Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-18T22:20:15.188Z Has data issue: false hasContentIssue false

Chapter 14 - Technical Considerations in Perinatal CNS Tissue Processing

from Tissue Selection

Published online by Cambridge University Press:  07 August 2021

Mirna Lechpammer ,
Marc Del Bigio  and
Rebecca Folkerth
By
Mirna Lechpammer
Mirna Lechpammer
Affiliation:
New York University School of Medicine
Marc Del Bigio
Affiliation:
University of Manitoba, Canada
Rebecca Folkerth
Affiliation:
New York University School of Medicine
Get access

Summary

Traditionally, autopsy comprises of gross and microscopic examination, which routinely involves tissue fixation, processing, and paraffin embedding. Perinatal autopsies present with a variety of challenges, not the least of which involves the removal and examination of the immature brain. Even if great care is taken during brain removal, one may be left with an amorphous, semifluid mass of softened tissue by the time the brain is ready for fixation and further processing [1]. Therefore, in Appendix 1, we summarize the preparation of recommended fixative, manual, and automated procedures for the processing of perinatal brain tissue, which in our experience help to preserve CNS morphology and allow further downstream tissue studies.

Type
Chapter
Information
Perinatal Neuropathology , pp. 62 - 64
Publisher: Cambridge University Press
Print publication year: 2021

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Prahlow, JA, Ross, KF, Salzberger, L, Lott, EG, Guileyardo, JM, Barnard, JJ. Immersion technique for brain removal in perinatal autopsies. J Forensic Sci 1998;43(5):1056–60.CrossRefGoogle ScholarPubMed
Ernst, LM, Sondheimer, N, Deardorff, MA, Bennett, MJ, Pawel, BR. The value of the metabolic autopsy in the pediatric hospital setting. J Pediatr 2006;148(6):779–83.Google Scholar
Yamamoto, T, Nishio, H. Metabolic autopsy and molecular autopsy in sudden unexpected death in infancy. In: Ishikawa, T. (eds.) Forensic Medicine and Human Cell Research: Current Human Cell Research and Applications. Springer: Singapore; 2019. pp. 83–103.Google Scholar
Olpin, SE. The metabolic investigation of sudden infant death. Ann Clin Biochem 2004;41(4):282–93.Google Scholar
Christodoulou, J, Wilcken, B. Perimortem laboratory investigation of genetic metabolic disorders. Semin Neonatol 2004;9(4):275–80.Google Scholar

Save book to Kindle

To save this book to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×