Book contents
- In-Vitro Fertilization
- In-Vitro Fertilization
- Copyright page
- Contents
- Preface
- Acknowledgments
- Chapter 1 Review of Cell and Molecular Biology
- Chapter 2 Endocrine Control of Reproduction
- Chapter 3 Gametes and Gametogenesis
- Chapter 4 Gamete Interaction
- Chapter 5 First Stages of Development
- Chapter 6 Implantation and Early Stages of Fetal Development
- Chapter 7 Stem Cell Biology
- Chapter 8 The Clinical In-Vitro Fertilization Laboratory
- Chapter 9 Quality Management in the IVF Laboratory
- Chapter 10 Sperm and ART
- Chapter 11 Oocyte Retrieval and Embryo Culture
- Chapter 12 Cryopreservation of Gametes and Embryos
- Chapter 13 Micromanipulation Techniques
- Chapter 14 Preimplantation Genetic Diagnosis
- Chapter 15 Epigenetics and Human Assisted Reproduction
- Index
- References
Chapter 11 - Oocyte Retrieval and Embryo Culture
Published online by Cambridge University Press: 24 December 2019
- In-Vitro Fertilization
- In-Vitro Fertilization
- Copyright page
- Contents
- Preface
- Acknowledgments
- Chapter 1 Review of Cell and Molecular Biology
- Chapter 2 Endocrine Control of Reproduction
- Chapter 3 Gametes and Gametogenesis
- Chapter 4 Gamete Interaction
- Chapter 5 First Stages of Development
- Chapter 6 Implantation and Early Stages of Fetal Development
- Chapter 7 Stem Cell Biology
- Chapter 8 The Clinical In-Vitro Fertilization Laboratory
- Chapter 9 Quality Management in the IVF Laboratory
- Chapter 10 Sperm and ART
- Chapter 11 Oocyte Retrieval and Embryo Culture
- Chapter 12 Cryopreservation of Gametes and Embryos
- Chapter 13 Micromanipulation Techniques
- Chapter 14 Preimplantation Genetic Diagnosis
- Chapter 15 Epigenetics and Human Assisted Reproduction
- Index
- References
Summary
Every individual treatment cycle involves a number of different stages and manipulations in the laboratory, and each case must be assessed and prepared for in advance; the afternoon prior to the procedure (the day after hCG administration) is a convenient time to make the preparations. The laboratory staff should ensure that all appropriate consent forms have been signed by both partners, including consent for special procedures and storage of cryopreserved embryos. Details of any previous assisted conception treatment should be studied, including response to stimulation, number and quality of oocytes, timing of insemination, fertilization rate, embryo quality and embryo transfer procedure, and judgments regarding whether any parameters at any stage could be altered or improved in the present cycle can be assessed. The risk of introducing any infection into the laboratory via gametes and samples must be absolutely minimized: screening tests such as human immunodeficiency virus (HIV 1 and 2: Anti-HIV 1, 2) and hepatitis B (HbsAg/Anti-HBc) and C (Anti-HCV-Ab) should be confirmed, as well as any other tests indicated by the patients’ history (e.g., HTLV-I antibody, RhD, malaria, Trypanosoma cruzi, Zika virus). If donor gametes are to be used, additional tests for the donor are required: chlamydia, cytomegalovirus and a validated testing algorithm to exclude the presence of active infection with Treponema pallidum for syphilis testing.
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- In-Vitro Fertilization , pp. 213 - 253Publisher: Cambridge University PressPrint publication year: 2020