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Carotene-cleavage activity in chick intestinal mucosa cytosol: association with a high-molecular-weight lipid-protein aggregate fraction and partial characterization of the activity

Published online by Cambridge University Press:  09 March 2007

David Sklan
David Sklan
Affiliation:
Faculty of Agriculture, The Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
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Abstract

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1. A fluorescent high-molecular weight lipid–protein aggregate was isolated from the cytosol of chick intestinal mucosa or liver by gel filtration on columns of Sepharose 4B or 6B.

2. This aggregate exhibited carotene-cleavage activity.

3. On incubation of this aggregate, dissociation occurred and low-molecular weight fractions containing Cu and Zn and exhibiting carotene-cleavage activity were found. This fraction appeared on sodium dodecyl sulphate polyacrylamide electrophoresis to have a molecular weight of 7000–11000 and resembled the previously described Cu chelatins in amino acid composition.

4. Carotene cleavage may be effected by a copper–zinc metalloprotein of low-molecular weight, associated in intestinal cytosol with a lipid–protein aggregate.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 50 , Issue 2 , September 1983 , pp. 417 - 425
Copyright
Copyright © The Nutrition Society 1983

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