Skip to main content Accessibility help
×
Home

Repeated acidosis challenges and live yeast supplementation shape rumen microbiota and fermentations and modulate inflammatory status in sheep

  • M. Silberberg (a1) (a2), F. Chaucheyras-Durand (a3) (a4), L. Commun (a1) (a5), M. M. Mialon (a1) (a2), V. Monteils (a1) (a2) (a6) (a7) (a8), P. Mosoni (a4), D. P. Morgavi (a1) (a2) and C. Martin (a1) (a2)...

Abstract

This study aimed to investigate the impact of repeated acidosis challenges (ACs) and the effect of live yeast supplementation (Saccharomyces cerevisiae I-1077, SC) on rumen fermentation, microbial ecosystem and inflammatory response. The experimental design involved two groups (SC, n=6; Control, n=6) of rumen fistulated wethers that were successively exposed to three ACs of 5 days each, preceded and followed by resting periods (RPs) of 23 days. AC diets consisted of 60% wheat-based concentrate and 40% hay, whereas RPs diets consisted of 20% concentrate and 80% hay. ACs induced changes in rumen fermentative parameters (pH, lactate and volatile fatty-acid concentrations and proportions) as well as in microbiota composition and diversity. The first challenge drove the fermentation pattern towards propionate. During successive challenges, rumen pH measures worsened in the control group and the fermentation profile was characterised by a higher butyrate proportion and changes in the microbiota. The first AC induced a strong release of rumen histamine and lipopolysaccharide that triggered the increase of acute-phase proteins in the plasma. This inflammatory status was maintained during all AC repetitions. Our study suggests that the response of sheep to an acidosis diet is greatly influenced by the feeding history of individuals. In live yeast-supplemented animals, the first AC was as drastic as in control sheep. However, during subsequent challenges, yeast supplementation contributed to stabilise fermentative parameters, promoted protozoal numbers and decreased lactate producing bacteria. At the systemic level, yeast helped normalising the inflammatory status of the animals.

Copyright

Corresponding author

Footnotes

Hide All
*

Both authors contributed equally to the present work.

