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Effects of soybean isoflavones on the growth performance, intestinal morphology and antioxidative properties in pigs

Published online by Cambridge University Press:  05 June 2020

Y. P. Li
Affiliation:
Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing100081, China State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing100193, China
X. R. Jiang
Affiliation:
Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing100081, China
Z. X. Wei
Affiliation:
Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing100081, China
L. Cai
Affiliation:
Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing100081, China
J. D. Yin
Affiliation:
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing100193, China
X. L. Li*
Affiliation:
Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing100081, China
*
E-mail: lixilong@caas.cn
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Abstract

Soybean meal is rich in soybean isoflavones, which exhibit antioxidant, anti-inflammatory, antiviral and anticancer functions in humans and animals. This study was conducted to investigate the effects of soybean isoflavones on the growth performance, intestinal morphology and antioxidative properties in pigs. A total of 72 weaned piglets (7.45 ± 0.13 kg; 36 males and 36 females) were allocated into three treatments and fed corn-soybean meal (C-SBM), corn-soy protein concentrate (C-SPC) or C-SPC supplemented with equal levels of the isoflavones found in the C-SBM diet (C-SPC + ISF) for a 72-day trial. Each treatment had six replicates and four piglets per replicate, half male and half female. On day 42, one male pig from each replicate was selected and euthanized to collect intestinal samples. The results showed that compared to pigs fed the C-SPC diet, pigs fed the C-SBM and C-SPC + ISF diets had higher BW on day 72 (P < 0.05); pigs fed the C-SBM diet had significantly higher average daily gain (ADG) during days 14 to 28 (P < 0.05), with C-SPC + ISF being intermediate; pigs fed the C-SBM diet tended to have higher ADG during days 42 to 72 (P = 0.063), while pigs fed the C-SPC + ISF diet had significantly higher ADG during days 42 to 72 (P < 0.05). Moreover, compared to pigs fed the C-SPC diet, pigs fed the C-SBM diet tended to have greater villus height (P = 0.092), while pigs fed the C-SPC + ISF diet had significantly greater villus height (P < 0.05); pigs fed the C-SBM and C-SPC + ISF diets had significantly increased villus height-to-crypt depth ratio (P < 0.05). Compared with the C-SPC diet, dietary C-SPC + ISF tended to increase plasma superoxide dismutase activity on days 28 (P = 0.085) and 42 (P = 0.075) and reduce plasma malondialdehyde (MDA) content on day 42 (P = 0.089), as well as significantly decreased jejunal mucosa MDA content on day 42 (P < 0.05). However, no significant difference in the expression of tight junction genes among the three groups was found (P > 0.05). In conclusion, our results suggest that a long-term exposure to soybean isoflavones enhances the growth performance, protects the intestinal morphology and improves the antioxidative properties in pigs.

Type
Research Article
Information
animal , Volume 14 , Issue 11 , November 2020 , pp. 2262 - 2270
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of The Animal Consortium

