Depression is one of the major mental disorders, which seriously endangers human health, brings a serious burden to patients’ families. In this study, we intended to further explore the antidepressant-like effect and possible molecular mechanisms of Salidroside (SAL). We built corticosterone (CORT)-induced depressive mice model and used behavioural tests to evaluate depression behaviour. To explore the molecular mechanisms of SAL, we employed a variety of methods such as immunofluorescence, western blot, pharmacological interference, etc. The results demonstrated that SAL both at 25 mg/kg and 50 mg/kg can reduce immobility time in the tail suspension test (TST). At the same time, SAL treatment could restore the reduced sugar water intake preference in the sucrose preference test (SPT) in CORT-induced depressive mice and reduce the immobility time in TST and forced swimming experiments (FST). In addition, SAL treatment reversed the reduction in the number of Ki-67, BrdU, and NeuN in the hippocampus due to CORT treatment. SAL treatment also restored the expression of SIRT1, PGC-1α, brain-derived neurotrophic factor (BDNF) and other proteins in the hippocampus. In addition, after blocking SIRT1 signalling with EX527, we found that the treatment with SAL failed to reduce the immobility time in TST and FST, the level of SIRT1 and PGC-1α activity were correspondingly downregulated, and the expression of DCX and Ki-67 in the hippocampus failed to be activated. These findings suggested that SAL exerts antidepressant-like effects by promoting hippocampal neurogenesis through the SIRT1/PGC-1α signalling pathway.

Let G and H be two vertex disjoint graphs. The join $G+H$ is the graph with $V(G+H)=V(G)+V(H)$ and $E(G+H)=E(G)\cup E(H)\cup \{xy\;|\; x\in V(G), y\in V(H)\}$. A (finite) linear forest is a graph consisting of (finite) vertex disjoint paths. We prove that for any finite linear forest F and any nonnull graph H, if $\{F, H\}$-free graphs have a $\chi $-binding function $f(\omega )$, then $\{F, K_n+H\}$-free graphs have a $\chi $-binding function $kf(\omega )$ for some constant k.

Thanks to its real-time computation efficiency, deep reinforcement learning (DRL) has been widely applied in motion planning for mobile robots. In DRL-based methods, a DRL model computes an action for a robot based on the states of its surrounding obstacles, including other robots that may communicate with it. These methods always assume that the environment is attack-free and the obtained obstacles’ states are reliable. However, in the real world, a robot may suffer from obstacle localization attacks (OLAs), such as sensor attacks, communication attacks, and remote-control attacks, which cause the robot to retrieve inaccurate positions of the surrounding obstacles. In this paper, we propose a robust motion planning method ObsGAN-DRL, integrating a generative adversarial network (GAN) into DRL models to mitigate OLAs in the environment. First, ObsGAN-DRL learns a generator based on the GAN model to compute the approximation of obstacles’ accurate positions in benign and attack scenarios. Therefore, no detectors are required for ObsGAN-DRL. Second, by using the approximation positions of the surrounding obstacles, ObsGAN-DRL can leverage the state-of-the-art DRL methods to compute collision-free motion commands (e.g., velocity) efficiently. Comprehensive experiments show that ObsGAN-DRL can mitigate OLAs effectively and guarantee safety. We also demonstrate the generalization of ObsGAN-DRL.

In order to gain a better understanding of clay and Fe (oxyhydr)oxide minerals formed during pedogenesis of basalts in tropical monsoonal Hainan (southern China), a basalt-derived lateritic soil at Nanyang, Hainan, was investigated comprehensively. The results show that the lateritic regolith consists uniformly of kaolinite and Fe (oxyhydr)oxide minerals, with trace gibbsite only in the AE horizon. Abundant dioctahedral smectite in the basalt bedrock formed due to primary hydrothermal alteration, and transformed to kaolinite rapidly in the highly weathering saprolite horizon. The ‘crystallinity’ of kaolinite is notably low and its Hinckley index fluctuates along the soil profile, resulting from intense ferrolysis due to fluctuations between wet/dry climate conditions. From the base to the top of the profile, maghemite shows a decreasing trend, whereas magnetite, hematite, and goethite exhibit a slightly increasing trend, indicating that maghemite formed as an initial product of basalt weathering. Formation of Fe (oxyhydr)oxide species in basalt-derived soil is mainly controlled by local environmental conditions such as soil moisture, redox, and acidic conditions; thus, iron mineral-based paleoclimatic proxies could not be used for subtropical to tropical soils. The highly weathered saprolite has a similar δ56Fe value (+0.06‰) to that (+0.07‰) of the parent rock, while the AE to middle E horizons have greater δ56Fe values of +0.12‰ to +0.19‰. Fe isotopic signatures correlate positively with the Fe mass transfer coefficient (R2=0.77, n=6, ρ<0.05), indicating repetitive weathering and relative accumulation of isotopically heavier Fe in the upper soil horizons, which occurred by reductive dissolution of organic matter under oxic conditions, as reflected by the greater U/Th.

Background: Chronic subdural hematoma (CSDH) is a common neurosurgical condition which can be treated with surgical evacuation. A significant percentage of CSDH patients are on antiplatelet or anticoagulation therapy at baseline which may influence risk of recurrence and postoperative thromboembolic events Methods: A search was conducted in MEDLINE (1946 to April 6, 2023), Embase (1974 to April 6, 2023), and PubMed (up to April 6, 2023) on preoperative use of antiplatelet or anticoagulation therapy and outcomes following surgical evacuation of CSDH. Results: Our literature includes 14,410 patients ifrom 42 studies, with 3218 (22%) in the antiplatelet (AP) group, 1731(12%) in the anticoagulation (AC) group, and 9537 (66%) in the no antithrombotics (NA) group. The AP group had significantly higher recurrence compared to NA (OR = 1.21, 95% CI = 1.04 to 1.40, p = 0.01). The AC group also had significantly high recurrence compared to NA (OR = 1.39. 95% CI = 1.15 to 1.68, p = 0.0007). However, being on any antithrombotic therapy is also associated with significantly higher thromboembolic events (OR 5.41, 95% CI 3.16 to 9.26, p < 0.00001). Conclusions: Patients on antithrombotic therapy have both higher recurrence and higher thromboembolic risk compared to patients not on antithrombotic therapy.

Background: The fragility index (FI) is the minimum number of patients whose status would have to change from a nonevent to an event to turn a statistically significant result to a non-significant result. We used this to measure the robustness of trials comparing carotid endarterectomy (CEA) to carotid artery stenting (CAS). Methods: A search was conducted in MEDLINE, Embase, and PubMed on RCTs comparing CEA to CAS. The trials need to have statistically significant results and dichotomous primary endpoints to be included. Results: Our literature search identified 10 RCTs which included 9382 patients (4734 CEA, 4648 CAS). The primary end points of all included trials favoured CEA over CAS. The median FI was 9.5 (interquartile range 2.25 - 21.25). All of the studies that reported lost-to-follow-up (LTFU) had LTFU greater than its fragility index, which raises concern that the missing data could change the results of the trial from statistically significant to statistically insignificant. Conclusions: A small number of events (FI, median 9.5) were required to render the results of carotid artery stenosis RCTs comparing CEA to CAS statistically insignificant. All of the studies that reported LTFU had LTFU greater than its fragility index.

China is still among the 30 high-burden tuberculosis (TB) countries in the world. Few studies have described the spatial epidemiological characteristics of pulmonary TB (PTB) in Jiangsu Province. The registered incidence data of PTB patients in 95 counties of Jiangsu Province from 2011 to 2021 were collected from the Tuberculosis Management Information System. Three-dimensional spatial trends, spatial autocorrelation, and spatial–temporal scan analysis were conducted to explore the spatial clustering pattern of PTB. From 2011 to 2021, a total of 347,495 newly diagnosed PTB cases were registered. The registered incidence rate of PTB decreased from 49.78/100,000 in 2011 to 26.49/100,000 in 2021, exhibiting a steady downward trend (χ2 = 414.22, P < 0.001). The average annual registered incidence rate of PTB was higher in the central and northern regions. Moran’s I indices of the registered incidence of PTB were all >0 (P< 0.05) except in 2016, indicating a positive spatial correlation overall. Local autocorrelation analysis showed that ‘high–high’ clusters were mainly distributed in northern Jiangsu, and ‘low–low’ clusters were mainly concentrated in southern Jiangsu. The results of this study assist in identifying settings and locations of high TB risk and inform policy-making for PTB control and prevention.

This paper retrospectively analysed the prevalence of macrolide-resistant Mycoplasma pneumoniae (MRMP) in some parts of China. Between January 2013 and December 2019, we collected 4,145 respiratory samples, including pharyngeal swabs and alveolar lavage fluid. The highest PCR-positive rate of M. pneumoniae was 74.5% in Beijing, the highest resistance rate was 100% in Shanghai, and Gansu was the lowest with 20%. The highest PCR-positive rate of M. pneumoniae was 74.5% in 2013, and the highest MRMP was 97.4% in 2019; the PCR-positive rate of M. pneumoniae for adults in Beijing was 17.9% and the MRMP was 10.48%. Among the children diagnosed with community-acquired pneumonia (CAP), the PCR-positive and macrolide-resistant rates of M. pneumoniae were both higher in the severe ones. A2063G in domain V of 23S rRNA was the major macrolide-resistant mutation, accounting for more than 90%. The MIC values of all MRMP to erythromycin and azithromycin were ≥ 64 μg/ml, and the MICs of tetracycline and levofloxacin were ≤ 0.5 μg/ml and ≤ 1 μg/ml, respectively. The macrolide resistance varied in different regions and years. Among inpatients, the macrolide-resistant rate was higher in severe pneumonia. A2063G was the common mutation, and we found no resistance to tetracycline and levofloxacin.

