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  • Print publication year: 2006
  • Online publication date: August 2009

7 - Male reproductive biology in giant pandas in breeding programmes in China



The goal of the giant panda ex situ breeding programme is to produce healthy, genetically diverse and reproductively sound offspring. However, reproduction in this species has been poor, in part, due to lack of male libido or aggressive behaviours towards conspecific females. Although giant panda breeding facilities have made progress in producing more surviving young, only about 29% of captive male giant pandas have ever sired offspring (Lindburg et al., 1998), and most of these males were wild born. Of the 104 giant pandas in the ex situ population in China in 1996 (at the time of the first masterplanning meeting in China; Zheng et al., 1997; see also Chapter 2), there were 33 adult males of reproductive age (6–26 years old). Only five (15.2%) had ever mated naturally and sired young. This was the main reason for ‘male reproduction’ being a primary target of the Biomedical Survey conducted under the umbrella of the Conservation Breeding Specialist Group (CBSG) (see Chapter 2).

We had three goals, the first being to measure the presence or absence of any obvious physiological or anatomical abnormalities. The second was to learn more about species reproductive biology, specifically comparing males of different ages, successful versus unsuccessful breeders and wild-born versus captive born. Our approach also allowed a third opportunity: studies that would enhance our understanding on how better to use male gametes (sperm) to advance genetic management (see Chapter 21). In this case, our focus was on:

sperm morphology and acrosomal integrity;

testes development during the breeding season;


Amann, R. P. (1986). Reproductive physiology and endocrinology of the dog. In Current Therapy in Theriogenology II, ed. Morrow, D. A.. Philadelphia, PA: W. B. Saunders Co., pp. 532–8.
Andrews, J. C., Howard, J. G., Bavister, B. D. and Wildt, D. E. (1992). Sperm capacitation in the domestic cat (Felis catus) and leopard cat (Felis bengalensis) as studied with a salt-stored zona pellucida penetration assay. Molecular Reproduction and Development, 31, 200–7.
Barone, M. A., Roelke, M. E., Howard, J. G., Anderson, A. E. and Wildt, D. E. (1994). Reproductive characterization of male Florida panthers: comparative studies from Florida, Texas, Colorado, Chile and North American zoos. Journal of Mammology, 75, 150–62.
Beyer, W. H. (1987). CRC Standard Mathematical Tables, 28th Edn. Boca Raton, FLCRC Press, p. 131.
Boatman, D. E., Andrews, J. C. and Bavister, B. D. (1988). A quantitative assay for capacitation: evaluation of multiple sperm penetration through the zona pellucida of salt-stored hamster eggs. Gamete Research, 19, 19–29.
Chen, D., Ye, Z. and Zhang, Z. (1984). Ultrastructure of giant panda spermatozoa. Acta Zoologica Sinica, 30, 301–4.
Chen, D., Shi, Q., Zhao, al. (1989a). Studies of heterofertilization in vitro between giant panda and golden hamster. Acta Zoologica Sinica, 35, 276–380.
Chen, D., Song, X., Zhou, X. and Duan, C. (1989b). Study on in vitro sperm capacitation and egg penetration of giant panda. Science in China, 32, 435–41.
Chen, D., Song, X., Feng, al. (1992). Study on the thawing solutions of cryopreserved semen pellets in the giant panda. Chinese Journal of Zoology, 27–63.
Drobnis, E., Yudin, A., Cherr, G. and Katz, D. (1988). Hamster sperm penetration of the zona pellucida: kinematic analysis and mechanical implications. Developmental Biology, 130, 311–23.
Durrant, B. S., Olson, M. A., Amodeo, al. (2003). Vaginal cytology and vulvar swelling as indicators of impending estrus and ovulation in the giant panda (Ailuropoda melanoleuca). Zoo Biology, 22, 313–21.
Feng, W. and Zhang, A. (1988). Giant Panda Theriogenology and Assisted Reproduction. Chengdu: Publishing Company of Sichuan University, pp. 32–8.
Gao, G., Mazur, P. and Critser, J. (1997). Fundamental cryobiology of mammalian spermatozoa. In Reproductive Tissue Banking: Scientific Principles, ed. Karow, A. and Critser, J.. San Diego, CA: Academic Press, pp. 263–328.
Hammerstedt, R. H., Graham, J. K. and Nolan, J. P. (1990). Cryopreservation of mammalian sperm: what we ask them to survive. Journal of Andrology, 11, 73–88.
Howard, J. G. (1993). Semen collection and analysis in nondomestic carnivores. In Zoo and Wild Animal Medicine: Current Therapy III, ed. Fowler, M. E.. Philadelphia, PA: W. B. Saunders Co., pp. 390–9.
Howard, J. G. (1999). Assisted reproductive techniques in nondomestic carnivores. In Zoo and Wild Animal Medicine: Current Therapy IV, ed. Fowler, M. and Miller, E.. Philadelphia, PA: W. B. Saunders Co., pp. 449–57.
Howard, J. G., Bush, M. and Wildt, D. E. (1986). Semen collection, analysis and cryopreservation in nondomestic mammals. In Current Therapy in Theriogenology II, ed. Morrow, D.. Philadelphia, PA: W. B. Saunders Co., pp. 1047–53.
Huang, Y., Wang, P., Zhang, G. et al. (2002). Use of artificial insemination to enhance propagation of giant pandas at the Wolong Breeding Center. In Proceedings of the Second International Symposium on Assisted Reproductive Technology for the Conservation and Genetic Management of Wildlife, ed. N. Loskutoff, Omaha's Henry Doorly Zoo, Omaha, NE, pp. 172–9.
Ishikawa, A., Matsui, M., Tsuruga, al. (1998). Electroejaculation and semen characteristics of the captive Hokkaido brown bear (Ursus arctos yesoensis). Journal of Veterinary Medicine Research, 60, 965–8.
Lindburg, D. G., Huang, X. M. and Huang, S. Q. (1998). Reproductive performance of male giant panda in Chinese zoos. In Proceedings of the International Symposium on the Protection of the Giant Panda, Chengdu: Sichuan Publishing House of Science and Technology, pp. 65–71.
Liu, W. (1981). A note on the artificial insemination of the giant panda. Acta Zoologica Sinica, 12, 73–6.
Mahi, C. A. and Yanagimachi, R. (1975). Induction of nuclear decondensation of mammalian spermatozoain vitro. Journal of Reproduction and Fertility 44, 293–6.
Mansfield, K. G. and Land, E. D. (2002). Cryptorchidism in Florida panthers: prevalence, features and influence of genetic restoration. Journal of Wildlife Diseases, 38, 693–8.
Masui, M., Hiramatsu, H., Nose, al. (1989). Successful artificial insemination in the giant panda (Ailuropoda melanoleuca) at Ueno Zoo. Zoo Biology, 8, 17–26.
Mazur, P. (1974). Freezing of living cells: mechanisms and implications. American Journal of Physiology, 247, C125–C142.
Moore, H., Bush, M., Celma, al. (1984). Artificial insemination in the giant panda (Ailuropoda melanoleuca). Journal of Zoology (London), 203, 269–78.
Olson, M. A., Huang, Y., Li, al. (2003). Assessment of motility, acrosomal integrity and viability of giant panda (Ailuropoda melanoleuca) sperm following short-term storage at 4°C. Zoo Biology, 22, 529–44.
Platz, C., Wildt, D. E., Howard, J. G. and Bush, M. (1983). Electroejaculation and semen analysis and freezing in the giant panda (Ailuropoda melanoleuca). Journal of Reproduction and Fertility, 67, 9–12.
Pope, C., Zhang, Y. and Dresser, B. (1991). A simple staining method for evaluating acrosomal status of cat spermatozoa. Journal of Zoo and Wildlife Medicine, 22, 87–95.
Roelke, M. E., Martenson, J. S. and O'Brien, S. J. (1993). The consequences of demographic reduction and genetic depletion in the endangered Florida panther. Current Biology, 3, 340–50.
Spindler, R. E., Huang, Y., Howard, J. al. (2004). Acrosomal integrity and capacitation are not influenced by sperm cryopreservation in the giant panda. Reproduction, 127, 547–56.
Sun, Q., Liu, H., Li, al. (1996). The role of calcium and protein kinase C in the acrosome reaction of giant panda spermatozoa. Theriogenology, 46, 359–67.
Wildt, D. E. and Swanson, W. F. (1998). Reproduction in cats. In Encyclopedia of Reproduction, ed. Knobil, E. and Neil, J.. New York, NY: Academic Press, Inc., pp. 497–510.
Wildt, D. E., Rall, W. F., Critser, J. K., Monfort, S. L. and Seal, U. S. (1997). Genome resource banks: ‘living collections’ for biodiversity conservation. BioScience, 47, 689–98.
Yanagimachi, R. (1994). Mammalian fertilization. In The Physiology of Reproduction, ed. Knobil, E. and Neil, J.. New York, NY: Raven Press, pp. 189–317.
Yanagimachi, R., Lopata, A., Odom, C. al. (1979). Retention of biologic characteristics of zona pellucida in highly concentrated salt solution: the use of salt-stored eggs for assessing the fertilizing ability of spermatozoa. Fertility and Sterility, 31, 562–74.
Ye, Z. Y., He, G. X., Zhang, A. al. (1991). Studies on the artificial pollination method of giant panda. Journal of Sichuan University (Natural Science), 28, 50–3.
Zhang, A. J., Ye, Z. Y., He, G. al. (1991). Studies on conception effect of frozen semen in the giant panda. Journal of Sichuan University (Natural Science), 28, 54–9.
Zheng, S., Zhao, Q., Xie, Z., Wildt, D. E. and Seal, U. S. (1997). Giant Panda Captive Planning Management Workshop Report. Apple Valley, MN: Conservation Breeding Specialist Group.