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Bioavailability of methionine hydroxy analog-calcium salt relative to dl-methionine to support nitrogen retention and growth in starter pigs

Published online by Cambridge University Press:  27 April 2012

F. O. Opapeju ,
J. K. Htoo ,
C. Dapoza  and
C. M. Nyachoti
F. O. Opapeju
Affiliation:
Department of Animal Science, Faculty of Agricultural and Food Sciences, University of Manitoba, Winnipeg, MB, Canada R3T 2N2
J. K. Htoo
Affiliation:
Evonik Industries AG, 63457 Hanau, Germany
C. Dapoza
Affiliation:
Evonik Degussa International AG, 08006 Barcelona, Spain
C. M. Nyachoti*
Affiliation:
Department of Animal Science, Faculty of Agricultural and Food Sciences, University of Manitoba, Winnipeg, MB, Canada R3T 2N2
*
†E-mail: martin_nyachoti@umanitoba.ca
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Abstract

This study assessed the efficacy of a calcium salt of methionine hydroxy analog (MHA-Ca, 84%) relative to dl-methionine (dl-Met, 99%) in starter pigs. In Experiment 1 (Exp. 1, N balance trial), 42 individually housed barrows (Genesus, average initial BW 19 kg) were used in two blocks of 21 pigs each with six replicates per treatment. Within each block, pigs were randomly allotted to seven diets: a methionine (Met)-deficient basal diet or the basal diet with three added levels of dl-Met (0.02%, 0.04% and 0.06%) or MHA-Ca (0.024%, 0.048% and 0.071%) on an equimolar basis. After a 7-day adaptation period, feces and urine were collected quantitatively for 5 days. Urinary and total N outputs decreased linearly (P < 0.05) with dl-Met or MHA-Ca supplementation. Nitrogen retention, expressed as g/day and as % of intake increased linearly (P < 0.01) with dl-Met and MHA-Ca supplementations. The relative efficacy of MHA-Ca to dl-Met was estimated to be 71.2% on a product-to-product basis for N retention expressed as % of intake. In Exp. 2 (performance trial), 280, 21-day-old crossbred (Pietrain × (Landrace × Large White)) pigs (eight pigs per pen, seven pens per treatment), were allocated to five diets in a completely randomized block design after a 10-day adaptation period. The Met-deficient basal diet contained 16.5% CP and 0.21% Met. Other diets were basal diet supplemented with two graded levels of dl-Met (0.04% and 0.08%) or MHA-Ca (0.062% and 0.12%) on a product basis at a dl-Met to MHA-Ca ratio of 65 : 100 at the expense of maize. BW and feed disappearance were monitored weekly for 3 weeks to determine performance. Final BW, average daily gain and average daily feed intake increased (P < 0.05) and feed to gain ratio decreased (P < 0.05) with the addition of Met to the basal diet irrespective of the source. Overall, the N retention results of Exp. 1 showed that the average relative bioavailability of MHA-Ca to dl-Met to support N retention (% of N intake) was 71% on a product- to-product basis (85% on an equimolar basis). In Exp. 2, pig performance was not different when Met was supplemented in the Met-deficient diet at a dl-Met to MHA-Ca ratio of 65 : 100 on a product basis.

Keywords

biological efficacydl-methioninemethionine-hydroxy analog calcium saltN balancepigs
Type
Nutrition
Information
animal , Volume 6 , Issue 11 , November 2012 , pp. 1750 - 1756
Copyright
Copyright © The Animal Consortium 2012

