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Pyrethroids cypermethrin, deltamethrin and fenvalerate have different effects on in vitro maturation of pig oocytes at different stages of growth

  • J. Petr (a1), E. Chmelíková (a2), T. Žalmanová (a2), L. Tůmová (a2), K. Kheilová (a2), V. Kučerová-Chrpová (a2) and F. Jílek (a2)...


Pesticides can significantly harm reproduction in animals and people. Pyrethroids are often used as insecticides, and their toxicity for mammals is considered to be low. However, cypermethrin, deltamethrin and fenvalerate – as potent specific inhibitors of protein phosphatase calcineurin – can influence the meiosis of mammalian oocytes. The objective of this study was to evaluate the effects of these pyrethroids on the in vitro maturation of pig oocytes at different levels of meiotic competence. Under the tested concentrations, cypermethrin, deltamethrin and fenvalerate neither had a significant effect on the viability of oocytes nor did they induce significant degeneration of oocytes. However, these pyrethroids significantly affected meiotic maturation. The effects depended on the stage of meiotic competence of the oocytes. Maturation of growing pig oocytes with partial meiotic competence was induced. On the other hand, in fully grown pig oocytes with full meiotic competence, maturation in vitro was delayed. The specificity of these effects was further supported by the same effect of non-pyrethroidal inhibitors of calcineurin – cyclosporin A or hymenistatin I – on the maturation of oocytes with different levels of meiotic competence. However, pyrethroids, which do not inhibit calcineurin – allethrin or permethrin – had no effect on pig oocyte maturation. We demonstrated a significant effect of pyrethroids on the maturation of mammalian oocytes under in vitro conditions. This indicates that exposure to these substances could affect the fertility of people or animals.


