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Conjugated linolenic acid (CLnA), conjugated linoleic acid (CLA) and other biohydrogenation intermediates in plasma and milk fat of cows fed raw or extruded linseed

  • F. Akraim (a1) (a2), M. C. Nicot (a1), P. Juaneda (a3) and F. Enjalbert (a1)

Abstract

Thirty lactating dairy cows were used in a 3 × 3 Latin-square design to investigate the effects of a raw or extruded blend of linseed and wheat bran (70:30) on plasma and milk fatty-acids (FA). Linseed diets, containing 16.6% linseed blend on a dry-matter basis, decreased milk yield and protein percentage. They decreased the proportions of FA with less than 18 carbons in plasma and milk and resulted in cis-9, cis-12, cis-15 18:3 proportions that were more than three and four times higher in plasma and milk, respectively, whereas cis-9, cis-12 18:2 proportions were decreased by 10–15%. The cis-9, trans-11, cis-15 18:3 isomer of conjugated linolenic acid was not detected in the milk of control cows, but was over 0.15% of total FA in the milk fat of linseed-supplemented cows. Similarly, linseed increased plasma and milk proportions of all biohydrogenation (BH) intermediates in plasma and milk, including the main isomer of conjugated linoleic acid cis-9, trans-11 18:2, except trans-4 18:1 and cis-11, trans-15 18:2 in plasma lipids. In milk fat, compared with raw linseed, extruded linseed further reduced 6:0–16:0 even-chain FA, did not significantly affect the proportions of 18:0, cis-9 18:1 and cis-9, cis-12 18:2, tended to increase cis-9, cis-12, cis-15 18:3, and resulted in an additional increase in the proportions of most BH intermediates. It was concluded that linseed addition can improve the proportion of conjugated linoleic and linolenic acids, and that extrusion further increases the proportions of intermediates of ruminal BH in milk fat.