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References

Baker, DH 1994. Utilization of precursors for l-amino acids. In Amino acids in farm animal nutrition (ed. JPF D'Mello), pp. 37–64. CAB International, Wallingford, UK.Google Scholar
Boebel, KP, Baker, DH 1982. Efficacy of the calcium salt and free acid forms of methionine hydroxy analog for chicks. Poultry Science 61, 1167–1175.Google Scholar
Brown, JA, Cline, TR 1974. Urea excretion in pig: an indicator of protein quality and amino acid requirements. Journal of Nutrition 104, 542–545.Google Scholar
Christensen, AC, Anderson, JO, Dobson, DC 1980. Factors affecting efficacy of methionine hydroxy analog for chicks fed amino acid diets. Poultry Science 59, 2480–2484.Google Scholar
Chung, TK, Baker, DH 1992. Utilization of methionine isomers and analogs by the pig. Canadian Journal of Animal Science 72, 185–188.Google Scholar
Commission Directive 1998. Establishing community methods for the determination of amino-acids, crude oils and fats, and olanquindox in feeding stuff and amending Directive 71/393/EEC, annex part A. Determination of amino acids. Official Journal of the European CommunitiesL257, 14–23.Google Scholar
Commission Directive 2000. Establishing community methods for the determination of vitamin A, vitamin E and tryptophan, annex part C. Determination of tryptophan. Official Journal of the European Communities L 174, 45–50.Google Scholar
Dilger, RN, Baker, DH 2008. Cyst(e)ine imbalance and its effect on methionine precursor utilization in chicks. Journal of Animal Science 86, 1832–1840.Google Scholar
Drew, MD, van Kessel, AG, Maenz, DD 2003. Absorption of methionine and 2-hydroxy-4-methylthiobutanoic acid in conventional and germ-free chickens. Poultry Science 82, 1149–1153.Google Scholar
Elwert, C, Fernandes, ED, Lemme, A 2008. Biological effectiveness of methionine hydroxy-analogue calcium salt in relation to dl-methionine in broiler chickens. Asian-Australasian Journal of Animal Sciences 21, 1506–1515.CrossRefGoogle Scholar
Feng, Z, Qiao, S, Ma, Y, Wang, X, Li, X, Thacker, PA 2006. Efficacy of methionine hydroxyl analog and dl-methionine as methionine sources for growing pigs. Journal of Animal and Veterinary Advances 5, 135–142.Google Scholar
Huyghebaert, G 1993. Comparison of dl-methionine and methionine hydroxy analog-free acid in broilers by using multi-exponential regression models. British Poultry Science 34, 351–359.Google Scholar
Kim, BG, Lindemann, MD, Rademacher, M, Brennan, JJ, Cromwell, GL 2006. Efficacy of dl-methionine hydroxy analog free acid and dl-methionine as methionine sources for pigs. Journal of Animal Science 84, 104–111.Google Scholar
Knight, CD, Atwell, CA, Wuelling, CW, Ivey, FJ, Dibner, JJ 1998. The relative effectiveness of 2-hydroxy-4-(methylthio) butanoic acid and dl-methionine in young swine. Journal of Animal Science 76, 781–787.Google Scholar
Lingens, G, Molnar, S 1996. Studies on metabolism of broilers by using 14C-labelled dl-methionine and dl-methionine hydroxyl analogue Ca-salt. Archives of Animal Nutrition 49, 113–124.Google Scholar
Littell, RC, Lewis, AJ, Henry, PR 1995. Statistical evaluation of bioavailability assays. In Bioavailability of nutrients for animals – amino acids, minerals, and vitamins (ed. CB Ammerman, DH Baker and AJ Lewis), pp. 5–33. Academic Press Inc., San Diego, USA.Google Scholar
Littell, RC, Henry, PR, Lewis, AJ, Ammerman, CB 1997. Estimation of relative bioavailability of nutrients using SAS procedures. Journal of Animal Science 75, 2672–2683.Google Scholar
Llames, CR, Fontaine, J 1994. Determination of amino acids in feeds: collaborative study. Journal of Association of Official Analytical Chemists International 77, 1362–1402.Google Scholar
Malik, G, Hoehler, D, Rademacher, M, Drew, MD, Van Kessel, AG 2009. Apparent absorption of methionine and 2-hydroxy-4-methylthiobutanoic acid from gastrointestinal tract of conventional and gnotobiotic pigs. Animal 3, 1378–1386.Google Scholar
National Research Council (NRC) 1998. Nutrient requirements of swine, 10th edition. NRC, Washington, DC, USA.Google Scholar
Otto, ER, Yokoyama, M, Ku, PK, Ames, NK, Trottier, NL 2003. Nitrogen balance and ileal amino acid digestibility in growing pigs fed diets reduced in protein concentration. Journal of Animal Science 81, 1743–1753.Google Scholar
Payne, RL, Lemme, A, Seko, H, Hashimoto, Y, Fujisaki, H, Koreleski, J, Swiatkiewicz, S, Szczurek, W, Rostagno, H 2006. Bioavailability of methionine hydroxy analogue-free acid relative to dl-methionine in broilers. Animal Science Journal 77, 427–439.Google Scholar
Rademacher, M, Sauer, WC, Jansman, AJM 2001. Standardized ileal digestibility of amino acids in pigs. Evonik Degussa, Germany.Google Scholar
Reifsnyder, DH, Young, CT, Jones, EE 1984. The use of low protein liquid diets to determine the methionine requirement and the efficacy of methionine hydroxy analogue for the three-week old pig. Journal of Nutrition 114, 1705–1715.Google Scholar
Roemer, A, Abel, HJ 1999. Effects of -methionine hydroxy analogue (MHA) or -methionine (-Met) on N-retention in broiler chickens and pigs. Animal Feed Science and Technology 81, 193–203.Google Scholar
Scott, HM, Kelly, M, Huston, RL 1966. l-methionine versus methionine hydroxy analogue in basal diets containing either isolated soybean protein or crystalline amino acids. Poultry Science 45, 1123 (Abstract).Google Scholar
Shoveller, AK, Moehn, S, Rademacher, M, Htoo, JK, Ball, RO 2010. Methionine-hydroxy analogue was found to be significantly less bioavailable compared to dl-methionine for protein deposition in growing pigs. Animal 4, 61–66.Google Scholar
VDLUFA 1997. Bestimmung von DL-2-Hydroxy-4-methyl-mercaptobuttersäure nach Hydrolyse (Gesamt MHAR), Methode 4.11.4 Methodenbuch Band III. Die chemische Untersuchung von Futtermitteln, 4th Supplement. VDLUFA-Verlag, Darmstadt, Germany.Google Scholar
Yi, GF, Gaines, AM, Ratliff, BW, Srichana, P, Allee, GL, Perryman, KR, Knight, CD 2006. Estimation of the true ileal digestible lysine and sulfur amino acid requirement and comparison of the bioefficacy of 2-hydroxy-4-(methylthio)butanoic acid and dl-methionine in eleven- to twenty-six-kilogram nursery pigs. Journal of Animal Science 84, 1709–1721.Google Scholar
Zimmermann, B, Mosenthin, R, Rademacher, M, Lynch, PB, Esteve-Garcia, E 2005. Comparative studies on the relative efficacy of dl-methionine and liquid methionine hydroxy analogue in growing pigs. Asian-Australasian Journal of Animal Sciences 18, 1003–1010.Google Scholar