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  • Print publication year: 2013
  • Online publication date: November 2013

Section 4 - ART: skills, techniques and present status

References

1.Health and safety executive guidance, UK.
2. K. H. Lochner, T. Ballweg and H.-H. Fahrenkrog. Untersuchungen zur Messgenauigkeit von Kolbenhubpipetten mit Luftpolster. Lab Med 20, Nr. 7/8 (1996): S. 430–40.
3.International Bureau of Weights and Measures The International System of Units (SI), 8th edn (2006): 114–15. (http://www.bipm.org/utils/common/pdf/si_brochure_8_en.pdf
4. A. H. Sathananthan, A. Trounson, L. Freemann and T. Brady. The effects of cooling human oocytes. Hum Reprod 3, no. 8 (1998): 968–77.
5. S. J. Pickering, P. R. Braude, M. H. Johnson, A. Cant and J. Currie. Transient cooling to room temperature can cause irreversible disruption of the meiotic spindle in the human oocyte. Fertil Steril 54, no.1 (1990): 102–8.
6. P. A. Almeida and V. N. Bolton. The effect of temperature fluctuations on the cytoskeletal organisation and chromosomal constitution of the human oocyte. Zygote 3, no. 4 (1995): 357–65.
7. S. B. Shivhare, I. Ladas, J. Kashir, C. Jones, T. Griffiths, K. Turner and K. Coward. Light microscopy in clinical embryology: lighting the way for infertility treatment. Microscopy and Analysis 25, no. 2 (2011): 9–13.
8. A. Berkovitz, F. Eltes, A. Ellenbogen, S. Peer, D. Feldberg and B. Bartoov. Does the presence of nuclear vacuoles in human sperm selected for ICSI affect pregnancy outcome? Hum Reprod. 21 (2006): 1787–90.
9. J. Auger. Assessing human sperm morphology: top models, underdogs or biometrics? Asian J Androl 12 (2010): 36–46.
10. J. B. Oliveira, C. G. Petersen, F. C. Massaro, R. L. Baruffi, A. L. Mauri, L. F. Silva, J. Ricci and J. G. Franco, Jr. Motile sperm organelle morphology examination (MSOME): intervariation study of normal sperm and sperm with large nuclear vacuoles. Reprod Biol Endocrinol 8 (2010): 56.
11. B. Balaban, K. Yakin, C. Alatas, O. Oktem, A. Isiklar and B. Urman. Clinical outcome of intracytoplasmic injection of spermatozoa morphologically selected under high magnification: a prospective randomized study. Reprod Biomed Online, 2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd, 2011.
12. M. Wilding, G. Coppola, L. di Matteo, A. Palagiano, E. Fusco and B. Dale. Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa. J Assist Reprod Genet 28, no. 3 (2011): 253–62.
13. L. Rienzi, F. Ubaldi, F. Martinez, M. Iacobelli, M. G. Minasi, S. Ferrero, J. Tesarik and E. Greco. Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI. Hum Reprod 18, no. 6 (2003): 1289–93.
14. Y. Shen, T. Stalf, C. Mehnert, L. De Santis, I. Cino, H. R. Tinneberg and U. Eichenlaub-Ritter. Light retardance by human oocyte spindle is positively related to pronuclear score after ICSI. Reprod Biomed Online 12, no. 6 (2006): 737–51.
15. C. Madaschi, T. C. de Souza Bonetti, D. P. de Almeida Ferreira Braga, F. F. Pasqualotto, A. Iaconelli, Jr. and E. Borges, Jr. Spindle imaging: a marker for embryo development and implantation. Fertil Steril 90 (2008): 194–8.
16. W. H. Wang, L. Meng, R. J. Hackett, R. Odenbourg and D. L. Keefe. Limited recovery of meiotic spindles in living human oocytes after cooling-rewarming observed using polarized light microscopy. Hum Reprod 16, no. 11 (2001): 2374–8.
17. M. Montag and H. van der Ven. Symposium: innovative techniques in human embryo viability assessment. Oocyte assessment and embryo viability prediction: birefringence imaging. Reprod Biomed Online 17, no. 4 (2008): 454–60.
18. Y. Shen, T. Stalf, C. Mehnert, U. Eichenlaub-Ritter and H. R. Tinneberg. High magnitude of light retardation by the zona pellucida is associated with conception cycles. Hum Reprod 20 (2005): 1596–606.
19. G. A. Rama Raju, G. J. Prakash, K. M. Krishna and K. Madan. Meiotic spindle and zona pellucida characteristics as predictors of embryonic development: a preliminary study using PolScope imaging. Reprod Biomed Online 14, no. 2 (2007): 166–74.
20. M. Montag, M. Koster, K. van der Ven and H. van der Ven. Gamete competence assessment by polarizing optics in assisted reproduction. Hum Reprod Update 17, no. 5 (Sep–Oct 2011): 654–66.
21. L. Gianaroli, M. C. Magli, G. Collodel, E. Moretti, A. P. Ferraretti and B. Baccetti. Sperm head’s birefringence: a new criterion for sperm selection. Fertil Steril 90 (2008): 104–12.
22. L. Gianaroli, M. C. Magli, A. P. Ferraretti, A. Crippa, M. Lappi, S. Capitani and B. Baccetti. Birefringence characteristics in sperm heads allow for the selection of reacted spermatozoa for intracytoplasmic sperm injection. Fertil Steril 93 (2010): 807–13.
23. M. Meseguer, J. Herrero, A. Tejera, M. J. de los Santos, L. Eschrich, N. Garrido and N. Ramsing. Embryos with too early first cleavage fail to implant; correlation between exact timing of first cleavage and successful implantation. Hum Reprod 25, no. 1 (2010): 58–61.

Further reading

Practical skills in biomolecular sciences. Rob Reed, David Holmes, Jonathan Weyers and Allan Jones. 3d edn. Pearson Benjamin Cummings, 2007.
Practical laboratory skills training guides. Measurement of Volume. Elizabeth Pritchard. Royal Society of Chemistry. 2003.
Practical laboratory skills training guides. Measurement of pH. Elizabeth Pritchard. Royal Society of Chemistry, 2003.
Presentation skills: the essential guide for students. Patsy McCarthy and Caroline Hatcher. Sage Publications, 2002.
Lab math. A handbook of measurements, calculations and other quantitative skills for use at the bench. Dany Spencer Adams. Cold Spring Harbor Laboratory Press, 2003.
Fundamentals of light microscopy and electronic imaging. Douglas B. Murphy. Wiley-Liss, Inc., 2001.
Textbook of assisted reproductive technologies. Laboratory and clinical perspectives. David K. Gardner, Ariel Weissman, Colin M. Howles and Zeev Shoham, 3d edn. Informa UK Ltd, 2009.
Nikon microscopyU: The source for microscopy education. http://www.microscopyu.com/.

References

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3. World Health Organisation. WHO laboratory manual for the examination and processing of human semen, 5th edn (Cambridge: Cambridge University Press, 2010).
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5. D. Mortimer. Practical laboratory andrology. (Oxford: Oxford University Press, 1994), 241–2.
6. M. Vujkovic, J. H. de Vries, G. R. Dohle, G. J. Bonsel, J. Lindemans, N. S. Macklon, P. J. van der Spek E. A. Steegers, and R. P. Steegers-Theunissen. Associations between dietary patterns and semen quality in men undergoing IVF/ICSI treatment. Hum Reprod 24, no. 6 (2009 Jun):1304–12.
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8. D. S. Gaur, M. Talekar and V. P. Pathak. Alcohol intake and cigarette smoking: impact of two major lifestyle factors on male fertility. Indian J Pathol Microbiol 53, no. 1 (2010 Jan–Mar):35–40.
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10. G. De Fleurian, J. Perrin, R. Ecochard, E. Dantony, A. Lanteaume, V. Achard, J. M. Grillo, M. R. Guichaoua, A. Botta and I. Sari-Minodier. Occupational exposures obtained by questionnaire in clinical practice and their association with semen quality. J Androl 30, no. 5 (2009 Sep–Oct):566–79.
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13. S. Elzanaty. Time-to-ejaculation and the quality of semen produced by masturbation at a clinic. Urology 71, no. 5 (2008 May):883–8.
14. D. Mortimer. Sperm recovery techniques to maximize fertilizing capacity. Reprod Fertil Dev 6, no. 1 (1994):25–31.
