Book contents
- Perinatal Neuropathology
- Perinatal Neuropathology
- Copyright page
- Contents
- Preface
- Acknowledgments
- Abbreviations
- Section I Techniques and Practical Considerations
- Approach to Autopsy of the Perinatal Nervous System
- Ancillary Studies
- Prosection Techniques
- Tissue Selection
- Chapter 13 Selection of Brain Areas for Microscopic Examination
- Chapter 14 Technical Considerations in Perinatal CNS Tissue Processing
- Chapter 15 Staining Techniques
- Reporting
- Section 2 Human Nervous System Development
- Section 3 Stillbirth
- Section 4 Disruptions / Hypoxic-Ischemic Injury
- Section 5 Malformations
- Section 6 Perinatal Neurooncology
- Section 7 Spinal and Neuromuscular Disorders
- Section 8 Eye Disorders
- Section 9 Infections: In Utero Infections
- Section 10 Metabolic / Toxic Disorders: Storage Diseases
- Section 11 Forensic Neuropathology
- Appendix 1 Technical Considerations in Perinatal CNS
- Index
- References
Chapter 15 - Staining Techniques
from Tissue Selection
Published online by Cambridge University Press: 07 August 2021
- Perinatal Neuropathology
- Perinatal Neuropathology
- Copyright page
- Contents
- Preface
- Acknowledgments
- Abbreviations
- Section I Techniques and Practical Considerations
- Approach to Autopsy of the Perinatal Nervous System
- Ancillary Studies
- Prosection Techniques
- Tissue Selection
- Chapter 13 Selection of Brain Areas for Microscopic Examination
- Chapter 14 Technical Considerations in Perinatal CNS Tissue Processing
- Chapter 15 Staining Techniques
- Reporting
- Section 2 Human Nervous System Development
- Section 3 Stillbirth
- Section 4 Disruptions / Hypoxic-Ischemic Injury
- Section 5 Malformations
- Section 6 Perinatal Neurooncology
- Section 7 Spinal and Neuromuscular Disorders
- Section 8 Eye Disorders
- Section 9 Infections: In Utero Infections
- Section 10 Metabolic / Toxic Disorders: Storage Diseases
- Section 11 Forensic Neuropathology
- Appendix 1 Technical Considerations in Perinatal CNS
- Index
- References
Summary
Numerous histochemical and immunohistochemical approaches are useful for investigation of the perinatal nervous system. The exact chemistry and methodology are beyond the scope of this textbook. The reader is referred to textbooks on these subjects, at least one of which should be readily available to the pathologist (1, 2). Online databases are also useful (e.g., www.histosearch.com). Most pathology laboratories will log the methods as Standard Operating Procedures for quality control. Details of solution concentration and timing may differ depending on whether the stains are done manually or using an automated processor; they are therefore not specified here. Here we will enumerate the advantages of several stains. It will be necessary to optimize the conditions in each laboratory. In general, we are concerned with formalin-fixed paraffin-embedded tissues (FFPE), with a typical section thickness of 4–6 µm. Some of the stains can also be applied to unfixed frozen sections, many of which are commonly used in the evaluation of muscle biopsies (see the section “Congenital Muscle Disorders”).
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- Perinatal Neuropathology , pp. 65 - 70Publisher: Cambridge University PressPrint publication year: 2021