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β-Carotene and α-tocopherol concentration and antioxidant status in buccal mucosal cells and plasma after oral supplementation

Published online by Cambridge University Press:  09 March 2007

Jürgen G. Erhardt ,
Heidrun Mack ,
Ulrike Sobeck  and
Hans K. Biesalski
Jürgen G. Erhardt*
Affiliation:
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Fruwirthstrasse 12, 70599 Stuttgart, Germany
Heidrun Mack
Affiliation:
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Fruwirthstrasse 12, 70599 Stuttgart, Germany
Ulrike Sobeck
Affiliation:
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Fruwirthstrasse 12, 70599 Stuttgart, Germany
Hans K. Biesalski
Affiliation:
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Fruwirthstrasse 12, 70599 Stuttgart, Germany
*
*Corresponding author: Dr Jürgen G. Erhardt, fax +49 711 4593822, email erhardtj@uni-hohenheim.de
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Abstract

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The uptake of α-tocopherol and β-carotene and their antioxidative effect in plasma and buccal mucosal cells after oral application in twelve subjects is demonstrated in our study. The effect on the antioxidative status was evaluated using a modified thiobarbituric acid-reactive substance (TBARS) method. As expected, the supplement of 134·2 mg α-tocopherol/d and 25 mg β-carotene/d for 7 d resulted in a significant increase of α-tocopherol and β-carotene concentration in plasma (P<0·05). In buccal mucosal cells, the concentration of β-carotene increased after supplementation (P<0·05), whereas the concentration of α-tocopherol remained constant. A decrease in TBARS (P<0·05) was found in buccal mucosal cells but not in plasma. In conclusion, an uptake of the supplemented antioxidants was detected in plasma and in buccal mucosal cells. There was significant change in β-carotene concentration and oxidative stress as measured using a modified TBARS test in buccal mucosal cells, but not in the plasma.

Keywords

Buccal mucosal cellsSupplementation-Tocopherol-CaroteneThiobarbituric acid-reactive substances
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 5 , May 2002 , pp. 471 - 475
Copyright
Copyright © The Nutrition Society 2002

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