Skip to main content Accessibility help
×
Home

Differential mesenteric fat deposition in bovines fed on silage or concentrate is independent of glycerol membrane permeability

  • A. P. Martins (a1), P. A. Lopes (a2), A. S. H. Costa (a2), S. V. Martins (a2), N. C. Santos (a3), J. A. M. Prates (a2), T. F. Moura (a1) and G. Soveral (a1) (a4)...

Abstract

In the meat industry, the manipulation of fat deposition in cattle is of pivotal importance to improve production efficiency, carcass composition and ultimately meat quality. There is an increasing interest in the identification of key factors and molecular mechanisms responsible for the development of specific fat depots. This study aimed at elucidating the influence of breed and diet on adipose tissue membrane permeability and fluidity and their interplay on fat deposition in bovines. Two Portuguese autochthonous breeds, Alentejana and Barrosã, recognized as late- and early-maturing breeds, respectively, were chosen to examine the effects of breed and diet on fat deposition and on adipose membrane composition and permeability. Twenty-four male bovines from these breeds were fed on silage-based or concentrate-based diets for 11 months. Animals were slaughtered to determine their live slaughter and hot carcass weights, as well as weights of subcutaneous and visceral adipose depots. Mesenteric fat depots were excised and used to isolate adipocyte membrane vesicles where cholesterol content, fatty acid profile as well as permeability and fluidity were determined. Total accumulation of neither subcutaneous nor visceral fat was influenced by breed. In contrast, mesenteric and omental fat depots weights were higher in concentrate-fed bulls relative to silage-fed animals. Membrane fluidity and permeability to water and glycerol in mesenteric adipose tissue were found to be independent of breed and diet. Moreover, the deposition of cholesterol and unsaturated fatty acids, which may influence membrane properties, were unchanged among experimental groups. Adipose membrane lipids from the mesenteric fat depot of ruminants were rich in saturated fatty acids, and unaffected by polyunsaturated fatty acids dietary levels. Our results provide evidence against the involvement of cellular membrane permeability to glycerol on fat accumulation in mesenteric fat tissue of concentrate-fed bovines, which is consistent with the unchanged membrane lipid profile found among experimental groups.

Copyright

Corresponding author

Footnotes

Hide All
a

These authors have contributed equally to this article.

