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Effect of inorganic phosphate supplementation on egg production in Hy-Line Brown layers fed 2000 FTU/kg phytase

  • X. Cheng (a1), J. K. Yan (a1), W. Q. Sun (a1), Z. Y. Chen (a2), S. R. Wu (a1), Z. Z. Ren (a1) and X. J. Yang (a1)...

Abstract

Phytase has long been used to decrease the inorganic phosphorus (Pi) input in poultry diet. The current study was conducted to investigate the effects of Pi supplementation on laying performance, egg quality and phosphate–calcium metabolism in Hy-Line Brown laying hens fed phytase. Layers (n = 504, 29 weeks old) were randomly assigned to seven treatments with six replicates of 12 birds. The corn–soybean meal-based diet contained 0.12% non-phytate phosphorus (nPP), 3.8% calcium, 2415 IU/kg vitamin D3 and 2000 FTU/kg phytase. Inorganic phosphorus (in the form of mono-dicalcium phosphate) was added into the basal diet to construct seven experimental diets; the final dietary nPP levels were 0.12%, 0.17%, 0.22%, 0.27%, 0.32%, 0.37% and 0.42%. The feeding trial lasted 12 weeks (hens from 29 to 40 weeks of age). Laying performance (housed laying rate, egg weight, egg mass, daily feed intake and feed conversion ratio) was weekly calculated. Egg quality (egg shape index, shell strength, shell thickness, albumen height, yolk colour and Haugh units), serum parameters (calcium, phosphorus, parathyroid hormone, calcitonin and 1,25-dihydroxyvitamin D), tibia quality (breaking strength, and calcium, phosphorus and ash contents), intestinal gene expression (type IIb sodium-dependent phosphate cotransporter, NaPi-IIb) and phosphorus excretion were determined at the end of the trial. No differences were observed on laying performance, egg quality, serum parameters and tibia quality. Hens fed 0.17% nPP had increased (P < 0.01) duodenum NaPi-IIb expression compared to all other treatments. Phosphorus excretion linearly increased with an increase in dietary nPP (phosphorus excretion = 1.7916 × nPP + 0.2157; R2 = 0.9609, P = 0.001). In conclusion, corn–soybean meal-based diets containing 0.12% nPP, 3.8% calcium, 2415 IU/kg vitamin D3 and 2000 FTU/kg phytase would meet the requirements for egg production in Hy-Line Brown laying hens (29 to 40 weeks of age).

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These two authors contributed equally to this work.