Hearts of cotorsion pairs on extriangulated categories are abelian categories. On the other hand, hearts of twin cotorsion pairs are not always abelian. They were shown to be semi-abelian by Liu and Nakaoka. Moreover, Hassoun and Shah proved that they are quasi-abelian under certain conditions. In this article, we first show that the heart of any twin cotorsion pair has a largest exact category structure and is always quasi-abelian. We also provide a sufficient and necessary condition for the heart of a twin cotorsion pair being abelian. Then by using the results we have got, we investigate the almost split sequences in the hearts of twin cotorsion pairs. Finally, as an application, we show that a Krull–Schmidt, Hom-finite triangulated category has a Serre functor whenever it has a cluster tilting object.

Oocytes with excessively large first polar bodies (PB1) often occur in assisted reproductive procedures. Many times these oocytes are discarded without insemination and, as a result, the application of this portion of oocytes has scarcely been reported to date. Few studies have examined large PB1 oocytes in infertile women and have virtually entirely studied genetic variations for large PB1 oocyte abnormalities. Here, we describe an unusual case of a live birth from a remarkably large PB1 oocyte in a frozen embryo transfer (FET) cycle. This is the first instance of a successful live birth resulting from a PB1 oocyte with an extremely large polar body measuring 80 μM × 40 μM in size. The large PB1 oocyte was performed by an early rescue intracytoplasmic sperm injection (r-ICSI) and was formed into a blastocyst on day 5. Following FET, a healthy boy baby weighing 3100 g was finally delivered by caesarean section at 37 weeks and 5 days after conception. Additionally, there were no complications throughout the antenatal period or the perinatal phase of this following full-term delivery. In this study, it is revealed for the first time that a huge PB1 oocyte can be fertilized, resulting in the growth of a blastocyst, a subsequent pregnancy, and a live birth. This new information prompts us to reconsider the use of large PB1 oocytes. More insightful talks should be given attention to prevent the waste of embryos because not all oocytes with aberrant morphology are unavailable.

Soft drink consumption has become a highly controversial public health issue. Given the pattern of consumption in China, sugar-sweetened beverage is the main type of soft drink consumed. Due to containing high levels of fructose, a soft drink may have a deleterious effect on handgrip strength (HGS) due to oxidative stress, inflammation and insulin resistance. However, few studies show an association between soft drink consumption and HGS in adults. We aimed to investigate the association between soft drink consumption and longitudinal changes in HGS among a Chinese adult population. A longitudinal population-based cohort study (5-year follow-up, median: 3·66 years) was conducted in Tianjin, China. A total of 11 125 participants (56·7 % men) were enrolled. HGS was measured using a handheld digital dynamometer. Soft drink consumption (mainly sugar-containing carbonated beverages) was measured at baseline using a validated FFQ. ANCOVA was used to evaluate the association between soft drink consumption and annual change in HGS or weight-adjusted HGS. After adjusting for multiple confounding factors, the least square means (95 % CI) of annual change in HGS across soft drink consumption frequencies were −0·70 (–2·49, 1·09) for rarely drinks, −0·82 (–2·62, 0·97) for < 1 cup/week and −0·86 (–2·66, 0·93) for ≥ 1 cup/week (Pfor trend < 0·05). Likewise, a similar association was observed between soft drink consumption and annual change in weight-adjusted HGS. The results indicate that higher soft drink consumption was associated with faster HGS decline in Chinese adults.

Cryo-electron microscopy (cryo-EM) is an imaging technique that allows the visualization of proteins and macromolecular complexes at near-atomic resolution. The low electron doses used to prevent radiation damage to the biological samples result in images where the power of noise is 100 times stronger than that of the signal. Accurate identification of proteins from these low signal-to-noise ratio (SNR) images is a critical task, as the detected positions serve as inputs for the downstream 3D structure determination process. Current methods either fail to identify all true positives or result in many false positives, especially when analyzing images from smaller-sized proteins that exhibit extremely low contrast, or require manual labeling that can take days to complete. Acknowledging the fact that accurate protein identification is dependent upon the visual interpretability of micrographs, we propose a framework that can perform denoising and detection in a joint manner and enable particle localization under extremely low SNR conditions using self-supervised denoising and particle identification from sparsely annotated data. We validate our approach on three challenging single-particle cryo-EM datasets and projection images from one cryo-electron tomography dataset with extremely low SNR, showing that it outperforms existing state-of-the-art methods used for cryo-EM image analysis by a significant margin. We also evaluate the performance of our algorithm under decreasing SNR conditions and show that our method is more robust to noise than competing methods.

