric Food Chem 59, 23412346.
22. Lester, GE, VanderWiel, CJ, Gray, TK, et al. (1982) Vitamin D deficiency in rats with normal serum calcium concentrations. Proc Natl Acad Sci U S A 79, 47914794.
23. Anderson, PH, Sawyer, RK, Moore, AJ, et al. (2008) Vitamin D depletion induces RANKL-mediated osteoclastogenesis and bone loss in a rodent model. J Bone Miner Res 23, 17891797.
24. Indyk, H & Woollard, DC (1985) The determination of vitamin D in fortified milk powders and infant formulas by HPLC. J Micronutr Anal 1, 121141.
25. Brubacher, G, Muller-Mulot, W & Southgate, DAT (1986) Methods for the Determination of Vitamins in Food: Recommended by COST 91. London: Elsevier Science Publishers.
26. Lankes, U, Elder, PA, Lewis, JG, et al. (2015) Differential extraction of endogenous and exogenous 25-OH-vitamin D from serum makes the accurate quantification in liquid chromatography-tandem mass spectrometry assays challenging. Ann Clin Biochem 52, 151160.
27. Hollander, D, Muralidhara, K & Zimmerman, A (1978) Vitamin D-3 intestinal absorption in vivo: influence of fatty acids, bile salts, and perfusate pH on absorption. Gut 19, 267272.
28. Dawson‐Hughes, B, Harris, SS, Palermo, NJ, et al. (2013) Meal conditions affect the absorption of supplemental vitamin D3 but not the plasma 25‐hydroxyvitamin D response to supplementation. J Bone Miner Res 28, 17781783.
29. Dawson-Hughes, B, Harris, SS, Lichtenstein, AH, et al. (2015) Dietary fat increases vitamin D-3 absorption. J Acad Nutr Diet 115, 225230.
30. Tangpricha, V, Koutkia, P, Rieke, SM, et al. (2003) Fortification of orange juice with vitamin D: a novel approach for enhancing vitamin D nutritional health. Am J Clin Nutr 77, 14781483.
31. Sidnell, A, Pigat, S, Gibson, S, et al. (2016) Nutrient intakes and iron and vitamin D status differ depending on main milk consumed by UK children aged 12–18 months–secondary analysis from the Diet and Nutrition Survey of Infants and Young Children. J Nutr Sci 5, e32.
32. Houghton, LA, Gray, AR, Szymlek-Gay, EA, et al. (2011) Vitamin D-fortified milk achieves the targeted serum 25-hydroxyvitamin D concentration without affecting that of parathyroid hormone in New Zealand toddlers. J Nutr 141, 18401846.
33. Masarwi, M, Gabet, Y, Dolkart, O, et al. (2016) Skeletal effect of casein and whey protein intake during catch-up growth in young male Sprague-Dawley rats. Br J Nutr 116, 5969.
34. Bozzini, C, Champin, GM, Alippi, RM, et al. (2013) Static biomechanics in bone from growing rats exposed chronically to simulated high altitudes. High Alt Med Biol 14, 367374.
35. Arjmandi, BH, Alekel, L, Hollis, BW, et al. (1996) Dietary soybean protein prevents bone loss in an ovariectomized rat model of osteoporosis. J Nutr 126, 161.
36. Ghisolfi, J, Fantino, M, Turck, D, et al. (2013) Nutrient intakes of children aged 1–2 years as a function of milk consumption, cows’ milk or growing-up milk. Public Health Nutr 16, 524534.
37. Cai, DJ, Zhao, Y, Glasier, J, et al. (2005) Comparative effect of soy protein, soy isoflavones, and 17β‐estradiol on bone metabolism in adult ovariectomized rats. J Bone Miner Res 20, 828839.
38. Tsuchita, H, Suzuki, T & Kuwata, T (2001) The effect of casein phosphopeptides on calcium absorption from calcium-fortified milk in growing rats. Br J Nutr 85, 510.
39. Lee, YS, Noguchi, T & Naito, H (1980) Phosphopeptides and soluble calcium in the small intestine of rats given a casein diet. Br J Nutr 43, 457467.
40. Bennett, T, Desmond, A, Harrington, M, et al. (2000) The effect of high intakes of casein and casein phosphopeptide on calcium absorption in the rat. Br J Nutr 83, 673680.
41. Yuan, YV & Kitts, DD (1991) Confirmation of calcium absorption and femoral utilization in spontaneously hypertensive rats fed casein phosphopeptide supplemented diets. Nutr Res 11, 12571272.
42. Adil, S (2016) Insight into chicken egg proteins and their role in chemical defense mechanism. Int J Poult Sci 15, 7680.
43. Wagner, CL & Greer, FR (2008) Prevention of rickets and vitamin D deficiency in infants, children, and adolescents. Pediatrics 122, 11421152.
44. Bischoff-Ferrari, HA, Willett, WC, Wong, JB, et al. (2009) Prevention of nonvertebral fractures with oral vitamin D and dose dependency: a meta-analysis of randomized controlled trials. Arch Intern Med 169, 551561.
45. Jones, KS, Assar, S, Harnpanich, D, et al. (2014) 25(OH)D2half-life is shorter than 25(OH)D3half-life and is influenced by DBP concentration and genotype. J Clin Endocrinol Metab 99, 33733381.

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed