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Dietary fructo-oligosaccharides in healthy adults do not negatively affect faecal cytotoxicity: a randomised, double-blind, placebo-controlled crossover trial

  • Petra A. M. J. Scholtens (a1), Martine S. Alles (a1), Linette E. M. Willemsen (a2), Claudia van den Braak (a2), Jacques G. Bindels (a1), Günther Boehm (a1) and Mirjam J. A. P. Govers (a2)...

Abstract

Fructo-oligosaccharides (FOS) are widely used in commercial food products. Most studies on FOS concern the health benefits, but some negative effects were recently reported concerning thefaecal cytotoxicity and excretion of mucin-type oligosaccharides in combination with a Ca-restricted diet. The present study was performed to investigate whether these effects of FOS are observed in adults consuming a regular diet unrestricted in Ca. The study was a randomised, double-blind, placebo-controlled crossover trial, involving eleven healthy adults, who consumed 25–30g FOS or maltodextrin (control) in a random order for 2 weeks in addition to their regular diet. Stools were collected for analysis of pH and SCFA (as markers of fermentation), for the assessment of faecal water cytotoxicity, and for the analysis of alkaline phosphataseactivity (as a marker of epithelial cell turnover) andO-linked oligosaccharides (to estimate the excretion of mucin-type oligosaccharides). FOS consumption significantly altered bacterial fermentation (increased percentage of acetate, decreased percentage of butyrate) and tended to decrease stool pH. Furthermore, FOS consumption resulted in a significantly higher stool frequency and in significantly more complaints of flatulence. No significant differences between the control and FOS period were observed in the mean cytotoxicity of faecal water (37·5 (sem 6·9) % v. 18·5 (sem 6·9) % P=0·084), in mean alkaline phosphatase activity (27·7 (sem 2·9) v. 24·6 (sem 3·2) U/g dry faeces; P=0·496) or in the mean excretion of mucin-type oligosaccharides (49·9 (sem 4·0)v. 53·5 (sem 4·3) mg/g dry faeces; P=0·553). We conclude that dietary FOS in a dose up to 25–30g/d altered the bacterial fermentation pattern but did not affect faecal cytotoxicity or the faecal concentration of mucin-type oligosaccharides in human adults consuming a regular diet.

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Corresponding author

*Corresponding author: Petra A. M. J. Scholtens, fax +31 317 466500, email petra.scholtens@numico-research.nl

References

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Bouhnik, Y, Flourie, B, Riottot, M, Bisetti, N, Gailing, MF, Guibert, A, Bornet, F & Rambaud, JCEffects of fructo-oligosaccharides ingestion on fecal bifidobacteria and selected metabolic indexes of colon carcinogenesis in healthy humans. Nutr Cancer 1996 26, 2129.
Bouhnik, Y, Vahedi, K, Achour, L, Attar, A, Salfati, J, Pochart, P, Marteau, P, Flourie, B, Bornet, F & Rambaud, JCShort-chain fructo-oligosaccharide administration dose-dependently increases fecal bifidobacteria in healthy humans. J Nutr 1999 129, 113116.
Bovee-Oudenhoven, IM, ten Bruggencate, SJ, Lettink-Wissink, ML & van der Meer, RDietary fructo-oligosaccharides and lactulose inhibit intestinal colonisation but stimulate translocation of salmonella in rats. Gut 2003 52, 15721578.
Bovee-Oudenhoven, I, Termont, D, Dekker, R & van der Meer, RCalcium in milk and fermentation by yoghurt bacteria increase the resistance of rats to Salmonella infection. Gut 1996 38, 5965.
Buddington, RK, Williams, CH, Chen, SC & Witherly, SADietary supplement of neosugar alters the fecal flora and decrease activities of some reductive enzymes in human subjects. Am J Clin Nutr 1996 63, 709716.
Crowther, RS & Wetmore, RFFluorometric assay of O-linked glycoproteins by reaction with 2-cyanoacetamide. Anal Biochem 1987 163, 170174.
Deplancke, B & Gaskins, HRMicrobial modulation of innate defense: goblet cells and the intestinal mucus layer. Am J Clin Nutr 2001 73, S1131S1141.
Finnie, IA, Dwarakanath, AD, Taylor, BA & Rhodes, JMColonic mucin synthesis is increased by sodium butyrate. Gut 1995 36, 9399.
Gibson, GR, Beatty, ER, Wang, X & Cummings, JHSelective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 1995 108, 975982.
Govers, MJ, Termont, DS, Lapre, JA, Kleibeuker, JH, Vonk, RJ & Van der Meer, RCalcium in milk products precipitates intestinal fatty acids and secondary bile acids and thus inhibits colonic cytotoxicity in humans. Cancer Res 1996 56, 32703275.
Govers, MJ, Termont, DS & Van der Meer, RMechanism of the antiproliferative effect of milk mineral and other calcium supplements on colonic epithelium. Cancer Res 1994 54, 95100.
Govers, MJ & Van der Meer, REffects of dietary calcium and phosphate on the intestinal interactions between calcium, phosphate, fatty acids, and bile acids. Gut 1993 34, 365370.
Hoskins, LCHuman enteric population ecology and degradation of gut mucins Dig Dis Sci 1981 26, 769772.
Hoskins, LC, Agustines, M, McKee, WB, Boulding, ET, Kriaris, M & Niedermeyer, GMucin degradation in human colon ecosystems.Isolation and properties of fecal strains that degrade ABH blood group antigens and oligosaccharides from mucin glycoproteins. J Clin Invest 1985 75, 944953.
Jones, B & Kenward, M 2003 Design and Analysis of Cross-overn Trials, 2nded., Boca Raton, Chapman & Hall/crc. FL:
Kleessen, B, Sykura, B, Zunft, HJ & Blaut, MEffects of inulin and lactose on fecal microflora, microbial activity, and bowel habit in elderly constipated persons. Am J Clin Nutr 1997 65, 13971402.
Knol, J, Scholtens, PAMJ, Kafka, Cet al.. Colon microflora in infants fed formula with galacto- and fructo-oligosaccharides: more like breast-fed infants. J Pediatr Gastroenterol Nutr 2005 40, 3642.
Kolida, S, Tuohy, K & Gibson, GRPrebiotic effects of inulin and oligofructose. Br J Nutr 2002 87, S193S197.
Lapre, JA, De Vries, HT, Koeman, JH & Van der Meer, RThe antiproliferative effect of dietary calcium on colonic epithelium is mediated by luminal surfactants and dependent on the type of dietary fat. Cancer Res 1993 53, 784789.
Lapre, JAKleibeuker, J & van der Meer, RIntestinal alkaline phosphatase in fecal water reflects epitheliolysis and is decreased by dietary calcium. Gastroenterology 1991 100, A378.
McNeil, PL & Ito, SGastrointestinal cell plasma membrane wounding and resealing in vivo. Gastroenterology 1989 96, 12381248.
Ouwehand, AC, Grasten, S, Niemi, P, Mykkanen, H & Salminen, SWheat or rye supplemented diets do not affect faecal mucus concentration or the adhesion of probiotic micro-organisms to faecal mucus. Lett Appl Microbiol 2000 31, 3033.
Rafter, JJ, Child, P, Anderson, AM, Alder, R, Eng, V & Bruce, WRCellular toxicity of fecal water depends on diet. Am J Clin Nutr 1987 45, 559563.
Sakata, T & Setoyama, HLocal stimulatory effect of short-chain fatty acids on the mucus release from the hindgut mucosa of rats (Rattus norvegicus). Comp Biochem Physiol A Physiol 1995 111, 429432.
Shimotoyodome, A, Meguro, S, Hase, T, Tokimitsu, I & Sakata, TShort chain fatty acids but not lactate or succinate stimulate mucus release in the rat colon. Comp Biochem Physiol A Mol Integr Physiol 2000 125, 525531.
Strous, GJ & Dekker, JMucin-type glycoproteins. Crit Rev Biochem Mol Biol 1992 27, 5792.
Ten, Bruggencate SJ, Bovee-Oudenhoven, IM, Lettink-Wissink, ML, Katan, MB & Van Der Meer, RDietary fructo-oligosaccharides and inulin decrease resistance of rats to salmonella: protective role of calcium. Gut 2004a 53 530535.
Ten, Bruggencate SJM, Bovee-Oudenhoven, IM, Lettink-Wissink, ML, Katan, MB & van der meer, RDietary fructo-oligosaccharides and the intestinal barrier in humans PhD Thesis, The NetherlandsWageningen University 2004b
Ten, Bruggencate SJ, Bovee-Oudenhoven, IM, Lettink-Wissink, ML & Van der Meer, RDietary fructo-oligosaccharides dosedependently increase translocation of salmonella in rats. J Nutr 2003 133 23132318.
Tuohy, KM, Kolida, S, Lustenberger, AM & Gibson, GRThe prebiotic effects of biscuits containing partially hydrolysed guar gum and fructo-oligosaccharides – a human volunteer study. Br J Nutr 2001 86 341348.
Van Loo, JOn the presence of inulin and oligofructose as natural ingredients in the Western diet. Crit Rev Food Sci Nutr 1995 36 525552.
Willemsen, LE, Koetsier, MA, van Deventer, SJ & van Tol, EAShort chain fatty acids stimulate epithelial mucin 2 expression through differential effects on prostaglandin E(1) and E(2) production by intestinal myofibroblasts. Gut 2003 52 14421447.

Keywords

Dietary fructo-oligosaccharides in healthy adults do not negatively affect faecal cytotoxicity: a randomised, double-blind, placebo-controlled crossover trial

  • Petra A. M. J. Scholtens (a1), Martine S. Alles (a1), Linette E. M. Willemsen (a2), Claudia van den Braak (a2), Jacques G. Bindels (a1), Günther Boehm (a1) and Mirjam J. A. P. Govers (a2)...

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