15. D. Mortimer and S. Mortimer. Laboratory investigation of the infertile male. In Textbook of in vitro fertilisation and assisted reproduction, 3d edn (London: Informa Healthcare, 2005).
16. M. P. Milligan, S. J. Harris and K. J. Dennis. The effect of temperature on the velocity of human spermatozoa as measured by time-lapse photography. Fertil Steril 30, no. 5 (1978 Nov):592–4.
17. R. R. Henkel and W. B. Schill. Sperm preparation for ART. Reprod Biol Endrocrinol 1 (2003):108.
18. P. K. Iqbal, A. J. Adeghe, Y. Hughes, J. S. Samra, M. S. Obhari and J. Cuthbert. Clinical characteristics of subfertile men with antisperm antibodies. Br J Obstet Gynaecol 96, no. 1 (1989 Jan):107–10.
19. F. Lombardo, L. Gandini, F. Dondero and A. Lenzi. Antisperm immunity in natural and assisted reproduction. Hum Reprod Update 7, no. 5 (2001 Sep–Oct):450–6.
20. F. M. Helmerhorst, M. J. Finken and J. J. Erwich. Antisperm antibodies: detection assays for antisperm antibodies: what do they test? Hum Reprod 14, no. 7 (1999 Jul):1669–71.
21. R. S. Jeyendran, H. H. Van der Ven, M. Perez-Pelaez, B. G. Crabo and L. J. Zaneveld. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 70, no. 1 (1984 Jan):219–28.
22. W. C. Ford. Regulation of sperm function by reactive oxygen species. Hum Reprod Update 10, no. 5 (2004 Sep–Oct):387–99.
23. R. J. Aitken and J. S. Clarkson. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J Reprod Fertil 81, no. 2 (1987 Nov):459–69.
24. A. Borini, N. Tarozzi, D. Bizzaro, M. A. Bonu, L. Fava, C. Flamigni and G. Coticchio. Sperm DNA fragmentation: paternal effect on early post-implantation embryo development in ART. Hum Reprod 21, no. 11 (2006 Nov):2876–81.
25. D. Mortimer and S. T. Mortimer. Methods of sperm preparation for assisted reproduction. Ann Acad Med Singapore 21, no. 4 (1992 Jul):517–24.
26. R. J. Aitken and J. S. Clarkson. Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques. J Androl 9, no. 6 (1988 Nov–Dec):367–76.
27. R. S. Jeyendran. Sperm collection and processing methods – A practical guide. (Cambridge: Cambridge University Press, 2003).

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Further reading

D. De Ziegler, B. Borghese and C. Chapron. Endometriosis and infertility: pathophysiology and management. Lancet (2010): 730–8.
The Management of Tubal Pregnancy: Royal College of Obstetricians and Gynaecologists. Green Top Guidelines, no. 21, May 2004, Reviewed 2010.
I. Manyonda. Uterine Fibroids. (Elsevier, 2008).
National Institute of Clinical Excellence (NICE): Fertility Guidelines: 25 February 2004.

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Further reading

A. G. Johnson and P. R. V. Johnson. Making sense of medical ethics – a hands on guide. (London: Hodder Arnold, 2007).