Skip to main content Accessibility help
×
Home

Variations in the abundance of 24 protein biomarkers of beef tenderness according to muscle and animal type

  • N. Guillemin (a1), C. Jurie (a1), I. Cassar-Malek (a1), J.-F. Hocquette (a1), G. Renand (a2) and B. Picard (a1)...

Abstract

Some proteins have been revealed as biomarkers for beef tenderness by previous studies. These markers could be used in immunological tests to predict beef tenderness, in living animals as well as in carcasses. It is well known that rearing practices modify the amounts of mRNA and proteins. Therefore, the reliability of protein tests could be affected by livestock and biological effects such as production systems, breed, muscle and animal type. This study analysed the effects of animal and muscle type on 24 proteins. The animals studied were 67 young bulls and 44 steers of the Charolais breed, and muscles were Longissimus thoracis and Semitendinosus. Protein amounts were determined by Dot blot, an immunological technique. Results showed that expressions of 20 proteins were influenced by animal and/or muscle type. These results could lead to modifications and adaptations of prediction tests according to rearing practice, bovine breed and beef cut.

Copyright

Corresponding author

References

Hide All
Bernard, C, Cassar-Malek, I, LeCunff, M, Dubroeucq, H, Renand, G, Hocquette, JF 2007. New indicators of beef sensory quality revealed by expression of specific genes. Journal of Agricultural and Food Chemistry 55, 52295237.
Bouley, J 2004. Analyse protéomique du muscle squelettique bovin. PhD thesis, Université de Clermont-Ferrand 2, Clermont-Ferrand, France.
Bouley, J, Chambon, C, Picard, B 2004. Mapping of bovine skeletal muscle proteins using two-dimensional gel electrophoresis and mass spectrometry. Proteomics 4, 18111824.
Bradford, MM 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72, 248254.
Brandstetter, AM, Picard, B, Geay, Y 1998. Muscle fibre characteristics in four muscles of growing male cattle II. Effect of castration and feeding level. Livestock Production Science 53, 2536.
Brandstetter, AM, Sauerwein, H, Veerkamp, JH, Geay, Y, Hocquette, JF 2002. Effects of muscle type, castration, age and growth rate on H-FABP expression in bovine skeletal muscle. Livestock Production Science 75, 199208.
Cassar-Malek, I, Picard, B, Bernard, C, Hocquette, JF 2008. Application of gene expression studies in livestock production systems: a European perspective. Australian Journal of Experimental Agriculture 48, 701710.
Cassar-Malek, I, Jurie, C, Bernard, C, Barnola, I, Micol, D, Hocquette, JF 2009. Pasture-feeding of Charolais steers influences skeletal muscle metabolism and gene expression. Journal of Physiology and Pharmacology 60, 8390.
Cassar-Malek, I, Guillemin, N, Hocquette, JF, Micol, D, Bauchart, D, Picard, B 2010. Expression of DNAJA1 in bovine muscles according to developmental age and management factors. Animal (in press).
Choi, YM, Kim, BC 2009. Muscle fiber characteristics, myofibrillar protein isoforms, and meat quality. Livestock Science 122, 105118.
Clark, KA, McElhinny, AS, Beckerle, MC, Gregorio, CC 2002. Striated muscle cytoarchitecture: an intricate web of form and function. Annual Review of Cell and Developmental Biology 18, 637706.
Dransfield, E, Martin, JF, Bauchart, D, Abouelkaram, S, Lepetit, J, Culioli, J, Jurie, C, Picard, B 2003. Meat quality and composition of three muscles from French cull cows and young bulls. Animal Science 76, 387399.
Fink, AL 1999. Chaperone-mediated protein folding. Physiological Reviews 79, 425449.
Fritsche, S, Steinhart, H 1998. Differences in natural steroid hormone patterns of beef from bulls and steers. Journal of Animal Science 76, 16211625.
