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Effect of modified dairy fat on fasting and postprandial haemostatic variables in healthy young men

  • Tine Tholstrup (a1), Peter Marckmann (a1), John Hermansen (a2), Gunhild Hølmer (a3) and Brittmarie Sandström (a1)...

Abstract

It has been suggested that milk fat, due to its content of saturated fatty acids, may have a thrombogenic effect. In the present study the fatty acid profile of milk fat was modified by changing the feeding regimens of cows and the effect on haemostatic variables of a diet containing the modified milk fat (M) was compared with that of a diet containing milk fat of typical Danish composition (D). In the modified fat 16% of the saturated fatty acid (C12–C16) content was replaced mainly by oleic acid. Eighteen subjects were fed on two strictly controlled isoenergetic diets containing 40% energy from total fat (30% energy from the test fats) for periods of 4 weeks in a study with a crossover design. Fasting samples were taken in the last week of each study period. Postprandial samples were taken on day 21, 3 h after lunch (n 18), and on the last day of the study 2, 4, 6 and 8 h after a fat load containing 1·2 g of one of the milk fats/kg body weight (n 8). After 4 weeks' dietary intervention fasting plasma factor VII coagulant (FVIIc) activity, tissue-type plasminogen activator (t-PA) activity, plasminogen activator inhibitor (PAI-1) antigen and β-thromboglobulin did not differ between diets M and D. Postprandially FVIIc and t-PA activities increased (P < 0·001) and PAI-1 antigen and PAI-1 activity decreased (P < 0·001) as compared with fasting values, regardless of diet. After the fat load, the postprandial increase in FVIIc was marginally lower after diet M than diet D (diet effect, P < 0·05). In conclusion, the modified milk fat obtained by the applied feeding strategy had virtually the same effects on haemostatic variables as conventional milk fat.

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Corresponding author

*Corresponding author: Dr Tine Tholstrup, fax +45 3528 2469, email TineTholstrup@fhe.kvl.dk