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Ahmadi, H and Rodehutscord, M 2012. A meta-analysis of responses to dietary nonphytate phosphorus and phytase in laying hens. Poultry Science 91, 20722078.
Bai, X, Miao, D, Li, J, Goltzman, D and Karaplis, AC 2004. Transgenic mice overexpressing human fibroblast growth factor 23 (R176Q) delineate a putative role for parathyroid hormone in renal phosphate wasting disorders. Endocrinology 145, 52695279.
Bar, A, Razaphkovsky, V and Vax, E 2002. Re-evaluation of calcium and phosphorus requirements in aged laying hens. British Poultry Science 43, 261269.
Bikker, P, Spek, JW, Van-Emous, RA and Van-Krimpen, MM 2016. Precaecal phosphorus digestibility of inorganic phosphate sources in male broilers. British Poultry Science 57, 810817.
Boling, SD, Douglas, MW, Johnson, ML, Wang, X, Parsons, CM, Koelkebeck, KW and Zimmerman, RA 2000a. The effects of dietary available phosphorus levels and phytase on performance of young and older laying hens. Poultry Science 79, 224230.
Boling, SD, Douglas, MW, Shirley, RB, Parsons, CM and Koelkebeck, KW 2000b. The effects of various dietary levels of phytase and available phosphorus on performance of laying hens. Poultry Science 79, 535538.
Boorman, KN and Gunaratne, SP 2001. Dietary phosphorus supply, egg-shell deposition and plasma inorganic phosphorus in laying hens. British Poultry Science 42, 8191.
Cordell, D, Drangert, JO and White, S 2009. The story of phosphorus: global food security and food for thought. Global Environmental Change 19, 292305.
Fukumoto, S 2014. Phosphate metabolism and vitamin D. BoneKEy Reports 3, 497. doi: 10.1038/bonekey.2013.231.
Gordon, RW and Roland, DA 1997. Performance of commercial laying hens fed various phosphorus levels, with and without supplemental phytase. Poultry Science 76, 11721177.
Guo, YQ, Tu, Y, Zhang, NF, Liu, GH, Tang, DF, Wang, ZY, Zhong, H, Li, YJ and Ma, L 2018. Current situation and optimization strategy of phosphorus recommendation level and phosphate application of feed in China. Scientia Agricultura Sinica 51, 581592.
Humer, E, Schwarz, C and Schedle, K 2015. Phytate in pig and poultry nutrition. Journal of Animal Physiology and Animal Nutrition 99, 605625.
Huttunen, MM, Pietila, PE, Viljakainen, HT and Lamberg-Allardt, CJ 2006. Prolonged increase in dietary phosphate intake alters bone mineralization in adult male rats. The Journal of Nutritional Biochemistry 17, 479484.
Jing, M, Zhao, S, Rogiewicz, A, Slominski, BA and House, JD 2018. Assessment of the minimal available phosphorus needs of laying hens: implications for phosphorus management strategies. Poultry Science 97, 24002410.
Keshavarz, K 2003. The effect of different levels of nonphytate phosphorus with and without phytase on the performance of four strains of laying hens. Poultry Science 82, 7191.
Kim, WK, Donalson, LM, Bloomfield, SA, Hogan, HA, Kubena, LF, Nisbet, DJ and Ricke, SC 2007. Molt performance and bone density of cortical, medullary, and cancellous bone in laying hens during feed restriction or alfalfa-based feed molt. Poultry Science 86, 18211830.
Lei, QB, Shi, LX, Zhang, KY, Ding, XM, Bai, SP and Liu, YG 2011. Effect of reduced energy, protein and entire substitution of inorganic phosphorus by phytase on performance and bone mineralisation of laying hens. British Poultry Science 52, 202213.
Li, P, Wang, R, Jiao, H, Wang, X, Zhao, J and Lin, H 2018. Effects of dietary phosphorus level on the expression of calcium and phosphorus transporters in laying hens. Frontiers in Physiology 9, 627. doi: 10.3389/fphys.2018.00627.
Liao, XD, Suo, HQ, Lu, L, Hu, YX, Zhang, LY and Luo, XG 2017. Effects of sodium, 1,25-dihydroxyvitamin D3 and parathyroid hormone fragment on inorganic P absorption and Type IIb sodium-phosphate cotransporter expression in ligated duodenal loops of broilers. Poultry Science 96, 23442350.
Liu, Y, Guo, W, Pu, Z, Li, X, Lei, X, Yao, J and Yang, X 2016. Developmental changes of Insulin-like growth factors in the liver and muscle of chick embryos. Poultry Science 95, 13961402.
Mirakzehi, MT, Kermanshahi, H, Golian, A and Raji, AR 2013. The effects of dietary 1, 25-dihydroxycholecalciferol and hydroalcoholic extract of Withania somnifera root on bone mineralisation, strength and histological characteristics in broiler chickens. British Poultry Science 54, 789800.
