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  • Print publication year: 2016
  • Online publication date: March 2016

10 - Tiny samples from tiny lemurs: methodological considerations for endoparasite analyses in mouse lemurs

from Part II - Methods for studying captive and wild cheirogaleids

Summary

Introduction

Studies of parasite burden and transmission in wild non-human primates are important for combating emerging infectious diseases in humans, understanding processes of zoonosis, and managing and conserving endangered species (Gillespie et al., 2008). In a biodiversity hotspot facing extreme anthropogenic pressure, lemurs are a high conservation priority (Schwitzer et al., 2014) and the relationship between lemur health and human health is only beginning to be understood (Bublitz et al., 2015; Zohdy et al., 2015). Mouse lemurs (Microcebus spp.) are well positioned to become a very useful model for endoparasite studies in lemurs. As a highly specious genus with 21 recognized species, they are widespread across the island of Madagascar, occurring in diverse habitats including rainforests, dry deciduous forests, littoral forests, etc. (Radespiel, 2006; Sommer et al., 2014; Zimmermann and Radespiel, 2014). In addition, species vary (within and between species) in their diets, use of torpor, degree of sociality, and sympatry with congeneric species (Lahann et al., 2006; Radespiel, 2006; Sommer et al., 2014; Chapter 22). This variation, combined with the ease with which they can be trapped, examined, and sampled without anesthesia, thus yielding reasonable sample sizes, makes them attractive for asking a multitude of questions with implications for understanding host–parasite coevolution, transmission dynamics and zoonosis, conservation, and wildlife management.

Mouse lemurs harbor, in particular, vector-borne parasites and direct lifecycle parasites (Radespiel et al., 2015). They may become infected with vector-borne parasites when feeding on arthropods serving as intermediate hosts (Radespiel et al., 2015). In addition, they are vulnerable to parasites with direct lifecycles when feeding in contaminated vegetation or co-sleeping in tree holes/nests with infected individuals who may defecate in the sleeping sites (Sommer et al., 2014; Radespiel et al., 2015). The endoparasite studies conducted to date have included foci on relationships between parasites and seasonality (Raharivololona and Ganzhorn, 2010), forest fragmentation (Schad et al., 2005), the genetics of parasite resistance (Schad et al., 2005; Schwensow et al., 2010a, 2010b; Sommer et al., 2014), sympatry among host mouse lemur species (Sommer et al., 2014; Radespiel et al., 2015), and parasites with the potential to be zoonotic with human populations (Rasambainarivo et al., 2013; Bublitz et al., 2015; Zohdy et al., 2015). Many of these studies were conducted within the last five years and meeting abstracts suggest that several other studies are underway (e.g., Alldredge et al., 2013, 2014; Rodriguez et al., 2013).

Alldredge, KL, Irwin, MT, Godfrey, LR. 2013. Roundworms on the Red Island: gastrointestinal parasite intensity in four lemur species from the Tsinjoarivo region, Madagascar. American Journal of Physical Anthropology 150:66–66.
Alldredge, KL, Romer, ZC, Fitzpatrick, MC, Irwin, MT. 2014. Strongyloids in the soil: an exploration of gastrointestinal parasite load and geophagy in lemurs at Tsinjoarivo, Madagascar. American Journal of Physical Anthropology 153:64–65.
Bublitz, DC, Wright, PC, Rasambainarivo, FT, et al. 2015. Pathogenic enterobacteria in lemurs associated with anthropogenic disturbance. American Journal of Primatology 77(3):330–337.
Field, A. 2013. Discovering Statistics Using IBM SPSS Statistics. Sage, London.
Gillespie, TR. 2006. Noninvasive assessment of gastrointestinal parasite infections in free-ranging primates. International Journal of Primatology 27(4):1129–1143.
Gillespie, TR, Nunn, CL, Leendertz, FH. 2008. Integrative approaches to the study of primate infectious disease: implications for biodiversity conservation and global health. Yearbook of Physical Anthropology 51:53–69.
Jensen, B, Kepley, W, Guarner, J, et al. 2000. Comparison of polyvinyl alcohol fixative with three less hazardous fixatives for detection and identification of intestinal parasites. Journal of Clinical Microbiology 38:1592–1598.
Lahann, P, Schmid, J, Ganzhorn, JU. 2006. Geographic variation in populations of Microcebus murinus in Madagascar: resource seasonality or Bergmann's rule?International Journal of Primatology 27(4):983–999.
Radespiel, U. 2006. Ecological diversity and seasonal adaptations of mouse lemurs (Microcebus spp.). In Gould, L, Sauther, ML (eds.), Lemurs: Ecology and Adaptation (pp. 211–234). Springer, New York.
Radespiel, U, Schaber, K, Kessler, SE, Schaarschmidt, F, Strube, C. 2015. Variations in the excretion patterns of helminth eggs in two sympatric mouse lemur species (Microcebus murinus and M. ravelobensis) in northwestern Madagascar. Parasitology Research 114(3):941–954.
Raharivololona, BM, Ganzhorn, JU. 2010. Seasonal variations in gastrointestinal parasites excreted by the gray mouse lemur Microcebus murinus in Madagascar. Endangered Species Research 11(2):113–122.
Rasambainarivo, FT, Gillespie, TR, Wright, PC, et al. 2013. Survey of Giardia and Cryptosporidium in lemurs from the Ranomafana National Park, Madagascar. Journal of Wildlife Diseases 49(3):741–743.
Rodriguez, IA, Rasoazanabary, E, Godfrey, LR. 2013. What's eating Microcebus? Endo- and ectoparasite ecology of Microcebus griseorufus at Beza Mahafaly Special Reserve, Madagascar. American Journal of Physical Anthropology 150:234–234.
Schad, J, Ganzhorn, JU, Sommer, S. 2005. Parasite burden and constitution of major histocompatibility complex in the Malagasy mouse lemur, Microcebus murinus. Evolution 59(2):439–450.
Schwensow, N, Dausmann, K, Eberle, M, Fietz, J, Sommer, S. 2010a. Functional associations of similar MHC alleles and shared parasite species in two sympatric lemurs. Infection Genetics and Evolution 10(5):662–668.
Schwensow, N, Eberle, M, Sommer, S. 2010b. Are there ubiquitous parasite-driven major histocompatibility complex selection mechanisms in gray mouse lemurs?International Journal of Primatology 31(4):519–537.
Schwitzer, C, Mittermeier, RA, Johnson, SE, et al. 2014. Averting lemur extinctions amid Madagascar's political crisis. Science 343(6173):842–843.
Sommer, S, Rakotondranary, SJ, Ganzhorn, JU. 2014. Maintaining microendemic primate species along an environmental gradient – parasites as drivers for species differentiation. Ecology and Evolution 4(24):4751–4765.
Stuart, MD, Strier, KB. 1995. Primates and parasites – a case for a multidisciplinary approach. International Journal of Primatology 16(4):577–593.
Zimmermann, E, Radespiel, U. 2014. Species concepts, diversity, and evolution in primates: lessons to be learned from mouse lemurs. Evolutionary Anthropology 23:11–14.
Zohdy, S, Grossman, MK, Fried, IR, et al. 2015. Diversity and prevalence of diarrhea-associated viruses in the lemur community and associated human population of Ranomafana National Park, Madagascar. International Journal of Primatology 36(1):143–153.