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N-alkanes v. ytterbium/faecal index as two methods for estimating herbage intake of dairy cows fed on diets differing in the herbage : maize silage ratio and feeding level

Published online by Cambridge University Press:  30 August 2011

E. Pérez-Ramírez ,
J. L. Peyraud  and
R. Delagarde
E. Pérez-Ramírez*
Affiliation:
INRA, UMR1080, Production du Lait, F-35590 Saint-Gilles, France Agrocampus Ouest, UMR1080, Production du Lait, F-35000 Rennes, France
J. L. Peyraud
Affiliation:
INRA, UMR1080, Production du Lait, F-35590 Saint-Gilles, France Agrocampus Ouest, UMR1080, Production du Lait, F-35000 Rennes, France
R. Delagarde
Affiliation:
INRA, UMR1080, Production du Lait, F-35590 Saint-Gilles, France Agrocampus Ouest, UMR1080, Production du Lait, F-35000 Rennes, France
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Abstract

The aim of this study was to compare the n-alkanes and the ytterbium (Yb)/faecal index techniques as two methods for estimating the herbage intake of dairy cows fed indoors on different herbage : supplement ratios and feeding levels. The supplement was a mixture of maize silage and soyabean meal (ratio of 87 : 13 on a dry matter (DM) basis). In all, four treatments were studied. The herbage : supplement ratio in the diet was 25 : 75, 50 : 50, 75 : 25 and 50 : 50 for treatments 1, 2, 3 and 4, respectively. Animals were offered for treatments 1, 2 and 3, 100% of ad libitum intake measured before the experiment and 70% of ad libitum intake for treatment 4. Cows were fed herbage in the morning and supplement in the evening. A total of six lactating Holstein dairy cows were used in a 4 × 4 Latin square with four 14-day periods. Herbage and supplement intakes, faecal output (FO), in vivo organic matter (OM) digestibility and faecal recovery of markers were measured on the last 5 days of each period. Intake was estimated with the two methods and from two faecal sampling techniques, that is, total faecal collection v. grab sampling during milking. Mean herbage intake as fed, or estimated from n-alkanes or from the Yb/faecal index was 7.7, 8.1 and 10.2 kg DM, respectively. The mean prediction error, expressed as a fraction of actual herbage intake, was 0.10 and 0.50 for the n-alkanes and Yb/faecal index methods, respectively. The n-alkanes method clearly showed much better accuracy than the Yb/faecal index method for estimating intake, irrespective of the faecal sampling method, herbage : silage proportion or feeding level. For the n-alkanes method, herbage intake was slightly overestimated (7%) when herbage proportion in the diet was high, due to a ratio of faecal C33 : C32 recovery >1. The high bias for the Yb/faecal index was due to the cumulative effect of overestimation of FO (mean recovery of Yb = 0.92) and underestimation of the diet indigestible fraction (−8%). Between-treatment variations of FO were on average well estimated by Yb. Between-treatment variations of OM digestibility estimated using the faecal index technique were lower than those observed in vivo. It is concluded that intake of grazing dairy cows receiving high levels of maize silage supplement should be estimated using the n-alkanes method.

Keywords

intakemethodologydairy cown-alkanesytterbium
Type
Full Paper
Information
animal , Volume 6 , Issue 2 , 03 January 2012 , pp. 232 - 244
Copyright
Copyright © The Animal Consortium 2011

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References

Association Française de Normalisation 1997. Aliments des animaux–Dosage de l'azote–Méthode par combustion (DUMAS)–NF V18–120; Dosage des cendres brutes–NF V18–101. AFNOR Editions, Saint-Denis La Plaine, France.Google Scholar
Aufrère, J, Michalet-Doreau, B 1988. Comparison of methods for predicting digestibility of feeds. Animal Feed Science and Technology 20, 203–218.CrossRefGoogle Scholar
Bartiaux-Thill, N, François, E 1980. Utilisation de l'oxyde de chrome dans la mesure de la consommation à l'herbage. Bulletin des Recherches Agronomiques de Gembloux 15, 107–120.Google Scholar
Bibby, J, Toutenburg, H 1977. Prediction and improved estimation in linear models. John Wiley and Sons, Chichester, UK.Google Scholar
Carruthers, VR, Bryant, AM 1983. Evaluation of the use of chromic oxide to estimate the feed intake of dairy cows. New Zealand Journal of Agricultural Research 26, 183–186.CrossRefGoogle Scholar
Chenost, M, Grenet, E, Demarquilly, C, Béranger, C 1985. Influence of supplementation on herbage digestibility and on faeces characteristics with sheep. Proceedings of the 15th International Grassland Congress, Kyoto, Japan, pp. 985–986.Google Scholar
Costa, M 1997. Toxicity and carcinogenicity of Cr(VI) in animal models and humans. Critical Review of Toxicology 27, 431–442.CrossRefGoogle ScholarPubMed
Decruyenaere, V, Peters, M, Stilmant, D, Lecomte, P, Dardenne, P 2003. Near infrared reflectance spectroscopy applied to faeces to predict dry matter intake of sheep under grazing, comparison with n-alkanes and direct biomass measurement methods. Tropical and Subtropical Agroecosystems 3, 471–475.Google Scholar
Delagarde, R, Peyraud, JL, Delaby, L 1999. Influence of carbohydrate or protein supplementation on intake, behaviour and digestion in dairy cows strip-grazing low-nitrogen fertilized perennial ryegrass. Annales de Zootechnie 48, 81–96.CrossRefGoogle Scholar
Delagarde, R, Pérez-Ramírez, E, Peyraud, JL 2010. Ytterbium oxide has the same accuracy as chromic oxide for estimating variations of faecal dry matter output in dairy cows fed a total mixed ration at two feeding levels. Animal Feed Science and Technology 161, 121–131.CrossRefGoogle Scholar
Dove, H, Mayes, RW 1996. Plant wax components: a new approach to estimating intake and diet composition in herbivores. Journal of Nutrition 126, 13–26.CrossRefGoogle ScholarPubMed
Dove, H, Freer, M, Foot, JZ 2000. The nutrition of grazing ewes during pregnancy and lactation: a comparison of alkane-based and chromium/in vitro-based estimates of herbage intake. Australian Journal of Agricultural Research 51, 765–777.CrossRefGoogle Scholar
Dove, H, Mayes, RW, Freer, M, Coombe, JC, Foot, JZ 1989. Faecal recoveries of the alkanes of plant cuticular waxes in penned and in grazing sheep. Proceedings of the 16th International Grassland Congress, Nice, France, pp. 1093–1094.Google Scholar
Elwert, C, Kluth, H, Rodehutscord, M 2004. Effect of variable intake of alfalfa and wheat on faecal alkane recoveries and estimates of roughage intake in sheep. Journal of Agricultural Science 142, 213–223.CrossRefGoogle Scholar
Ferreira, LMM, Oliván, M, Rodrigues, MAM, Osoro, K, Dove, H, Dias-da-Silva, A 2004. Estimation of feed intake by cattle using controlled-release capsules containing n-alkanes or chromium sesquioxide. Journal of Agricultural Science 142, 225–234.CrossRefGoogle Scholar
Fries, GF, Marrow, GS, Snow, PA 1982. Soil ingestion by dairy cattle. Journal of Dairy Science 65, 611–618.CrossRefGoogle ScholarPubMed
García, SC, Holmes, CW, Hodgson, J, MacDonald, A 2000. The combination of the n-alkanes and 13C techniques to estimate individual dry matter intakes of herbage and maize silage by grazing dairy cows. Journal of Agricultural Science, Cambridge 135, 47–55.CrossRefGoogle Scholar
Gordon, IJ 1995. Animal-based techniques for grazing ecology research. Small Ruminant Research 16, 203–214.CrossRefGoogle Scholar
Institut National de la Recherche Agronomique (INRA) 2007. Alimentation des bovins, ovins et caprins. Besoins de animaux, valeurs des aliments, Tables INRA 2007. Editions QUAE, Versailles, France.Google Scholar
Malossini, F, Bovolenta, S, Piasentier, E, Piras, C, Martillotti, F 1996. Comparison of n-alkanes and chromium oxide methods for estimating herbage intake by grazing dairy cows. Animal Feed Science and Technology 61, 155–165.CrossRefGoogle Scholar
Mambrini, M, Peyraud, JL 1994. Mean retention time in digestive tract and digestion of fresh perennial ryegrass by lactating dairy cows: influence of grass maturity and comparison with a maize silage diet. Reproduction Nutrition Development 34, 9–23.CrossRefGoogle ScholarPubMed
Mayes, RW, Lamb, CS, Colgrove, PM 1986. The use of dosed and herbage n-alkanes as markers for the determination of herbage intake. Journal of Agricultural Science, Cambridge 107, 161–170.CrossRefGoogle Scholar
Mayes, RW, Lamb, CS, Colgrove, PM 1988. Digestion and metabolism of dosed even-chain and herbage odd-chain n-alkanes in sheep. Proceedings of the 12th General Meeting of the European Grassland Federation, Dublin, 159p.Google Scholar
Mélix, C, Peyraud, JL, Vérité, R 1987. Utilisation de l'oxyde de chrome chez les vaches laitières pour la prévision des quantités de fèces émises. I. Etude des variations du taux de récupération et ses conséquences sur l'estimation de la digestibilité et des quantités ingérées de rations d'herbe et d'ensilage de maïs. Reproduction Nutrition Development 27 (suppl.), 215–216.CrossRefGoogle Scholar
Minson, DJ, Raymond, WF 1958. Sources of error in the use of faecal index relationships. Experiments in Progress 10, 92–96.Google Scholar
Moran, JB, Lemerle, C, Trigg, TE 1987. Excretion patterns of chromium sesquioxide in dairy cows and sheep. Journal of the Australian Institute of Agricultural Science 53, 290–292.Google Scholar
Morenz, MJF, da Silva, JFC, Aroeira, LJM, Deresz, F, Vasquez, HM, Paciullo, DSC, Lopes, FCF, Elyas, ACW, Detmann, E 2006. Oxido de cromo e n-alcanos na estimativa do consumo de forragem de vacas em lactação em condições de pastejo. Revista Brasileira de Zootecnia 35, 1535–1542.CrossRefGoogle Scholar
Moshtaghi Nia, SA, Wittenberg, KM 2002. Evaluation of n-alkanes as markers for estimation of dry matter intake and digestibility in steers consuming all-forage or forage-concentrate diets. Canadian Journal of Animal Science 82, 419–425.CrossRefGoogle Scholar
Ohajuruka, OA, Palmquist, DL 1991. Evaluation of n-alkanes as digesta markers in dairy cows. Journal of Animal Science 69, 1726–1732.CrossRefGoogle ScholarPubMed
Oliván, M, Ferreira, LMM, Celaya, R, Osoro, K 2007. Accuracy of the n-alkane technique for intake estimates in beef cattle using different sampling procedures and feeding levels. Livestock Science 106, 28–40.CrossRefGoogle Scholar
Ørskov, ER, Enoser, C, Mason, VC, Mann, SO 1970. Influence of starch digestion in the large intestine of sheep on caecal fermentation, caecal microflora and faecal nitrogen excretion. Proceedings of the Journal of Nutrition 24, 671–672.CrossRefGoogle ScholarPubMed
Penning, PD 2004. Animal based techniques for estimating herbage intake. In Herbage intake handbook (ed. PD Penning), pp. 53–93. British Grassland Society, Reading, UK.Google Scholar
Pérez-Ramírez, E, Delagarde, R, Delaby, L 2008. Herbage intake and behavioural adaptation of grazing dairy cows by restricting time at pasture under two feeding regimes. Animal 2, 1384–1392.CrossRefGoogle ScholarPubMed
Peyraud, JL 1997. Techniques for measuring herbage intake of grazing ruminants: a review. In Managing high yielding dairy cows at pasture (ed. E Spörndly, E Burstedt and M Murphy), pp. 3–23. Swedish University of Agricultural Sciences, Uppsala, Sweden.Google Scholar
Piasentier, E, Bovolenta, S, Malossini, F, Susmel, P 1995. Comparison of n-alkanes or chromium oxide methods for estimation of herbage intake by sheep. Small Ruminant Research 18, 27–32.CrossRefGoogle Scholar
Prigge, EC, Varga, GA, Vicini, JL, Reid, RL 1981. Comparison of ytterbium chloride and chromium sesquioxide as fecal indicators. Journal of Animal Science 53, 1629–1633.CrossRefGoogle ScholarPubMed
Raymond, WF, Minson, DJ 1955. The use of chromic oxide for estimating the faecal production of grazing animals. Journal of the British Grassland Society 10, 282–296.CrossRefGoogle Scholar
Ribeiro Filho, HMN, Delagarde, R, Peyraud, JL 2005. Herbage intake and milk yield of dairy cows grazing perennial ryegrass swards or white clover/perennial ryegrass swards at low- and medium-herbage allowances. Animal Feed Science and Technology 119, 13–27.CrossRefGoogle Scholar
Siddons, RC, Paradine, DE, Beever, DE, Cornell, PR 1985. Ytterbium acetate as a particulate digesta flow marker. British Journal of Nutrition 54, 509–519.CrossRefGoogle ScholarPubMed
Statistical Analysis System Institute Inc 1987. SAS User's Guide. SAS Institute Inc, Cary, NC, USA.Google Scholar
Unal, Y, Garnsworthy, PC, Gorton, P 1997. The use of n-alkanes for prediction of intake in dairy cows. Proceedings of the British Society of Animal Science, Penicuik, UK. 137p.CrossRefGoogle Scholar
van Soest, PJ, Roberson, JB, Lewis, BA 1991. Carbohydrate methodology, metabolism, and nutritional implications in dairy cattle. Journal of Dairy Science 75, 2215–2225.Google Scholar
Valderrabano, J 1979. Techniques of measuring intake by grazing sheep. PhD, Reading University.Google Scholar
Vulich, SA, Hanrahan, JP 1995. Faecal sampling for the estimation of herbage intake using n- alkanes: evaluation of sample pooling and the use of rectal grab samples. Journal of Agricultural Science, Cambridge 124, 79–86.CrossRefGoogle Scholar
Vulich, SA, O'Riordan, EG, Hanrahan, JP 1991. Effect of litter size on herbage intake at pasture by ewes and their progeny. Animal Production 53, 191–197.Google Scholar