Footnotes

References

Hide All
Alfaia, CMM, Castro, MLF, Martins, SIV, Portugal, APV, Alves, SPA, Fontes, CMGA, Bessa, RJB, Prates, JAM 2007. Effect of slaughter season on fatty acid composition, conjugated linoleic acid isomers and nutritional value of intramuscular fat in Barrosã-PDO veal. Meat Science 75, 4452.
Alfaia, CPM, Alves, SP, Martins, SIV, Costa, ASH, Fontes, CMGA, Lemos, JPC, Bessa, RJB, Prates, JAM 2009. Effect of the feeding system on intramuscular fatty acids and conjugated linoleic acid isomers of beef cattle, with emphasis on their nutritional value and discriminatory ability. Food Chemistry 114, 939946.
Alves, SP, Bessa, RJ 2009. Comparison of two gas–liquid chromatograph columns for the analysis of fatty acids in ruminant meat. Journal of Chromatography A 1216, 51305139.
Azain, MJ 2004. Role of fatty acids in adipocyte growth and development. Journal of Animal Science 82, 916924.
Beja-Pereira, A, Alexandrino, P, Bessa, I, Carretero, Y, Dunner, S, Ferrand, N, Jordana, J, Laloe, D, Moazami-Goudarzi, K, Sanchez, A, Canon, J 2003. Genetic characterization of southwestern European bovine breeds: a historical and biogeographical reassessment with a set of 16 microsatellites. Journal of Heredity 94, 243250.
Bhattacharya, A, Banu, J, Rahman, M, Causey, J, Fernandes, G 2006. Biological effects of conjugated linoleic acids in health and disease. Journal of Nutritional Biochemistry 17, 789810.
Bradford, MM 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72, 248254.
Christie, WW, Dobson, G, Adlof, RO 2007. A practical guide to the isolation, analysis and identification of conjugated linoleic acid. Lipids 42, 10731084.
Clandinin, MT, Field, CJ, Hargreaves, K, Morson, L, Zsigmond, E 1985. Role of diet fat in subcellular structure and function. Canadian Journal of Physiology and Pharmacology 63, 546556.
da Silva, MF, Lemos, JPC, Monteiro, LS, Portugal, AV 1998. Studies on growth and form: multivariate analysis of distribution of muscle and fat in Portuguese cattle breeds. Livestock Production Science 55, 261271.
Daniel, ZCTR, Wynn, RJ, Salter, AM, Buttery, PJ 2004. Differing effects of forage and concentrate diets on the oleic acid and conjugated linoleic acid content of sheep tissues: the role of stearoyl-CoA desaturase. Journal of Animal Science 82, 747758.
De Smet, S, Raes, K, Demeyer, D 2004. Meat fatty acid composition as affected by fatness and genetic factors: a review. Animal Research 53, 8198.
Dix, JA, Ausiello, DA, Jung, CY, Verkman, AS 1985. Target analysis studies of red cell water and urea transport. Biochimica et Biophysica Acta 821, 243252.
Enser, M, Hallett, K, Hewitt, B, Fursey, GAJ, Wood, JD 1996. Fatty acid content and composition of English beef, lamb and pork at retail. Meat Science 42, 443456.
Field, CJ, Clandinin, MT 1984. Modulation of adipose-tissue fat composition by diet – a review. Nutrition Research 4, 743755.
Field, CJ, Ryan, EA, Thomson, ABR, Clandinin, MT 1988. Dietary-fat and the diabetic state alter insulin binding and the fatty acyl composition of the adipocyte plasma-membrane. Biochemical Journal 253, 417424.
Hocquette, JF, Gondret, F, Baeza, E, Medale, F, Jurie, C, Pethick, DW 2010. Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers. Animal 4, 303319.
Jambrenghi, AC, Paglialonga, G, Gnoni, A, Zanotti, F, Giannico, F, Vonghia, G, Gnoni, GV 2007. Changes in lipid composition and lipogenic enzyme activities in liver of lambs fed omega-6 polyunsaturated fatty acids. Comparative Biochemistry and Physiology B – Biochemistry & Molecular Biology 147, 498503.
Jenkins, TC 1994. Regulation of lipid-metabolism in the rumen. The Journal of Nutrition 124, S1372S1376.
Keating, AF, Kennelly, JJ, Zhao, FQ 2006. Characterization and regulation of the bovine stearoyl-CoA desaturase gene promoter. Biochemical and Biophysical Research Communications 344, 233240.
Lakowicz, JR 1999. Principles of fluorescence spectroscopy. Kluwer Academic/Plenum, New York.
Lande, MB, Donovan, JM, Zeidel, ML 1995. The relationship between membrane fluidity and permeabilities to water, solutes, ammonia, and protons. Journal of General Physiology 106, 6784.
Lock, AL, Corl, BA, Barbano, DM, Bauman, DE, Ip, C 2004. The anticarcinogenic effect of trans-11 18:1 is dependent on its conversion to cis-9, trans-11 CLA by delta 9-desaturase in rats. The Journal of Nutrition 134, 26982704.
Martins, AP, Lopes, PA, Martins, SV, Madeira, A, Santos, NC, Prates, JA, Moura, TF, Soveral, G 2010. Conjugated linoleic acid reduces permeability and fluidity of adipose plasma membranes from obese Zucker rats. Biochemical and Biophysical Research Communications 398, 199204.
Mukesh, M, Bionaz, M, Graugnard, DE, Drackley, JK, Loor, JJ 2010. Adipose tissue depots of Holstein cows are immune responsive: inflammatory gene expression in vitro. Domestic Animal Endocrinology 38, 168178.
Naeemi, ED, Ahmad, N, al-Sharrah, TK, Behbahani, M 1995. Rapid and simple method for determination of cholesterol in processed food. Journal of AOAC International 78, 15221525.
Novakofski, J 2004. Adipogenesis: usefulness of in vitro and in vivo experimental models. Journal of Animal Science 82, 905915.
Onuki, Y, Hagiwara, C, Sugibayashi, K, Takayama, K 2008. Specific effect of polyunsaturated fatty acids on the cholesterol-poor membrane domain in a model membrane. Chemical & Pharmaceutical Bulletin 56, 11031109.
Pond, CM 1999. Physiological specialisation of adipose tissue. Progress in Lipid Research 38, 225248.
Raes, K, De Smet, S, Demeyer, D 2001. Effect of double-muscling in Belgian Blue young bulls on the intramuscular fatty acid composition with emphasis on conjugated linoleic acid and polyunsaturated fatty acids. Animal Science 73, 253260.
Reis, C, Navas, D, Pereira, M, Cravador, A 2001. Growth hormone ALUI polymorphism analysis in eight Portuguese bovine breeds. Archivos de Zootecnia 50, 4148.
Sarkkinen, ES, Agren, JJ, Ahola, I, Ovaskainen, ML, Uusitupa, MIJ 1994. Fatty-acid composition of serum-cholesterol esters, and erythrocyte and platelet membranes as indicators of long-term adherence to fat-modified diets. American Journal of Clinical Nutrition 59, 364370.
Simões, JA, Mendes, I 2003. Distribution of tissues in carcasses at the same proportion of total fat in Portuguese cattle breeds. Animal Research 52, 287298.
Soveral, G, Martins, AP, Martins, SV, Lopes, PA, Alfaia, CM, Prates, JA, Moura, TF 2009. Effect of dietary conjugated linoleic acid isomers on water and glycerol permeability of kidney membranes. Biochemical and Biophysical Research Communications 383, 108112.
Stillwell, W, Wassall, SR 2003. Docosahexaenoic acid: membrane properties of a unique fatty acid. Chemistry and Physics of Lipids 126, 127.
Stubbs, CD, Smith, AD 1984. The modification of mammalian membrane poly-unsaturated fatty-acid composition in relation to membrane fluidity and function. Biochimica et Biophysica Acta 779, 89137.
van Heeswijk, MP, van Os, CH 1986. Osmotic water permeabilities of brush border and basolateral membrane vesicles from rat renal cortex and small intestine. Journal of Membrane Biology 92, 183193.
Vernon, RG, Houseknecht, KL 1991. Adipose tissue: beyond an energy reserve. In Ruminant physiology: digestion, metabolism, growth and reproduction (ed. PB Cronjé), pp. 171186. CAB International, London.
Wachira, AM, Sinclair, LA, Wilkinson, RG, Enser, M, Wood, JD, Fisher, AV 2002. Effects of dietary fat source and breed on the carcass composition, n-3 polyunsaturated fatty acid and conjugated linoleic acid content of sheep meat and adipose tissue. British Journal of Nutrition 88, 697709.

Keywords

Differential mesenteric fat deposition in bovines fed on silage or concentrate is independent of glycerol membrane permeability

  • A. P. Martins (a1), P. A. Lopes (a2), A. S. H. Costa (a2), S. V. Martins (a2), N. C. Santos (a3), J. A. M. Prates (a2), T. F. Moura (a1) and G. Soveral (a1) (a4)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed