Skip to main content Accessibility help
×
Home
Hostname: page-component-559fc8cf4f-6pznq Total loading time: 2.487 Render date: 2021-03-03T03:40:48.380Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false, "newCitedByModal": true }

Article contents

Relationship between efficiency of nitrogen utilization and isotopic nitrogen fractionation in dairy cows: contribution of digestion v. metabolism?

Published online by Cambridge University Press:  24 September 2015

G. Cantalapiedra-Hijar
Affiliation:
Institut National de la Recherche Agronomique, UMR 1213 INRA-VetAgro Sup, Unité Mixte de Recherches sur les Herbivores, 63122 St Genès Champanelle, France Clermont Université, VetAgro Sup, UMR 1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France
H. Fouillet
Affiliation:
Institut National de la Recherche Agronomique, CRNH-IdF, UMR 914 Unité Physiologie de la Nutrition et du Comportement Alimentaire, 75005 Paris, France AgroParisTech, CRNH-IdF, UMR 914 Unité Physiologie de la Nutrition et du Comportement Alimentaire, 75005 Paris, France
J. F. Huneau
Affiliation:
Institut National de la Recherche Agronomique, CRNH-IdF, UMR 914 Unité Physiologie de la Nutrition et du Comportement Alimentaire, 75005 Paris, France AgroParisTech, CRNH-IdF, UMR 914 Unité Physiologie de la Nutrition et du Comportement Alimentaire, 75005 Paris, France
A. Fanchone
Affiliation:
Institut National de la Recherche Agronomique, UR143 Zootechnie, 97170 Petit-Bourg, Guadeloupe, France
M. Doreau
Affiliation:
Institut National de la Recherche Agronomique, UMR 1213 INRA-VetAgro Sup, Unité Mixte de Recherches sur les Herbivores, 63122 St Genès Champanelle, France Clermont Université, VetAgro Sup, UMR 1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France
P. Nozière
Affiliation:
Institut National de la Recherche Agronomique, UMR 1213 INRA-VetAgro Sup, Unité Mixte de Recherches sur les Herbivores, 63122 St Genès Champanelle, France Clermont Université, VetAgro Sup, UMR 1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France
I. Ortigues-Marty
Affiliation:
Institut National de la Recherche Agronomique, UMR 1213 INRA-VetAgro Sup, Unité Mixte de Recherches sur les Herbivores, 63122 St Genès Champanelle, France Clermont Université, VetAgro Sup, UMR 1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France
Corresponding
Get access

Abstract

Animal tissues are naturally 15N enriched relative to their diet and the extent of this difference (Δ15Nanimal-diet) has been correlated to the efficiency of N assimilation in different species. The rationale is that transamination and deamination enzymes, involved in amino acid metabolism are likely to preferentially convert amino groups containing 14N over 15N. However, in ruminants the contribution of rumen bacterial metabolism relative to animal tissues metabolism to naturally enrich animal proteins in terms of 15N has been not assessed yet. The objective of this study was to assess the impact of rumen and digestion processes on the relationship between Δ15Nanimal-diet and efficiency of N utilization for milk protein yield (milk N efficiency (MNE); milk N yield/N intake) as well as the relationship between the 15N natural abundance of rumen bacteria and the efficiency of N use at the rumen level. Solid- and liquid-associated rumen bacteria, duodenal digesta, feces and plasma proteins were obtained (n=16) from four lactating Holstein cows fed four different diets formulated at two metabolizable protein supplies (80% v. 110% of protein requirements) crossed by two different dietary energy source (diets rich in starch v. fiber). We measured the isotopic N fractionation between animal and diet (Δ15Nanimal-diet) in these different body pools. The Δ15Nanimal-diet was negatively correlated with MNE when measured in solid-associated rumen bacteria, duodenal digesta, feces and plasma proteins, with the strongest correlation found for the latter. However, our results showed a very weak 15N enrichment of duodenal digesta (Δ15Nduodenal digesta-diet mean value=0.42) compared with that observed in plasma proteins (Δ15Nplasma protein-diet mean value=2.41). These data support the idea that most of the isotopic N fractionation observed in ruminant proteins (Δ15Nplasma protein-diet) has a metabolic origin with very little direct impact of the overall digestion process on the existing relationship between Δ15Nplasma protein-diet and MNE. The 15N natural abundance of rumen bacteria was not related to either rumen N efficiency (microbial N/available N) or digestive N efficiency (metabolizable protein supply/CP intake), but showing a modest positive correlation with rumen ammonia concentration. When using diets not exceeding recommended protein levels, the contribution of rumen bacteria and digestion to the isotopic N fractionation between animal proteins and diet is low. In our conditions, most of the isotopic N fractionation (Δ15Nplasma protein-diet) could have a metabolic origin, but more studies are warranted to confirm this point with different diets and approaches.

