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  • Cited by 2
  • Print publication year: 2006
  • Online publication date: August 2009

10 - Parentage assessment among captive giant pandas in China

Summary

INTRODUCTION

While many recent advances have been made in the breeding of giant pandas ex situ, historically this species has never reproduced well in captivity. Sexual incompatibility, health problems, low fecundity and a juvenile mortality rate in excess of 70% have contributed to low reproductive success (O'Brien & Knight, 1987; O'Brien et al., 1994; Peng et al., 2001a, b). Wild- and captive-born giant pandas, particularly those captured at a young age, traditionally had difficulty producing offspring in captivity upon becoming adults (Lu & Kemf, 2001). As a result, the ex-situ giant panda population has not been self-sustaining and, until recently, its growth has relied on introducing animals captured from nature. In some cases, this included individuals that appeared ill (rescues) or cubs that were believed to be neglected or abandoned by their mothers. Later field studies, however, revealed that females often leave cubs alone for four to eight hours while foraging, and in one documented case for 52 hours (Lu et al., 1994). Recently, China has placed a general moratorium on capturing wild giant pandas for captive breeding (Lu & Kemf, 2001), a move that forces the breeding community to develop a self-sustaining population.

The goal, however, is not only ensuring demographic self-sustainability but also the maintenance of genetic diversity. The deleterious effects of inbreeding are well recognised (O'Brien, 1994a; Frankham, 1995; Hedrick & Kalinowski, 2001; Frankham et al., 2002).

References
Brownstein, M. J., Carpten, J. D. and Smith, J. R. (1996). Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. BioTechniques, 20, 1004–10.
Bruford, M. W and Wayne, R. K. (1993). Microsatellites and their application to population genetic studies. Current Opinions in Genetics and Development, 3, 939–43.
Callen, D. F., Thompson, A. D., Shen, Y.et al. (1993). Incidence and origin of ‘null’ alleles in the (AC)n microsatellite markers. American Journal of Human Genetics, 52, 922–7.
Ding, B., Zhang, Y. and Ryder, O. A. (1997). DNA preparation, PCR amplification and sequence analysis from scent marks and feces in giant panda. In Proceedings of the International Symposium on the Protection of the Giant Panda, ed. Zhang, A. and He, G.. Chengdu: Sichuan Publishing House of Science and Technology, pp. 157–62.
Ding, B., Shi, P., Xiangyu, J. G.et al. (2000). Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda. Chinese Science Bulletin, 45, 1883–5.
Driscoll, C. A., Menotti-Raymond, M. and O'Brien, S. J. (2002). Genomic microsatellites as evolutionary chronometers: a test in wild cats. Genome Research, 12, 414–23.
Fang, S., Ding, Z., Feng, W.et al. (1997a). A preliminary study of the material resource of DNA in the DNA fingerprinting analysis of giant pandas. Journal of Sichuan University, 34, 98–102.
Fang, S., Feng, W. and Zhang, A. (1997b). The development of giant panda DNA fingerprinting probe F2ZGP96060801 and the comparative test analysis of 5 probes. Acta Theriologica Sinica, 17, 165–71.
Fang, S., Feng, W., Zhang, A.et al. (1997c). DNA fingerprinting analysis on the paternity determination of giant pandas. Acta Theriologica Sinica, 17, 92–9.
Frankham, R. (1995). Conservation genetics. Annual Review of Genetics 29, 305–27.
Frankham, R., Ballou, J. D. and Briscoe, D. A. (2002). Introduction to Conservation Genetics. Cambridge: Cambridge University Press.
Goldstein, D. B. and Schlotterer, C. (1999). Microsatellites: Evolution and Applications. Oxford: Oxford University Press.
Hedrick, P. W. and Kalinowski, S. (2001). Inbreeding depression in conservation biology. Annual Review of Ecology and Systematics, 31, 139–62.
Huang, Y., Wang, P. Y., Zhang, H. M. et al. (2001). Efficiency of artificial insemination in giant pandas at the Wolong Breeding Center. Proceedings: American Society of Andrology, Journal of Andrology, Suppl, 118 (abstract 079), 146.
Lu, Z. and Kemf, E. (2001). Giant Pandas in the Wild. Gland, Switzerland: WWF-World Wide Fund for Nature.
Lu, Z., Wenshi, P. and Harkness, J. (1994). Mother–cub relationships in giant pandas in the Qinling Mountains, China, with comments on rescuing abandoned cubs. Zoo Biology, 13, 567–8.
Lu, Z., Johnson, W. E., Menotti-Raymond, M.et al. (2001). Patterns of genetic diversity in remaining giant panda populations. Conservation Biology, 15, 1596–607.
Marshall, T. C., Slate, J., Kruuk, L. E. B. and Pemberton, J. M. (1998). Statistical confidence for likelihood-based paternity inference in natural populations. Molecular Ecology, 7, 639–55.
Menotti-Raymond, M., David, V. A., Lyons, L. A.et al. (1999). A genetic linkage map of microsatellites in the domestic cat (Felis catus). Genomics, 57, 9–23.
O'Brien, S. J. (1994a). Genetic and phylogenetic analysis of endangered species. Annual Review of Genetics, 28, 467–89.
O'Brien, S. J.(1994b). A role for molecular genetics in biological conservation. Proceedings of the National Academy of Sciences, 91, 5748–55.
O'Brien, S. J. and Knight, J. A. (1987). The future of the giant panda. Nature, 325, 758–9.
O'Brien, S. J., Goldman, D., Knight, J.et al. (1984). Giant panda paternity. Science, 223, 1127–8.
O'Brien, S. J., Pan, W. and Lu, Z. (1994). Pandas, people and policy. Nature, 369, 179–80.
Peng, J., Jiang, Z. J. and Jinchu, H. (2001a). Status and conservation of giant panda (Ailuropoda melanoleuca): a review. Folia Zoologica, 50, 81–8.
Peng, J., Jiang, Z., Liu, W.et al. (2001b). Growth and development of giant panda (Ailuropoda melanoleuca) cubs at Beijing Zoo. Journal of Zoology (London), 254, 261–6.
Queller, D. C. and Goodnight, K. F. (1989). Estimating relatedness using genetic markers. Evolution, 43, 258–75.
Roelke, M. E., Martenson, J. S. and O'Brien, S. J. (1993). The consequences of demographic reduction and genetic depletion in the endangered Florida panther. Current Biology, 3, 340–50.
Ryder, O. A., McLaren, A., Brenner, S., Zhang, Y.-P. and Benirschke, K. (2001). DNA banks for endangered animal species. Science, 288, 275.
Uphyrkina, O., Miquelle, D., Quigley, H., Driscoll, C. A. and O'Brien, S. J. (2002). Conservation genetics of the Far Eastern leopard (Panthera pardus orientalis). Journal of Heredity, 93, 303–11.
Vigilant, L., Hofreiter, M., Seidel, H. and Boesch, C. (2001). Paternity and relatedness in wild chimpanzee communities. Proceedings of the National Academy of Sciences, 98, 12890–5.
Wildt, D. E. (2000). Genome resource banking for wildlife research, management and conservation. Institute for Laboratory Animal Research Journal, 41, 228–34.
Xie, Z. and Gipps, J. (2001). Giant Panda International Studbook. Beijing: Chinese Association of Zoological Gardens.
Zhang, Y., Ryder, O. A., Zhao, Q.et al. (1994). Non-invasive giant panda paternity exclusion. Zoo Biology, 13, 569–73.