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Effects of curcumin on performance, antioxidation, intestinal barrier and mitochondrial function in ducks fed corn contaminated with ochratoxin A

  • D. Ruan (a1) (a2), W. C. Wang (a1), C. X. Lin (a2), A. M. Fouad (a2) (a3), W. Chen (a2), W. G. Xia (a2), S. Wang (a2), X. Luo (a2), W. H. Zhang (a4), S. J. Yan (a4), C. T. Zheng (a2) and L. Yang (a1)...


Curcumin has been attributed with antioxidant, anti-inflammatory, antibacterial activities, and has shown highly protective effects against enteropathogenic bacteria and mycotoxins. Ochratoxin A (OTA) is one of the major intestinal pathogenic mycotoxins. The possible effect of curcumin on the alleviation of enterotoxicity induced by OTA is unknown. The effects of dietary curcumin supplementation on OTA-induced oxidative stress, intestinal barrier and mitochondrial dysfunctions were examined in young ducks. A total of 540 mixed-sex 1-day-old White Pekin ducklings with initial BW (43.4±0.1 g) were randomly assigned into controls (fed only the basal diet), a group fed an OTA-contaminated diet (2 mg/kg feed), and a group fed the same OTA-contaminated feed plus 400 mg/kg of curcumin. Each treatment consisted of six replicates, each containing 30 ducklings and treatment lasted for 21 days. There was a significant decrease in average daily gain (ADG) and increased feed : gain caused by OTA (P<0.05); curcumin co-treatment prevented the decrease in BW and ADG compared with the OTA group (P<0.05). Histopathological and ultrastructural examination showed clear signs of enterotoxicity caused by OTA, but these changes were largely prevented by curcumin supplementation. Curcumin decreased the concentrations of interleukin-1β, tumor necrosis factor-α and malondialdehyde, and increased the activity of glutathione peroxidase induced by OTA in the jejunal mucosa of ducks (P<0.05). Additionally, curcumin increased jejunal mucosa occludin and tight junction protein 1 mRNA and protein levels, and decreased those of ρ-associated protein kinase 1 (P<0.05). Notably, curcumin inhibited the increased expression of apoptosis-related genes, and downregulated mitochondrial transcription factors A, B1 and B2 caused by OTA without any effects on RNA polymerase mitochondrial (P<0.05). These results indicated that curcumin could protect ducks from OTA-induced impairment of intestinal barrier function and mitochondrial integrity.


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Present address: Institute of Animal Science, Guangdong Academy of Agricultural Sciences, No.1 Dafeng First Street, Wushan Road, Tianhe District, Guangzhou 510640, China.



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Al-Anati, L, Essid, E, Stenius, U, Beuerlein, K, Schuh, K and Petzinger, E 2010. Differential cell sensitivity between OTA and LPS upon releasing TNF-α . Toxins 2, 12791299.
Asin-Cayuela, J and Gustafsson, CM 2007. Mitochondrial transcription and its regulation in mammalian cells. Trends in Biochemical Sciences 32, 111117.
Bradford, MM 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248254.
Chung, HL, Hwang, JB, Park, JJ and Kim, SG 2002. Expression of transforming growth factor beta1, transforming growth factor type I and II receptors, and TNF-alpha in the mucosa of the small intestine in infants with food protein-induced enterocolitis syndrome. Journal of Allergy and Clinical Immunology 109, 150154.
Dorta, DJ, Pigoso, AA, Mingatto, FE, Rodrigues, T, Prado, IMR, Helena, AFC, Uyemura, SA, Santos, AC and Curti, C 2005. The interaction of flavonoids with mitochondria: effects on energetic processes. Chemicro-Biological Interactions 152, 6778.
Du, LJ, Kim, JJ, Shen, JH and Dai, N 2016. Crosstalk between inflammation and ROCK/MLCK signaling pathways in gastrointestinal disorders with intestinal hyperpermeability. Gastroenterology Research and Practice 2016, 7374197.
Gowda, NKS, Ledoux, DR, Rottinghaus, GE, Bermudez, AJ and Chen, YC 2009. Antioxidant efficacy of curcuminoids from turmeric (Curcuma longa L.) powder in broiler chickens fed diets containing aflatoxin B1 . British Journal of Nutrition 102, 16291634.
Grenier, B and Applegate, TJ 2013. Modulation of intestinal functions following mycotoxin ingestion: meta-analysis of published experiments in animals. Toxins 5, 396430.
Hoehler, D and Marquardt, RR 1996. Influence of vitamins E and C on the toxic effects of ochratoxin A and T-2 toxin in chicks. Poultry Science 75, 15081515.
Ikeda, M, Ide, T, Fujino, T, Arai, S, Saku, K, Kakino, T, Tyynismaa, H, Yamasaki, T, Yamada, K, Kang, D, Suomalainen, A and Sunagawa, K 2015. Overexpression of TFAM or twinkle increases mtDNA copy number and facilitates cardioprotection associated with limited mitochondrial oxidative stress. PLoS ONE 10, 0119687.
Kang, D, Kim, SH and Hamasaki, N 2007. Mitochondrial transcription factor A (TFAM): roles in maintenance of mtDNA and cellular functions. Mitochondrion 7, 3944.
Kanki, T, Nakayama, H, Sasaki, N, Takio, K, Alam, TI, Hamasaki, N and Kang, D 2004. Mitochondrial nucleoid and transcription factor A. Annals of the New York Academy of Sciences 1011, 6168.
Kőszegi, T and Poór, M 2016. Ochratoxin A: molecular interactions, mechanisms of toxicity and prevention at the molecular level. Toxins 8, 111.
Li, JY, Yu, Y, Hao, J, Jin, H and Xu, HJ 1996. Determination of diamine oxidase activity in intestinal tissue and blood using spectrophotometry. Amino Acids & Biotic Resources 18, 2830.
Maresca, M, Mahfound, R, Pfohl-Leszkowicz, A and Fantini, J 2001. The mycotoxin ochratoxin A alters intestinal barrier and absorption functions but has no effect on chloride secretion. Toxicology and Applied Pharmacology 176, 5463.
McLaughlin, J, Padfield, PJ, Burt, JPH and O’Neill, CA 2004. Ochratoxin A increases permeability through tight junctions by removal of specific claudin isoforms. American Journal of Physiology Cell Physiology 287, 14121417.
Moghadamtousi, SZ, Kadir, HA, Hassandarvish, P, Tajik, H, Abubakar, S and Zandi, K 2014. A review on antibacterial, antiviral, and antifungal activity of curcumin. BioMed Research International 2014, 186864.
Muhammad, I, Sun, XQ, Wang, H, Li, W, Wang, XH, Cheng, P, Li, SH, Zhang, XY and Hamid, S 2017. Curcumin successfully inhibited the computationally identified CYP2A6 enzyme-mediated bioactivation of aflatoxin B1 in Arbor Acres broiler. Frontiers in Pharmacology 8, 143.
National Research Council (NRC) 1994. Nutrient requirements of Poultry, 9th revised edition. National Academy Press, Washington, DC, USA.
Poór, M, Veres, B, Jakus, PB, Antus, C, Montskó, G, Zrínyi, Z, Vladimir-Knežević, S, Petrik, J and Kőszegi, T 2014. Flavonoid diosmetin increases ATP levels in kidney cells and relieves ATP depleting effect of ochratoxin A. Journal of Photochemistry and Photobiology B: Biology 132, 19.
Prior, MG, Oneil, JB and Sisodia, CS 1980. Effects of ochratoxin A on growth response and residues in broilers. Poultry Science 59, 12541257.
Rainey, NE, Moustapha, A, Parker, R and Petit, PX 2017. Curcumin a multifaceted compound with hormetic behavior that mediates an intricate crosstalk between mitochondrial biogenesis, mitophagy, mitophagic death and apoptosis. Preprints 2017, 2017010039.
Rajput, N, Muhammad, N, Yan, R, Zhong, X and Wang, T 2013. Effect of dietary supplementation of curcumin on growth performance, intestinal morphology and nutrients utilization of broiler chicks. The Journal of Poultry Science 50, 4452.
Ranaldi, G, Mancini, E, Ferruzza, S, Sambuy, Y and Perozzi, G 2007. Effects of red wine on ochratoxin A toxicity in intestinal Caco-2/TC7 cells. Toxicology in Vitro 21, 204210.
Rao, VK, Ramana, MV, Girisham, S and Reddy, SM 2013. Culture media and factors influencing ochratoxin A production by two species of Penicillium isolated from poultry feeds. National Academy Science Letters 36, 101110.
Ringot, D, Chango, A, Schneider, Y and Larondelle, Y 2006. Toxicokinetics and toxicodynamics of ochratoxin A, an update. Chem-Biological Interactions 159, 1846.
Shen, G, Xu, C, Hu, R, Jain, MR, Gopalkrishnan, A, Nair, S, Huang, MT, Chan, JY and Kong, AN 2006. Modulation of nuclear factor E2-related 2-mediated gene expression in mice liver and small intestine by cancer chemopreventive agent curcumin. Molecular Cancer Therapeutics 5, 3951.
Śliżewska, K and Piotrowska, M 2014. Reduction of ochratoxin A in chicken feed using probiotic. Annals of Agricultural and Environmental Medicine 21, 676680.
Solcan, C, Pavel, G, Floristean, VC, Chiriac, ISB, Şlencu, BG and Solcan, G 2015. Effect of ochratoxin A on the intestinal mucosa and mucosa-associated lymphoid tissues in broiler chickens. Acta Veterinaria Hungarica 63, 3048.
Stoev, SD 2016. Food security and foodborne mycotoxicoses, risk assessment, preventive measures, and underestimated hazard of masked mycotoxins or joint mycotoxin interaction. Food Toxicology 9, 169199.
Suliman, HB, Carraway, MS, Welty-Wolf, KE, Whorton, AR and Piantadosi, CA 2003. Lipopolysaccharide stimulates mitochondrial biogenesis via activation of nuclear respiratory factor-1. Journal of Biological Chemistry 278, 4151041518.
Taha, R, Seidman, E, Mailhot, G, Boudreau, F, Gendron, F, Beaulieu, J, Menard, D, Delvin, E, Amre, D and Levy, E 2010. Oxidative stress and mitochondrial functions in the intestinal Caco-2/15 cell line. PLoS ONE 5, 11817.
Turner, JR 2009. Intestinal mucosal barrier function in health and disease. Nature Reviews Immunology 9, 799809.
Venditti, P, Stefano, LD and Meo, SD 2013. Mitochondrial metabolism of reactive oxygen species. Mitochondrion 13, 7182.
Wang, N, Wang, G, Hao, JX, Ma, JJ, Wang, Y, Jiang, XY and Jiang, HQ 2012. Curcumin ameliorates hydrogen peroxide-induced epithelial barrier disruption by upregulating heme oxygenase-1 expression in human intestinal epithelial cells. Digestive Diseases and Sciences 57, 17921801.
Wei, YH, Lu, TN and Wei, RD 1985. Effect of ochratoxin A on rat liver mitochondrial respiration and oxidative phosphorylation. Toxicology 36, 119123.
Zhong, L 2008. Effects of moldy feed by Aspergillus ochraceus on Cherry Valley male duck. Master thesis, South China Agricultural University, Guangzhou, China.


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Effects of curcumin on performance, antioxidation, intestinal barrier and mitochondrial function in ducks fed corn contaminated with ochratoxin A

  • D. Ruan (a1) (a2), W. C. Wang (a1), C. X. Lin (a2), A. M. Fouad (a2) (a3), W. Chen (a2), W. G. Xia (a2), S. Wang (a2), X. Luo (a2), W. H. Zhang (a4), S. J. Yan (a4), C. T. Zheng (a2) and L. Yang (a1)...


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