The numerical investigation focuses on the flow patterns around a rectangular cylinder with three aspect ratios ($L/D=5$, $10$, $15$) at a Reynolds number of $1000$. The study delves into the dynamics of vortices, their associated frequencies, the evolution of the boundary layer and the decay of the wake. Kelvin–Helmholtz (KH) vortices originate from the leading edge (LE) shear layer and transform into hairpin vortices. Specifically, at $L/D=5$, three KH vortices merge into a single LE vortex. However, at $L/D=10$ and $15$, two KH vortices combine to form a LE vortex, with the rapid formation of hairpin vortex packets. A fractional harmonic arises due to feedback from the split LE shear layer moving upstream, triggering interaction with the reverse flow. Trailing edge (TE) vortices shed, creating a Kármán-like street in the wake. The intensity of wake oscillation at $L/D=5$ surpasses that in the other two cases. Boundary layer transition occurs after the saturation of disturbance energy for $L/D=10$ and $15$, but not for $L/D=5$. The low-frequency disturbances are selected to generate streaks inside the boundary layer. The TE vortex shedding induces the formation of a favourable pressure gradient, accelerating the flow and fostering boundary layer relaminarization. The self-similarity of the velocity defect is observed in all three wakes, accompanied by the decay of disturbance energy. Importantly, the decrease in the shedding frequency of LE (TE) vortices significantly contributes to the overall decay of disturbance energy. This comprehensive exploration provides insights into complex flow phenomena and their underlying dynamics.

The current LiDAR-inertial odometry is prone to cumulative Z-axis error when it runs for a long time. This error can easily lead to the failure to detect the loop-closing in the correct scenario. In this paper, a ground-constrained LiDAR-inertial SLAM is proposed to solve this problem. Reasonable constraints on the ground motion of the mobile robot are incorporated to limit the Z-axis drift error. At the same time, considering the influence of initial positioning error on navigation, a keyframe selection strategy is designed to effectively improve the flatness and accuracy of positioning and the efficiency of loop detection. If GNSS is available, the GNSS factor is added to eliminate the cumulative error of the trajectory. Finally, a large number of experiments are carried out on the self-developed robot platform to verify the effectiveness of the algorithm. The results show that this method can effectively improve location accuracy in outdoor environments, especially in environments of feature degradation and large scale.

Cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) is a distinctive member of the serine–threonine protein AGC kinase family and an effective kinase for cAMP signal transduction. In recent years, scuticociliate has caused a lot of losses in domestic fishery farming, therefore, we have carried out morphological and molecular biological studies. In this study, diseased guppies (Poecilia reticulata) were collected from an ornamental fish market, and scuticociliate Philaster apodigitiformis Miao et al., 2009 was isolated. In our prior transcriptome sequencing research, we discovered significant expression of the β-PKA gene in P. apodigitiformis during its infection process, leading us to speculate its involvement in pathogenesis. A complete sequence of the β-PKA gene was cloned, and quantified by quantitative reverse transcription-polymerase chain reaction to analyse or to evaluate the functional characteristics of the β-PKA gene. Morphological identification and phylogenetic analysis based on small subunit rRNA sequence, infection experiments and haematoxylin–eosin staining method were also carried out, in order to study the pathological characteristics and infection mechanism of scuticociliate. The present results showed that: (1) our results revealed that β-PKA is a crucial gene involved in P. apodigitiformis infection in guppies, and the findings provide valuable insights for future studies on scuticociliatosis; (2) we characterized a complete gene, β-PKA, that is generally expressed in parasitic organisms during infection stage and (3) the present study indicates that PKA plays a critical role in scuticociliate when infection occurs by controlling essential steps such as cell growth, development and regulating the activity of the sensory body structures and the irritability system.

Extreme heat events in the summer of 2022 were observed in Eurasia, North America and China. Glaciers are a unique indicator of climate change, and the European Alps experienced substantial glacier mass loss as a result of the conditions in 2022, which prompted a wide range of community concerns. However, relevant findings for glaciers in China have not been currently reported. Here, we document the response of Urumqi Glacier No. 1 in the eastern Tien Shan to the extreme heat observed in 2022 based on in situ measurements that span more than 60 years. In 2022, Urumqi Glacier No. 1 exhibited the second largest annual mass loss on record, and the summer mass balance was the most negative on record. The hottest summer on record and relatively lower solid precipitation ratio contributed to the exceptional mass losses at Urumqi Glacier No. 1 in 2022, demonstrating the significant influence of heatwaves on extreme glacier melt in China.