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Bouwman, H, Seereda, B, Meinhadt, HM 2006. Simultaneous presence of DDT and pyrethroid residues in human breast milk from malaria endemic area in South Africa. Environmental Pollution 144, 902917.
Casas, E, Bonilla, E, Ducolomb, Y, Betancourt, M 2010. Differential effects of herbicides atrazine and fenoxaprop-ethyl, and insecticides diazinon and malathion, on viability and maturation of porcine oocytes in vitro. Toxicology in Vitro 24, 224230.
Chang, KC 2007. Key signalling factors and pathways in the molecular determination of skeletal muscle phenotype. Animal 5, 681698.
Cohen, PTW 2002. Protein phosphatase 1 – targeted in many directions. Journal of Cell Science 2, 241256.
Coull, GD, Speake, BK, Staines, ME, Broadbent, PJ, McEvoy, TG 1998. Lipid and Fatty acid composition of zona-intact sheep oocytes. Theriogenology 49, 179.
Elflein, L, Berger-Preiss, E, Preiss, A, Elend, M, Levsen, K, Wunsch, G 2003. Human biomonitoring of pyrethrum and pyrethroid insecticides used indoors: determination of metabolites E-cis/trans chrysanthemum dicarboxylic acid in human urine by gas chromatography–mass spectrometry with negative chemical ionization. Journal of Chromatography B 795, 195207.
Enan, E, Matsumura, F 1992. Specific-inhibition of calcineurin by type-II synthetic pyrethroid insecticides. Biochemical Pharmacology 43, 17771784.
Ferguson, EM, Leese, HJB 1999. Triglyceride content of bovine oocytes and early embryos. Journal of Reproduction and Fertility 116, 373378.
Gorr, IH, Reis, A, Boos, D, Wuhr, M, Madgwick, S, Jones, KT, Stemmann, O 2006. Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes. Nature Cell Biology 9, 1035U120.
Hampl, A, Eppig, JJ 1995. Analysis of the mechanism(s) of metaphase-I arrest in maturing mouse oocytes. Development 121, 925933.
Heudorf, U, Angerer, J 2001. Metabolites of pyrethroid insecticides in urine specimens: current exposure in an urban population in Germany. Environmental Health Perspectives 109, 213217.
Julien, R, Adamkiewicz, G, Levy, JI, Bennett, D, Nishioka, M, Spengler, JD 2008. Pesticide loadings of select organophosphate and pyrethroid pesticides in urban public housing. Journal of Exposure Science and Environmental Epidemiology 18, 167174.
Kim, K-B, Anand, SS, Kim, HJ, White, CA, Bruckner, JV 2008. Toxikinetics and tissue distribution of deltamethrin in adult Sprague-Dawley Rats. Toxicological Sciences 101, 197205.
Kim, K-B, Anand, SS, Kim, HJ, White, CA, Fisher, JW, Tornero-Velez, R, Bruckner, JV 2010. Age, dose, and time dependency of plasma and tissue distribution of deltamethrin in immature rats. Toxicological Sciences 115, 354368.
Leng, G, Kühn, KH, Idel, H 1997. Biological monitoring of pyrethroids in blood and pyrethroid metabolites in urine: applications and limitations. Science of the Total Environment 199, 173181.
Liao, HT, Hsieh, CJ, Chiang, SY, Lin, MH, Chen, PC, Wu, KY 2011. Simultaneous analysis of chlorpyrifos and cypermethrin in cord blood plasma by online solid-phase extraction coupled with chromatography-heated electrospray ionization tandem mass spectrometry. Journal of Chromatography B 879, 19611966.
Lowenstein, JE, Cohen, AI 1964. Dry mass, lipid content and protein content of the intact and zona-free mouse ovum. Journal of Embryology and Experimental Morphology 12, 113121.
McEvoy, TG, Coull, GD, Broadbent, PJ, Hutchinson, JS, Speake, BK 2000. Fatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida. Journal of Reproduction and Fertility 11, 163170.
Meeker, JD, Barr, DB, Hauser, R 2009. Pyrethroid insecticide metabolites are associated with serum hormone levels in adult men. Reproductive Toxicology 27, 155160.
Mochida, S, Hunt, T 2007. Calcineurin is required to release Xenopus egg extracts from meiotic M phase. Nature 449, 336340.
Motlík, J, Fulka, J 1986. Factors affecting meiotic competence in pig oocytes. Theriogenology 25, 8796.
Perger, G, Szadkowski, D 1994. Toxicology of pyrethroids and their relevance to human health. Annals of Agricultural and Environmental Medicine 1, 1117.
Prasanthi, K, Muralidhara, , Rajini, PS 2005. Morphological and biochemical perturbations in rat erythrocytes following in vitro exposure to fenvalerate and its metabolite. Toxicology in Vitro 19, 449456.
Ramesh, A, Ravi, PE 2004. Negative ion chemical ionization-gas chromatographic-mass spectrometric determination of residues of different pyrethroid insecticides in whole blood serum. Journal of Analytical Toxicology 28, 260266.
Rime, H, Ozon, R 1990. Protein phosphatases are involved in the in vivo activation of histone H1 kinase in mouse oocyte. Developmental Biology 141, 115122.
Rothwell, JT, Burnett, TJ, Hacket, K, Chevis, R, Lowe, LB 2001. Residues of zeta-cypermethrin in bovine tissues and milk following pour-on and spray application. Pest Management Science 57, 993999.
Rozinek, J, Vaňourková, Z, Sedmíková, M, Lánská, V, Petr, J, Rajmon, R, Jílek, F 2003. Ultrastructural localisation of calcium deposits in pig oocytes maturing in vitro: effects of verapamil. Zygote 11, 253260.
Sassine, A, Moura, S, Morais Léo, V, Vega Bustillos, O 2004. Cypermethrin residues determination in the milk of lactating dairy cows by gas chromatography-ion trap mass spectrometry. Journal of Analytical Toxicology 28, 238241.
Soderlund, DM 2010. State-dependent modification of voltage-gated sodium channels by pyrethroids. Pesticide Biochemistry and Physiology 97, 7886.
Soderlund, DM, Clark, JM, Sheets, LP, Mullin, LS, Piccirillo, VJ, Sargent, D, Stevens, JT, Weiner, ML 2002. Mechanisms of pyrethroid neurotoxicity: implications for cumulative risk assessment. Toxicology 171, 359.
Takeo, S, Hawley, RS, Aigaki, T 2010. Calcineurin and its regulation by Sra/RCAN is required for completion of meiosis in Drosophila. Developmental Biology 344, 957967.
Taplin, D, Meinking, TL 1987. Pyrethrins and pyrethroids for the treatment of scabies and pediculosis. Seminars in Dermatology 6, 125135.
Tichovská, H, Petr, J, Chmelíková, E, Sedmíková, M, Tůmová, L, Krejčová, M, Dörflerová, A, Rajmon, R 2011. Nitric oxide and meiotic competence of porcine oocytes. Animal 9, 13981405.
Tosti, E, Boni, R 2004. Electrical events during gamete maturation and fertilization in animals and humans. Human Reproduction Update 10, 5365.
Wassarman, PM 1988. The mammalian ovum. In The physiology of reproduction (ed. E Knobil and J Neil), pp. 69102. Raven Press, New York.
Weston, DP, Lydy, MJ 2010. Urban and agricultural sources of pyrethroid insecticides to the Sacramento–San Joaquin Delta of California. Environmental Science and Technology 44, 18331840.
Wolansky, MJ, Harrill, JA 2008. Neurobehavioral toxicology of pyrethroid insecticides in adult animals: a critical review. Neurotoxicology and Teratology 30, 5578.
Yakel, JL 1997. Calcineurin regulation of synaptic function: from ion channels to transmitter release and gene transcription. Trends in Pharmacological Sciences 18, 124134.
Yu, HT 2002. Regulation of APC-Cdc20 by the spindle checkpoint. Current Opinion in Cell Biology 14, 706714.
Yuan, C, Wang, C, Gao, SQ, Kong, TT, Chen, L, Li, XF, Song, L, Wang, YB 2010. Effects of permethrin, cypermethrin and 3-phenoxybenzoic acid on rat sperm motility in vitro evaluated with computer-assisted sperm analysis. Toxicology in Vitro 24, 382386.


Pyrethroids cypermethrin, deltamethrin and fenvalerate have different effects on in vitro maturation of pig oocytes at different stages of growth

  • J. Petr (a1), E. Chmelíková (a2), T. Žalmanová (a2), L. Tůmová (a2), K. Kheilová (a2), V. Kučerová-Chrpová (a2) and F. Jílek (a2)...


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