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D1, but not D2, dopamine receptor regulates steroid levels during the final stages of pikeperch gametogenesis

Published online by Cambridge University Press:  22 April 2018

J. Roche ,
D. Żarski ,
A. Khendek ,
I. Ben Ammar Open the ORCID record for I. Ben Ammar [Opens in a new window] ,
C. Broquard ,
A. Depp ,
Y. Ledoré ,
T. Policar ,
P. Fontaine  and
S. Milla
J. Roche
Affiliation:
UR AFPA, USC INRA 340, Université de Lorraine, Boulevard des Aiguillettes, 54506, Vandoeuvre-lès-Nancy, France
D. Żarski
Affiliation:
UR AFPA, USC INRA 340, Université de Lorraine, Boulevard des Aiguillettes, 54506, Vandoeuvre-lès-Nancy, France
A. Khendek
Affiliation:
UR AFPA, USC INRA 340, Université de Lorraine, Boulevard des Aiguillettes, 54506, Vandoeuvre-lès-Nancy, France
I. Ben Ammar
Affiliation:
UR AFPA, USC INRA 340, Université de Lorraine, Boulevard des Aiguillettes, 54506, Vandoeuvre-lès-Nancy, France
C. Broquard
Affiliation:
UR AFPA, USC INRA 340, Université de Lorraine, Boulevard des Aiguillettes, 54506, Vandoeuvre-lès-Nancy, France
A. Depp
Affiliation:
UR AFPA, USC INRA 340, Université de Lorraine, Boulevard des Aiguillettes, 54506, Vandoeuvre-lès-Nancy, France
Y. Ledoré
Affiliation:
UR AFPA, USC INRA 340, Université de Lorraine, Boulevard des Aiguillettes, 54506, Vandoeuvre-lès-Nancy, France
T. Policar
Affiliation:
South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia, Zatisi 728/II, 389 25, Vodnany, Czech Republic
P. Fontaine
Affiliation:
UR AFPA, USC INRA 340, Université de Lorraine, Boulevard des Aiguillettes, 54506, Vandoeuvre-lès-Nancy, France
S. Milla*
Affiliation:
UR AFPA, USC INRA 340, Université de Lorraine, Boulevard des Aiguillettes, 54506, Vandoeuvre-lès-Nancy, France
*
†E-mail: sylvain.milla@univ-lorraine.fr
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Abstract

In pikeperch, Sander lucioperca, aquaculture hormonal treatment is usually applied to synchronize ovulation. However, the effect of dopamine (DA) receptor antagonists, in particular those blocking the D1 DA receptors, remains unknown. Thus, the aim of the present study was to investigate and compare the effects of D1 and D2 DA receptor antagonists on the sex-steroid production and reproductive performance of the species. Two experiments were performed during which mature pikeperch females were injected with different molecules: NaCl 0.9% (negative control) or human chorionic gonadotropin 500 IU/kg (positive control) in both experiments, metoclopramide (a D2 receptor antagonist; 4 mg/kg or 20 mg/kg) or SCH23390 (a D1 receptor antagonist; 0.8 mg/kg or 4 mg/kg) alone (experiment 1) or in combination with a salmon gonadotropin-releasing hormone analogue (sGnRHa at 25 µg/kg; experiment 2). In experiment 2, fish were also injected with sGnRHa (25 µg/kg) as positive control. Samplings of oocytes and blood were performed on the day of injection and after 24 h (both experiments), after 48 h (experiment 2) and at the time of ovulation (both experiments). In non-ovulating fish, samplings were performed 7 days (experiment 1) or 14 days (experiment 2) after injection. In experiment 2, various zootechnical parameters of fertilized eggs were recorded (survival, hatching and malformation rates). The two antagonists alone were ineffective in inducing the final stages and regulating sex-steroid (testosterone, 11 ketotestosterone, 17β estradiol and 17,20β-dihydroxy-4-pregnen-3-one) production. When administered with sGnRHa, both SCH23390 and metoclopramide induced the final stages. However, only SCH23390 stimulated testosterone (4 mg/kg) and 17β estradiol (0.8 mg/kg) production compared with sGnRHa alone. None of the treatments affected the survival, hatching or malformation rates. This is the first report suggesting that in pikeperch the D1, but not the D2, DA receptor antagonist would be involved in the testosterone and 17β estradiol production as a potentiator of the sGnRHa effect.

Keywords

pikeperchovulationdopamineantagonistsex steroid
Type
Research Article
Information
animal , Volume 12 , Issue 12 , December 2018 , pp. 2587 - 2597
Copyright
© The Animal Consortium 2018 

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Footnotes

a

Present address: Department of Ichthyology, Faculty of Environmental Sciences, University of Warmia and Mazury, ul. Oczapowskiego 2, 10-719, Olsztyn, Poland.

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