The effects of monolaurin (ML) on the health of piglets infected with porcine epidemic diarrhoea virus (PEDV) have not been fully understood. This study aimed to investigate its role in blood biochemical profile, intestinal barrier function, antioxidant function and the expression of antiviral genes in piglets infected with PEDV. Thirty-two piglets were randomly divided into four groups: control group, ML group, PEDV group and ML + PEDV group. Piglets were orally administrated with ML at a dose of 100 mg/kg·BW for 7 d before PEDV infection. Results showed that PEDV infection significantly decreased D-xylose content and increased intestinal fatty acid-binding protein content, indicating that PEDV infection destroyed intestinal barrier and absorption function. While it could be repaired by ML administration. Moreover, ML administration significantly decreased plasma blood urea nitrogen and total protein content upon PEDV infection. These results suggested ML may increase protein utilisation efficiency. ML administration significantly decreased the number of large unstained cells and Hb and increased the number of leucocytes and eosinophils in the blood of PEDV-infected piglets, indicating ML could improve the immune defense function of the body. In the presence of PEDV infection, ML administration significantly increased superoxide dismutase and catalase activities in blood and colon, respectively, indicating ML could improve antioxidant capacity. Besides, ML administration reversed the expression of ISG15, IFIT3 and IL-29 throughout the small intestine and Mx1 in jejunum and ileum, indicating the body was in recovery from PEDV infection. This study suggests that ML could be used as a kind of feed additive to promote swine health upon PEDV infection.

The advent of time-domain sky surveys has generated a vast amount of light variation data, enabling astronomers to investigate variable stars with large-scale samples. However, this also poses new opportunities and challenges for the time-domain research. In this paper, we focus on the classification of variable stars from the Catalina Surveys Data Release 2 and propose an imbalanced learning classifier based on Self-paced Ensemble (SPE) method. Compared with the work of Hosenie et al. (2020), our approach significantly enhances the classification Recall of Blazhko RR Lyrae stars from 12% to 85%, mixed-mode RR Lyrae variables from 29% to 64%, detached binaries from 68% to 97%, and LPV from 87% to 99%. SPE demonstrates a rather good performance on most of the variable classes except RRab, RRc, and contact and semi-detached binary. Moreover, the results suggest that SPE tends to target the minority classes of objects, while Random Forest is more effective in finding the majority classes. To balance the overall classification accuracy, we construct a Voting Classifier that combines the strengths of SPE and Random Forest. The results show that the Voting Classifier can achieve a balanced performance across all classes with minimal loss of accuracy. In summary, the SPE algorithm and Voting Classifier are superior to traditional machine learning methods and can be well applied to classify the periodic variable stars. This paper contributes to the current research on imbalanced learning in astronomy and can also be extended to the time-domain data of other larger sky survey projects (LSST, etc.).

This paper provides nonparametric specification tests for the commonly used homogeneous and stable coefficients structures in panel data models. We first obtain the augmented residuals by estimating the model under the null hypothesis and then run auxiliary time series regressions of augmented residuals on covariates with time-varying coefficients (TVCs) via sieve methods. The test statistic is then constructed by averaging the squared fitted values, which are close to zero under the null and deviate from zero under the alternatives. We show that the test statistic, after being appropriately standardized, is asymptotically normal under the null and under a sequence of Pitman local alternatives. A bootstrap procedure is proposed to improve the finite sample performance of our test. In addition, we extend the procedure to test other structures, such as the homogeneity of TVCs or the stability of heterogeneous coefficients. The joint test is extended to panel models with two-way fixed effects. Monte Carlo simulations indicate that our tests perform reasonably well in finite samples. We apply the tests to re-examine the environmental Kuznets curve in the United States, and find that the model with homogenous TVCs is more appropriate for this application.

Although ethanol treatment is widely used to activate oocytes, the underlying mechanisms are largely unclear. Roles of intracellular calcium stores and extracellular calcium in ethanol-induced activation (EIA) of oocytes remain to be verified, and whether calcium-sensing receptor (CaSR) is involved in EIA is unknown. This study showed that calcium-free ageing (CFA) in vitro significantly decreased intracellular stored calcium (sCa) and CaSR expression, and impaired EIA, spindle/chromosome morphology and developmental potential of mouse oocytes. Although EIA in oocytes with full sCa after ageing with calcium does not require calcium influx, calcium influx is essential for EIA of oocytes with reduced sCa after CFA. Furthermore, the extremely low EIA rate in oocytes with CFA-downregulated CaSR expression and the fact that inhibiting CaSR significantly decreased the EIA of oocytes with a full complement of CaSR suggest that CaSR played a significant role in the EIA of ageing oocytes. In conclusion, CFA impaired EIA and the developmental potential of mouse oocytes by decreasing sCa and downregulating CaSR expression. Because mouse oocytes routinely treated for activation (18 h post hCG) are equipped with a full sCa complement and CaSR, the present results suggest that, while calcium influx is not essential, CaSR is required for the EIA of oocytes.

There are few studies on the intelligent guidance of unmanned sailboats, which should coordinate pluralistic tasks at sea in the nature of its maneuvring intractability. To ensure the algorithmic practicability, this paper proposes a path-following and collision-avoidance guidance approach of unmanned sailboats with total formulaic description. The risk-detecting mechanism is fabricated by setting a circular detecting zone and using the time to the closest point of approach. Then, the risk of collision, the path deviation, the speed loss, and the course loss can be judged by constructing the cost functions and applying the distance to closest point of approach. The optimized heading angle is deemed as the one minimizing the aggregate cost functions, which is sought by applying and improving the beetle antennae search (BAS) algorithm. In the proposed modified BAS, the searching step is redesigned to enhance the searching efficiency. To ensure the convergence of the real heading angle to the reference, the backstepping-based control law is fabricated for the high-order sailboat model and in the linear form. The control parameters are offline optimized through the modified BAS. Compared with the adaptive control, this controller can guarantee more computation simplicity and the optimized control performance. Finally, simulation corroborates that the sailboat can successfully complete path following and collision avoidance while encountering multiple static and moving obstacles under the proposed schemes.

In this paper, the reflection of curved shock waves over a symmetry plane in planar supersonic flow is studied. This includes stable Mach reflection (MR) and the regular reflection (RR) to MR transition process. Curved shock theory (CST) is applied to derive the high-order parameters in front of and behind the shock wave. The method of curved shock characteristics is used to establish an analytical model to predict the wave configurations. The shock structures provided by the proposed model agree well with the numerical results. Flow structures, such as the height of the Mach stem and the shape of the shock wave and slip line, are studied by applying the analytical model. Isentropic waves generated from a curved wall are found to significantly influence the flow patterns. It appears that the compression waves obstruct the formation of the sonic throat and increase the Mach-stem height. The expansion waves have the opposite effect. The evolution mechanism of the Mach stem is found in conjunction with the RR-to-MR transition process. The CST is extended to a moving frame and used to model the transition. The time history of the moving triple point illustrates the effects of the incident shock angle and isentropic waves on the transition process.

High-speed water entry is a transient hydrodynamic process that is accompanied by strongly compressible flow, free surface splash, cavity evolution and other nonlinear hydrodynamic phenomena. To address these problems, a novel fluid–structure interaction (FSI) scheme based on the immersed boundary method is proposed which is suitable for strongly compressible multiphase flows. In this scheme, considering the multiphase interfaces at the immersed boundary, an improved immersed boundary method for effectively suppressing the non-physical force oscillation is proposed. Additionally, a quaternion-based six degrees of freedom motion system is used to describe rigid body motion, and the multiphase flow Eulerian finite element method is applied as the fluid solver. Using analytical solutions, experimental data and literature data, the accuracy and robustness of the FSI scheme are validated. Finally, the high-speed water entry of the slender body with different noses is investigated, and the hydrodynamic loads including the axial and normal drag forces and the bending moment are extensively discussed. The hydrodynamic load and motion trajectory are determined by the nose configuration. The tail slamming phenomenon is the primary focus, and it is revealed that its formation is primarily related to the pitch moment formed at the stage of crossing the free surface. Tail slamming also causes violent impact loads, especially bending moments, which may cause slender projectiles to break off. Finally, to combine the features of the flat and hemispherical noses, the water entry of the projectile with a truncated hemispherical nose is simulated and discussed.

Parametric interaction allows both forward and backward energy transfers among the three interacting waves. The back-conversion effect is usually detrimental when unidirectional energy transfer is desired. In this theoretical work, we manifest that the back-conversion effect underpins the direct generation of the picosecond pulse train without the need for a laser resonator. The research scenario is an optical parametric amplification (OPA) that consists of a second-order nonlinear medium, a quasi-continuous pump laser and a sinusoidal amplitude-modulated seed signal. The back-conversion of OPA can transfer the modulation peaks (valleys) of the incident signal into output valleys (peaks), which inherently induces spectral sidebands. The generation of each sideband is naturally accompanied with a phase shift of ±π. In the regime of full-back-conversion, the amount and amplitude of the sidebands reach the maximum simultaneously, and their phase constitutes an arithmetic sequence, leading to the production of a picosecond pulse train. The generated picosecond pulse train can have an ultrahigh repetition rate of 40 GHz or higher, which may facilitate ultrafast applications with ultrahigh speed.

Ovarian cancer is the most lethal female reproductive system tumour. Despite the great advances in surgery and systemic chemotherapy over the past two decades, almost all patients in stages III and IV relapse and develop resistance to chemotherapy after first-line treatment. Ovarian cancer has an extraordinarily complex immunosuppressive tumour microenvironment in which immune checkpoints negatively regulate T cells activation and weaken antitumour immune responses by delivering immunosuppressive signals. Therefore, inhibition of immune checkpoints can break down the state of immunosuppression. Indeed, Immune checkpoint inhibitors (ICIs) have revolutionised the therapeutic landscape of many solid tumours. However, ICIs have yielded modest benefits in ovarian cancer. Therefore, a more comprehensive understanding of the mechanistic basis of the immune checkpoints is needed to improve the efficacy of ICIs in ovarian cancer. In this review, we systematically introduce the mechanisms and expression of immune checkpoints in ovarian cancer. Moreover, this review summarises recent updates regarding ICI monotherapy or combined with other small-molecule-targeted agents in ovarian cancer.

The northern Alxa region is located in the central segment of the southern Central Asian Orogenic Belt. Many controversies and deficiencies still exist regarding the magma source characteristics, petrogenesis and tectonic regimes during the late Palaeozoic – early Mesozoic period within this region. This study presents whole-rock compositions and zircon U–Pb and Lu–Hf isotopic data for three early Mesozoic I- and A-type granitic plutons occurring in the northern Alxa region. The Haerchaoenji and Chahanhada I-type granitoids yielded zircon 206Pb–238U ages of 245 ± 5 Ma and 245 ± 2 Ma, respectively. The variable positive zircon ϵHf(t) values between +1.8 and +11.8, with young TDM ages of 425–837 Ma, indicate that these I-type granitoids were mainly derived from juvenile crustal materials. The Wulantaolegai pluton has a zircon 206Pb–238U age of 237 ± 2 Ma and is classified as having high-K calc-alkaline A-type affinity. Furthermore, the positive zircon ϵHf(t) values of the Wulantaolegai granite range from +3.3 to +8.7 with young TDM ages of 545–778 Ma, suggesting the involvement of a juvenile crustal source as well. Furthermore, the major-element compositions of the Chahanhada and Wulantaolegai granites suggest the input of metasedimentary components. Geochemically, the Haerchaoenji and Chahanhada I-type granitoids show an arc affinity, while the Wulantaolegai granite exhibits a post-collisional affinity. However, with regional data, we suggest that the Haerchaoenji and Chahanhada I-type granitoids were also emplaced in a post-collisional setting, and the arc affinity was probably inherited from recycled subduction-related materials. These lines of evidence obtained in this study enable us to argue that the Palaeo-Asian Ocean in the central segment of the Central Asian Orogenic Belt closed before Middle Triassic time.