Geay, Y, Bauchart, D, Hocquette, JF, Culioli, J 2001. Effect of nutritional factors on biochemical, structural and metabolic characteristics of muscles in ruminants, consequences on dietetic value and sensorial qualities of meat. Reproduction Nutrition Development 41, 126 (Erratum 41, 377).
Gilbert, R, Cohen, JA, Pardo, S, Basu, A, Fischman, DA 1999. Identification of the A-band localization domain of myosin binding proteins C and H (MyBP-C, MyBP-H) in skeletal muscle. Journal of Cell Science 112, 6979.
Golenhofen, N, Perng, MD, Quinlan, RA, Drenckhahn, D 2004. Comparison of the small heat shock proteins alpha B-crystallin, MKBP, HSP25, HSP20, and cvHSP in heart and skeletal muscle. Histochemistry and Cell Biology 122, 415425.
Guillemin, N 2010. Marqueurs protéiques de la tendreté de la viande bovine: étude prédictive et fonctionnelle. Université Blaise Pascal, Clermont-Ferrand, France.
Guillemin, N, Meunier, B, Jurie, C, Cassar-Malek, I, Hocquette, JF, Levéziel, H, Picard, B 2009a. Validation of a Dot-blot quantitative technique for large-scale analysis of beef tenderness biomarkers. Journal of Physiology and Pharmacology 60, 9197.
Guillemin, N, Cassar-Malek, I, Hocquette, JF, Jurie, C, Micol, D, Listrat, A, Levéziel, H, Renand, G, Picard, B 2009b. La maîtrise de la tendreté de la viande bovine: identification de marqueurs biologiques. INRA Productions Animales 22, 331344.
Guillemin, N, Jurie, C, Renand, G, Hocquette, JF, Micol, D, Lepetit, J, Levéziel, H, Picard, B 2010. Tenderness phenotypic and proteomic markers. Journal of Animal Science (in press).
Herrmann, H, Bar, H, Kreplak, L, Strelkov, SV, Aebi, U 2007. Intermediate filaments: from cell architecture to nanomechanics. Nature Reviews Molecular Cell Biology 8, 562573.
Hocquette, JF, Lehnert, SA, Barendse, W, Cassar-Malek, I, Picard, B 2007. Recent advances in cattle functional genomics and their application to beef quality. Animal 1, 159173.
Jia, X, Hollung, K, Therkildsen, M, Hildrum, KI, Bendixen, E 2006. Proteome analysis of early post-mortem changes in two bovine muscle types: M. longissimus dorsi and M. semitendinosis. Proteomics 6, 936944.
Jia, X, Veiseth-Kent, E, Grove, H, Kuziora, P, Aass, L, Hildrum, KI, Hollung, K 2009. Peroxiredoxin-6 – a potential protein marker for meat tenderness in bovine M. Longissimus thoracis muscle. Journal of Animal Science 87, 23912399.
Junn, E, Taniguchi, H, Jeong, BS, Zhao, X, Ichijo, H, Mouradian, MM 2005. Interaction of DJ-1 with Daxx inhibits apoptosis signal-regulating kinase 1 activity and cell death. Proceedings of the National Academy of Sciences of the United States of America 102, 96919696.
Jurie, C, Robelin, J, Picard, B, Renand, G, Geay, Y 1995. Inter-animal variation in the biological characteristics of muscle tissue in male Limousin cattle. Meat Science 39, 415425.
Jurie, C, Picard, B, Hocquette, JF, Dransfield, E, Micol, D, Listrat, A 2007. Muscle and meat quality characteristics of Holstein and Salers cull cows. Meat Science 77, 459466.
Kaul, SC, Deocaris, CC, Wadhwa, R 2007. Three faces of mortalin: a housekeeper, guardian and killer. Experimental Gerontology 42, 263274.
Kiang, JG, Tsokos, GC 1998. Heat shock protein 70 kDa: molecular biology, biochemistry, and physiology. Pharmacology & Therapeutics 80, 183201.
Kubo, E, Hasanova, N, Tanaka, Y, Fatma, N, Takamura, Y, Singh, DP, Akagi, Y 2010. Protein expression profiling of lens epithelial cells from Prdx6-depleted mice and their vulnerability to UV radiation exposure. American Journal of Physiology – Cell Physiology 298, C342C354.