References

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Almendingen, K, Seljeflot, I, Sandstad, B & Pederson, JI (1996) Effects of partially hydrogenated fish oil, partially hydrogenated soybean oil, and butter on hemostatic variables in men. Arteriosclerosis, Thrombosis and Vascular Biology 16, 375380.
Anderson, JT, Grande, F & Keys, A (1961) Hydrogenated fats in the diet and lipids in the serum of man. Journal of Nutrition 75, 388394.
Andreotti, F, Davies, GJ, Hackett, DR, Khan, MI, De Bart, ACW, Aber, VR, Maseri, A & Kluft, C (1988) Major circadian fluctuations in fibrinolytic factors and possible relevance to time of onset of myocardial infarction, sudden cardiac death and stroke. American Journal of Cardiology 62, 635637.
Bijnen, FCH, Feskens, EJM, Giampaoli, S, Menotti, A, Fidanza, F, Hornstra, G, Caspersen, CJ, Mosterd, WL & Kromhout, D (1996) Haemostatic parameters and lifestyle factors in elderly men in Italy and The Netherlands. Thrombosis and Haemostasis 76, 411416.
Bisovsky, S, Richter, H, Fitscha, P, O'Grady, J & Sinzinger, H (1992) Postprandial hyperlipemia does not inhibit platelet aggregation. Prostaglandins Leukotrienes and Essential Fatty Acids 47, 331332.
Bladbjerg, EM, Marckmann, P, Sandström, B & Jespersen, J (1994) Non-fasting factor I coagulant activity (FVII:C) increased by high-fat diet. Thrombosis and Haemostasis 71, 755758.
Bladbjerg, EM, Tholstrup, T, Marckmann, P, Sandström, B & Jespersen, J (1995) Dietary changes in fasting levels of factor VII coagulant activity (FVII:C) accompanied by changes in factor VII protein and other vitamin K-dependent proteins. Thrombosis and Haemostasis 73, 239242.
Botti, RE & Ratnoff, OD (1963) The clot-promoting effect of soaps of long-chain saturated fatty acids. Journal of Clinical Investigation 42, 15691577.
Bysted, A, Hølmer, G & Lund, P (1998) Influence of moderate amounts of trans fatty acids on the formation of polyunsaturated fatty acids. Journal of the American Oil Chemists Society 75, 225234.
Connor, W (1962) The acceleration of thrombus formation by certain fatty acids. Journal of Clinical Investigation 41, 11991205.
Connor, WE & Poole, JCF (1961) The effect of fatty acids on the formation of thrombi. Quarterly Journal of Experimental Physiology 46, 17.
Dawes, J, Smith, RC & Pepper, DS (1978) The release, distribution, and clearance of human β-thromboglobulin and platelet factor 4. Thrombosis Research 12, 851861.
Freese, R & Mutanen, M (1995) Postprandial changes in platelet function and coagulation factors after high-fat meals with different fatty acid compositions. European Journal of Clinical Nutrition 49, 658664.
Fuhrman, B, Brook, JG & Aviram, M (1986) Increased platelet aggregation during alimentary hyperlipemia in normal and hypertriglyceridemic subjects. Annals of Nutrition and Metabolism 30, 250260.
Gram, J, Declerck, PJ, Jespersen, J & Kluft, C (1993) Multicentre evaluation of commercial kit methods: plasminogen activator inhibitor activity. Thrombosis and Haemostasis 70, 852857.
Hermansen, JE (1995) Prediction of milk fatty acid profile in dairy cows fed dietary fat differing in fatty acid composition. Journal of Dairy Science 78, 872879.
Høy, C-E & Hølmer, G (1988) Dietary linoleic acid and the fatty acid profiles in rats fed partially hydrogenated marine oils. Lipids 23, 973980.
Johnston, RV, Giner, JV, Lowe, GDO, Forbes, CD & Prentice, CRM (1982) Prevention of lipid induced platelet aggregation by aspirin. Thrombosis Research 27, 235239.
Judd, JT, Clevidence, BA, Muesing, RA, Wittes, J, Sunkin, ME & Podczasy, JJ (1994) Dietary trans fatty acids: effects on plasma lipids and lipoproteins of healthy men and women. American Journal of Clinical Nutrition 59, 861868.
Junker, R, Heinrich, J, Schulte, H, van Loo, J & Assman, G (1997) Coagulation factor VII and the risk of coronary heart disease in healthy men. Arteriosclerosis, Thrombosis and Vascular Biology 17, 15391544.
Larsen, LF, Bladbjerg, EM, Jespersen, J & Marckmann, P (1997) Effects of dietary fat quality and quantity on postprandial activation of blood coagulation factor VII. Arteriosclerosis, Thrombosis and Vascular Biology 17, 29042909.
Marckmann, P, Bladbjerg, EM & Jespersen, J (1997) Dietary fish oil (4 g daily) and cardiovascular risk markers in healthy men. Arteriosclerosis, Thrombosis and Vascular Biology 17, 33843391.
Marckmann, P, Jespersen, J, Leth, T & Sandström, B (1991) Effect of fish diet versus meat diet on blood lipids, coagulation and fibrinolysis in healthy young men. Journal of Internal Medicine 229, 317323.
Marckmann, P, Jespersen, J & Sandström, B (1992 a) Dietary n-3 and n-6 polyunsaturated fatty acids affect the fibrinolytic system differently. In Essential Fatty Acids and Eicosanoids. Invited Papers from the Third International Congress, pp. 325329 [Sinclair, A and Gibson, R, editors]. Champaign, IL: American Oil Chemists' Society.
Marckmann, P, Sandström, B & Jespersen, J (1990) Effects of total fat content and fatty acid composition in diet on factor VII coagulant activity and blood lipids. Atherosclerosis 80, 227233.
