Skip to main content Accessibility help
×
Home

The effect of protein quality and fibre level in the diet and microbial activity in the digestive tract on protein utilization and energy digestibility in rats

  • B. O. Eggum (a1), R. M. Beames (a1), J. Wolstrup (a2) and K. E. Bach Knudsen (a3)

Abstract

1. Two nitrogen-balance experiments were performed with growing rats to test the effect of dietary fibre level, protein quality and antibiotic inclusion on microbial activity, N excretion patterns and energy digestibility. Each experiment involved eight dietary treatments in a 2 x 2 x 2 factorial design, with five rats per treatment. The eight treatments resulted from a combination of two protein treatments, two fibre treatments and two antibiotic treatments. In Expt 1 the protein was provided as barley, or barley plus 2 g L-lysine hydrochloride/kg dry matter (DM) (at 15 g N/kg DM) and in Expt 2 as soya-bean meal or soya-bean meal plus 2 g DL-methionine/kg DM (at 15 g N/kg DM). In both experiments the basal diet was provided with or without additional fibre as 100 g barley husk/kg DM and with or without antibiotic as 7 g Nebacitin/kg DM.

2. With both barley and soya-bean meal, true protein digestibility (TD) was improved with the addition of amino acids. Only with the soya-bean meal diets was TD increased with Nebacitin treatment, with the effect of Nebacitin and methionine being additive. Barley husk slightly reduced the TD of soya-bean meal.

3. The effect of treatments on biological value (BV) was considerable. Lysine increased BV of the barley diet from 0.741 to 0.815 whereas Nebacitin reduced BV from 0.799 to 0.757. Methionine increased the BV of soya-bean meal from 0.754 to 0.911 while BV was reduced by Nebacitin from 0.843 to 0.821 and by barley husk from 0.845 to 0.820.

4. Net protein utilization (NPU) was markedly improved by the addition of amino acids and reduced by the addition of Nebacitin. Barley husk reduced NPU with soya-bean meal diets only.

5. Amino acid addition had no effect on energy digestibility of either diet, but Nebacitin reduced this value by approximately 5% on the barley diets, with a smaller reduction on the soya-bean meal diets. Fibre reduced energy digestibility of both diets.

6. On both diets, blood urea (BU) decreased with addition of amino acids. Nebacitin increased BU with the barley diets, but decreased BU with the soya-bean meal diets. With the soya-bean meal diets, the decrease with a combination of barley husk and methionine was particularly marked.

7. Microbial activity, as measured by caecal ATP activity, was strongly reduced by antibiotic addition with both protein sources. On the soya-bean meal diets ATP activity was increased by the addition of methionine.

8. The results demonstrated a difference in response to the addition of fibre and the first limiting amino acid to barley and soya-bean meal, as measured by several indicators of protein and energy utilization.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      The effect of protein quality and fibre level in the diet and microbial activity in the digestive tract on protein utilization and energy digestibility in rats
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      The effect of protein quality and fibre level in the diet and microbial activity in the digestive tract on protein utilization and energy digestibility in rats
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      The effect of protein quality and fibre level in the diet and microbial activity in the digestive tract on protein utilization and energy digestibility in rats
      Available formats
      ×

Copyright

References

Hide All
Argenzio, R. A. (1982). In Physiologie Digestive Chez le Pore, pp. 207215 [Laplace, P.J., Corring, T., Rerat, A., editors]. Paris: INRA.
Association of Official Analytical Chemists (1975). Official Methods of Analysis, 11th ed. Washington, DC: Association of Official Analytical Chemists.
Bach Knudsen, K. E., Wolstrup, J. & Eggum, B. O. (1982). Zeitschrift für Tierphysiologie, Tierernährung und Futtermittelkunde 48, 276287.
Beames, R. M. & Eggum, B. O. (1981). British Journal of Nutrition 46, 301313.
Bjerring, J. H., Greig, M. & Halm, J. (1975). U.B.C. BMD 10V. Vancouver, British Columbia: Computing Centre of the University of British Columbia, Vancouver.
Clemens, E. T., Stevens, C. E. & Southworth, M. (1975). Journal of Nutrition 105, 759768.
Dziewiatkowski, D. D. (1970). In Sulfur in Animal Nutrition, pp. 106119. [Muth, H.O., Oldfield, J. E., editors]. Wesport: Avi.
Eggum, B. O. (1973). National Institute of Animal Science Report no. 406, pp. 173.
Eggum, B. O., Andersen, J. O. & Rotenberg, S. (1982 a). Acta Agriculturae Scandinavica 32, 145150.
Eggum, B. O. & Christensen, K. D. (1974). British Journal of Nutrition 31, 213218.
Eggum, B. O. & Chwalibog, A. (1983). Zeitschrift für Tierphysiologie, Tierernährung and Futtermittelkunde 49, 104114.
Eggum, B. O., Fekadu, M., Wolstrup, J., Sauer, W. C. & Just, A. (1979). Journal of the Science of Food and Agriculture 30, 177184.
Eggum, B. O., Thorbek, G., Beames, R. M., Chwalibog, A. & Henckel, S. (1982 b). British Journal of Nutrition 48, 161175.
Farrell, D. J. & Johnson, K. A. (1972). Animal Production 14, 209217.
Friend, D. W., Cunningham, H. M. & Nicholson, J. W. G. (1962). Canadian Journal of Animal Science 42, 5562.
Gargallo, J. & Zimmerman, D. R. (1980). Journal of Animal Science 51, 121131.
Gruhn, K. (1976). Archiv für Tierernährung 24, 8589.
Hodgdon, E. S., Horney, F. D. & Bayley, H. S. (1977). Canadian Journal of Animal Science 57, 832.
Hoque, D. E. & Elliot, J. M. (1964). Journal of Nutrition 83, 171176.

The effect of protein quality and fibre level in the diet and microbial activity in the digestive tract on protein utilization and energy digestibility in rats

  • B. O. Eggum (a1), R. M. Beames (a1), J. Wolstrup (a2) and K. E. Bach Knudsen (a3)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed