Skip to main content Accessibility help
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 1
  • Print publication year: 2014
  • Online publication date: July 2014

16 - Using neuron specific RNAi inDrosophilafor understanding the molecular and neuronal basis of behavior


Abel, T. and Lattal, K.M. (2001) Molecular mechanisms of memory acquisition, consolidation and retrieval. Curr Opin Neurobiol 11(2): 180–187.
Adams, M.D., Celniker, S.E., Holt, R.A., et al. (2000) The genome sequence of Drosophila melanogaster. Science 287(5461): 218–295.
Alon, U. (2007) Network motifs: theory and experimental approaches. Nat Rev Genet 8(6): 450–461.
Ashburner, M., Ball, C.A., Blake, J.A., et al. (2000) Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 25(1): 25–29.
Bellen, H.J., Levis, R.W., Liao, G., et al. (2004) The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. Genetics 167(2): 761–781.
Bellen, H.J., Levis, R.W., He, Y., et al. (2011) The Drosophila gene disruption project: progress using transposons with distinctive site specificities. Genetics 188(3): 731–743.
Benzer, S. (1967) Behavioral mutants of Drosophila isolated by countercurrent distribution. Proc Natl Acad Sci USA 58(3): 1112–1119.
Bernstein, E., Caudy, A.A., Hammond, S.M., et al. (2001) Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409(6818): 363–366.
Birmingham, A., Selfors, L.M., Forster, T., et al. (2009) Statistical methods for analysis of high-throughput RNA interference screens. Nat Methods 6(8): 569–575.
Blair, S.S. (2003) Genetic mosaic techniques for studying Drosophila development. Development 130(21): 5065–5072.
Blenau, W. and Baumann, A. (2001) Molecular and pharmacological properties of insect biogenic amine receptors: Lessons from Drosophila melanogaster and Apis mellifera. Arch Insect Biochem Physiol 48(1): 13–38.
Bolduc, F.V., Bell, K., Cox, H., et al. (2008) Excess protein synthesis in Drosophila fragile X mutants impairs long-term memory. Nat Neurosci 11(10): 1143–1145.
Boutros, M. and J. Ahringer, J. (2008) The art and design of genetic screens: RNA interference. Nat Rev Genet 9(7): 554–566.
Brand, A.H. and Perrimon, N. (1993) Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118(2): 401–415.
Branson, K., Robie, A.A., Bender, J., et al. (2009) High-throughput ethomics in large groups of Drosophila. Nat Methods 6(6): 451–457.
Brent, M.R. (2008) Steady progress and recent breakthroughs in the accuracy of automated genome annotation. Nat Rev Genet 9(1): 62--73.
Bushey, D., Tononi, G., and Cirelli, C. (2009) The Drosophila fragile X mental retardation gene regulates sleep need. J Neurosci 29(7): 1948–1961.
Carpenter, A.E. and Sabatini, D.M. (2004) Systematic genome-wide screens of gene function. Nat Rev Genet 5(1): 11–22.
Carthew, R.W. and Sontheimer, E.J. (2009) Origins and mechanisms of miRNAs and siRNAs. Cell 136(4): 642–655.
Celniker, S.E., Dillon, L.A., Gerstein, M.B., et al. (2009) Unlocking the secrets of the genome. Nature 459(7249): 927–930.
Chapman, T., Bangham, J., Vinti, G., et al. (2003) The sex peptide of Drosophila melanogaster: female post-mating responses analyzed by using RNA interference. Proc Natl Acad Sci USA 100(17): 9923–9928.
Chen, S., Lee, A.Y., Bowens, N.M., et al. (2002) Fighting fruit flies: A model system for the study of aggression. Proc Natl Acad Sci USA 99(8): 5664–5668.
Chen, C.H., Huang, H., Ward, C.M., et al. (2007) A synthetic maternal-effect selfish genetic element drives population replacement in Drosophila. Science 316(5824): 597–600.
Chen, K.F., Peschel, N., Zavodska, R., et al. (2011) QUASIMODO, a Novel GPI-anchored zona pellucida protein involved in light input to the Drosophila circadian clock. Curr Biol 21(9): 719–729.
Chiu, J.C., Ko, H.W., and Edery, I. (2011) NEMO/NLK phosphorylates PERIOD to initiate a time-delay phosphorylation circuit that sets circadian clock speed. Cell 145(3): 357–370.
Chung, B.Y., Kilman, V.L., Keath, J.R., et al. (2009) The GABA(A) receptor RDL acts in peptidergic PDF neurons to promote sleep in Drosophila. Curr Biol 19(5): 386–390.
Copf, T., Goguel, V., Lampin-Saint-Amaux, A., et al. (2011) Cytokine signaling through the JAK/STAT pathway is required for long-term memory in Drosophila. Proc Natl Acad Sci USA 108(19): 8059–8064.
Czech, B. and Hannon, G.J. (2011) Small RNA sorting: matchmaking for Argonautes. Nat Rev Genet 12(1): 19–31.
Dankert, H., Wang, L., Hoopfer, E.D., et al. (2009) Automated monitoring and analysis of social behavior in Drosophila. Nat Methods 6(4): 297–303.
del Valle Rodriguez, A., Didiano, D., and Desplan, C. (2012) Power tools for gene expression and clonal analysis in Drosophila. Nat Methods 9(1): 47–55.
Dietzl, G., Chen, D., Schnorrer, F., et al. (2007) A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 448(7150): 151–156.
Dixon, S.J., Costanzo, M., Baryshnikova, A., et al. (2009) Systematic mapping of genetic interaction networks. Ann Rev Genet 43: 601–625.
Eddison, M., Guarnieri, D.J., Cheng, L., et al. (2011) arouser reveals a role for synapse number in the regulation of ethanol sensitivity. Neuron 70(5): 979–990.
Ejsmont, R.K., Sarov, M., Winkler, S., et al. (2009) A toolkit for high-throughput, cross-species gene engineering in Drosophila. Nat Methods 6(6): 435–437.
Faust, K., Gehrke, S., Yang, Y., et al. (2009) Neuroprotective effects of compounds with antioxidant and anti-inflammatory properties in a Drosophila model of Parkinson's disease. BMC Neurosci 10: 109.
Fedorov, Y., Anderson, E.M., Birmingham, A., et al. (2006) Off-target effects by siRNA can induce toxic phenotype. RNA 12(7): 1188–1196.
Feiguin, F., Godena, V.K., Romano, G., et al. (2009) Depletion of TDP-43 affects Drosophila motoneurons terminal synapsis and locomotive behavior. FEBS Lett 583(10): 1586–1592.
Fernandez, M.P., Chan, Y.B., Yew, J.Y., et al. (2010) Pheromonal and behavioral cues trigger male-to-female aggression in Drosophila. PLoS Biol 8(11): e1000541.
Fire, A., Xu, S., Montgomery, M.K., et al. (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391(6669): 806–811.
Flockhart, I.T., Booker, M., Hu, Y., et al. (2012) – the database of the Drosophila RNAi screening center: 2012 update. Nucl Acids Res 40 (Database issue): D715–D719.
Forslund, K., Schreiber, F., N. Thanintorn, N., et al. (2011) OrthoDisease: Tracking disease gene orthologs across 100 species. Briefings Bioinform 12(5): 463–473.
Fortier, E. and Belote, J.M. (2000) Temperature-dependent gene silencing by an expressed inverted repeat in Drosophila. Genesis 26(4): 240–244.
Gaj, T., C.A. Gersbach, C.A., and Barbas, C.F., C.F. ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends Biotechnol 2013.
Geschwind, D.H. and Konopka, G. (2009) Neuroscience in the era of functional genomics and systems biology. Nature 461(7266): 908–915.
Ghildiyal, M. and Zamore, P.D. (2009) Small silencing RNAs: An expanding universe. Nat Rev Genet 10(2): 94–108.
Giordano, E., Rendina, R., Peluso, I., et al. (2002) RNAi triggered by symmetrically transcribed transgenes in Drosophila melanogaster. Genetics 160(2): 637–648.
Golic, K.G. (1991) Site-specific recombination between homologous chromosomes in Drosophila. Science 252(5008): 958–961.
Gonzales, E. and Yin, J. (2010) Drosophila models of Huntington's disease exhibit sleep abnormalities. PLoS Curr 2.
Gouzi, J.Y., Moressis, A., Walker, J.A., et al. (2011) The receptor tyrosine kinase Alk controls neurofibromin functions in Drosophila growth and learning. PLoS Genet 7(9): e1002281.
Graveley, B.R., Brooks, A.N., Carlson, J.W., et al. (2011) The developmental transcriptome of Drosophila melanogaster. Nature 471(7339): 473–479.
Grigliatti, T.A., Hall, L., Rosenbluth, R., et al. (1973) Temperature-sensitive mutations in Drosophila melanogaster. XIV. A selection of immobile adults. Molec Gen Genet 120(2): 107–114.
Grishok, A., Pasquinelli, A.E., Conte, D., et al. (2001) Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing. Cell 106(1): 23–34.
Groth, A.C., Fish, M., Nusse, R., et al. (2004) Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Genetics 166(4): 1775–1782.
Groth, P., Weiss, B., Pohlenz, H.D., et al. (2008) Mining phenotypes for gene function prediction. BMC Bioinform 9: 136.
Haley, B., Hendrix, D., Trang, V., et al. (2008) A simplified miRNA-based gene silencing method for Drosophila melanogaster. Dev Biol 321(2): 482–490.
Haley, B., Foys, B., and Levine, M. (2010) Vectors and parameters that enhance the efficacy of RNAi-mediated gene disruption in transgenic Drosophila. Proc Natl Acad Sci USA 107(25): 11435–11440.
Hamada, F.N., Rosenzweig, M., Kang, K., et al. (2008) An internal thermal sensor controlling temperature preference in Drosophila. Nature 454(7201): 217–220.
Han, D.D., Stein, D., and Stevens, L.M. (2000) Investigating the function of follicular subpopulations during Drosophila oogenesis through hormone-dependent enhancer-targeted cell ablation. Development 127(3): 573–583.
Handler, D., Olivieri, D., Novatchkova, M., et al. (2011) A systematic analysis of Drosophila TUDOR domain-containing proteins identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway factors. EMBO J 30(19): 3977–3993.
Hansen, T.B., Jensen, T.I., Clausen, B.H., et al. (2013) Natural RNA circles function as efficient microRNA sponges. Nature 495(7441): 384–388.
Hasemeyer, M., Yapici, N., Heberlein, U., et al. (2009) Sensory neurons in the Drosophila genital tract regulate female reproductive behavior. Neuron 61(4): 511–518.
Horn, T., Arziman, Z., Berger, J., et al. (2007) GenomeRNAi: a database for cell-based RNAi phenotypes. Nucl Acids Res 35 (Database issue): D492–D497.
Horn, T., Sandmann, T., and Boutros, M. (2010) Design and evaluation of genome-wide libraries for RNA interference screens. Genome Biol 11(6): R61.
Horn, T., Sandmann, T., Fischer, B., et al. (2011) Mapping of signaling networks through synthetic genetic interaction analysis by RNAi. Nat Methods 8(4): 341–346.
Hutvagner, G., McLachlan, J., Pasquinelli, A.E., et al. (2001) A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 293(5531): 834–838.
Ishimoto, H., Sakai, T., and Kitamoto, T. (2009) Ecdysone signaling regulates the formation of long-term courtship memory in adult Drosophila melanogaster. Proc Natl Acad Sci USA 106(15): 6381–6386.
Jackson, A.L., Burchard, J., Schelter, J., et al. (2006) Widespread siRNA “off-target” transcript silencing mediated by seed region sequence complementarity. RNA 12(7): 1179–1187.
Jenett, A., Rubin, G.M., Ngo, T.T., et al. (2012) A GAL4-driver line resource for Drosophila neurobiology. Cell Rep 2(4): 991–1001.
Jepson, J.E. and Reenan, R.A. (2009) Adenosine-to-inosine genetic recoding is required in the adult stage nervous system for coordinated behavior in Drosophila. J Biol Chem 284(45): 31391–31400.
Kain, P., Badsha, F., Hussain, S.M., et al. (2010) Mutants in phospholipid signaling attenuate the behavioral response of adult Drosophila to trehalose. Chem Senses 35(8): 663–673.
Kalidas, S. and Smith, D.P. (2002) Novel genomic cDNA hybrids produce effective RNA interference in adult Drosophila. Neuron 33(2): 177–184.
Kanehisa, M., Goto, S., Sato, Y., et al. (2012) KEGG for integration and interpretation of large-scale molecular data sets. Nucl Acids Res 40 (Database issue): D109–D114.
Kang, J., Kim, J., and Choi, K.W. (2011) Novel cytochrome P450, cyp6a17, is required for temperature preference behavior in Drosophila. PLoS One 6(12): e29800.
Kaplow, I.M., Singh, R., Friedman, A., et al. (2009) RNAiCut: Automated detection of significant genes from functional genomic screens. Nat Methods 6(7): 476–467.
Kaupp, U.B. (2010) Olfactory signalling in vertebrates and insects: Differences and commonalities. Nat Rev Neurosci 11(3): 188–200.
Kennerdell, J.R. and Carthew, R.W. (2000) Heritable gene silencing in Drosophila using double-stranded RNA. Nat Biotechnol 18(8): 896–898.
Keleman, K., Micheler, T., and VDRC project members (2009) RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center (FBrf0208510). Pers Comm FlyBase 2009.
Ketting, R.F., Fischer, S.E., Bernstein, E., et al. (2001) Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes Dev 15(20): 2654–2659.
King, I., Tsai, L.T., Pflanz, R., et al. (2011) Drosophila tao controls mushroom body development and ethanol-stimulated behavior through par-1. J Neurosci 31(3): 1139–1148.
Kim, V.N., Han, J., and Siomi, M.C. (2009) Biogenesis of small RNAs in animals. Nat Rev Molec Cell Biol 10(2): 126–139.
Knight, S.W. and Bass, B.L. (2001) A role for the RNase III enzyme DCR-1 in RNA interference and germ line development in Caenorhabditis elegans. Science 293(5538): 2269–2271.
Kondo, S., Booker, M., and Perrimon, N. (2009) Cross-species RNAi rescue platform in Drosophila melanogaster. Genetics 183(3): 1165–1173.
Kondo, T., Inagaki, S., Yasuda, K., et al. (2006) Rapid construction of Drosophila RNAi transgenes using pRISE, a P-element-mediated transformation vector exploiting an in vitro recombination system. Genes Genet Syst 81(2): 129–134.
Konopka, R.J. and Benzer, S. (1971) Clock mutants of Drosophila melanogaster. Proc Natl Acad Sci USA 68(9): 2112–2116.
Krashes, M.J., DasGupta, S., Vreede, A., et al. (2009) A neural circuit mechanism integrating motivational state with memory expression in Drosophila. Cell 139(2): 416–427.
Krastev, D.B., Slabicki, M., Paszkowski-Rogacz, M., et al. (2011) A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly. Nature Cell Biol 13(7): 809–818.
Kulkarni, M.M., Booker, M., Silver, S.J., et al. (2006) Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays. Nat Methods 3(10): 833–838.
Ladoukakis, E., Pereira, V., Magny, E.G., et al. (2011) Hundreds of putatively functional small open reading frames in Drosophila. Genome Biol 12(11): R118.
Lam, G. and Thummel, C.S. (2000) Inducible expression of double-stranded RNA directs specific genetic interference in Drosophila. Curr Biol 10(16): 957–963.
Langer, C.C., Ejsmont, R.K., Schonbauer, C., et al. (2010) In vivo RNAi rescue in Drosophila melanogaster with genomic transgenes from Drosophila pseudoobscura. PLoS One 5(1): e8928.
Lee, K.S., You, K.H., Choo, J.K., et al. (2004) Drosophila short neuropeptide F regulates food intake and body size. J Biol Chem 279(49): 50781–50789.
Lee, P.T., Lin, H.W., Chang, Y.H., et al. (2011) Serotonin-mushroom body circuit modulating the formation of anesthesia-resistant memory in Drosophila. Proc Natl Acad Sci USA 108(33): 13794–13799.
Lee, T., Winter, C., Marticke, S.S., et al. (2000) Essential roles of Drosophila RhoA in the regulation of neuroblast proliferation and dendritic but not axonal morphogenesis. Neuron 25(2): 307–316.
Lee, Y.S. and Carthew, R.W. (2003) Making a better RNAi vector for Drosophila: Use of intron spacers. Nat Methods 30(4): 322–329.
Lee, Y.S., Nakahara, K., Pham, J.W., et al. (2004) Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell 117(1): 69–81.
Lehner, B., Crombie, C., Tischler, J., et al. (2006) Systematic mapping of genetic interactions in Caenorhabditis elegans identifies common modifiers of diverse signaling pathways. Nat Genet 38(8): 896–903.
Lessing, D. and Bonini, N.M. (2009) Maintaining the brain: Insight into human neurodegeneration from Drosophila melanogaster mutants. Nat Rev Genet 10(6): 359–360.
Li, Y., Hoxha, V., Lama, C., et al. (2011) The hector G-protein coupled receptor is required in a subset of fruitless neurons for male courtship behavior. PLoS One 6(11): e28269.
Lim, C., Chung, B.Y., Pitman, J.L., et al. (2007) Clockwork orange encodes a transcriptional repressor important for circadian-clock amplitude in Drosophila. Curr Biol 17(12): 1082–1089.
Lim, C., Lee, J., Choi, C., et al. (2011) The novel gene twenty-four defines a critical translational step in the Drosophila clock. Nature 470(7334): 399–403.
Lin, M.J., Cheng, C.W., and Shen, C.K. (2011) Neuronal function and dysfunction of Drosophila dTDP. PLoS One 6(6): e20371.
Liu, X., Krause, W.C., and Davis, R.L. (2007) GABAA receptor RDL inhibits Drosophila olfactory associative learning. Neuron 56(6): 1090–1102.
Luo, W. and Sehgal, A. (2012) Regulation of circadian behavioral output via a microRNA-JAK/STAT circuit. Cell 148(4): 765–779.
Lyne, R., Smith, R., Rutherford, K., et al. (2007) FlyMine: an integrated database for Drosophila and Anopheles genomics. Genome Biol 8(7): R129.
Ma, Y., Creanga, A., Lum, L., et al. (2006) Prevalence of off-target effects in Drosophila RNA interference screens. Nature 443(7109): 359–363.
McGuire, S.E., Le, P.T., Osborn, A.J., et al. (2003) Spatiotemporal rescue of memory dysfunction in Drosophila. Science 302(5651): 1765–1768.
McQuibban, A.G., Joza, N., Megighian, A., et al. (2010) A Drosophila mutant of LETM1, a candidate gene for seizures in Wolf–Hirschhorn syndrome. Hum Molec Genet 19(6): 987–1000.
Manoli, D.S., Foss, M.A., Villella, M.A., et al. (2005) Male-specific fruitless specifies the neural substrates of Drosophila courtship behaviour. Nature 436(7049): 395–400.
Margulies, C., Tully, T., and Dubnau, J. (2005) Deconstructing memory in Drosophila. Curr Biol 15(17): R700–R713.
Martinek, S. and Young, M.W. (2000) Specific genetic interference with behavioral rhythms in Drosophila by expression of inverted repeats. Genetics 156(4): 1717–1725.
Matsumoto, A., Ukai-Tadenuma, M., Yamada, R.G., et al. (2007) A functional genomics strategy reveals clockwork orange as a transcriptional regulator in the Drosophila circadian clock. Genes Dev 21(13): 1687–1700.
Meissner, G.W., Manoli, D.S., Chavez, J.F., et al. (2011) Functional dissection of the neural substrates for sexual behaviors in Drosophila melanogaster. Genetics 189(1): 195–211.
Melicharek, D.J., Ramirez, L.C., Singh, S., et al. (2010) Kismet/CHD7 regulates axon morphology, memory and locomotion in a Drosophila model of CHARGE syndrome. Hum Molec Genet 19(21): 4253–4264.
Memczak, S., Jens, M., Elefsinioti, A., et al. (2013) Circular RNAs are a large class of animal RNAs with regulatory potency. Nature 495(7441): 333–338.
Moffat, J., Reiling, J.H., and D.M. Sabatini, D.M. (2007) Off-target effects associated with long dsRNAs in Drosophila RNAi screens. Trends Pharmacol Sci 28(4): 149–451.
Mohr, S., Bakal, C., Perrimon, N. (2010) Genomic screening with RNAi: Results and challenges. Ann Rev Biochem 79: 37--64.
Montgomery, M.K., Xu, S., and Fire, A. (1998) RNA as a target of double- stranded RNA-mediated genetic interference in Caenorhabditis elegans. Proc Natl Acad Sci USA 95(26): 15502–15507.
Mummery-Widmer, J.L., Yamazaki, M., Stoeger, T., et al. (2009) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Nature 458(7241): 987--992.
Nagoshi, E., Sugino, K., Kula, E., et al. (2010) Dissecting differential gene expression within the circadian neuronal circuit of Drosophila. Nat Neurosci 13(1): 60–68.
Nedelsky, N.B., Pennuto, M., Smith, R.B., et al. (2010) Native functions of the androgen receptor are essential to pathogenesis in a Drosophila model of spinobulbar muscular atrophy. Neuron 67(6): 936–952.
Neely, G.G., A. Hess, A., Costigan, M., et al. (2010) A genome-wide Drosophila screen for heat nociception identifies alpha2delta3 as an evolutionarily conserved pain gene. Cell 143(4): 628–638.
Neely, G.G., Kuba, K., Cammarato, A., et al. (2010) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Cell 141(1): 142–153.
Negre, N., Brown, C.D., Ma, L., et al. (2011) A cis-regulatory map of the Drosophila genome. Nature 471(7339): 527–531.
Ni, J.Q., Markstein, M., Binari, R., et al. (2008) Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. Nat Methods 5(1): 49–51.
Ni, J.Q., Liu, L.P., Binari, R., et al. (2009) A Drosophila resource of transgenic RNAi lines for neurogenetics. Genetics 182(4): 1089–1100.
Ni, J.Q., Zhou, R., Czech, B., et al. (2011) A genome-scale shRNA resource for transgenic RNAi in Drosophila. Nat Methods 8(5): 405–407.
Orso, G., Martinuzzi, A., Rossetto, M.G., et al. (2005) Disease-related phenotypes in a Drosophila model of hereditary spastic paraplegia are ameliorated by treatment with vinblastine. J Clin Invest 115(11): 3026–3034.
Osterwalder, T., Yoon, K.S., White, B.H., et al. (2001) A conditional tissue-specific transgene expression system using inducible GAL4. Proc Natl Acad Sci 98(22): 12596–12601.
Pak, C., Garshasbi, M., Kahrizi, K., et al. (2011) Mutation of the conserved polyadenosine RNA binding protein, ZC3H14/dNab2, impairs neural function in Drosophila and humans. Proc Natl Acad Sci USA 108(30): 12390–12395.
Pauli, A., Althoff, F., Oliveira, R.A., et al. (2008) Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons. Dev Cell 14(2): 239–251.
Perrimon, N., Ni, J.Q., and Perkins, L. (2010) In vivo RNAi: today and tomorrow. Cold Spring Harbor Perspectives Biol 2(8): a003640.
Piccin, A., Salameh, A., Benna, C., et al. (2001) Efficient and heritable functional knock-out of an adult phenotype in Drosophila using a GAL4-driven hairpin RNA incorporating a heterologous spacer. Nucl Acids Res 29(12): E55–55.
Pick, S. and R. Strauss, R. (2005) Goal-driven behavioral adaptations in gap-climbing Drosophila. Curr Biol 15(16): 1473–1478.
Pili-Floury, S., Leulier, F., Takahashi, K., et al. (2004) In vivo RNA interference analysis reveals an unexpected role for GNB P1 in the defense against Gram-positive bacterial infection in Drosophila adults. J Biol Chem 279(13): 12848–12853.
Piomelli, D. (2003) The molecular logic of endocannabinoid signalling. Nat Rev Neurosci 4(11): 873–884.
Pospisilik, J.A., Schramek, D., Schnidar, H., et al. (2010) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Cell 140(1): 148–160.
Przytycka, T.M., Singh, M., and Slonim, D.K. (2011) Toward the dynamic interactome: it's about time. Briefings Bioinformatics 11(1): 15–29.
Reichhart, J.M., Ligoxygakis, P., Naitza, S., et al. (2002) Splice-activated UAS hairpin vector gives complete RNAi knockout of single or double target transcripts in Drosophila melanogaster. Genesis 34(1–2): 160–164.
Ribeiro, C. and B.J. Dickson, B.J. (2010) Sex peptide receptor and neuronal TOR/S6K signaling modulate nutrient balancing in Drosophila. Curr Biol 20(11): 1000–1005.
Roman, G. and Davis, R.L. (2002) Conditional expression of UAS-transgenes in the adult eye with a new gene-switch vector system. Genesis 34(1–2): 127–131.
Roman, G., Endo, K., Zong, L., et al. (2001) P[Switch], a system for spatial and temporal control of gene expression in Drosophila melanogaster. Proc Natl Acad Sci 98(22): 12602–12607.
St Johnston, D. (2002) The art and design of genetic screens: Drosophila melanogaster. Nat Rev Genet 3(3): 176–188.
Schnorrer, F., Schonbauer, C., Langer, C.C., et al. (2010) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Nature 464(7286): 287–291.
Schumann, G., Coin, L.J., Lourdusamy, A., et al. (2011) Genome-wide association and genetic functional studies identify autism susceptibility candidate 2 gene (AUTS2) in the regulation of alcohol consumption. Proc Natl Acad Sci USA 108(17): 7119–7124.
Scott, M.M., Krueger, K.C., and Deneris, E.S. (2005) A differentially autoregulated Pet-1 enhancer region is a critical target of the transcriptional cascade that governs serotonin neuron development. J Neurosci 25(10): 2628–2636.
Schulz, J.G., G. David, G., and Hassan, B.A. (2009) A novel method for tissue-specific RNAi rescue in Drosophila. Nucl Acids Res 37(13): p. e93.
Sepp, K.J., Hong, P., Lizarraga, S.B., et al. (2008) Identification of neural outgrowth genes using genome-wide RNAi. PLoS Genet 4(7): e1000111.
Shimizu, H., Shimoda, M., Yamaguchi, T., et al. (2008) Drosophila ATF-2 regulates sleep and locomotor activity in pacemaker neurons. Molec Cell Biol 28(20): 6278–6289.
Siddiqi, O. and Benzer, S. (1976) Neurophysiological defects in temperature-sensitive paralytic mutants of Drosophila melanogaster. Proc Natl Acad Sci USA 73(9): 3253–3257.
Singh, N., Lorbeck, M.T., Zervos, A., et al. (2010) The histone acetyltransferase Elp3 plays in active role in the control of synaptic bouton expansion and sleep in Drosophila. J Neurochem 115(2): 493–504.
Sitaraman, D., Zars, M., Laferriere, H., et al. (2008) Serotonin is necessary for place memory in Drosophila. Proc Natl Acad Sci USA 105(14): 5579–5584.
Starz-Gaiano, M., Cho, N.K., Forbes, A., et al. (2001) Spatially restricted activity of a Drosophila lipid phosphatase guides migrating germ cells. Development 128(6): 983–991.
Stavropoulos, N. and Young, M.W. (2011) insomniac and Cullin-3 regulate sleep and wakefulness in Drosophila. Neuron 72(6): 964–976.
Stielow, B., Sapetschnig, A., Kruger, I., et al. (2008) Identification of SUMO-dependent chromatin-associated transcriptional repression components by a genome-wide RNAi screen. Molec Cell 29(6): 742–754.
