Hostname: page-component-84b7d79bbc-l82ql Total loading time: 0 Render date: 2024-07-25T18:17:09.171Z Has data issue: false hasContentIssue false
  • English
  • Français

Distribution of α-tocopherol stereoisomers in liver and thigh of chickens

Published online by Cambridge University Press:  09 March 2007

Lucia Cortinas ,
Ana Barroeta ,
Jaume Galobart  and
Søren K. Jensen
Lucia Cortinas
Affiliation:
Department of Animal and Food Science, Facultat de Veterinària, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain
Ana Barroeta*
Affiliation:
Department of Animal and Food Science, Facultat de Veterinària, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain
Jaume Galobart
Affiliation:
Department of Animal and Food Science, Facultat de Veterinària, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain
Søren K. Jensen
Affiliation:
Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK-8830 Tjele, Denmark
*
*Corresponding author: Dr Ana Barroeta, fax +34 93 581 14 94, email ana.barroeta@uab.es
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effect of supplementation with different levels of all-rac-α-tocopheryl acetate and the inclusion of different dietary contents of PUFA on the deposition of α-tocopherol stereoisomers in liver and thigh of chickens was evaluated. Ninety-six 1-d-old Ross female broiler chickens were randomly distributed into eight experimental treatments (three replicates each) resulting from four levels of α-tocopheryl acetate without supplementation and supplemented with 100, 200 and 400 mg α-tocopheryl acetate/kg and two levels of dietary PUFA (15 and 61 g/kg). The feeds supplemented with α-tocopheryl acetate contained a similar proportion of each stereoisomer. The diets without α-tocopheryl acetate had the following α-tocopherol stereoisomers (%): RRR 35·1, RRS 24·5, RSR 25·3, RSS 13·9 and total 2S forms 1·3. Consumption of different levels of α-tocopheryl acetate did not lead to statistical differences in α-tocopherol stereoisomer proportion in the liver and thigh. In general, the stereoisomer profiles in the tissues studied were similar, responding to the stereoisomer profile of the diet. Both tissues preferentially accumulated 2R stereoisomer (69–100%). However, when α-tocopheryl acetate was used the discrimination was not specific for the RRR α-tocopherol form. Furthermore, the 2R:2S ratio had a tendency to increase as the polyunsaturation level of the diet increased.

Keywords

α-Tocopherol stereoisomersDietary polyunsaturationThighLiverChicken
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 2 , August 2004 , pp. 295 - 301
Copyright
Copyright © The Nutrition Society 2004

References

Atteh, JO & Leeson, SResponse of laying hens diets to dietary saturated and unsaturated fatty acids in the presence of varying dietary calcium levels. Poult Sci (1985) 64, 520–528.CrossRefGoogle ScholarPubMed
Burton, GW, Ingold, KU, Zahalka, H, Dutton, P, Hodgkinson, B, Hughes, L, Foster, DO & Behrens, WABiodiscrimination of tocopherols In Vitamin E. Its Usefulness in Health and in Curing Diseases. pp. 51–61. [M, Mino, H, Nakamura, A, Diplock & HJ, Kayden editors]. Tokyo: Japan Scientific Societies Press. (1993)Google Scholar
Cortinas, L, Barroeta, AC, Villaverde, C, Baucells, MD & Jensen, SKInteraction of dietary unsaturation level with linolenic acid and a-tocopherol deposition in poultry meat In Proceedings of the Joint Nutrition Symposium pp. 133–134Antwerp: European Society of Veterinary Nutrition. (2002)Google Scholar
Cortinas, L, Galobart, J, Barroeta, AC, Castillo, MS & Jensen, SKInfluencia del nivel de insaturación dietética sobre el depósito y efecto antioxidante del alfa-tocoferol en muslo de pollo (crudo, cocido y cocido–refrigerado) (Influence of the level of dietary unsaturation on deposit and antioxidant effect of alpha-tocopherol in chicken thigh meat (raw, cooked and refrigerated-cooked)) In Proceedings of the XXXVIII Symposium Científico de Avicultura. Sección Española de la WPSA. pp. 141–148Cordoba: Sección Española de La Asociación Mundial de Avicultura Cientófica. (2001)Google Scholar
Drotleff, AM & Ternes, WDetermination of RS, E/Z-tocotrienols by HPLC. J Chromatogr A (2001) 909. 215–223.CrossRefGoogle ScholarPubMed
Dutta-Roy, AK, Gordon, MJ, Campbell, FM, Duthie, GG & James, WPTVitamin E requirements, transport, and metabolism: role of α–tocopherol-binding proteins. J Nutr Biochem (1994) 5. 562–570.CrossRefGoogle Scholar
Flachowsky, G, Engelman, D, Sünder, A, Halle, I & Sallmann, HPEggs and poultry meat as tocopherol sources in dependence on tocopherol supplementation of poultry diets. Food Res Int (2002) 35. 239–243.CrossRefGoogle Scholar
Grau, A, Guardiola, F, Grimpa, S, Barroeta, AC & Codony, ROxidative stability of dark chicken meat through frozen storage: Influence of dietary fat and a-tocopherol and ascorbic acid supplementation. Poult Sci (2001) 80. 1630–1642.CrossRefGoogle Scholar
Hosomi, A, Arita, M, Sato, Y, Kiyose, C, Ueda, T, Igarashi, O, Arai, H & Inoue, KAffinity for a-tocopherol transfer protein as a determinant of the biological activities of vitamin E analogs. FEBS Lett (1997) 409. 105–108.Google Scholar
Husveth, F, Manilla, HA, Gaal, T, Vajdourch, P, Balogh, N, Wagner, L, Loth, I & Nemeth, KEffects of saturated and unsaturated fats with vitamin E supplementation on the antioxidant status of broiler tissues. Acta Vet Hung (2000) 48. 69–79.Google Scholar
Ingold, KU, Burton, GW, Foster, DO & Hughes, LIsmethyl-branching in atocopherol’s “tail” important for its in vivo activity? Rat curative myopathy bioassay measurements of the vitamin E activity of three 2RS–n–alkyl–2,5,7,8–tetramethyl–6–hydroxychromans. Free Radic Biol Med (1990) 9. 205–210.CrossRefGoogle Scholar
Jensen, SK, Engberg, RM & Hedermann, MSAll-rac-αtocopherol acetate is a better vitamin E source than all-racα-α-tocopherol succinate for broilers. J Nutr (1999) 129. 1355–1360.CrossRefGoogle ScholarPubMed
Kaneko, K, Kiyose, C, Ueda, T, Ichikawa, H & Igarashi, OStudies of the metabolism of α–tocopherol stereoisomers in rats using [5–methyl–14C]SRR– and RRR–α–tocopherol. Lipid Res (2000) 41. 357–367.CrossRefGoogle Scholar
Kiyose, C, Muramatsu, R, Ueda, T& Igarashi, OChange in the distribution of α–tocopherol stereoisomers in rats after intravenous administration. Biosci Biotech Biochem (1995) 59. 791–795.CrossRefGoogle ScholarPubMed
Kuhlenkamp, J, Ronk, M, Yusin, M, Stolz, A & Kaplowitz, NIdentification and purification of a human liver cytosolic tocopherol binding protein. Protein Expr Purif (1993) 4. 382–389.CrossRefGoogle ScholarPubMed
Lauridsen, C, Engel, H, Jensen, SK, Craig, AM & Traber, MGLactating sows and suckling preferentially incorporate RRR- over all–rac–α–tocopherol into milk, plasma and tissues. J Nutr (2002) 132. 1258–1264.CrossRefGoogle ScholarPubMed
Leonard, SW, Terasawa, Y, Farese, RV Jr & Traber, MGIncorporation of deuterated RRR– or all–rac–α–tocopherol in plasma and tissues of α–tocopherol transfer protein–null mice. Am J Clin Nutr (2002) 75. 555–560.CrossRefGoogle ScholarPubMed
Machlin, LJ, Gabriel, E & Brin, MBiopotency of α–tocopherols as determined by curative myopathy bioassay in the rat. J Nutr (1982) 112. 1437–1440.CrossRefGoogle ScholarPubMed
Muggli, RPhysiological requirements of vitamin E as a function of the amount and type of polyunsaturated fatty acid. World Rev Nutr Diet (1994) 75. 166–168.CrossRefGoogle ScholarPubMed
National Research Council Nutrient Requirement for Poultry, 9th ed. Washington, DC: National Academy Press. (1994)Google Scholar
Nitta, C, Hayashi, K, Ueda, T & Igarashi, ODistribution of α–tocopherol stereoisomers in rats. Biosci Biotech Biochem (1993) 57. 1406–1407.CrossRefGoogle Scholar
Piironen, VI, Liljeroos, AI & Koivistoinen, PETransfer of α–tocopherol stereoisomers from feeds to eggs. J Agric Food Chem (1991) 39. 99–101.CrossRefGoogle Scholar
Riss, G, Kormann, AW, Glinz, E, Walther, W & Ranalder, UBSeparation of the eight stereoisomers of all–rac–α–tocopherol from tissues and plasma: chiral phase high-performance liquid chromatography and capillary gas chromatography. Methods Enzymol (1994) 234. 302–310.CrossRefGoogle ScholarPubMed
Sato, Y, Arai, H, Miyata, A, Tokita, S, Yamamoto, K, Tanabe, T & Inoue, KPrimary structure of α–tocopherol transfer protein from rat liver. J Biol Chem (1993) 268. 17705–17710.Google Scholar
Sato, Y, Hagiwara, K, Arai, H & Inoue, KPurification and characterization of the α–tocopherol transfer protein from rat liver. FEBS Lett (1991) 188. 41–45.Google Scholar
Scaife, JR, Moyo, J, Galbraith, H, Michie, W & Campbell, VEffect of different dietary supplemental fats and oils on the tissue fatty acid composition and growth of female broilers. Br Poult Sci (1994) 35. 107–118.CrossRefGoogle ScholarPubMed
Scott, CG, Cohen, N, Riggio, PP, Gas chromatographic assay of the diastediastereomeric composition of all–rac–α–tocopheryl acetate. Lipids (1982) 17. 97–101.Google Scholar
Traber, MG, Burton, GW, Ingold, KU & Kayden, HJRRRand SRR–α–tocopherols are secreted without discrimination in human chylomicrons, but RRR–α–tocopherol is preferentially secreted in very low density lipoproteins. J Lipid Res (1990) 31. 675–685.CrossRefGoogle ScholarPubMed
Ueda, T, Ichikawa, H & Igarashi, Oα–tocopherols stereoisomers in biological specimens chiral phase high–performance liquid chromatography. Sci Vitaminol (1993) 39. 207–219.Google Scholar
Weiser, H, Riss, G & Kormann, AWBiodiscrimination of the eight α–tocopherol stereoisomers results in preferential accumulation of the four 2R forms in tissues and plasma of rats. J Nutr (1996) 126. 2539–2549.CrossRefGoogle ScholarPubMed
Weiser, H & Vecchi, MStereoisomers of α–tocopheryl acetate. II. Biopotencies of all eight stereoisomers, individually or in mixtures, as determined by rat resorption-gestation tests.. Int J Vit Nutr Res (1982) 52. 351–370.Google ScholarPubMed