Nie, W, Yang, Y, Yuan, J, Wang, Z and Guo, Y 2013. Effect of dietary nonphytate phosphorus on laying performance and small intestinal epithelial phosphate transporter expression in Dwarf pink-shell laying hens. Journal of Animal Science Biotechnology 4, 34. doi: 10.1186/2049-1891-4-34.
National Research Council (NRC) 1994. Nutrient requirements of poultry, 9th revised edition. National Academies Press, Washington, DC, USA.
Ren, Z, Bütz, DE, Sand, JM and Cook, ME 2017a. Maternally-derived anti-fibroblast growth factor 23 antibody as new tool to reduce phosphorus requirement of chicks. Poultry Science 96, 878885.
Ren, Z, Bütz, DE, Wahhab, AN, Piepenburg, AJ and Cook, ME 2017b. Additive effects of fibroblast growth factor 23 neutralization and dietary phytase on chick calcium and phosphorus metabolism. Poultry Science 96, 11671173.
Ren, Z, Ebrahimi, M, Butz, DE, Sand, JM, Zhang, K and Cook, ME 2017c. Antibody to fibroblast growth factor 23-peptide reduces excreta phosphorus of laying hens. Poultry Science 96, 127134.
Ren, Z, Jiang, S, Zeng, Q, Ding, X, Bai, S, Wang, J, Luo, Y, Su, Z, Xuan, Y, Yao, B, Cisneros, F and Zhang, K 2016. Effect of dietary canthaxanthin and 25-hydroxycholecalciferol supplementation on the performance of duck breeders under two different vitamin regimens. Journal of Animal Science and Biotechnology 7, 2. doi: 10.1186/s40104-016-0062-3.
Ren, Z, Piepenburg, AJ, Yang, X and Cook, ME 2019. Effect of anti-fibroblast growth factor 23 antibody on phosphate and calcium metabolism in adenine gavaged laying hens. Poultry Science 98, 48964900.
Saddoris, KL, Fleet, JC and Radcliffe, JS 2010. Sodium-dependent phosphate uptake in the jejunum is post-transcriptionally regulated in pigs fed a low-phosphorus diet and is independent of dietary calcium concentration. Journal of Nutrition 140, 731736.
Scott, TA, Kampen, R and Silversides, FG 1999. The effect of phosphorus, phytase enzyme, and calcium on the performance of layers fed corn-based diets. Poultry Science 78, 17421749.
Silversides, FG, Korver, DR and Budgell, KL 2006. Effect of strain of layer and age at photostimulation on egg production, egg quality, and bone strength. Poultry Science 85, 11361144.
Snow, JL, Douglas, MW, Koelkebeck, KW, Batal, AB, Persia, ME, Biggs, PE and Parsons, CM 2004. Minimum phosphorus requirement of one-cycle and two-cycle (molted) hens. Poultry Science 83, 917924.
Usayran, N, Farran, MT, Awadallah, HH, Al-Hawi, IR, Asmar, RJ and Ashkarian, VM 2001. Effects of added dietary fat and phosphorus on the performance and egg quality of laying hens subjected to a constant high environmental temperature. Poultry Science 80, 16951701.
Van der Klis, JD, Versteegh, HA, Simons, PC and Kies, AK 1997. The efficacy of phytase in corn-soybean meal-based diets for laying hens. Poultry Science 76, 15351542.
Viveros, A, Brenes, A, Arija, I and Centeno, C 2002. Effects of microbial phytase supplementation on mineral utilization and serum enzyme activities in broiler chicks fed different levels of phosphorus. Poultry Science 81, 11721183.
Vuuren, DPV, Bouwman, AF and Beusen, AHW 2010. Phosphorus demand for the 1970–2100 period: a scenario analysis of resource depletion. Global Environmental Change 20, 428439.
Wagner, CA 2007. Novel insights into the regulation of systemic phosphate homeostasis and renal phosphate excretion. Journal of Nephrology 20, 130134.
Wang, S, Tang, CH, Zhang, JM and Wang, XQ 2013. The effect of dietary supplementation with phytase transgenic maize and different concentrations of non-phytate phosphorus on the performance of laying hens. British Poultry Science 54, 466470.
Yan, F, Angel, R and Ashwell, CM 2007. Characterization of the chicken small intestine type IIb sodium phosphate cotransporter. Poultry Science 86, 6776.

Keywords

Effect of inorganic phosphate supplementation on egg production in Hy-Line Brown layers fed 2000 FTU/kg phytase

  • X. Cheng (a1), J. K. Yan (a1), W. Q. Sun (a1), Z. Y. Chen (a2), S. R. Wu (a1), Z. Z. Ren (a1) and X. J. Yang (a1)...

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