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Effects of maternal methyl donor on the pork characteristics of offspring pigs with prenatal exposure to bisphenol A

  • Y. Zhuo (a1), J. Wang (a1), H. Liu (a1), D. Mou (a1), T. Adebowale (a2), L. Che (a1), Z. Fang (a1), S. Xu (a1), G. Liu (a2), Y. Lin (a1), B. Feng (a1), N. Abdullah Al-Dhabi (a3), J. Li (a1), V. Duraipandiyan (a3) and D. Wu (a1)...

Abstract

To explore the effects of maternal nutrition on offspring muscle characteristics, a total of 56 sows were assigned to one of the four dietary groups during gestation: control (CON), or control diets supplemented with methyl donor (MET), bisphenol A (BPA), and combined BPA and MET (BPA+MET). Compared with CON offspring, MET offspring showed a higher meat redness value, but lower glycogen content in the longissimus thoracis (LT). Moreover, compared with CON offspring, MET offspring showed lower LT glycogen synthase (GS) mRNA levels at birth and the finishing stage, and increased methylation at the GS promoter. Prenatal BPA exposure reduced the pH and redness value of meat, but increased the lightness value, lactate content, glycolytic potential and lactate dehydrogenase (LDH) enzyme activity in the LT muscle. Prenatal BPA exposure increased LDH mRNA levels in the LT muscle at birth and the finishing stage, and reduced methylation at the LDH promoter. Thus, maternal MET affects muscle GS and LDH expression via DNA methylation, thereby resulting in persistent effects on pork quality.

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Yong Zhuo and Jun Wang contributed equally to this work.

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Anderson, OS, Sant, KE and Dolinoy, DC 2012. Nutrition and epigenetics: an interplay of dietary methyl donors, one-carbon metabolism and DNA methylation. The Journal of Nutritional Biochemistry 23, 853859.
Berkes, CA and Tapscott, SJ 2005. MyoD and the transcriptional control of myogenesis. Seminars in Cell & Developmental Biology 16, 585595.
Bertol, T, Braña, D, Ellis, M, Ritter, M, Peterson, B, Mendoza, O and McKeith, F 2011. Effect of feed withdrawal and dietary energy source on muscle glycolytic potential and blood acid-base responses to handling in slaughter-weight pigs. Journal of Animal Science 89, 15611573.
Brenet, F, Moh, M, Funk, P, Feierstein, E, Viale, AJ, Socci, ND and Scandura, JM 2011. DNA methylation of the first exon is tightly linked to transcriptional silencing. PloS One 6, e14524.
Brewer, M, Zhu, L, Bidner, B, Meisinger, D and McKeith, F 2001. Measuring pork color: effects of bloom time, muscle, pH and relationship to instrumental parameters. Meat Science 57, 169176.
Bowker, BC 2013. Meat Science And Muscle Biology Symposium: in utero factors that influence postnatal muscle growth, carcass composition, and meat quality. Journal of Animal Science 91, 14171418.
Calvisi, DF, Simile, MM, Ladu, S, Pellegrino, R, De Murtas, V, Pinna, F, Tomasi, ML, Frau, M, Virdis, P and De Miglio, MR 2007. Altered methionine metabolism and global DNA methylation in liver cancer: relationship with genomic instability and prognosis. International Journal of Cancer 121, 24102420.
Chao, HH, Zhang, XF, Chen, B, Pan, B, Zhang, LJ, Li, L, Sun, XF, Shi, QH and Shen, W 2012. Bisphenol A exposure modifies methylation of imprinted genes in mouse oocytes via the estrogen receptor signaling pathway. Histochemistry and Cell Biology 137, 249259.
Dolinoy, DC, Huang, D and Jirtle, RL 2007. Maternal nutrient supplementation counteracts bisphenol A-induced DNA hypomethylation in early development. Proceedings of the National Academy of Sciences 104, 1305613061.
Guay, F, Matte, JJ, Girard, CL, Palin, MF, Giguère, A and Laforest, JP 2002. Effects of folic acid and vitamin B 12 supplements on folate and homocysteine metabolism in pigs during early pregnancy. British Journal of Nutrition 88, 253263.
Hamilton, D, Miller, K, Ellis, M, McKeith, F and Wilson, E 2003. Relationships between longissimus glycolytic potential and swine growth performance, carcass traits, and pork quality. Journal of Animal Science 81, 22062212.
Jones, PA 2012. Functions of DNA methylation: islands, start sites, gene bodies and beyond. Nature Reviews Genetics 13, 484492.
Kruszynska, YT, Ciaraldi, TP and Henry, RR 2011. Regulation of glucose metabolism in skeletal muscle. In Comprehensive physiology. Handbook of physiology, the endocrine system, the endocrine pancreas and regulation of metabolism (ed. D M Pollock), pp. 579607. The American Physiological Society, Bethesda, Maryland, USA.
Laing, L, Viana, J, Dempster, E, Trznadel, M, Trunkfield, L, Webster, TU, van Aerle, R, Paull, G, Wilson, R and Mill, J 2016. Bisphenol A causes reproductive toxicity, decreases dnmt1 transcription, and reduces global DNA methylation in breeding zebrafish (Danio rerio). Epigenetics 11, 526538.
Law, JA and Jacobsen, SE 2010. Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nature Reviews Genetics 11, 204220.
Lebret, B, Meunier-Salaun, MC, Foury, A, Mormede, P, Dransfield, E and Dourmad, JY 2006. Influence of rearing conditions on performance, behavioral, and physiological responses of pigs to preslaughter handling, carcass traits, and meat quality. Journal of Animal Science 84, 24362447.
Leheska, J, Wulf, D and Maddock, R 2002. Effects of fasting and transportation on pork quality development and extent of postmortem metabolism. Journal of Animal Science 80, 31943202.
Lillycrop, KA and Burdge, GC 2012. Epigenetic mechanisms linking early nutrition to long term health. Best Practice & Research Clinical Endocrinology & Metabolism 26, 667676.
Liu, J, Lam, JB, Chow, KH, Xu, A, Lam, KS, Moon, RT and Wang, Y 2008. Adiponectin stimulates Wnt inhibitory factor-1 expression through epigenetic regulations involving the transcription factor specificity protein 1. Carcinogenesis 29, 21952202.
Ma, Y, Xia, W, Wang, D, Wan, Y, Xu, B, Chen, X, Li, Y and Xu, S 2013. Hepatic DNA methylation modifications in early development of rats resulting from perinatal BPA exposure contribute to insulin resistance in adulthood. Diabetologia 56, 20592067.
Mancini, R and Hunt, M 2005. Current research in meat color. Meat Science 71, 100121.
Matte, J, Guay, F and Girard, C 2006. Folic acid and vitamin B12 in reproducing sows: new concepts. Canadian Journal of Animal Science 86, 197205.
Monin, G and Sellier, P 1985. Pork of low technological quality with a normal rate of muscle pH fall in the immediate post-mortem period: the case of the Hampshire breed. Meat Science 13, 4963.
Mou, D, Wang, J, Liu, H, Chen, Y, Che, L, Fang, Z, Xu, S, Lin, Y, Feng, B, Li, J and Wu, D 2017. Maternal methyl donor supplementation during gestation counteracts bisphenol A- induced oxidative stress in sows and offspring. Nutrition 45, 7684.
National Research Council (NRC) 2012. Nutrient requirements of swine, 11th revised edition. National Academy Press, Washington, DC, USA.
Oberdoerffer, S 2012. A conserved role for intragenic DNA methylation in alternative pre-mRNA splicing. Transcription 3, 106109.
Pederson, BA, Schroeder, JM, Parker, GE, Smith, MW, DePaoli-Roach, AA and Roach, PJ 2005. Glucose metabolism in mice lacking muscle glycogen synthase. Diabetes 54, 34663473.
Rubin, BS 2011. Bisphenol A: an endocrine disruptor with widespread exposure and multiple effects. The Journal of steroid biochemistry and molecular biology 127, 2734.
Schübeler, D 2015. Function and information content of DNA methylation. Nature 517, 321326.
Scheffler, T and Gerrard, D 2007. Mechanisms controlling pork quality development: The biochemistry controlling postmortem energy metabolism. Meat Science 77, 716.
Stockland, W and Blaylock, L 1974. Choline requirement of pregnant sows and gilts under restricted feeding conditions. Journal of Animal Science 39, 11131116.
Suman, SP and Joseph, P 2013. Myoglobin chemistry and meat color. Annual review of food science and technology 4, 7999.
van Wettere, W, Smits, R and Hughes, P 2013. Methyl donor supplementation of gestating sow diets improves pregnancy outcomes and litter size. Animal Production Science 53, 17.
Wang, J, Wu, Z, Li, D, Li, N, Dindot, SV, Satterfield, MC, Bazer, FW and Wu, G 2012. Nutrition, epigenetics, and metabolic syndrome. Antioxidants & Redox Signaling 17, 282301.
Zhang, C, Luo, J, Yu, B, Zheng, P, Huang, Z, Mao, X, He, J, Yu, J, Chen, J and Chen, D 2015. Dietary resveratrol supplementation improves meat quality of finishing pigs through changing muscle fiber characteristics and antioxidative status. Meat Science 102, 1521.

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