Type
Research Article
Information
Copyright
© The Animal Consortium 2015 

Access options

Get access to the full version of this content by using one of the access options below.

References

Al-Dehneh, A, Huber, JT, Wanderley, R, Theurer, CB, Pessarakli, M and DeYoung, D 1997. Incorporation of recycled urea—N into ruminal bacteria flowing to the small intestine of dairy cows fed a high-grain or high-forage diet. Animal Feed Science and Technology 68, 327338.CrossRefGoogle Scholar
Bach, A, Calsamiglia, S and Stern, MD 2005. Nitrogen metabolism in the rumen. Journal of Dairy Science 88, E9E21.CrossRefGoogle ScholarPubMed
Bauchart, D, Legay-Carmier, F, Doreau, M and Gaillard, B 1990. Lipid metabolism of liquid-associated and solid-adherent bacteria in rumen contents of dairy cows offered lipid-supplemented diets. British Journal of Nutrition 63, 563578.CrossRefGoogle ScholarPubMed
Bertram, HC, Yde, CC, Zhang, X and Kristensen, NB 2011. Effect of dietary nitrogen content on the urine metabolite profile of dairy cows assessed by nuclear magnetic resonance (NMR)-based metabolomics. Journal of Agricultural and Food Chemistry 56, 1249912505.CrossRefGoogle Scholar
Cabrita, ARJ, Fonseca, AJM and Dewhurst, RJ 2014. Short communication: relationship between the efficiency of utilization of feed nitrogen and 15N enrichment in casein from lactating dairy cows. Journal of Dairy Science 97, 72257229.CrossRefGoogle ScholarPubMed
Cantalapiedra-Hijar, G, Ortigues-Marty, I, Sepchat, B, Agabriel, J, Huneau, JF and Fouillet, H 2015. Diet–animal fractionation of nitrogen stable isotopes reflects the efficiency of nitrogen assimilation in ruminants. British Journal of Nutrition 113, 11581169.CrossRefGoogle ScholarPubMed
Castillo, AR, Kebreab, E, Beever, DE, Barbi, JH, Sutton, JD, Kirby, HC and France, J 2001. The effect of protein supplementation on nitrogen utilization in lactating dairy cows fed grass silage diets. Journal of Animal Science 79, 247253.CrossRefGoogle ScholarPubMed
Cheng, L, Kim, EJ, Merry, RJ and Dewhurst, RJ 2011. Nitrogen partitioning and isotopic fractionation in dairy cows consuming diets based on a range of contrasting forages. Journal of Dairy Science 94, 20312041.CrossRefGoogle ScholarPubMed
Cheng, L, Sheahan, AJ, Gibbs, SJ, Rius, AG, Kay, JK, Meier, S, Edwards, R and Roche, JR 2013. Technical note: nitrogen isotopic fractionation can be used to predict nitrogen-use efficiency in dairy cows fed temperate pasture. Journal of Animal Science 91, 57855788.CrossRefGoogle ScholarPubMed
Codron, D, Sponheimer, M, Codron, J, Hammer, S, Tschuor, A, Braun, U, Bernasconi, SM and Clauss, M 2012. Tracking the fate of digesta 13C and 15N compositions along the ruminant gastrointestinal tract: does digestion influence the relationship between diet and feces? European Journal of Wildlife Research 58, 303313.CrossRefGoogle Scholar
Cotta, MA and Russell, JB 1982. Effect of peptides and amino acids on efficiency of rumen bacterial protein synthesis in continuous culture. Journal of Dairy Science 65, 226234.CrossRefGoogle Scholar
DeNiro, MJ and Epstein, S 1981. Influence of diet on the distribution of nitrogen isotopes in animals. Geochimica et Cosmochimica Acta 45, 341351.CrossRefGoogle Scholar
Dijkstra, J, Reynolds, CK, Kebreab, E, Bannink, A, Ellis, JL, France, J and Van Vuuren, AM 2013. Challenges in ruminant nutrition: towards minimal nitrogen losses in cattle. In Energy and protein metabolism and nutrition in sustainable animal production (ed. Oltjen JW, Kebreab E and Lapierre H), pp. 4758. Wageningen Academic Publishers, The Netherlands.CrossRefGoogle Scholar
