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Progesterone administration reduces the behavioural and physiological responses of ewes to abrupt weaning of lambs

Published online by Cambridge University Press:  19 April 2013

A. Freitas-de-Melo ,
G. Banchero ,
M. J. Hötzel ,
J. P. Damián  and
R. Ungerfeld
A. Freitas-de-Melo*
Affiliation:
Departamento de Biología Molecular y Celular, Universidad de la República, Lasplaces 1550, Montevideo 11600, Uruguay
G. Banchero
Affiliation:
Instituto Nacional de Investigación Agropecuaria, Ruta 50 km 12, La Estanzuela, Colonia 70000, Uruguay
M. J. Hötzel
Affiliation:
Laboratório de Etologia Aplicada, Departamento de Zootecnia e Desenvolvimento Rural, Universidade Federal de Santa Catarina, Rodovia Admar Gonzaga, 1346 Florianópolis, SC 88.034-001, Brazil
J. P. Damián
Affiliation:
Departamento de Biología Molecular y Celular, Universidad de la República, Lasplaces 1550, Montevideo 11600, Uruguay
R. Ungerfeld
Affiliation:
Departamento de Fisiología, Facultad de Veterinaria, Lasplaces 1550, Montevideo 11600, Uruguay
*
†E-mail: alinefreitasdemelo@hotmail.com
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Abstract

Abrupt weaning, a usual management in sheep productive systems, may provoke behavioural and physiological responses indicative of stress in ewes and lambs. Progesterone (P4) has anxiolytic and sedative effects through the union of its metabolites that contain 3α-hydroxyl group to the γ-aminobutyricacidA receptor. Our first aim was to determine whether P4 administration reduces the behavioural and physiological responses of ewes to abrupt weaning of lambs. A complementary aim was to determine whether P4 treatment affects the milk yield and composition of ewes, and the BW of their lambs. In experiment 1, seven ewes received P4 treatment for 32 days (group E1-P4), and eight ewes remained as an untreated control group (group E1-C). BW of the lambs was recorded during P4 treatment. Lambs were weaned at 59 days (Day 0 = weaning). The main behaviours of the ewes before and after weaning were recorded using 10 min scan sampling. The ewes’ serum total protein, albumin and globulin concentrations were measured before and after weaning of the lambs. In experiment 2, milk yield and composition were determined in two different groups of six ewes treated with P4 (group E2-P4) for 16 days and in five untreated controls (group E2-C). The BW of lambs increased with time (P = 0.001) in both groups and did not differ. The percentage of observations in which the ewes were seen pacing on Day 0 was greater in the E1-C group than in the E1-P4 group (P = 0.0007). Similarly, the percentage of observations in which the ewes were recorded vocalizing on Day 0 was greater in the E1-C group than in the E1-P4 group (P = 0.04). The percentage of observations in which E1-C ewes were recorded lying did not change from Days 0 to 1; however, it increased in E1-P4 ewes. Total serum protein concentration did not change in E1-P4 ewes from Days 0 to 3, although a decrease was seen in E1-C ewes (P = 0.04). Serum globulin concentration was greater in E1-P4 ewes on Day 3 than in E1-C ewes (P = 0.0008). In experiment 2, there were no differences between E2-P4 and E2-C ewes in terms of milk yield, protein, fat and lactose content. Progesterone administration reduced the behavioural and physiological responses of ewes to abrupt weaning of lambs, and this effect was not mediated by changes in milk yield and composition, or by lambs’ BW.

Keywords

sheepprogesteronestresspacingmilk yield and composition
Type
Behaviour, welfare and health
Information
animal , Volume 7 , Issue 8 , August 2013 , pp. 1367 - 1373
Copyright
Copyright © The Animal Consortium 2013 

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References

Allison, RW 2012. Laboratory evaluation of plasma and serum proteins. In Veterinary hematology and clinical chemistry (ed. MA Thrall), 2nd edition, pp. 460–475. Blackwell Publishing, Oxford, UK.Google Scholar
Apple, JK, Minton, JE, Parsons, KM, Unruh, JA 1993. Influence of repeated restraint and isolation stress and electrolyte administration on pituitary-adrenal secretions, electrolytes, and other blood constituents of sheep. Journal of Animal Science 71, 71–77.CrossRefGoogle ScholarPubMed
Arnold, GW, Wallace, SR, Maller, RA 1979. Some factors involved in natural weaning processes in sheep. Applied Animal Ethology 5, 43–50.CrossRefGoogle Scholar
Barbaccia, ML, Serra, M, Purdy, RH, Biggio, G 2001. Stress and neuroactive steroids. International Review of Neurobiology 46, 243–272.Google Scholar
Bartlewski, PM, Beard, AP, Rawlings, NC 1999. An ultrasonographic study of luteal function in breeds of sheep with different ovulation rates. Theriogenology 52, 115–130.Google Scholar
Bitran, D, Shiekh, M, McLeod, M 1995. Anxiolytic effect of progesterone is mediated by the neurosteroid allopregnanolone at brain GABAA receptors. Journal of Neuroendocrinology 3, 171–177.Google Scholar
Brunton, PJ, McKay, AJ, Ochedalski, T, Piastowska, A, Rebas, E, Lachowicz, A, Russell, JA 2009. Central opioid inhibition of neuroendocrine stress responses in pregnancy in the rat is induced by the neurosteroid allopregnanolone. The Journal of Neuroscience 29, 6449–6460.Google Scholar
Cockram, MS, Imlah, P, Goddard, PJ, Harkiss, GD, Waran, NK 1993. The behavioural, endocrine and leucocyte response of ewes to repeated removal of lambs before the age of natural weaning. Applied Animal Behaviour Science 38, 127–142.Google Scholar
Crossley, KJ, Walker, DW, Beart, PM, Hirst, JJ 2000. Characterization of GABAA receptors in fetal, neonatal and adult ovine brain: region and age related changes and the effects of allopregnanolone. Neuropharmacology 39, 1514–1522.Google Scholar
Doney, JM, Peart, JN, Smith, WF, Louda, F 1979. A consideration of the technique for estimation of milk yield by suckled sheep and a comparison of estimates obtained by two methods in relation to the effect of breed, level of production and stage of lactation. Journal of Agricultural Sciences 92, 123–132.Google Scholar
Flower, F, Weary, D 2001. Effects of early separation on the dairy cow and calf: 2. Separation at 1 day and 2 weeks after birth. Applied Animal Behaviour Science 70, 275–284.Google Scholar
Hearly, PJ, Falk, RH 1974. Values of some biochemical constituents in the serum of clinically normal sheep. Australian Veterinary Journal 50, 302–305.Google Scholar
Lambert, JJ, Cooper, MA, Simmons, RDJ, Weir, CJ, Belelli, D 2009. Neurosteroids: endogenous allosteric modulators of GABAA receptors. Psychoneuroendocrinology 34, 48–58.Google Scholar
Lehner, PN 1996. Handbook of ethological methods, 2nd edition. Cambridge University Press, Cambridge, UK.Google Scholar
Manalu, W, Sumaryadi, MY, Sudjatmogo Satyaningtijas, AS 2000. Effect of superovulation prior to mating on milk production performance during lactation in ewes. Journal of Dairy Science 83, 477–483.Google Scholar
McKusick, BC, Wiltbank, MC, Sartori, R, Marnet, PG, Thomas, DL 2002. Effect of presence or absence of corpora lutea on milk production in East Friesian dairy ewes. Journal of Dairy Science 85, 790–796.Google Scholar
Napolitano, F, Rosa, D, Sevi, A 2008. Welfare implications of artificial rearing and early weaning in sheep. Applied Animal Behaviour Science 110, 58–72.Google Scholar
Napolitano, F, Marino, V, De Rosa, G, Capparelli, R, Bordi, A 1995. Influence of artificial rearing on behavioral and immune response of lambs. Applied Animal Behaviour Science 45, 245–253.Google Scholar
Orgeur, P, Bernard, S, Naciri, M, Nowak, R, Schaal, B, Levy, F 1999. Psycological consequences of two different weaning methods in sheep. Reproduction Nutrition Development 39, 231–244.Google Scholar
Orgeur, P, Mavric, N, Yvore, P, Bernard, S, Nowak, R, Schaal, B, Levy, F 1998. Artificial weaning in sheep: consequences on behavioural, hormonal and immuno-pathological indicators of welfare. Applied Animal Behaviour Science 58, 87–103.CrossRefGoogle Scholar
Patchev, VK, Hassan, AH, Holsboer, DF, Almeida, OF 1996. The neurosteroid tetrahydroprogesterone attenuates the endocrine response to stress and exerts glucocorticoid-like effects on vasopressin gene transcription in the rat hypothalamus. Neuropsychopharmacology 5, 533–540.Google Scholar
Pérez-León, I, Orihuela, A, Lidfors, L, Aguirre, V 2006. Reducing mother young separation distress by inducing ewes into oestrous into day of weaning. Animal Welfare 15, 383–389.Google Scholar
Poindron, P, Levy, F, Keller, M 2006. Maternal responsiveness and maternal selectivity in domestic sheep and goats: the two facets of maternal attachment. Developmental Psychobiology 49, 54–70.Google Scholar
Russel, AJF, Doney, JM, Gunn, RG 1969. Subjective assessment of body fat in live sheep. Journal of Agricultural Science 72, 451–454.CrossRefGoogle Scholar
Säkkinen, H, Tverdal, A, Eloranta, E, Dahl, E, Holand, Ø, Saarela, S, Ropstad, E 2005. Variation of plasma protein parameters in four free-ranging reindeer herds and in captive reindeer under defined feeding conditions. Comparative Biochemistry and Physiology 142, 503–511.Google Scholar
Smith, LW, Inskeep, EK 1970. Effect of progestins on lactation in the ewe. Journal of Animal Science 30, 957–959.Google Scholar
Sphor, L, Banchero, G, Correac, G, Osórioa, MTM, Quintans, G 2011. Early prepartum shearing increases milk production of wool sheep and the weight of the lambs at birth and weaning. Small Ruminant Research 99, 44–47.Google Scholar
Ungerfeld, R, Hötzel, MJ, Scarsi, A, Quintans, G 2011. Behavioral and physiological changes in early-weaned multiparous and primiparous beef cows. Animal 5, 1270–1275.Google Scholar
Weary, DM, Fraser, D 1995. Signalling need: costly signals and animal welfare assessment. Applied Animal Behaviour Science 44, 159–169.Google Scholar
Weary, DM, Chua, B 2000. Effects of early separation on the dairy cow and calf: 1. Separation at 6 h, 1 day and 4 days after birth. Applied Animal Behaviour Science 69, 177–188.Google Scholar
Weary, DM, Jasper, J, Hötzel, MJ 2008. Understanding weaning distress. Applied Animal Behaviour Science 110, 24–41.Google Scholar