Footnotes

References

Hide All
AlZahal, O, Kebreab, E, France, J and McBride, BW 2007. A mathematical approach to predicting biological values from ruminal pH measurements. Journal of Dairy Science 90, 37773785.
AOAC 1990. Official methods of analysis. Association of Official Analitical Chemistry, Arlington, VA.
Brossard, L, Martin, C and Michalet-Doreau, B 2003. Ruminal fermentative parameters and blood acido-basic balance changes during the onset and recovery of induced latent acidosis in sheep. Animal Research 52, 513530.
Brossard, L, Martin, C, Chaucheyras-Durand, F and Michalet-Doreau, B 2004. Protozoa involved in butyric rather than lactic fermentative pattern during latent acidosis in sheep. Reproduction Nutrition Development 44, 195206.
Brossard, L, Chaucheyras-Durand, F, Michalet-Doreau, B and Martin, C 2006. Dose effect of live yeasts on rumen microbial communities and fermentations during butyric latent acidosis in sheep: new type of interaction. Animal Science 82, 829836.
Bryant, M and Burkey, L 1953. Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. Journal of Dairy Science 36, 205217.
Chaucheyras-Durand, F, Walker, ND and Bach, A 2008. Effects of active dry yeasts on the rumen microbial ecosystem: past, present, and future. Animal Feed Science and Technology 145, 126.
Chaucheyras, F and Fonty, G 2006. Effects and modes of action of live yeasts in the rumen. Biologia, Bratislava 61, 741750.
Clarke, KR and Owens, NJ 1983. A simple and versatile micro-computer program for the determination of “Most Probable Number”. Journal of Microbiological Methods 1, 133137.
Commun, L, Silberberg, M, Mialon, MM, Martin, C and Veissier, I 2012. Behavioural adaptations of sheep to repeated acidosis challenges and effect of yeast supplementation. Animal 6, 20112022.
Denman, SE and McSweeney, CS 2006. Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. Fems Microbiology Ecology 58, 572582.
Desnoyers, M, Giger-Reverdin, S, Bertin, G, Duvaux-Ponter, C and Sauvant, D 2009. Meta-analysis of the influence of Saccharomyces cerevisiae supplementation on ruminal parameters and milk production of ruminants. Journal of Dairy Science 92, 16201632.
Dohme, F, DeVries, TJ and Beauchemin, KA 2008. Repeated ruminal acidosis challenges in lactating dairy cows at high and low risk for developing acidosis: ruminal pH. Journal of Dairy Science 91, 35543567.
Edwards, JE, Huws, SA, Kim, EJ and Kingston-Smith, AH 2007a. Characterization of the dynamics of initial bacterial colonization of nonconserved forage in the bovine rumen-corrigendum. Fems Microbiology Ecology 62, 12.
Edwards, JE, Huws, SA, Kim, EJ and Kingston-Smith, AH 2007b. Characterization of the dynamics of initial bacterial colonization of nonconserved forage in the bovine rumen. Fems Microbiology Ecology 62, 323335.
Emmanuel, DGV, Madsen, KL, Churchill, TA, Dunn, SM and Ametaj, BN 2007. Acidosis and lipopolysaccharide from Escherichia coli B:055 cause hyperpermeability of rumen and colon tissues. Journal of Dairy Science 90, 55525557.
Garner, MR, Flint, JF and Russell, JB 2002. Allisonella histaminiformans gen. nov., sp. nov. A novel bacterium that produces histamine, utilizes histidine as its sole energy source, and could play a role in bovine and equine laminitis. Systematic Applied Microbiology 25, 498506.
Gifford, CA, Holland, BP, Mills, RL, Maxwell, CL, Farney, JK, Terrill, SJ, Step, DL, Richards, CJ, Robles, LOB and Krehbiel, CR 2012. Growth and development symposium: impacts of inflammation on cattle growth and carcass merit. Journal of Animal Science 90, 14381451.
Gozho, GN, Plaizier, JC, Krause, DO, Kennedy, AD and Wittenberg, KM 2005. Subacute ruminal acidosis induces ruminal lipopolysaccharide endotoxin release and triggers an inflammatory response. Journal of Dairy Science 88, 13991403.
INRA 2007a. Valeur alimentaire des fourrages et des matières premières: tables et prévisions. In Alimentation des bovins, ovins et caprins (p. 167. Quae. Institut National de la Recherche Agronomique, Paris, France.
INRA 2007b. Alimentation des bovins, ovins et caprins. INRA, Versailles.
Keunen, JE, Plaizier, JC, Kyriazakis, L, Duffield, TF, Widowski, TM, Lindinger, MI and McBride, BW 2002. Effects of a subacute ruminal acidosis model on the diet selection of dairy cows. Journal of Dairy Science 85, 33043313.
Khafipour, E, Krause, DO and Plaizier, JC 2009a. A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation. Journal of Dairy Science 92, 10601070.
Khafipour, E, Krause, DO and Plaizier, JC 2009b. Alfalfa pellet-induced subacute ruminal acidosis in dairy cows increases bacterial endotoxin in the rumen without causing inflammation. Journal of Dairy Science 92, 17121724.
Khafipour, E, Li, S, Plaizier, JC and Krause, DO 2009c. Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis. Applied Environmental Microbiology 75, 71157124.
Kleen, JL and Cannizzo, C 2012. Incidence, prevalence and impact of SARA in dairy herds. Animal Feed Science and Technology 172, 48.
Krause, KM, Garrett, R and Oetzel, GR 2006. Understanding and preventing subacute ruminal acidosis in dairy herds: a review. Animal Feed Science and Technology 126, 215236.
Lettat, A, Noziere, P, Silberberg, M, Morgavi, DP, Berger, C and Martin, C 2010. Experimental feed induction of ruminal lactic, propionic, or butyric acidosis in sheep. Journal of Animal Science 88, 30413046.
Li, RW, Wu, ST, Baldwin, RL, Li, WZ and Li, CJ 2012. Perturbation dynamics of the rumen microbiota in response to exogenous butyrate. PLoS One 7.
Michelland, RJ, Combes, S, Monteils, V, Cauquil, L, Gidenne, T and Fortun-Lamothe, L 2009. Molecular analysis of the bacterial community in digestive tract of rabbit. Anaerobe 16, 6165.
Monteils, V, Rey, M, Silberberg, M, Cauquil, L and Combes, S 2012. Modification of activities of the ruminal ecosystem and its bacterial and protozoan composition during repeated dietary changes in cows. Journal of Animal Science 90, 44314440.
Morgavi, DP, Boudra, H, Jouany, JP and Graviou, D 2003. Prevention of patulin toxicity on rumen microbial fermentation by SH-containing reducing agents. Journal of Agriculture and Food Chemistry 51, 69066910.
Mosoni, P, Chaucheyras-Durand, F, Bera-Maillet, C and Forano, E 2007. Quantification by real-time PCR of cellulolytic bacteria in the rumen of sheep after supplementation of a forage diet with readily fermentable carbohydrates: effect of a yeast additive. Journal of Applied Microbiology 103, 26762685.
Mosoni, P, Martin, C, Forano, E and Morgavi, DP 2011. Long-term defaunation increases the abundance of cellulolytic ruminococci and methanogens but does not affect the bacterial and methanogen diversity in the rumen of sheep. Journal of Animal Science 89, 783791.
Nagaraja, TG and Titgemeyer, EC 2007. Ruminal acidosis in beef cattle: the current microbiological and nutritional outlook. Journal of Dairy Science 90 (suppl. 1), E17E38.
Plaizier, JC, Krause, DO, Gozho, GN and McBride, BW 2008. Subacute ruminal acidosis in dairy cows: the physiological causes, incidence and consequences. The Veterinary Journal 176, 2131.
Stevenson, DM and Weimer, PJ 2007. Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR. Applied Microbiology and Biotechnology 75, 165174.
Zebeli, Q and Metzler-Zebeli, BU 2012. Interplay between rumen digestive disorders and diet-induced inflammation in dairy cattle. Research in Veterinary Science 93, 10991108.
Zebeli, Q, Metzler-Zebeli, BU and Ametaj, BN 2012. Meta-analysis reveals threshold level of rapidly fermentable dietary concentrate that triggers systemic inflammation in cattle. Journal of Dairy Science 95, 26622672.

Keywords

Type Description Title
WORD
Supplementary materials

Silberberg Supplementary Material
Supplementary Material

 Word (1.1 MB)
1.1 MB

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed