Skip to main content Accessibility help
×
Home

Dissimilation of 1,2-propanediol by rumen micro-organisms

  • J. W. Czerkawski (a1) and Grace Breckenridge (a1)

Abstract

1. The main products of fermentation of 1,2-propanediol were n-propanol and propionic acid, but variable amounts of acetic acid and carbon dioxide were also formed. The concentrations of an intermediate propionaldehyde increased and then decreased.

2. A tentative scheme is suggested, showing that 1,2-propanediol is first dehydrated to propionaldehyde, which is then reduced to n-propanol. The scheme also explains the formation of propionic and acetic acids and shows how the metabolism of 1,2-propanediol is related to that of rhamnose.

3. Experiments with samples of rumen contents from animals on various diets showed that 1,2-propanediol was metabolized most rapidly when the animals were given molassed sugar-beet pulp. The rates of dissimilation of the diol increased with the concentration of rumen contents and with the concentration of substrate.

4. The dissimilation of 1,2-propanediol by rumen micro-organisms resulted in an increased uptake of hydrogen. The metabolic hydrogen, arising from the inhibition of methane production by chloroform, appeared to be better utilized than the gaseous hydrogen.

5. Oxygen gas did not affect the utilization of 1,2-propanediol, but the diol increased the uptake of oxygen by the rumen contents. The hydrogen and carbon balances were better when 1,2-propanediol was incubated anaerobically than in the presence of oxygen.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Dissimilation of 1,2-propanediol by rumen micro-organisms
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Dissimilation of 1,2-propanediol by rumen micro-organisms
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Dissimilation of 1,2-propanediol by rumen micro-organisms
      Available formats
      ×

Copyright

References

Hide All
Abeles, R. H. & Lee, H. A. Jr (1961). J. biol. Chem. 236, 2347.
Brownstein, A. M. & Abeles, R. H. (1961). J. biol. Chem. 236, 1199.
Clapperton, J. L. & Czerkawski, J. W. (1972 a). Br. J. Nutr. 27, 553.
Clapperton, J. L. & Czerkawski, J. W. (1972 b). Proc. Nutr. Soc. 31, 55A.
Conway, E. J. (1962). Microdiffusion Analysis and Volumetric Error, p. 234. London: Crosby, Lockwood and Son.
Cottyn, B. G. & Boucque, C. V. (1968). J. agric. Fd Chem. 16, 105.
Czerkawski, J. W. (1966). Br. J. Nutr. 20, 833.
Czerkawski, J. W. & Breckenridge, G. (1969). Br. J. Nutr. 23, 51.
Czerkawski, J. W. & Breckenridge, C. (1970). Lab. Pract. 19, 717.
Czerkawski, J. W. & Breckenridgr, G. (1971). Lab. Pract. 20, 403.
Czerkawski, J. W. & Breckenridge, G. (1972). Br. J. Nutr. 27, 131.
Czerkawski, J. W. & Clapperton, J. L. (1968). Lab. Pract. 17, 994.
Emery, R. S., Burg, N., Brown, L. D. & Blank, G. N. (1964). J. Dairy Sci. 47, 1074.
Emery, R. S., Brown, R. E. & Black, A. L. (1967). J. Nutrition 92, 348.
Huff, E. (1959). Analyt. Chem. 31, 1626.
Huff, E. & Rudney, H. (1959). J. biol. Chem. 234, 1060.
Jones, L. R. & Riddick, J. A. (1957). Analyt. Chem. 29, 1214.
Kluyver, A. J. & Schnellen, C. (1937). Enzymologia 4, 7.
Ladd, J. N. & Walker, D. J. (1959). Biochem. J. 71, 365.
McDougall, E. I. (1948). Biochem. J. 43, 99.
Noble, R. C. & Czerkawski, J. W. (1972). Analyst (in the Press).
Rudney, H. (1950). Arch Biochem. 29, 231.
Rudney, H. (1954). J. biol. Chem. 210, 361.
Shull, K. H. & Miller, O. N. (1960). J. biol. Chem. 235, 551.
Ting, S.-M., Sellinger, O. Z. & Miller, O. N. (1964). Biochim. biophys. Acta 89, 217.
Toraya, T., Sugimoto, Y., Tamao, Y., Shimizu, S. & Fukui, F. (1971). Biochemistry, N. Y. 10, 3475.
Vogel, A. I. (1951). Practical Organic Chemistry. London, New York and Toronto: Longman, Green and Co.
Waldo, D. R. & Schultz, L. H. (1960). J. Dairy Sci. 43, 496.

Dissimilation of 1,2-propanediol by rumen micro-organisms

  • J. W. Czerkawski (a1) and Grace Breckenridge (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.