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References

Akula, SM, Hurley, DJ, Wixon, RL, Wang, C and Chase, CC 2002. Effect of genistein on replication of bovine herpesvirus type 1. American Journal of Veterinary Research 63, 11241128.CrossRefGoogle ScholarPubMed
Andres, A, Donovan, SM and Kuhlenschmidt, MS 2009. Soy isoflavones and virus infections. The Journal of Nutritional Biochemistry 20, 563569.CrossRefGoogle ScholarPubMed
Banan, A, Zhang, LJ, Shaikh, M, Fields, JZ, Choudhary, S, Forsyth, CB, Farhadi, A and Keshavarzian, A 2005. θ isoform of protein kinase C alters barrier function in intestinal epithelium through modulation of distinct claudin isotypes: a novel mechanism for regulation of permeability. Journal of Pharmacology and Experimental Therapeutics 313, 962982.Google ScholarPubMed
Calvello, R, Aresta, A, Trapani, A, Zambonin, C, Cianciulli, A, Salvatore, R, Clodoveo, ML, Corbo, F, Franchini, C and Panaro, MA 2016. Bovine and soybean milk bioactive compounds: effects on inflammatory response of human intestinal Caco-2 cells. Food Chemistry 210, 276285.Google ScholarPubMed
Chwen, LT, Foo, HL, Thanh, NT and Choe, DW 2013. Growth performance, plasma fatty acids, villous height and crypt depth of preweaning piglets fed with medium chain triacylglycerol. Asian-Australasian Journal of Animal Sciences 26, 700704.CrossRefGoogle ScholarPubMed
Cools, S, Van den Broeck, W, Vanhaecke, L, Heyerick, A, Bossaert, P, Hostens, M and Opsomer, G 2014. Feeding soybean meal increases the blood level of isoflavones and reduces the steroidogenic capacity in bovine corpora lutea, without affecting peripheral progesterone concentrations. Animal Reproduction Science 144, 7989.CrossRefGoogle ScholarPubMed
Gao, Y, Wang, X and He, C 2016. An isoflavonoid-enriched extract from Pueraria lobata (kudzu) root protects human umbilical vein endothelial cells against oxidative stress induced apoptosis. Journal of Ethnopharmacology 193, 524530.CrossRefGoogle ScholarPubMed
Greiner, LL, Stahly, TS and Stabel, TJ 2001a. The effect of dietary soy daidzein on pig growth and viral replication during a viral challenge. Journal of Animal Science 79, 31133119.CrossRefGoogle ScholarPubMed
Greiner, LL, Stahly, TS and Stabel, TJ 2001b. The effect of dietary soy genistein on pig growth and viral replication during a viral challenge. Journal of Animal Science 79, 12721279.CrossRefGoogle ScholarPubMed
Groschwitz, KR and Hogan, SP 2009. Intestinal barrier function: molecular regulation and disease pathogenesis. Journal of Allergy and Clinical Immunology 124, 320.CrossRefGoogle ScholarPubMed
Hämäläinen, M, Nieminen, R, Vuorela, P, Heinonen, M and Moilanen, E 2007. Anti-inflammatory effects of flavonoids: genistein, kaempferol, quercetin, and daidzein inhibit STAT-1 and NF-κB activations, whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-κB activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages. Mediators of Inflammation 2007, 110.Google ScholarPubMed
He, CM, Deng, J, Hu, X, Zhou, SC, Wu, JT, Xiao, D, Darko, KO, Huang, YJ, Tao, T, Peng, M, Wang, ZR and Yang, XP 2019. Vitamin A inhibits the action of LPS on the intestinal epithelial barrier function and tight junction proteins. Food & Function 10, 12351242.CrossRefGoogle ScholarPubMed
Hu, CH, Xiao, K, Luan, ZS and Song, J 2013. Early weaning increases intestinal permeability, alters expression of cytokine and tight junction proteins, and activates mitogen-activated protein kinases in pigs. Journal of Animal Science 91, 10941101.CrossRefGoogle ScholarPubMed
Kuhn, G, Hennig, U, Kalbe, C, Rehfeldt, C, Ren, MQ, Moors, S and Degen, GH 2004. Growth performance, carcass characteristics and bioavailability of isoflavones in pigs fed soy bean based diets. Archives of Animal Nutrition 58, 265276.CrossRefGoogle ScholarPubMed
Liang, J, Tian, YX, Fu, LM, Wang, TH, Li, HJ, Wang, P, Han, RM, Zhang, JP and Skibsted, LH 2008. Daidzein as an antioxidant of lipid: effects of the microenvironment in relation to chemical structure. Journal of Agricultural and Food Chemistry 56, 1037610383.CrossRefGoogle ScholarPubMed
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25, 402408.CrossRefGoogle Scholar