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References

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Association of Official Analytical Chemists. 1996. Animal Feed. Official methods of analysis, 16th edition. AOAC, Gaithersburg, MA.
Bauman, DE, Corl, BA, Baumgard, LH, Griinari, JM 2001. Conjugated linoleic acid (CLA) and the dairy cow. In Recent advances in animal nutrition (ed. PC Garnsworthy and J Wiseman), pp. 221250. Nottingham University Press, Nottingham, UK.
Bauman DE, Perfield JW and Lock AL. 2004. Effect of trans fatty acids on milk fat and their impact on human health. Proceedings of the 19th Southwest Nutrition and Management Conference, University of Arizona, USA, pp. 41–52.
Baumgard, LH, Corl, BA, Dwyer, DA, Saebo, A, Bauman, DE 2000. Identification of the conjugated linoleic acid isomer that inhibits milk fat synthesis. American Journal of Physiology, Regulatory, Integrative and Compared Physiology 278, R179R184.
Bayourthe, C, Enjabert, F, Moncoulon, R 2000. Effects of different forms of canola oil fatty acids plus canola meals on milk composition and physical properties of butter. Journal of Dairy Science 83, 690697.
Cant, JP, DePeters, EJ, Baldwin, RL 1993. Mammary amino-acid utilization in dairy cows fed fat and its relationship to milk protein depression. Journal of Dairy Science 76, 762774.
Chapoutot, P, Sauvant, D 1997. Nutritive value of raw and extruded pea-rapeseed blends for ruminants. Animal Feed Science and Technology 65, 5977.
Chouinard, PY, Lévesque, J, Girard, V, Brisson, J 1997. Dietary soybeans extruded at different temperatures: milk composition and in situ fatty reactions. Journal of Dairy Science 80, 29132925.
Chouinard, PY, Corneau, L, Bulter, WR, Chilliard, Y, Drackley, JK, Bauman, DE 2001. Effect of dietary lipid source on conjugated linoleic acid concentrations in milk fat. Journal of Dairy Science 84, 680690.
Clapperton, JL, Banks, W 1985. Factors affecting the yield of milk and its constituents, particularly fatty acids, when dairy cows consume diets containing adding fat. Journal of the Science of Food and Agriculture 36, 12051211.
Destaillats, F, Trottier, JP, Garro Galvez, JM, Angers, P 2005. Analysis of α-linolenic acid biohydrogenation intermediates in milk fat with emphasis on conjugated linolenic acids (CLnA). Journal of Dairy Science 88, 32313239.
Duckett, SK, Andrae, JG, Owens, FN 2002. Effect of high-oil corn or added corn oil on ruminal biohydrogenation of fatty acids and conjugated linoleic acid formation in beef steers fed finishing diets. Journal of Animal Science 80, 33533360.
Federation of Animal Sciences Societies 1999. Guide for the care and use of agricultural animals in agricultural research and teaching. FASS, Savoy, IL, USA.
Folch, J, Lees, M, Stanley, GHS 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497509.
Gonthier, C, Mustafa, AF, Ouellet, DR, Chouinard, PY, Berthiaume, R, Petit, HV 2005. Feeding micronized and extruded flaxseed to dairy cows: effects on blood parameters and milk fatty acid composition. Journal of Dairy Science 88, 748756.
Griinari, JM, Corl, BA, Lacy, SH, Chouinard, PY, Nurmela, KV, Bauman, DE 2000. Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by delta(9)-desaturase. Journal of Nutrition 130, 22852291.
Grummer, RR 1991. Effect of feed on the composition of milk fat. Journal of Dairy Science 74, 32443257.
Harfoot, CG, Hazlewood, GP 1988. Lipid metabolism in the rumen. In The rumen microbial ecosystem (ed. PN Hobson), pp. 285322. Elsevier Science Publishers, London, UK.
Igarashi, M, Miyazawa, T 2000. Newly recognized cytotoxic effect of conjugated trienoic fatty acids on cultured human tumor cells. Cancer Letter 148, 173179.
Kelsey, JA, Corl, BA, Collier, RJ, Bauman, DE 2003. The effect of breed, parity, and stage of lactation on conjugated linoleic acid (CLA) in milk fat from dairy cows. Journal of Dairy Science 86, 25882597.
Kennelly, JJ 1996. The fatty acid composition of milk fat as influenced by feeding oil seed. Animal Feed Science and Technology 60, 137152.
LeDoux, M, Rouzeau, A, Bas, P, Sauvant, D 2002. Occurrence of trans-18:1 fatty acid isomers in goat milk: effect of two dietary regimens. Journal of Dairy Science 85, 190197.
Lock, AL, Garnsworthy, PC 2002. Independent effects of dietary linoleic and linolenic acids on the conjugated linoleic acid of cow’s milk. Animal Science 74, 163175.
Lock, AL, Tyburczy, C, Dwyer, DA, Harvatine, KJ, Destaillats, F, Mouloungi, Z, Candy, L, Bauman, DE 2007. Trans-10 octadecenoic acid does not reduce milk fat synthesis in dairy cows. Journal of Nutrition 137, 7176.
Loor, JJ, Bandara, ABPA, Herbein, JH 2002. Characterization of 18:1 and 18:2 isomers produced during microbial biohydrogenation of unsaturated fatty acids from canola and soya bean oil in rumen of lactating cows. Journal of Animal Physiology and Animal Nutrition 86, 422432.
Loor, JJ, Ferlay, A, Ollier, A, Doreau, M, Chilliard, Y 2005. Relationship among trans and conjugated fatty acids and bovine milk fat yield due to dietary concentrate and linseed oil. Journal of Dairy Science 88, 726740.
Loor, JJ, Ueda, K, Ferlay, A, Chilliard, Y, Doreau, M 2004. Biohydrogenation, duodenal flow, and intestinal digestibility of trans fatty acids and conjugated linoleic acids in response to dietary forage:concentrate ratio and linseed oil in dairy cows. Journal of Dairy Science 87, 24722485.
Mosley, EE, Powell, GL, Riley, MB, Jenkins, TC 2002. Microbial biohydrogenation of oleic acid to trans isomers in vitro. Journal of Lipid Research 43, 290296.
Park, PW, Goins, RE 1994. In situ preparation of fatty acid methyl esters for analysis of fatty acid composition in foods. Journal of Food Science 59, 12621266.
Parodi, PW 1999. Conjugated linoleic acid and other anticarcinogenic agents of bovine milk fat. Journal of Dairy Science 82, 13391349.
Petit, HV, Dewhurst, RJ, Scollan, ND, Proulx, JG, Khalid, M, Haresign, W, Twagiramungu, H, Mann, GE 2002. Milk production and composition, ovarian function, and prostaglandin secretion of dairy cows fed omega-3 fats. Journal of Dairy Science 85, 889899.
Petit, HV, Ivan, M, Mir, PS 2005. Effects of flaxseed on protein requirements and N excretion of dairy cows fed diets with two protein concentrations. Journal of Dairy Science 88, 17551764.
Piperova, LS, Sampugna, J, Teter, BB, Kalscheur, KF, Yurawecz, MP, Ku, Y, Morehouse, KM, Erdman, RA 2002. Duodenal and milk trans octadecenoic acid and conjugated linoleic acid (CLA) isomers indicate that post-absorptive synthesis is the predominant source of cis-9-containing CLA in lactating dairy cows. Journal of Nutrition 132, 12351241.
Precht, DMolkentin, J 1999. 18:1, 18:2 and 18:3 trans and cis fatty acid isomers including conjugated cisΔ9, transΔ11 linoleic acid (CLA) as well as total fat composition of german human milk lipids. Nahrung 43, 233244.
Schingoethe, DJ, Brouk, MJ, Lightfield, KD, Baer, RJ 1996. Lactational responses of dairy cows fed unsaturated fat from extruded soybeans or sunflower seeds. Journal of Dairy Science 79, 12441249.
Simopoulos, AP 2002. The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomedical Pharmacotherapy 56, 365379.
Statistical Packages for the Social Sciences 1998. SYSTAT version 9. SPPS Inc., Chicago, IL.
Troegeler-Meynadier, A, Nicot, MC, Bayourthe, C, Moncoulon, R, Enjalbert, F 2003. Effects of pH and concentration of linoleic and linolenic acids on extent and intermediates of ruminal biohydrogenation in vitro. Journal of Dairy Science 86, 40544063.
Van Soest, PJ, Robertson, JB, Lewis, BA 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 35833597.
Ward, PFV, Scott, TW, Dawson, RMC 1964. The hydrogenation of unsaturated fatty acid in the ovine digestive tract. Biochemical Journal 92, 6068.
Wolff, RL, Precht, D, Molkentin, J 1998. Occurrence and distribution profiles of trans-18:1 acids in edible fats of natural origin. In Trans fatty acids in human nutrition (ed. JL Sébédio and WW Christie), pp. 133. The Oily Press Ltd, Bridgewater, UK.
Yurawecz, MP, Hood, JK, Roach, JAG, Mossoba, MM, Daniels, DH, Ku, Y, Pariza, MW, Chin, SF 1994. Conversion of allylic hydroxy oleate to conjugated linoleic acid and methoxy oleate by acid-catalyzed methylation procedures. Journal of the American Oil Chemists Society 71, 11491155.

Keywords

Conjugated linolenic acid (CLnA), conjugated linoleic acid (CLA) and other biohydrogenation intermediates in plasma and milk fat of cows fed raw or extruded linseed

  • F. Akraim (a1) (a2), M. C. Nicot (a1), P. Juaneda (a3) and F. Enjalbert (a1)

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