Lametsch, R, Karlsson, A, Rosenvold, K, Andersen, HJ, Roepstorff, P, Bendixen, E 2003. Postmortem proteome changes of porcine muscle related to tenderness. Journal of Agricultural and Food Chemistry 51, 69926997.
Laufen, T, Mayer, MP, Beisel, C, Klostermeier, D, Mogk, A, Reinstein, J, Bukau, B 1999. Mechanism of regulation of Hsp70 chaperones by DnaJ cochaperones. Proceedings of the National Academy of Sciences of the United States of America 96, 54525457.
Lee, CE, McArdle, A, Griffiths, RD 2007. The role of hormones, cytokines and heat shock proteins during age-related muscle loss. Clinical Nutrition 26, 524534.
Lefaucheur, L 2010. A second look into fibre typing – relation to meat quality. Meat Science 84, 257270.
Moradas-Ferreira, P, Costa, V, Piper, P, Mager, W 1996. The molecular defences against reactive oxygen species in yeast. Molecular Microbiology 19, 651658.
Morris, CA, Cullen, NG, Hickey, SM, Dobbie, PM, Veenvliet, BA, Manley, TR, Pitchford, WS, Kruk, ZA, Bottema, CD, Wilson, T 2006. Genotypic effects of calpain 1 and calpastatin on the tenderness of cooked M. longissimus dorsi steaks from Jersey × Limousin, Angus and Hereford-cross cattle. Animal Genetics 37, 411414.
Paulin, D, Li, ZL 2004. Desmin: a major intermediate filament protein essential for the structural integrity and function of muscle. Experimental Cell Research 301, 17.
Picard, B, Barboiron, C, Duris, MP, Gagniere, H, Jurie, C, Geay, Y 1999. Electrophoretic separation of bovine muscle myosin heavy chain isoforms. Meat Science 53, 17.
Picard, B, Berri, C, Lefaucheur, L, Molette, C, Sayd, T, Terlouw, C 2010. Skeletal muscle proteomics in livestock production. Briefings in Functional Genomics 9, 259278.
Picard, B, Jurie, C, Bauchart, D, Dransfield, E, Ouali, A, Martin, JF, Jailler, R, Lepetit, J, Culioli, J 2007. Caractéristiques des muscles et de la viande des principales races bovines allaitantes du Massif Central. Sciences des Aliments 27, 168180.
Schreurs, NM, Garcia, F, Jurie, C, Agabriel, J, Micol, D, Bauchart, D, Listrat, A, Picard, B 2008. Meta-analysis of the effect of animal maturity on muscle characteristics in different muscles, breeds, and sexes of cattle. Journal of Animal Science 86, 28722887.
Sun, Y, MacRae, TH 2005. Small heat shock proteins: molecular structure and chaperone function. Cellular and Molecular Life Sciences 62, 24602476.
Totland, GK, Kryvi, H 1991. Distribution patterns of muscle fibre types in major muscles of the bull (Bos taurus). Anatomy and Embryology 184, 441450.
Veysset, P, Bebin, D, Lherm, M 2005. Adaptation to Agenda 2000 (CAP reform) and optimisation of the farming system of French suckler cattle farms in the Charolais area: a model-based study. Agricultural Systems 83, 179202.
Vicart, P, Caron, A, Guicheney, P, Li, ZL, Prevost, MC, Faure, A, Chateau, D, Chapon, F, Tome, F, Dupret, JM, Paulin, D, Fardeau, M 1998. A missense mutation in the alpha B-crystallin chaperone gene causes a desmin-related myopathy. Nature Genetics 20, 9295.
Voss, MR, Stallone, JN, Li, M, Cornelussen, RNM, Knuefermann, P, Knowlton, AA 2003. Gender differences in the expression of heat shock proteins: the effect of estrogen. American Journal of Physiology – Heart and Circulatory Physiology 285, H687H692.
Wang, X, Phelan, SA, Forsman-Semb, K, Taylor, EF, Petros, C, Brown, A, Lerner, CP, Paigen, B 2003. Mice with targeted mutation of peroxiredoxin 6 develop normally but are susceptible to oxidative stress. Journal of Biological Chemistry 278, 2517925190.

Keywords

Variations in the abundance of 24 protein biomarkers of beef tenderness according to muscle and animal type

  • N. Guillemin (a1), C. Jurie (a1), I. Cassar-Malek (a1), J.-F. Hocquette (a1), G. Renand (a2) and B. Picard (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed