Book contents
- Frontmatter
- Contents
- Contributors
- Preface
- Section one Overviews
- Chapter 1 Harnessing the Power of Chemistry for Biology and Medicine
- Chapter 2 Exploring Biology with Small Organic Molecules
- Chapter 3 Chemical Proteomics: A Global Study of Protein–Small Molecule Interactions
- Section two Molecules for Chemical Genomics
- Section Three Basics of High-Throughput Screening
- Section Four Chemical Genomics Assays and Screens
- Section five Chemical Genomics and Medicine
- Index
- References
Chapter 3 - Chemical Proteomics: A Global Study of Protein–Small Molecule Interactions
from Section one - Overviews
Published online by Cambridge University Press: 05 June 2012
- Frontmatter
- Contents
- Contributors
- Preface
- Section one Overviews
- Chapter 1 Harnessing the Power of Chemistry for Biology and Medicine
- Chapter 2 Exploring Biology with Small Organic Molecules
- Chapter 3 Chemical Proteomics: A Global Study of Protein–Small Molecule Interactions
- Section two Molecules for Chemical Genomics
- Section Three Basics of High-Throughput Screening
- Section Four Chemical Genomics Assays and Screens
- Section five Chemical Genomics and Medicine
- Index
- References
Summary
What is chemical proteomics?
Proteins exert their functions through interaction with other proteins or ligands. Therefore, to understand protein function, it is important to analyze protein-protein and protein-ligand interactions. As a result of the completion of the genome sequencing, studies of protein-protein interactions can be conducted on a genome-wide scale. Similarly, biologically active small molecules exert their actions through interaction with their targets, mostly proteins. Thus, the same holds true for analyses of chemicals and proteins. Chemical proteomics is an approach to clarify the biological function of proteins or chemicals through analyses of protein-chemical interactions.
As with genetics, there are two types of chemical proteomics, viz. forward chemical proteomics and reverse chemical proteomics [1]. Although the strategies are different, the primary goals of these two approaches are the same. Both methodologies employ chemicals of interest, so-called bioprobes, at the beginning [2]. The bioprobes with unique biological activities not only lead to drug discovery and development, but they also can be used for investigating many aspects of proteins, such as expression and subcellular localization. If the mode of action of a compound is unknown, we seek to identify the specific cellular targets in cells from the proteome. In forward chemical proteomics, whole-cell lysates are generally screened for interacting proteins, whereas in reverse chemical proteomics, purified or recombinant proteins expressed from the cloned ORFeome (a whole set of open reading frames in an organism) are employed for the target screen. These approaches have both advantages and disadvantages based on the differences in experimental procedures. This section describes their features and typical examples.
- Type
- Chapter
- Information
- Chemical Genomics , pp. 26 - 36Publisher: Cambridge University PressPrint publication year: 2012