Mazumder, MAR and Hongsprabhas, P 2016. Genistein as antioxidant and antibrowning agents in in vivo and in vitro: a review. Biomedicine & Pharmacotherapy 82, 379392.CrossRefGoogle Scholar
National Research Council (NRC) 2012. Nutrient requirements of swine, 11th revised edition. National Academic Press, Washington, DC, USA.Google Scholar
Omonijo, FA, Liu, SX, Hui, QR, Zhang, H, Lahaye, L, Bodin, JC, Gong, JS, Nyachoti, MT and Yang, CB 2019. Thymol improves barrier function and attenuates inflammatory responses in porcine intestinal epithelial cells during lipopolysaccharide (LPS)-induced inflammation. Journal of Agricultural and Food Chemistry 67, 615624.CrossRefGoogle ScholarPubMed
Payne, RL, Bidner, TD, Southern, LL and Geaghan, JP 2001. Effects of dietary soy isoflavones on growth, carcass traits, and meat quality in growing-finishing pigs. Journal of Animal Science 79, 12301239.CrossRefGoogle ScholarPubMed
Pluske, JR, Thompson, MJ, Atwood, CS, Bird, PH, Williams, LH and Hartmann, PE 1996. Maintenance of villus height and crypt depth, and enhancement of disaccharide digestion and monosaccharide absorption, in piglets fed on cows’ whole milk after weaning. British Journal of Nutrition 76, 409422.Google ScholarPubMed
Rochell, SJ, Alexander, LS, Rocha, GC, Van Alstine, WG, Boyd, RD, Pettigrew, JE and Dilger, RN 2015. Effects of dietary soybean meal concentration on growth and immune response of pigs infected with porcine reproductive and respiratory syndrome virus. Journal of Animal Science 93, 29872997.CrossRefGoogle ScholarPubMed
Sarkar, FH and Li, Y 2003. Soy isoflavones and cancer prevention. Cancer Investigation 21, 744757.CrossRefGoogle ScholarPubMed
Smith, BN and Dilger, RN 2018. Immunomodulatory potential of dietary soybean-derived isoflavones and saponins in pigs. Journal of Animal Science 96, 12881304.CrossRefGoogle ScholarPubMed
Walsh, KR, Haak, SJ, Fastinger, ND, Bohn, T, Tian, Q, Mahan, DC, Schwartz, SJ and Failla, ML 2009. Gastrointestinal absorption and metabolism of soy isoflavonoids in ileal-canulated swine. Molecular Nutrition & Food Research 53, 277286.CrossRefGoogle ScholarPubMed
Wang, M, Yang, C, Wang, QY, Li, JZ, Huang, PF, Li, YL, Ding, XQ, Yang, HS and Yin, YL2020. The relationship between villous height and growth performance, small intestinal mucosal enzymes activities and nutrient transporters expression in weaned piglets. Journal of Animal Physiology and Animal Nutrition 104, 606615.CrossRefGoogle Scholar
Wijeratne, SS and Cuppett, SL 2007. Soy isoflavones protect the intestine from lipid hydroperoxide mediated oxidative damage. Journal of Agricultural and Food Chemistry 55, 98119816.CrossRefGoogle ScholarPubMed
Xiao, M, Ye, J, Tang, X and Huang, Y 2011. Determination of soybean isoflavones in soybean meal and fermented soybean meal by micellar electrokinetic capillary chromatography (MECC). Food Chemistry 126, 14881492.CrossRefGoogle Scholar
Xiao, Y, Mao, XB, Yu, B, He, J, Yu, J, Zheng, P, Huang, ZQ and Chen, D 2015. Potential risk of isoflavones: toxicological study of daidzein supplementation in piglets. Journal of Agricultural and Food Chemistry 63, 42284235.CrossRefGoogle ScholarPubMed
Yu, YB and Li, YQ 2014. Enteric glial cells and their role in the intestinal epithelial barrier. World Journal of Gastroenterology 20, 1127311280.CrossRefGoogle ScholarPubMed
Zhang, B and Guo, Y 2009. Supplemental zinc reduced intestinal permeability by enhancing occludin and zonula occludens protein-1 (ZO-1) expression in weaning piglets. British Journal of Nutrition 102, 687693.CrossRefGoogle ScholarPubMed
Zhang, QQ, Chen, DW, Yu, B, Mao, XB, Huang, ZQ, Yu, J, Luo, JQ, Zheng, P, Luo, YH and He, J 2018. Effects of dietary daidzein supplementation on reproductive performance, serum hormones, and reproductive-related genes in rats. Nutrients 10, 766778.Google ScholarPubMed
Zhao, XH, Chen, ZD, Zhou, S, Song, XZ, Ouyang, KH, Pan, K, Xu, LJ, Liu, CJ and Qu, MR 2017. Effects of daidzein on performance, serum metabolites, nutrient digestibility, and fecal bacterial community in bull calves. Animal Feed Science and Technology 225, 8796.Google Scholar
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