Stockinger, P., Kvitsiani, D., Rotkopf, S., et al. (2005) Neural circuitry that governs Drosophila male courtship behavior. Cell 121(5): 795–807.
Strauss, R. and Heisenberg, M. (1993) A higher control center of locomotor behavior in the Drosophila brain. J Neurosci 13(5): 1852–1861.
Sokolowski, M.B. (2001) Drosophila: Genetics meets behaviour. Nat Rev Genet 2(11): 879–890.
Takahama, K., Tomita, J., Ueno, T., et al. (2012) Pan-neuronal knockdown of the c-Jun N-terminal Kinase (JNK) results in a reduction in sleep and longevity in Drosophila. Biochem Biophys Res Commun 417(2): 807–811.
Tomita, J., Mitsuyoshi, M., Ueno, T., et al. (2011) Pan-neuronal knockdown of calcineurin reduces sleep in the fruit fly, Drosophila melanogaster. J Neurosci 31(37): 13137–13146.
Tong, A.H., Lesage, G., Bader, G.D., et al. (2004) Global mapping of the yeast genetic interaction network. Science 303(5659): 808–813.
Ueda, H.R., Matsumoto, A., Kawamura, M., et al. (2002) Genome-wide transcriptional orchestration of circadian rhythms in Drosophila. J Biol Chem 277(16): 14048–14052.
Vagin, V.V., Sigova, A., Li, C., et al. (2006) A distinct small RNA pathway silences selfish genetic elements in the germline. Science 313(5785): 320–324.
Venken, K.J., Simpson, J.H., and Bellen, H.J. (2011) Genetic manipulation of genes and cells in the nervous system of the fruit fly. Neuron 72(2): 202–230.
Vert, J.P., Foveau, N., Lajaunie, C., et al. (2006) An accurate and interpretable model for siRNA efficacy prediction. BMC Bioinform 7: 520.
Vinayagam, A., Hu, Y., Kulkarni, M., et al. (2013) Protein complex-based analysis framework for high-throughput data sets. Sci Signaling 6(264): rs5.
Vosshall, L.B. (2007) Into the mind of a fly. Nature 450(7167): 193–197.
Wang, S.H. and Elgin, S.C. (2011) Drosophila Piwi functions downstream of piRNA production mediating a chromatin-based transposon silencing mechanism in female germ line. Proc Natl Acad Sci USA 108(52): 21164–21169.
Warde-Farley, D., Donaldson, S.L., Comes, A., et al. (2010) The GeneMANIA prediction server: biological network integration for gene prioritization and predicting gene function. Nucl Acids Res 38 (Web Server issue): W214–W220.)
Wu, Q., Zhao, Z., and Shen, P. (2005) Regulation of aversion to noxious food by Drosophila neuropeptide Y- and insulin-like systems. Nat Neurosci 8(10): 1350–1355.
Wu, C.L., Xia, S., Fu, T.F., et al. (2007) Specific requirement of NMDA receptors for long-term memory consolidation in Drosophila ellipsoid body. Nat Neurosci 10(12): 1578–1586.
Xia, S. and Tully, T. (2007) Segregation of odor identity and intensity during odor discrimination in Drosophila mushroom body. PLoS Biol 5(10): e264.
Yapici, N., Kim, Y.J., Ribeiro, C., et al. (2008) A receptor that mediates the post-mating switch in Drosophila reproductive behaviour. Nature 451(7174): 33–37.
Yarmolinsky, D.A., Zuker, C.S., and Ryba, N.J. (2009) Common sense about taste: From mammals to insects. Cell 139(2): 234–244.
Yu, W. and Hardin, P.E. (2006) Circadian oscillators of Drosophila and mammals. J Cell Sci 119(23): 4793–4795.
Yu, W., Houl, J.H., and Hardin, P.E. (2011) NEMO kinase contributes to core period determination by slowing the pace of the Drosophila circadian oscillator. Curr Biol 21(9): 756–761.
Zhong, L., Hwang, R.Y., and Tracey, W.D. (2010) Pickpocket is a DEG/ENaC protein required for mechanical nociception in Drosophila larvae. Curr Biol 20(5): 429–434.