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Effect of dietary inclusion of 1% or 3% of native chicory inulin on the large intestinal mucosa proteome of growing pigs

Published online by Cambridge University Press:  13 March 2020

A. Herosimczyk Open the ORCID record for A. Herosimczyk [Opens in a new window] ,
A. Lepczyński ,
M. Ożgo ,
A. Tuśnio ,
M. Taciak  and
M. Barszcz
A. Herosimczyk*
Affiliation:
Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Janickiego 29 Str., 71-270Szczecin, Poland
A. Lepczyński
Affiliation:
Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Janickiego 29 Str., 71-270Szczecin, Poland
M. Ożgo
Affiliation:
Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Janickiego 29 Str., 71-270Szczecin, Poland
A. Tuśnio
Affiliation:
Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Janickiego 29 Str., 71-270Szczecin, Poland Department of Animal Nutrition, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 Str., 05-110Jabłonna, Poland
M. Taciak
Affiliation:
Department of Animal Nutrition, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 Str., 05-110Jabłonna, Poland
M. Barszcz
Affiliation:
Department of Animal Nutrition, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 Str., 05-110Jabłonna, Poland
*
†E-mail: agnieszka.herosimczyk@zut.edu.pl
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Abstract

Native chicory inulin is one of the promising alternatives to replace antibiotic growth promoters in young animals. Several potential mechanisms of prebiotic action have been proposed, such as modification of the intestinal microbiota composition leading to improved epithelial integrity and gut mucosal immunity of the host. The current study was focused on inulin effect on the large intestinal proteome and its implications for gut barrier functions. Therefore, we used proteomic techniques to determine changes in the large intestinal mucosa proteome of growing pigs after 40-day supplementation with native chicory inulin. The experiment was performed on 24 piglets fed from the 10th day of life an unsupplemented cereal-based diet or inulin-enriched diets (1% or 3%) with an average degree of polymerisation ≥ 10. At the age of 50 days, animals were sacrificed and tissue samples were collected from the cecum, and proximal and distal colon. Feeding diets supplemented with both levels of native inulin increased cecal and colonic expression of molecular chaperones, protein foldases and antioxidant proteins, which are collectively responsible for maintaining mucosal cell integrity as well as protecting against endotoxins and reactive oxygen species. This may confirm the beneficial effect of inulin on the gut health in growing pigs.

Keywords

pigletscecumcolonproteinsprebiotics
Type
Research Article
Information
animal , Volume 14 , Issue 8 , August 2020 , pp. 1647 - 1658
Copyright
© The Animal Consortium 2020

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