Marckmann, P, Sandström, B & Jespersen, J (1992 b) Fasting blood coagulation and fibrinolysis of young adults unchanged by reduction in dietary fat content. Arteriosclerosis and Thrombosis 12, 201205.
Marckmann, P, Sandström, B & Jespersen, J (1993 a) Dietary effects on circadian fluctuation in human blood coagulation factor VII and fibrinolysis. Atherosclerosis 101, 225234.
Marckmann, P, Sandström, B & Jespersen, J (1993 b) Favorable long-term effect of a low-fat/high-fiber diet on human blood coagulation and fibrinolysis. Arteriosclerosis and Thrombosis 13, 505511.
Marckmann, P, Sandström, B & Jespersen, J (1994) Low-fat high-fiber diet favorably affects several independent risk markers of ischemic heart disease. Observations on blood lipids, coagulation, and fibrinolysis from a trial of middle-aged Danes. American Journal of Clinical Nutrition 59, 935939.
Meade, TW, Ruddock, V, Stirling, Y, Chakrabarti, R & Miller, GJ (1993) Fibrinolytic activity, clotting factors, and long-term incidence of ischaemic heart disease in the Northwick Park Heart Study. Lancet 342, 10761079.
Mensink, RP & Katan, MB (1990) Effect of dietary trans fatty acids on high-density and low-density lipoprotein cholesterol levels in healthy subjects. New England Journal of Medicine 323, 439445.
Miller, GJ, Martin, JC, Mitropoulos, KA, Esnouf, MP, Cooper, JA, Morrissey, JH, Howarth, DJ & Tuddenham, EGD (1996) Activation of factor VII during alimentary lipemia occurs in healthy adults and patients with congenital factor XII or factor XI deficiency, but not in patients with factor IX deficiency. Blood 87, 41874196.
Mutanen, M & Aro, A (1997) Coagulation and fibrinolysis factors in healthy subjects consuming high stearic or trans fatty acid diets. Thrombosis and Haemostasis 77, 99104.
Nimpf, J, Malle, E, Leopold, B, Wurm, H & Kostner, GM (1989) Postprandial hyperlipemia inhibits platelet aggregation without affecting prostanoid metabolism. Prostaglandins Leukotrienes and Essential Fatty Acids 37, 713.
Nordøy, A & Goodnight, SH (1990) Dietary lipids and thrombosis. Relationships to atherosclerosis. Atherosclerosis 10, 149163.
Salomaa, V, Rasi, V, Pekkanen, J, Jauhiainen, M, Vahtera, E, Pietinen, P, Korhonen, H, Kuulasmaa, K & Ehnholm, C (1993) The effects of saturated fat and n-6 polyunsaturated fat on postprandial lipemia and hemostatic activity. Atherosclerosis 103, 111.
Sanders, TAB, Oakley, FR, Miller, GJ, Mitropoulos, KA, Crook, D & Oliver, MF (1997) Influence of n-6 versus n-3 polyunsaturated fatty acids in diets low in saturated fatty acids on plasma lipoproteins and hemostatic factors. Arteriosclerosis, Thrombosis and Vascular Biology 17, 34493460.
Silveira, A, Karpe, F, Blombäck, M, Steiner, G, Walldius, G & Hamsten, A (1994) Activation of coagulation factor VII during alimentary lipemia. Arteriosclerosis and Thrombosis 14, 6069.
Silveira, A, Karpe, F, Johnsson, H, Bauer, KA & Hamsten, A (1996) In vivo demonstration in humans that large postprandial triglyceride-rich lipoproteins activate coagulation factor VII through the intrinsic coagulation pathway. Arteriosclerosis, Thrombosis and Vascular Biology 16, 13331339.
Smith, EB (1986) Fibrinogen, fibrin and fibrin degradation products in relation to atherosclerosis. Clinical Haematology 15, 355370.
Takahashi, H, Yoshino, N & Shibata, A (1988) Measurement of platelet factor 4 and β-thromboglobulin by an enzyme-linked immunosorbent assay (letter). Clinica Chimica Acta 175, 113114.
Tholstrup, T, Andreasen, K & Sandström, B (1996) Acute effect of high-fat meals rich in either stearic or myristic acid on hemostatic factors in healthy young men. American Journal of Clinical Nutrition 64, 168176.
Tholstrup, T, Marckmann, P, Jespersen, J & Sandström, B (1994 a) Fat high in stearic acid favorably affects blood lipids and factor VII coagulant activity in comparison with fats high in palmitic acid or high in myristic and lauric acids. American Journal of Clinical Nutrition 59, 371377.
Tholstrup, T, Marckmann, P, Jespersen, J, Vessby, B Jart Aa & Sandström, B (1994 b) Effect on blood lipids, coagulation and fibrinolysis of a fat high in myristic acid and a fat high in palmitic acid. American Journal of Clinical Nutrition 60, 919925.
Tholstrup, T, Sandström, B, Hermansen, JE & Hølmer, G (1998) Effect of modified dairy fat on postprandial and fasting plasma lipids and lipoproteins in healthy young men. Lipids 33, 1121.
Thomson, JM (editor) (1985) Blood Coagulation and Haemostasis, A Practical Guide, 4th ed. New York: Churchill Livingstone.
Wood, R, Kubena, K, O'Brien, B, Tseng, S & Martin, G (1993) Effect of butter, mono- and polyunsaturated fatty acid-enriched butter, trans fatty acid margarine, and zero trans fatty acid margarine on serum lipids and lipoproteins in healthy men. Journal of Lipid Research 34, 111.
World Health Organization (1985) Energy and Protein Requirements. Technical Report Series no. 724. Geneva: WHO.

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