Fanchone, A, Nozière, P, Portelli, J, Duriot, B, Largeau, V and Doreau, M 2013. Effects of nitrogen underfeeding and energy source on nitrogen ruminal metabolism, digestion, and nitrogen partitioning in dairy cows. Journal of Animal Science 91, 895906.CrossRefGoogle ScholarPubMed
Gannes, LZ, Martinez del Rio, C and Koch, P 1998. Natural abundance variations in stable isotopes and their potential uses in animal physiological ecology. Comparative Biochemistry and Physiology 119, 725737.CrossRefGoogle ScholarPubMed
Gaye-Siessegger, J, Focken, U, Muetzel, S, Abel, H and Becker, K 2004. Feeding level and individual metabolic rate affect δ13C and δ15N values in carp: implications for food web studies. Oecologia 138, 175183.CrossRefGoogle Scholar
Godden, SM, Lissemore, KD, Kelton, DF, Leslie, KE, Walton, JS and Lumsden, JH 2001. Relationships between milk urea concentrations and nutritional management, production, and economic variables in Ontario dairy herds. Journal of Dairy Science 84, 11281139.CrossRefGoogle ScholarPubMed
Hof, G, Vervoorn, MD, Lenaers, PL and Tamminga, S 1997. Milk urea nitrogen as a tool to monitor the protein nutrition of dairy cows. Journal of Dairy Science 80, 33333340.CrossRefGoogle ScholarPubMed
Huhtanen, P, Cabezas-Garcia, EH, Krizsan, SJ and Shingfield, KJ 2015. Evaluation of between-cow variation in milk urea and rumen ammonia nitrogen concentrations and the association with nitrogen utilization and diet digestibility in lactating cows. Journal of Dairy Science 98, 31823196.CrossRefGoogle ScholarPubMed
INRA (ed.) 2007. Alimentation des bovins, ovins et caprins – Besoins des animaux – Valeurs des aliments – Tables INRA 2007. Editions Quae, Versailles, France.Google Scholar
Jonker, JS, Kohn, RA and Erdman, RA 1998. Using milk urea nitrogen to predict nitrogen excretion and utilization efficiency in lactating dairy cows. Journal of Dairy Science 81, 26812692.CrossRefGoogle ScholarPubMed
Kohn, RA, Kalscheur, KF and Russek-Cohen, E 2002. Evaluation of models to estimate urinary nitrogen and expected milk urea nitrogen. Journal of Dairy Science 85, 227233.CrossRefGoogle ScholarPubMed
Macko, SA and Estep, ML 1984. Microbial alteration of stable nitrogen and carbon isotopic compositions of organic matter. Organic Geochemistry 6, 787790.CrossRefGoogle Scholar
Macko, SA, Estep, MLF, Engel, MH and Hare, PE 1986. Kinetic fractionation of stable nitrogen isotopes during amino acid transamination. Geochimica et Cosmochimica Acta 50, 21432146.CrossRefGoogle Scholar
Macko, SA, Fogel, ML, Hare, PE and Hoering, TC 1987. Isotopic fractionation of nitrogen and carbon in the synthesis of amino acids by microorganisms. Chemical Geology: Isotope Geoscience Section 65, 7992.CrossRefGoogle Scholar
Martin, C, Williams, AG and Michalet-Doreau, B 1994. Isolation and characteristics of the protozoal and bacterial fractions from bovine ruminal contents. Journal of Animal Science 72, 29622968.CrossRefGoogle ScholarPubMed
Michalet-Doreau, B, Fernandez, I, Peyron, C, Millet, L and Fonty, G 2001. Fibrolytic activities and cellulolytic bacterial community structure in the solid and liquid phases of rumen contents. Reproduction Nutrition Development 41, 187194.CrossRefGoogle ScholarPubMed
Nolan, JV and Leng, RA 1972. Dynamic aspects of ammonia and urea metabolism in sheep. British Journal of Nutrition 27, 177194.CrossRefGoogle Scholar
Poupin, N, Mariotti, F, Huneau, JF, Hermier, D and Fouillet, H 2014. Natural isotopic signatures of variations in body nitrogen fluxes: a compartmental model analysis. PLOS Computational Biology 10, e1003865.CrossRefGoogle ScholarPubMed
Russell, JB, O’connor, JD, Fox, DG, Van Soest, PJ and Sniffen, CJ 1992. A net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation. Journal of Animal Science 70, 35513561.CrossRefGoogle ScholarPubMed
Sarraseca, A, Milne, E, Metcalf, MJ and Lobley, GE 1998. Urea recycling in sheep: effects of intake. British Journal of Nutrition 79, 7988.CrossRefGoogle Scholar
Sauvant, D and Nozière, P 2013. La quantification des principaux phénomènes digestifs chez les ruminants : les relations utilisées pour rénover les systèmes d'unités d'alimentation énergétique et protéique. Inra Productions Animales 26, 327346.Google Scholar
Sick, H, Roos, N, Saggau, E, Haas, K, Meyn, V, Walch, B and Trugo, N 1997. Amino acid utilization and isotope discrimination of amino nitrogen in nitrogen metabolism of rat liverin vivo. Zeitschrift für Ernährungswissenschaft 36, 340346.CrossRefGoogle ScholarPubMed
Sutoh, M, Koyama, T and Yoneyama, T 1987. Variations of natural 15N abundances in the tissues and digesta of domestic animals. Radioisotopes 36, 7477.CrossRefGoogle ScholarPubMed
Sutoh, M, Obara, Y and Yoneyama, T 1993. The effects of feeding regimen and dietary sucrose supplementation on natural abundance of 15N in some components of ruminal fluid and plasma of sheep. Journal of Animal Science 71, 226231.CrossRefGoogle ScholarPubMed
Tamminga, S 1992. Nutrition management of dairy cows as a contribution to pollution control. Journal of Dairy Science 75, 345357.CrossRefGoogle Scholar
Wang, P, Tan, Z, Guan, L, Tang, S, Zhou, C, Han, X, Kand, J and He, Z 2015. Ammonia and amino acids modulates enzymes associated with ammonia assimilation pathway by ruminal microbiota in vitro. Livestock Science 178, 130139.CrossRefGoogle Scholar
Wattiaux, MA and Reed, JD 1995. Fractionation of nitrogen isotopes by mixed ruminal bacteria. Journal of Animal Science 73, 257266.CrossRefGoogle ScholarPubMed
Weiss, PM, Chen, CY, Cleland, WW and Cook, PF 1988. Use of primary deuterium and N-15 isotope effects to deduce the relative rates of steps in the mechanisms of alanine and glutamate dehydrogenases. Biochemistry 27, 48144822.CrossRefGoogle Scholar
Wheadon, NM, McGee, M, Edwards, GR and Dewhurst, RJ 2014. Plasma nitrogen isotopic fractionation and feed efficiency in growing beef heifers. British Journal of Nutrition 111, 17051711.CrossRefGoogle ScholarPubMed

Altmetric attention score

Full text views

Full text views reflects PDF downloads, PDFs sent to Google Drive, Dropbox and Kindle and HTML full text views.

Total number of HTML views: 112
Total number of PDF views: 339 *
View data table for this chart

* Views captured on Cambridge Core between September 2016 - 3rd March 2021. This data will be updated every 24 hours.

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Relationship between efficiency of nitrogen utilization and isotopic nitrogen fractionation in dairy cows: contribution of digestion v. metabolism?
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Relationship between efficiency of nitrogen utilization and isotopic nitrogen fractionation in dairy cows: contribution of digestion v. metabolism?
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Relationship between efficiency of nitrogen utilization and isotopic nitrogen fractionation in dairy cows: contribution of digestion v. metabolism?
Available formats
×
×

Reply to: Submit a response


Your details


Conflicting interests

Do you have any conflicting interests? *