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Chapter 24 - Sperm Selection for ICSI by Morphology

from Section 4 - Insemination/ICSI

Published online by Cambridge University Press:  11 May 2017

Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Fertility Associates, New Zealand
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Principles of IVF Laboratory Practice
Optimizing Performance and Outcomes
, pp. 163 - 171
Publisher: Cambridge University Press
Print publication year: 2017

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References

Bartoov, B., Berkovitz, A. and Eltes, F. Selection of spermatozoa with normal nuclei to improve the pregnancy rate with intracytoplasmic sperm injection. N Engl J Med 2001; 345:1067–8.Google Scholar
Vanderzwalmen, P., Hiemer, A., Rubner, P., Bach, M., Neyer, A. et al. Blastocyst development after sperm selection at high magnification is associated with size and number of nuclear vacuoles. Reprod Biomed Online 2008; 17:617–27.CrossRefGoogle ScholarPubMed
Neyer, A., Zintz, M., Stecher, A., Bach, M., Wirleitner, B. et al. The impact of paternal factors on cleavage stage and blastocyst development analyzed by time-lapse imaging: a retrospective observational study J Assist Reprod Genet 2015; 32:1607–14.CrossRefGoogle ScholarPubMed
Boitrelle, F., Albert, M., Petit, J.-M., Ferfouri, F., Wainer, R. et al. Small human sperm vacuoles observed under high magnification are pocket-like nuclear concavities linked to chromatin condensation failure. Reprod Biomed Online 2013; 27:201–11.Google Scholar
Cassuto, N. G., Hazout, A., Hammoud, I., Balet, R., Bouret, D. et al. Correlation between DNA defect and sperm-head morphology. Reprod Biomed Online 2012; 24, 211–18.Google Scholar
Garolla, A., Sartini, B., Cosci, I., Pizzol, D., Ghezzi, M. et al. Molecular karyotyping of single sperm with nuclear vacuoles identifies more chromosomal abnormalities in patients with testiculopathy than fertile controls: implications for ICSI. Hum Reprod 2015; 30:2493–500.CrossRefGoogle ScholarPubMed
Cassuto, G., Montjean, D., Siffroi, J. P., Bouret, D., Marzouk, F. et al. Different levels of DNA methylation detected in human sperms after morphological selection using high magnification microscopy. Biomed Res Int 2016; article ID 6372171, 1–7.CrossRefGoogle Scholar
Vanderzwalmen, P., Bach, M., Gaspard, O., Lejeune, B., Neyer, A. et al. Motile-sperm organelle-morphology examination and intracytoplasmic morphologically selected sperm injection: clinical and technical aspects. In A Practical Guide to Selecting Gametes and Embryos, ed. Montag, M. (pp. 5980 ) (Boca Raton, FL: CRC Press, 2014).Google Scholar
Cassuto, G., Hazout, A., Bouret, D., Balet, R., Larue, L. et al. Low birth defects by deselecting abnormal spermatozoa before ICSI. Reprod Biomed Online 2014; 28:4753.CrossRefGoogle ScholarPubMed

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