An experimental investigation was carried out of the incompressible boundary layer flow along a flat plate, in the presence of an adverse pressure gradient that corresponds to a linear retardation of the free stream velocity. The turbulence level in the free-stream was 0·12% and transition occurred with the laminar boundary layer being close to separation. For three values of the Reynolds number (2·56, 3·11 and 4·09 million based on the reference length that is defined as the reciprocal of the nondimensional-velocity gradient) the laminar, transitional and turbulent regions were studied by single (normal) hot-wire surveys at several streamwise positions. This allows the fluctuations of the streamwise velocity component to be followed from the amplification of laminar instability waves, through breakdown in the intermittency region, and the subsequent development towards a more or less developed turbulence structure. The study reveals that within the transition region fluctuation levels are reached throughout a large part of the boundary layer that are significantly higher than those in fully developed turbulent flow, which is partly a direct consequence of the intermittent character of the flow. For the highest Reynolds number additional cross-wire surveys were carried out in the turbulent region to observe the development of the turbulent stresses following transition. The data are interpreted in terms of structural coefficients, eddy viscosity and mixing length. Also, these results indicate that the transition process can be associated with turbulence levels well in excess of those occurring in fully developed turbulence, and reveal the relaxation of the outer region turbulence structure.